Transient spectroscopy of bacterial rhodopsins with an optical multichannel analyzer. 2. Effects of anions on the halorhodopsin photocycle.


:We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as HRhv----HR'K----HRKO----HRO----HR, seems characteristic of what one would observe in the absence of chloride. Absorption spectra are calculated for all species but HRK and HR'K, which occur at shorter times (less than 60 ns) than we can resolve. At nitrate concentrations between 0.1 and 1 M, the proportion of HR which enters the first kind of photocycle increases in such a way as to suggest that nitrate can substitute for chloride, but much less effectively. At lower anion concentrations, the two photocycles are independent of one another, but at higher concentrations, they interact; i.e., the reaction HRKO----HRO----HRL can be observed. Thus, HRO must be common to the two photocycles. Kinetic fitting of the time dependence of HRL and HRO at different chloride concentrations provides evidence for the participation of chloride in the interconversion of HRL and HRO. The results are consistent with a model in which the photoreaction is influenced by the binding of an anion (either chloride or nitrate) to site II in HR: when an anion is bound, the HRK-initiated HRL-type photocycle is observed, but when the site is not occupied, the HR'K-initiated HRO-type photocycle is seen.






Zimányi L,Lanyi JK




Has Abstract


1989-06-13 00:00:00












  • A single mutation in cytochrome P450 BM3 changes substrate orientation in a catalytic intermediate and the regiospecificity of hydroxylation.

    abstract::Phenylalanine 87 of Bacillus megaterium cytochrome P450 BM3, a residue close to the heme in the substrate binding pocket, has been replaced by alanine by site-directed mutagenesis. The substitution had no effect on the rate of hydroxylation of laurate and increased the affinity for laurate of both the intact enzyme an...


    pub_type: 杂志文章


    authors: Oliver CF,Modi S,Sutcliffe MJ,Primrose WU,Lian LY,Roberts GC

    更新日期:1997-02-18 00:00:00

  • Complex between a Multicrossover DNA Nanostructure, PX-DNA, and T7 Endonuclease I.

    abstract::Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...


    pub_type: 杂志文章


    authors: Kizer M,Huntress ID,Walcott BD,Fraser K,Bystroff C,Wang X

    更新日期:2019-03-12 00:00:00

  • Kinetic comparison of bovine blood coagulation factors IXa alpha and IXa beta toward bovine factor X.

    abstract::The Vmax/Km (microM -1 min -1.) for bovine factor X activation by bovine factor IXa alpha, in the presence of sufficient [Ca2+] to saturate the initial reaction rate, was 0.007. When factor IXa beta was substituted for factor IXa alpha in this reaction, the Vmax/Km decreased to 0.001, suggesting that factor IXa alpha ...


    pub_type: 杂志文章


    authors: Link RP,Castellino FJ

    更新日期:1983-08-16 00:00:00

  • Recombinant human CNTF receptor alpha: production, binding stoichiometry, and characterization of its activity as a diffusible factor.

    abstract::The primary ligand-binding protein (CNTFR alpha) of the multicomponent receptor for ciliary neurotrophic factor was produced in Escherichia coli. Using novel applications of size-exclusion chromatography and a protein gel-shift assay, we obtained quantitative separation of correctly refolded protein, as well as analyt...


    pub_type: 杂志文章


    authors: Panayotatos N,Everdeen D,Liten A,Somogyi R,Acheson A

    更新日期:1994-05-17 00:00:00

  • Induction of Translational Readthrough across the Thalassemia-Causing Premature Stop Codon in β-Globin-Encoding mRNA.

    abstract::Nonsense mutations that result in premature stop codons in the HBB gene cause β-thalassemia. This disease is characterized by a reduced hemoglobin level due to the lack of β-globin. Compounds that induce translational readthrough across the thalassemia-causing premature stop codon will have therapeutic benefits. Curre...


    pub_type: 杂志文章


    authors: Kar D,Sellamuthu K,Kumar SD,Eswarappa SM

    更新日期:2020-01-14 00:00:00

  • Site-specific mutagenesis of conserved residues within Walker A and B sequences of Escherichia coli UvrA protein.

