当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Transient spectroscopy of bacterial rhodopsins with an optical multichannel analyzer. 2. Effects of anions on the halorhodopsin photocycle.

Transient spectroscopy of bacterial rhodopsins with an optical multichannel analyzer. 2. Effects of anions on the halorhodopsin photocycle.

Abstract：

:We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as HRhv----HR'K----HRKO----HRO----HR, seems characteristic of what one would observe in the absence of chloride. Absorption spectra are calculated for all species but HRK and HR'K, which occur at shorter times (less than 60 ns) than we can resolve. At nitrate concentrations between 0.1 and 1 M, the proportion of HR which enters the first kind of photocycle increases in such a way as to suggest that nitrate can substitute for chloride, but much less effectively. At lower anion concentrations, the two photocycles are independent of one another, but at higher concentrations, they interact; i.e., the reaction HRKO----HRO----HRL can be observed. Thus, HRO must be common to the two photocycles. Kinetic fitting of the time dependence of HRL and HRO at different chloride concentrations provides evidence for the participation of chloride in the interconversion of HRL and HRO. The results are consistent with a model in which the photoreaction is influenced by the binding of an anion (either chloride or nitrate) to site II in HR: when an anion is bound, the HRK-initiated HRL-type photocycle is observed, but when the site is not occupied, the HR'K-initiated HRO-type photocycle is seen.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Zimányi L,Lanyi JK

doi

10.1021/bi00438a039

subject

Has Abstract

pub_date

1989-06-13 00:00:00

pages

5172-8

issue

12

eissn

0006-2960

issn

1520-4995

journal_volume

28

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • NmerA of Tn501 mercuric ion reductase: structural modulation of the pKa values of the metal binding cysteine thiols.

    abstract：:To avoid nonspecific and/or undesirable binding and reactivity of metal ions with cellular components, organisms have evolved metal-specific systems for trafficking proteins. Although systems differ, those handling soft metal ions such as Hg(2+), Cu(+), Zn(2+), etc., all utilize heavy metal-associated (HMA) proteins a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100537f

    authors： Ledwidge R,Hong B,Dötsch V,Miller SM

    更新日期：2010-10-19 00:00:00

  • A three-dimensional molecular model of lipid-free apolipoprotein A-I determined by cross-linking/mass spectrometry and sequence threading.

    abstract：:Apolipoprotein (apo) A-I, a 243-residue, 28.1-kDa protein is a major mediator of the reverse cholesterol transport (RCT) pathway, a process that may reduce the risk of cardiovascular disease in humans. In plasma, a small fraction of lipid-free or lipid-poor apoA-I is likely a key player in the first step of RCT. There...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047717+

    authors： Silva RA,Hilliard GM,Fang J,Macha S,Davidson WS

    更新日期：2005-03-01 00:00:00

  • NH3 Binding to the S2 State of the O2-Evolving Complex of Photosystem II: Analogue to H2O Binding during the S2 → S3 Transition.

    abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00974

    authors： Askerka M,Vinyard DJ,Brudvig GW,Batista VS

    更新日期：2015-09-29 00:00:00

  • Mutational analysis of the role of the N terminus of actin in actomyosin interactions. Comparison with other mutant actins and implications for the cross-bridge cycle.

    abstract：:Yeast actin mutants with acidic residues at the N terminus either neutralized (DNEQ) or deleted (delta-DSE) were used to assess the role of N-terminal acidic residues in the interactions of actin with myosin in the contractile cycle. Cosedimentation experiments revealed an approximately 3-fold decrease in the binding ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962388+

    authors： Miller CJ,Wong WW,Bobkova E,Rubenstein PA,Reisler E

    更新日期：1996-12-24 00:00:00

  • Structural insights into the two sequential folding transition states of the PB1 domain of NBR1 from Φ value analysis and biased molecular dynamics simulations.

    abstract：:The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1016793

    authors： Chen P,Evans CL,Hirst JD,Searle MS

    更新日期：2011-01-11 00:00:00

  • Ubiquitination-dependent proteolysis of O6-methylguanine-DNA methyltransferase in human and murine tumor cells following inactivation with O6-benzylguanine or 1,3-bis(2-chloroethyl)-1-nitrosourea.

