The impact of the E46K mutation on the properties of alpha-synuclein in its monomeric and oligomeric states.

abstract：:Ammonia is an inhibitor of water oxidation and a structural analogue for substrate water, making it a valuable probe for the structural properties of the possible substrate-binding site on the oxygen-evolving complex (OEC) in photosystem II (PSII). By using the NH(3)-induced upshift of the 1365 cm(-)(1) IR mode in the...

journal_title：Biochemistry

authors： Fang CH,Chiang KA,Hung CH,Chang K,Ke SC,Chu HA

更新日期：2005-07-19 00:00:00

abstract：:The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...

journal_title：Biochemistry

authors： Dwulet FE,Bogardt RA,Jones BN,Lehman LD,Gurd FR

更新日期：1975-12-02 00:00:00

abstract：:The electronic (ultraviolet) circular dichroism (UVCD) and vibrational circular dichroism (VCD) of 20 proteins are systematically compared as to their relationship to the secondary structures of these proteins. The UVCD spectra are statistically treated by use of the same factor analysis methods used previously for VC...

journal_title：Biochemistry

authors： Pancoska P,Keiderling TA

更新日期：1991-07-16 00:00:00

abstract：:Ribonuclease P2 from the thermophilic archaebacterium Sulfolobus solfataricus is a small protein (7 kDa) with a known three-dimensional structure. Inspection of the structure and molecular dynamics simulation reveal that three aromatic residues (Phe5, Phe31, and Tyr33) from the hydrophobic core have a strong van der W...

journal_title：Biochemistry

authors： Mombelli E,Afshar M,Fusi P,Mariani M,Tortora P,Connelly JP,Lange R

更新日期：1997-07-22 00:00:00

abstract：:PII proteins are sensors of alpha-ketoglutarate and adenylylate energy charge that regulate signal transduction proteins, metabolic enzymes, and permeases involved in nitrogen assimilation. Here, purified Escherichia coli PII and two of its receptors, ATase and NRII, were used to study the mechanisms of sensation by P...

journal_title：Biochemistry

authors： Jiang P,Ninfa AJ

更新日期：2009-12-08 00:00:00

abstract：:Slow conformational changes have been proposed to be responsible for the kinetic positive cooperativity of glucokinase (GK) with glucose. Induced-fit and preexisting equilibrium kinetic models have been previously suggested. In the present study, equilibrium and pre-steady-state fluorescence spectroscopy has been used...

journal_title：Biochemistry

authors： Antoine M,Boutin JA,Ferry G

更新日期：2009-06-16 00:00:00

abstract：:We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...

journal_title：Biochemistry

authors： Bucci E,Fronticelli C,Gryczynski Z

更新日期：1991-04-02 00:00:00

abstract：:The molecular dynamics of binary dispersions of plasmenylcholine/cholesterol and phosphatidylcholine/cholesterol were quantified by electron spin resonance (ESR) and deuterium magnetic resonance (2H NMR) spectroscopy. The order parameter of both 5-doxylstearate (5DS) and 16-doxylstearate (16DS) was larger in vesicles ...

journal_title：Biochemistry

authors： Pak JH,Bork VP,Norberg RE,Creer MH,Wolf RA,Gross RW

更新日期：1987-07-28 00:00:00

abstract：:It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...

journal_title：Biochemistry

authors： Goto Y,Hagihara Y

更新日期：1992-01-28 00:00:00

abstract：:The crystal structure of a human carbonic anhydrase II (CAII) variant, cis-proline-202-->alanine (P202A), has been determined at 1.7-A resolution, indicating that the wild-type geometry, including the cis-peptidyl linkage, is retained upon substitution of proline by alanine. The CO2 hydrase activity and affinity for s...

journal_title：Biochemistry

authors： Tweedy NB,Nair SK,Paterno SA,Fierke CA,Christianson DW

更新日期：1993-10-19 00:00:00

abstract：:In an irreversible enzyme-catalyzed reaction, strong binding of the products would lead to substantial product inhibition. The X-ray crystal structure of the product complex of thymidylate synthase (1.83-A resolution, R factor = 0.183 for all data between 7.0 and 1.83 A) identifies a bound water molecule that serves t...

journal_title：Biochemistry

authors： Fauman EB,Rutenber EE,Maley GF,Maley F,Stroud RM

更新日期：1994-02-15 00:00:00

abstract：:The microscopic interactions and mechanisms leading to nascent protein folding events are generally unknown. While such short time-scale events are difficult to study experimentally, molecular dynamics simulations of peptides can provide a useful model for studying events related to protein folding initiation. Recentl...

journal_title：Biochemistry

authors： Karpen ME,Tobias DJ,Brooks CL 3rd

更新日期：1993-01-19 00:00:00

abstract：:The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...

journal_title：Biochemistry

authors： Rheinnecker M,Baker G,Eder J,Fersht AR

更新日期：1993-02-09 00:00:00

abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

journal_title：Biochemistry

authors： Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

更新日期：1985-04-09 00:00:00

abstract：:We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as...

journal_title：Biochemistry

authors： Zimányi L,Lanyi JK

更新日期：1989-06-13 00:00:00

abstract：:A gene encoding the high-potential iron-sulfur protein (HiPIP) was cloned from the purple photosynthetic bacterium Rubrivivax gelatinosus. An insertional disruption of this gene by a kanamycin resistance cartridge resulted in a significant decrease in the growth rate under photosynthetic growth conditions. Flash-induc...