    abstract::UvrA is the ATPase subunit of the DNA repair enzyme (A)BC excinuclease. The amino acid sequence of this protein has revealed, in addition to two zinc fingers, three pairs of nucleotide binding motifs each consisting of a Walker A and B sequence. We have conducted site-specific mutagenesis, ATPase kinetic analyses, and...


    pub_type: 杂志文章


    authors: Myles GM,Hearst JE,Sancar A

    更新日期:1991-04-23 00:00:00

  • Dependence of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen photoaddition on the conformation of ribonucleic acid.

    abstract::The photoaddition of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A) . poly(U) (double stranded), and poly(U) . poly(A) . poly(U) (triple stranded) were reacted with HMT at differe...


    pub_type: 杂志文章


    authors: Thompson JF,Bachellerie JP,Hall K,Hearst JE

    更新日期:1982-03-16 00:00:00

  • Mode of interaction of aminooxy compounds with sheep liver serine hydroxymethyltransferase.

    abstract::The interaction of aminooxy compounds such as aminooxyacetate (AAA), L-canaline, and hydroxylamine with sheep liver serine hydroxymethyltransferase (EC was studied by absorption spectra and stopped-flow spectrophotometry and compared with the unique feature of interaction of O-amino-D-serine (OADS) with the e...


    pub_type: 杂志文章


    authors: Baskaran N,Prakash V,Savithri HS,Radhakrishnan AN,Appaji Rao N

    更新日期:1989-12-12 00:00:00

  • COUP-TF plays a dual role in the regulation of the ovalbumin gene.

    abstract::The ovalbumin (Ov) gene contains a number of regulatory elements that control its transcriptional activity and restrict expression to avian oviduct. One major regulatory region, the steroid-dependent regulatory element (SDRE), is required for induction by estrogen and corticosterone. Another region, the negative regul...


    pub_type: 杂志文章


    authors: Park HM,Haecker SE,Hagen SG,Sanders MM

    更新日期:2000-07-25 00:00:00

  • Membrane glycopeptides from virus-transformed hamster fibroblasts and the normal counterpart.

    abstract::Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...


    pub_type: 杂志文章


    authors: Glick MC

    更新日期:1979-06-12 00:00:00

  • Induction of alpha-helix in the beta-sheet protein tumor necrosis factor-alpha: thermal- and trifluoroethanol-induced denaturation at neutral pH.

    abstract::The unfolding and refolding of alpha-helical proteins has been extensively studied, demonstrating formation of intermediate structures which retain the native-like alpha-helix but lack the tertiary structure. Studies on the folding of proteins consisting primarily of beta-sheet are interesting since, unlike the alpha-...


    pub_type: 杂志文章


    authors: Narhi LO,Philo JS,Li T,Zhang M,Samal B,Arakawa T

    更新日期:1996-09-03 00:00:00

  • 2D NMR studies and 3D structure of the parallel-stranded duplex oligonucleotide Acrm5-alpha-d(TCTAAACTC)-beta-d(AGATTTGAG) via complete relaxation matrix analysis of the NOE effects and molecular mechanics calculations.

    abstract::The three-dimensional structure of the duplex formed by the association of the unnatural oligonucleotide alpha-d(TCTAAACTC) covalently linked to an acridine derivative (m5Acr) with its natural and parallel complementary sequence beta-d(AGATTTGAG) was investigated by nuclear magnetic resonance spectroscopy and constrai...


    pub_type: 杂志文章


    authors: Guesnet JL,Vovelle F,Thuong NT,Lancelot G

    更新日期:1990-05-22 00:00:00

  • A mechanism for hydroxylation by tyrosine hydroxylase based on partitioning of substituted phenylalanines.

    abstract::The iron-containing enzyme tyrosine hydroxylase catalyzes the hydroxylation of tyrosine to dihydroxyphenylalanine. A series of 4-X-substituted (X = H, F, Br, Cl, CH3, or CH3O) phenylalanines have been characterized as substrates to gain insight into the mechanism of hydroxylation. Multiple hydroxylated products were f...


    pub_type: 杂志文章


    authors: Hillas PJ,Fitzpatrick PF

    更新日期:1996-06-04 00:00:00

  • Simultaneous binding of drugs with different chemical structures to Ca2+-calmodulin: crystallographic and spectroscopic studies.