    abstract：:In this study, we investigated the role of ubiquitination in the disposition of the inactivated O6-methylguanine-DNA methyltransferase (MGMT) protein in human (HT-29 and CEM) and murine (ts85) tumor cells. Using a combination of immunoprecipitation and immunoblotting techniques with antibodies against ubiquitin and MG...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9518205

    authors： Srivenugopal KS,Yuan XH,Friedman HS,Ali-Osman F

    更新日期：1996-01-30 00:00:00

  • Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.

    abstract：:Seventeen ribosomal proteins of Saccharomyces cerevisiae were isolated from small ribosomal subunits and disodium ethylenediaminetetraacetate treated 80S ribosomes by chromatography on a column of carboxymethylcellulose and/or by filtration through Sephacryl S-200. The isolated proteins are YS4, YS7, YS8, YS9, YS10, Y...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00262a005

    authors： Otaka E,Higo K,Osawa S

    更新日期：1982-09-14 00:00:00

  • Xenopus laevis hemoglobin and its hybrids with hemoglobin A+.

    abstract：:Isolated alpha and beta chains from Xenopus laevis hemoglobin have been purified. The isolation procedure yields native alpha chains whose functional behavior has been characterized and compared with that of human alpha chains. Isolated beta chains in the presence of oxygen are characterized by low stability, and henc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00395a022

    authors： Condò SG,Giardina B,Bellelli A,Brunori M

    更新日期：1987-10-20 00:00:00

  • Stability effects of increasing the hydrophobicity of solvent-exposed side chains in staphylococcal nuclease.

    abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9725069

    authors： Schwehm JM,Kristyanne ES,Biggers CC,Stites WE

    更新日期：1998-05-12 00:00:00

  • Partitioning of fish and insect antifreeze proteins into ice suggests they bind with comparable affinity.

    abstract：:Antifreeze proteins (AFPs) inhibit the growth of ice by binding to the surface of ice crystals, preventing the addition of water molecules to cause a local depression of the freezing point. AFPs from insects are much more effective at depressing the freezing point than fish AFPs. Here, we have investigated the possibi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035605x

    authors： Marshall CB,Tomczak MM,Gauthier SY,Kuiper MJ,Lankin C,Walker VK,Davies PL

    更新日期：2004-01-13 00:00:00

  • Membrane fusion and inverted phases.

    abstract：:We have found a correlation between liposome fusion kinetics and lipid phase behavior for several inverted phase forming lipids. N-Methylated dioleoylphosphatidylethanolamine (DOPE-Me), or mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC), will form an inverted hexagonal phase ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a011

    authors： Ellens H,Siegel DP,Alford D,Yeagle PL,Boni L,Lis LJ,Quinn PJ,Bentz J

    更新日期：1989-05-02 00:00:00

  • N-ethylmaleimide inhibits Ncd motor function by modification of a cysteine in the stalk domain.

    abstract：:N-Ethylmaleimide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activity of the microtubule (MT) motors kinesin, Ncd, and dynein. Currently, the mechanism of inhibition is not known for any of these proteins. To investigate the mechanism by which NEM inhibits Ncd, the recombi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990706+

    authors： Phelps KK,Walker RA

    更新日期：1999-08-17 00:00:00

  • Steady-state FTIR spectra of the photoreduction of QA and QB in Rhodobacter sphaeroides reaction centers provide evidence against the presence of a proposed transient electron acceptor X between the two quinones.

    abstract：:In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700297b

    authors： Breton J

    更新日期：2007-04-17 00:00:00

  • Switching catalysis from hydrolysis to perhydrolysis in Pseudomonas fluorescens esterase.

    abstract：:Many serine hydrolases catalyze perhydrolysis, the reversible formation of peracids from carboxylic acids and hydrogen peroxide. Recently, we showed that a single amino acid substitution in the alcohol binding pocket, L29P, in Pseudomonas fluorescens (SIK WI) aryl esterase (PFE) increased the specificity constant of P...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9021268

    authors： Yin DL,Bernhardt P,Morley KL,Jiang Y,Cheeseman JD,Purpero V,Schrag JD,Kazlauskas RJ