journal_title：Biochemistry

authors： Nagashima KV,Matsuura K,Shimada K,Verméglio A

更新日期：2002-11-26 00:00:00

abstract：:The beta(2) subunit of a class Ia or Ib ribonucleotide reductase (RNR) is activated when its carboxylate-bridged Fe(2)(II/II) cluster reacts with O(2) to oxidize a nearby tyrosine (Y) residue to a stable radical (Y(*)). During turnover, the Y(*) in beta(2) is thought to reversibly oxidize a cysteine (C) in the alpha(2...

journal_title：Biochemistry

authors： Jiang W,Yun D,Saleh L,Bollinger JM Jr,Krebs C

更新日期：2008-12-30 00:00:00

abstract：:Kinetic and spectroscopic studies have shown that the conserved active site residue His200 of the extradiol ring-cleaving homoprotocatechuate 2,3-dioxygenase (FeHPCD) from Brevibacterium fuscum is critical for efficient catalysis. The roles played by this residue are probed here by analysis of the steady-state kinetic...

journal_title：Biochemistry

authors： Kovaleva EG,Rogers MS,Lipscomb JD

更新日期：2015-09-01 00:00:00

abstract：:The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

journal_title：Biochemistry

authors： Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

更新日期：2010-01-26 00:00:00

abstract：:The human LD78 protein (sometimes referred to as human macrophage inflammatory protein-1 alpha) has been shown to protect multipotential hemopoietic stem cells from the effects of cytotoxic agents. Administration of the recombinant stem cell inhibitor molecule LD78 as an adjunct to chemotherapy has potential clinical ...

journal_title：Biochemistry

authors： Patel SR,Evans S,Dunne K,Knight GC,Morgan PJ,Varley PG,Craig S

更新日期：1993-05-25 00:00:00

abstract：:Hydroxyl radical induced formation of a DNA-protein cross-link involving thymine and tyrosine in nucleohistone is described. Hydroxyl radicals were generated in N2O-saturated aqueous solution by ionizing radiation. Samples of nucleohistone were hydrolyzed with HCl and trimethylsilylated. Analysis of irradiated samples...

journal_title：Biochemistry

authors： Dizdaroglu M,Gajewski E,Reddy P,Margolis SA

更新日期：1989-04-18 00:00:00

abstract：:The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...

journal_title：Biochemistry

authors： Chen H,Bernstein BW,Sneider JM,Boyle JA,Minamide LS,Bamburg JR

更新日期：2004-06-08 00:00:00

abstract：:Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...

journal_title：Biochemistry

authors： Hamilton SE,Pitts AE,Katipally RR,Jia X,Rutter JP,Davies BA,Shay JW,Wright WE,Corey DR

更新日期：1997-09-30 00:00:00

abstract：:When the single-stranded RNA genome of HIV-1 is copied into double-stranded DNA, the viral enzyme reverse transcriptase (RT) catalyzes the addition of approximately 20,000 nucleotides; however, the precise mechanism of nucleotide addition is unknown. In this study, we attempt to integrate the genetic data and biochemi...

journal_title：Biochemistry

authors： Patel PH,Jacobo-Molina A,Ding J,Tantillo C,Clark AD Jr,Raag R,Nanni RG,Hughes SH,Arnold E

更新日期：1995-04-25 00:00:00

abstract：:Intestinal fatty acid binding protein (IFABP) and liver FABP (LFABP), homologous proteins expressed at high levels in intestinal absorptive cells, employ markedly different mechanisms for the transfer of fatty acids (FAs) to acceptor membranes. Transfer from IFABP occurs during protein-membrane collisional interaction...

journal_title：Biochemistry

authors： Córsico B,Liou HL,Storch J

更新日期：2004-03-30 00:00:00

abstract：:A previously unknown polypeptide myotoxin, designated myotoxin a, was isolated for the first time from prairie rattlesnake (Crotalus viridis viridis) venom. Electrophoretic homogeneity of myotoxin a was shown in beta-alanine disc gel polyacrylamide gel electrophoresis and in isoelectric focusing gel electrophoresis. M...

journal_title：Biochemistry

authors： Cameron DL,Tu AT

更新日期：1977-05-31 00:00:00

abstract：:Transcription of the human nonmuscle myosin II heavy chain-A (NMHC-A) gene is regulated via multiple elements located in intron 1, including element F which contains an E-box. In this study we have identified and characterized the factors that are capable of binding to element F. Yeast one-hybrid screening using eleme...

journal_title：Biochemistry

authors： Chung MC,Kim HK,Kawamoto S

更新日期：2001-07-31 00:00:00

abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...

journal_title：Biochemistry

authors： Hazra S,Szewczak A,Ort S,Konrad M,Lavie A

更新日期：2011-04-12 00:00:00

abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...

journal_title：Biochemistry

authors： Nicely NI,Parsonage D,Paige C,Newton GL,Fahey RC,Leonardi R,Jackowski S,Mallett TC,Claiborne A

更新日期：2007-03-20 00:00:00

abstract：:Campylobacter jejuni contains a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier and subsequently transferred to the asparagine side chain of an acceptor protein. The enzymes in the pathway function at a membrane interface and have in ...

journal_title：Biochemistry

authors： Chen MM,Weerapana E,Ciepichal E,Stupak J,Reid CW,Swiezewska E,Imperiali B

更新日期：2007-12-18 00:00:00