    abstract::The modulatory action of Ca2+-calmodulin on multiple targets is inhibited by trifluoperazine, which competes with target proteins for calmodulin binding. The structure of calmodulin crystallized with two trifluoperazine molecules is determined by X-ray crystallography at 2.74 A resolution. The X-ray data together with...


    pub_type: 杂志文章


    authors: Vertessy BG,Harmat V,Böcskei Z,Náray-Szabó G,Orosz F,Ovádi J

    更新日期:1998-11-03 00:00:00

  • Water-soluble beta-sheet models which self-assemble into fibrillar structures.

    abstract::Self-assembly of beta-sheet domains resulting in the formation of pathogenic, fibrillar protein aggregates (amyloids) is a characteristic feature of various medical disorders. These include neurodegenerative diseases, such as Alzheimer's, Huntington's, and Creutzfeldt-Jacob's. A significant problem in studying such ag...


    pub_type: 杂志文章


    authors: Janek K,Behlke J,Zipper J,Fabian H,Georgalis Y,Beyermann M,Bienert M,Krause E

    更新日期:1999-06-29 00:00:00

  • Phospholipase Cβ3 Membrane Adsorption and Activation Are Regulated by Its C-Terminal Domains and Phosphatidylinositol 4,5-Bisphosphate.

    abstract::Phospholipase Cβ (PLCβ) enzymes hydrolyze phosphatidylinositol 4,5-bisphosphate to produce second messengers that regulate intracellular Ca2+, cell proliferation, and survival. Their activity is dependent upon interfacial activation that occurs upon localization to cell membranes. However, the molecular basis for how ...


    pub_type: 杂志文章


    authors: Hudson BN,Hyun SH,Thompson DH,Lyon AM

    更新日期:2017-10-17 00:00:00

  • Investigating the cause of the alkaline transition of phytocyanins.

    abstract::The phytocyanins are a family of plant cupredoxins that have been subdivided into the stellacyanins, plantacyanins, and uclacyanins. All of these proteins possess the typical type 1 His(2)Cys equatorial ligand set at their mononuclear copper sites, but the stellacyanins have an axial Gln ligand in place of the weakly ...


    pub_type: 杂志文章


    authors: Harrison MD,Yanagisawa S,Dennison C

    更新日期:2005-03-01 00:00:00

  • NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain.

    abstract::The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide groups of Lys 24 and Gly 83. These are conserved residues in each of the consensus sequences....


    pub_type: 杂志文章


    authors: Lowry DF,Cool RH,Redfield AG,Parmeggiani A

    更新日期:1991-11-12 00:00:00

  • Possibility of shape conformers of the protein inhibitor of the cyclic adenosine monophosphate dependent protein kinase.

    abstract::The heat-stable, protein inhibitor of the cyclic adenosine monophosphate (cAMP) dependent protein kinase [Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E., & Krebs, E (1971a) J. Biol. Chem. 246, 1977-1985] has been purified to homogeneity from rabbit skeletal muscle by preparative electrophoresis. Em...


    pub_type: 杂志文章


    authors: McPherson JM,Whitehouse S,Walsh DA

    更新日期:1979-10-30 00:00:00

  • Catalytic and DNA binding properties of the ogg1 protein of Saccharomyces cerevisiae: comparison between the wild type and the K241R and K241Q active-site mutant proteins.

    abstract::The Ogg1 protein of Saccharomyces cerevisiae belongs to a family of DNA glycosylases and apurinic/apyrimidinic site (AP) lyases, the signature of which is the alpha-helix-hairpin-alpha-helix-Gly/Pro-Asp (HhH-GPD) active site motif together with a conserved catalytic lysine residue, to which we refer as the HhH-GPD/K f...


    pub_type: 杂志文章


    authors: Guibourt N,Castaing B,Van Der Kemp PA,Boiteux S

    更新日期:2000-02-22 00:00:00

  • Effects of DNA binding proteins on DNA methylation in vitro.

    abstract::The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...


    pub_type: 杂志文章


    authors: Kautiainen TL,Jones PA

    更新日期:1985-02-26 00:00:00

  • Assignment of a ligand in stellacyanin by a pulsed electron paramagnetic resonance method.