    更新日期：2010-03-09 00:00:00

  • Monofunctional chorismate mutase from Bacillus subtilis: kinetic and 13C NMR studies on the interactions of the enzyme with its ligands.

    abstract：:The interaction of the monofunctional chorismate mutase from Bacillus subtilis with chorismate and prephenate has been studied kinetically and by NMR spectroscopy with 13C specifically labeled substrates. Prephenate dominates the population of enzyme-bound species, and the "off" rate constant (approximately 60 s-1) ob...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00489a051

    authors： Gray JV,Eren D,Knowles JR

    更新日期：1990-09-18 00:00:00

  • Explanation of pre-steady-state kinetics and decreased burst amplitude of HIV-1 reverse transcriptase at sites of modified DNA bases with an additional, nonproductive enzyme-DNA-nucleotide complex.

    abstract：:The majority of pre-steady-state kinetic investigations with HIV-1 reverse transcriptase (HIV-1 RT) have reported substoichiometric bursts (30-50%) of product formation in the initial reaction cycle. By using quantitative amino acid analysis, we have revised the extinction coefficient of the HIV-1 RT heterodimer and s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982163u

    authors： Furge LL,Guengerich FP

    更新日期：1999-04-13 00:00:00

  • The relationship between ligand aggregation and G-quadruplex DNA selectivity in a series of 3,4,9,10-perylenetetracarboxylic acid diimides.

    abstract：:Human telomeres are comprised of d(TTAGGG) repeats that are capable of forming G-quadruplex DNA structures. Ligands that bind to and stabilize these G-quadruplex DNA structures are potential inhibitors of the cancer cell-associated enzyme telomerase. Other potential biological uses of G-quadruplex targeting ligands ha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0263107

    authors： Kern JT,Thomas PW,Kerwin SM

    更新日期：2002-09-24 00:00:00

  • Picosecond infrared study of the photodynamics of carbonmonoxy-cytochrome c oxidase.

    abstract：:Time-resolved infrared (TRIR) techniques have been employed to study the reactions of carbon monoxide with the cytochrome alpha 3-Cu(B) site of cytochrome c oxidase (CcO). The ligation dynamics immediately following photodissociation have been investigated using picosecond TRIR spectroscopy and linear dichroism. The r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00168a015

    authors： Dyer RB,Peterson KA,Stoutland PO,Woodruff WH

    更新日期：1994-01-18 00:00:00

  • Sequence-Dependent Promoter Escape Efficiency Is Strongly Influenced by Bias for the Pretranslocated State during Initial Transcription.

    abstract：:Abortive transcription initiation can be rate-limiting for promoter escape and therefore represents a barrier to productive gene expression. The mechanism for abortive initiation is unknown, but the amount of abortive transcript is known to vary with the composition of the initial transcribed sequence (ITS). Here, we ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00272

    authors： Skancke J,Bar N,Kuiper M,Hsu LM

    更新日期：2015-07-21 00:00:00

  • Eukaryotic Catalase-Peroxidase: The Role of the Trp-Tyr-Met Adduct in Protein Stability, Substrate Accessibility, and Catalysis of Hydrogen Peroxide Dismutation.

    abstract：:Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00831

    authors： Gasselhuber B,Carpena X,Graf MM,Pirker KF,Nicolussi A,Sündermann A,Hofbauer S,Zamocky M,Furtmüller PG,Jakopitsch C,Oostenbrink C,Fita I,Obinger C

    更新日期：2015-09-08 00:00:00

  • Regulation of insulin-regulated membrane aminopeptidase activity by its C-terminal domain.

    abstract：:The development of inhibitors of insulin-regulated aminopeptidase (IRAP), a membrane-bound zinc metallopeptidase, is a promising approach for the discovery of drugs for the treatment of memory loss such as that associated with Alzheimer's disease. There is, however, no consensus in the literature about the mechanism b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101893w

    authors： Ascher DB,Cromer BA,Morton CJ,Volitakis I,Cherny RA,Albiston AL,Chai SY,Parker MW

    更新日期：2011-04-05 00:00:00

  • Hydrolysis of dihydrouridine and related compounds.