    abstract::The electron spin echo decay envelope for the blue copper protein, stellacyanin, and for a number of other Cu(II) complexes has been studied. Particular attention was given to the form of the "nuclear modulation" patterns, which show the effects of coupling between the electron spin and the neighboring nuclei. The env...


    pub_type: 杂志文章


    authors: Mims WB,Peisach J

    更新日期:1976-08-24 00:00:00

  • DNA polymerase λ inactivation by oxidized abasic sites.

    abstract::Base excision repair (BER) plays a vital role in maintaining genomic integrity in mammalian cells. DNA polymerase λ (Pol λ) is believed to play a backup role to DNA polymerase β (Pol β) in base excision repair. Two oxidized abasic lesions that are produced by a variety of DNA-damaging agents, including several antitum...


    pub_type: 杂志文章


    authors: Stevens AJ,Guan L,Bebenek K,Kunkel TA,Greenberg MM

    更新日期:2013-02-05 00:00:00

  • Crystal structures of thrombin complexed to a novel series of synthetic inhibitors containing a 5,5-trans-lactone template.

    abstract::The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...


    pub_type: 杂志文章


    authors: Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

    更新日期:1999-06-22 00:00:00

  • Restriction of the lateral motion of band 3 in the erythrocyte membrane by the cytoskeletal network: dependence on spectrin association state.

    abstract::The effects of incubation of erythrocyte ghosts under various conditions (ionic strength or addition of ankyrin, diamines, or ATP) on the lateral motion of band 3 in the membranes were studied by using the fluorescence photobleaching recovery technique. Incubation of ghosts with exogenous ankyrin increased the immobil...


    pub_type: 杂志文章


    authors: Tsuji A,Ohnishi S

    更新日期:1986-10-07 00:00:00

  • Probing inhibitor-induced conformational changes along the interface between tissue factor and factor VIIa.

    abstract::Upon injury of a blood vessel, activated factor VII (FVIIa) forms a high-affinity complex with its allosteric regulator, tissue factor (TF), and initiates blood clotting. Active site-inhibited factor VIIa (FVIIai) binds to TF with even higher affinity. We compared the interactions of FVIIai and FVIIa with soluble TF (...


    pub_type: 杂志文章


    authors: Osterlund M,Owenius R,Carlsson K,Carlsson U,Persson E,Lindgren M,Freskgård PO,Svensson M

    更新日期:2001-08-07 00:00:00

  • Chemical cross-linking of heteromeric glucocorticoid receptors.

    abstract::Glucocorticoid receptors of wild-type and nti ("increased nuclear transfer") mutant S49.1 mouse lymphoma cells exist in extracts under low-salt conditions predominantly as high molecular weight species (Mr greater than or equal to 300,000). These receptor-hormone complexes are unable to bind to DNA. High salt (300 mM ...


    pub_type: 杂志文章


    authors: Rexin M,Busch W,Gehring U

    更新日期:1988-07-26 00:00:00

  • Interfacial properties of the polyheme cytochrome c3 superfamily from Desulfovibrio.

    abstract::In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...


    pub_type: 杂志文章


    authors: Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

    更新日期:1995-09-12 00:00:00

  • Substrate binding and catalytic mechanism in ascorbate peroxidase: evidence for two ascorbate binding sites.

    abstract::The catalytic mechanism of recombinant soybean cytosolic ascorbate peroxidase (rsAPX) and a derivative of rsAPX in which a cysteine residue (Cys32) located close to the substrate (L-ascorbic acid) binding site has been modified to preclude binding of ascorbate [Mandelman, D., Jamal, J., and Poulos, T. L. (1998) Bioche...


    pub_type: 杂志文章


    authors: Lad L,Mewies M,Raven EL

    更新日期:2002-11-19 00:00:00

  • Crystal structure of the catalytic domain of a thermophilic endocellulase.

    abstract::One way to improve the economic feasibility of biomass conversion is to enhance the catalytic efficiency of cellulases through protein engineering. This requires that high-resolution structures of cellulases be available. Here we present the structure of E2cd, the catalytic domain of the thermophilic endocellulase E2 ...


    pub_type: 杂志文章


    authors: Spezio M,Wilson DB,Karplus PA

    更新日期:1993-09-28 00:00:00