    abstract：:Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951577+

    authors： House CH,Miller SL

    更新日期：1996-01-09 00:00:00

  • Structural differences in the two agonist binding sites of the Torpedo nicotinic acetylcholine receptor revealed by time-resolved fluorescence spectroscopy.

    abstract：:The nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata carries two nonequivalent agonist binding sites at the alphadelta and alphagamma subunit interfaces. These sites have been characterized by time-resolved fluorescence with the partial nicotinic agonist dansyl-C(6)-choline (Dnscho). When bound to the d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992811p

    authors： Martinez KL,Corringer PJ,Edelstein SJ,Changeux JP,Mérola F

    更新日期：2000-06-13 00:00:00

  • Identification of 9-cis,13-cis-retinoic acid as a major circulating retinoid in plasma.

    abstract：:In the course of measuring the concentrations of retinoic acids (RA) in bovine plasma, a major peak was observed which comigrated with 9-cis-RA on normal-phase high-performance liquid chromatography. Rechromatography of this retinoic on reverse-phase high-performance liquid chromatography showed that it was distinct f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00004a013

    authors： Horst RL,Reinhardt TA,Goff JP,Nonnecke BJ,Gambhir VK,Fiorella PD,Napoli JL

    更新日期：1995-01-31 00:00:00

  • Decay of activated Bacillus subtilis pho response regulator, PhoP approximately P, involves the PhoR approximately P intermediate.

    abstract：:PhoR of Bacillus subtilis is a histidine sensor-kinase belonging to the family of two-component signal transduction systems. PhoR is responsible for processing the phosphate-starvation signal and providing phosphate input to regulate the level of phosphorylated response regulator, PhoP, which activates/represses Pho r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990658t

    authors： Shi L,Liu W,Hulett FM

    更新日期：1999-08-03 00:00:00

  • Solution structure of the first intracellular loop of prostacyclin receptor and implication of its interaction with the C-terminal segment of G alpha s protein.

    abstract：:The amino acids (residues 39-51) responsible for the interaction between the first intracellular loop (iLP1) of the human prostacyclin receptor (IP) and G alpha s protein have been identified [Zhang, L., Huang, G., Wu, J., and Ruan, K. H. (2005) Biochemistry 44, 11389-11401]. To further characterize the structural/fun...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0515669

    authors： Zhang L,Wu J,Ruan KH

    更新日期：2006-02-14 00:00:00

  • Enzymatic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates.

    abstract：:In this communication, we present data which describe optimum conditions for reverse transcription of large ribonucleic acid (RNA) templates into deoxyribonucleic acid (DNA) transcripts by the avian retrovirus reverse transcriptase in vitro. In contrast to previous studies, we have optimized all of the reaction compon...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00544a019

    authors： Retzel EF,Collett MS,Faras AJ

    更新日期：1980-02-05 00:00:00

  • Kinetic mechanism of serine transacetylase from Salmonella typhimurium.

    abstract：:Serine transacetylase from Salmonella typhimurium was purified to near homogeneity. A complete initial velocity study in both reaction directions suggests that the enzyme catalyzes the conversion of acetyl CoA and L-serine to O-acetyl-L-serine (OAS) and coenzyme A (CoASH) by a ping pong bi bi kinetic mechanism. Initia...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00175a040

    authors： Leu LS,Cook PF

    更新日期：1994-03-08 00:00:00

  • Surface proton donors for the D-pathway of cytochrome c oxidase in the absence of subunit III.

    abstract：:The major proton-transfer pathway into the buried active site of cytochrome c oxidase (CcO) is the D-pathway that begins with the subunit I residue Asp-132 on the inner protein surface (the cytoplasmic surface of the aa3-type CcO of Rhodobacter sphaeroides). Asp-132 is surrounded by residues from both subunits I and I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0605843

    authors： Adelroth P,Hosler J

    更新日期：2006-07-11 00:00:00

  • Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc.

    abstract：:The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein canno...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00038a003

    authors： Murphy ME,Turley S,Kukimoto M,Nishiyama M,Horinouchi S,Sasaki H,Tanokura M,Adman ET

    更新日期：1995-09-26 00:00:00