Abstract：
:The kinetics of self-association for beef liver glutamate dehydrogenase (EC 1.4.1.3) have been measured by using pressure perturbation in both the time domain and the frequency domain by monitoring scattered light intensity. The kinetic behavior is entirely consistent with the random self-association model proposed by Thusius et al. [Thusius, D., Dessen, P., & Jallon, J. M. (1975) J. Mol. Biol. 92, 413--432]. The activation volume deltaV for association is estimated to be positive, and it is shown that this provides further corroboration of the molecular mechanism advanced by these same authors. A rapid shift in scattered light intensity is attributed to preferential interaction between the phosphate anion and the protein, proceeding with a positive volume change (2--5 mL/mol of phosphate). A description of the instrument developed for this study is also included.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Halvorson HRdoi
10.1021/bi00579a007subject
Has Abstractpub_date
1979-06-12 00:00:00pages
2480-7issue
12eissn
0006-2960issn
1520-4995journal_volume
18pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The influence of DNA base sequence context on the removal of a bulky benzo[a]pyrene diol epoxide-guanine adduct, (+)-trans-B[a]P-N2-dG (G*), by UvrABC nuclease from the thermophilic organism Bacillus caldotenax was investigated. The lesion was flanked by either T or C in otherwise identical complementary 43-mer duplex...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700294k
更新日期：2007-06-12 00:00:00
abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00277a042
更新日期：1983-04-12 00:00:00
abstract：:The flavin reductase (FRED) and isobutylamine N-hydroxylase (IBAH) from Streptomyces viridifaciens constitute a two-component, flavin-dependent monooxygenase system that catalyzes the first step in valanimycin biosynthesis. FRED is an oxidoreductase that provides the reduced flavin to IBAH, which then catalyzes the hy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00679
更新日期：2021-01-12 00:00:00
abstract：:Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30%. The purification involves solubilization of the acetone powder in a Ca2+-containing buffer and subsequent ammonium sulfate fractionation. Gel filtration on Sephadex G-200 and hydrophobic affinity chromatograp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00591a014
更新日期：1979-11-27 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH is required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300863a
更新日期：2012-10-30 00:00:00
abstract：:Acetylcholine receptors have been purified from junctional regions of normal rat diaphragm muscle and from extrajunctional regions of denervated diaphragm. The reaction of purified receptors with [122I]-alpha-bungarotoxin has been investigated by kinetic methods. The toxin-receptor complexes dissociated in a biphasic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00681a008
更新日期：1975-05-20 00:00:00
abstract：:Inhibition of gamma-aminobutyric acid aminotransferase (GABA-AT) increases the concentration of GABA, an inhibitory neurotransmitter in human brain, which could have therapeutic applications for a variety of neurological diseases, including epilepsy. On the basis of studies of several previously synthesized conformati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061592m
更新日期：2006-12-05 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure and relative flexibilities conferred by different flanking sequences on the EcoRI binding site. This approach allowed us to examine unique peaks and subtle changes in the spectra of d(AAAGAATTCTTT)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8015235
更新日期：2009-02-17 00:00:00
abstract：:Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00182a019
更新日期：1994-04-26 00:00:00
abstract：:High-molecular mass thioredoxin reductases (TRs) are pyridine nucleotide disulfide oxidoreductases that catalyze the reduction of the disulfide bond of thioredoxin (Trx). Trx is responsible for reducing multiple protein disulfide targets in the cell. TRs utilize reduced β-nicotinamide adenine dinucleotide phosphate to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400931k
更新日期：2014-01-28 00:00:00
abstract：:Atrazine chlorohydrolase (AtzA) from Pseudomonas sp. ADP initiates the metabolism of the herbicide atrazine by catalyzing a hydrolytic dechlorination reaction to produce hydroxyatrazine. Sequence analysis revealed AtzA to be homologous to metalloenzymes within the amidohydrolase protein superfamily. AtzA activity was ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020415s
更新日期：2002-12-03 00:00:00
abstract：:FKBP12, a small human enzyme, aids protein folding by catalyzing cis-trans isomerization of peptidyl-prolyl bonds, and is involved in cell signaling pathways, calcium regulation, and the immune response. The underlying molecular mechanisms are not fully understood, but it is well-known that aromatic residues in the ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01157
更新日期：2017-01-10 00:00:00
abstract：:The l-lyxonate dehydratase (LyxD) in vitro enzymatic activity and in vivo metabolic function were assigned to members of an isofunctional family within the mandelate racemase (MR) subgroup of the enolase superfamily. This study combined in vitro and in vivo data to confirm that the dehydration of l-lyxonate is the bio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi5004298
更新日期：2014-05-27 00:00:00
abstract：:Proteolytic processing of the transmembrane domain of the amyloid precursor protein (APP) is a key component of Alzheimer's disease pathogenesis. Using C-terminally tagged APP derivatives, we have identified by amino-terminal sequencing a novel cleavage site of APP, at Leu-49, distal to the gamma-secretase site. This ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015794o
更新日期：2002-02-26 00:00:00
abstract：:A systematic 220-MHz proton nuclear magnetic resonance (nmr) study has been made of all common purine and pyrimidine 2'(3')-ribonucleotides in D2O solutions at 20 plus or minus 2 degrees. Spectra for the entire series were measured under similar conditions of concentration, temperature, and ionic strength, thereby fac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00674a013
更新日期：1975-02-11 00:00:00
abstract：:Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050152c
更新日期：2005-06-28 00:00:00
abstract：:The ability of several DNA polymerases to catalyze the template-directed synthesis of duplex oligonucleotides containing a base pair between a nucleotide with anhydrohexitol ring and its natural complement has been investigated. All DNA polymerases were able to accept the chemically synthesized anhydrohexitol triphosp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001297g
更新日期：2000-10-24 00:00:00
abstract：:Sialyltransferase activities, SAT-3 (CMP-NeuAc:nLcOse4Cer alpha 2-3sialyltransferase) and SAT-4 (CMP-NeuAc:GgOse4Cer alpha 2-3sialyltransferase), in Colo 205 cells catalyze the transfer of sialic acid to the terminal galactose of GlcNc-- and GalNAc-containing glycolipid substrates, respectively. Competition kinetic st...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960239l
更新日期：1996-04-23 00:00:00
abstract：:The first use of computer-simulation studies to examine heparin's structure has been reported. The product distributions obtained when porcine mucosal heparins were depolymerized with heparinase have been compared to computer-simulated distributions. The modeled distribution was relatively unaffected by the polydisper...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00347a045
更新日期：1985-12-17 00:00:00
abstract：:In the present study we establish that specific members of the Sp1 family of transcription factors (Sp1 and Sp3) bind to all six GC-rich motifs (elements 1-6) present in the proximal promoter of the human cell cycle inhibitor p21(WAF-1/Cip1) gene. Competition analysis showed that Sp1 and Sp3 bound with high affinity t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026141q
更新日期：2002-10-22 00:00:00
abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00517a002
更新日期：1981-07-07 00:00:00
abstract：:Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901713r
更新日期：2010-04-06 00:00:00
abstract：:First identified on the surface of lymphoids as a type II transmembrane protein, CD38 has now been established to have dual functions not only as a receptor but also as a multifunctional enzyme,catalyzing the synthesis of and hydrolysis of a general calcium messenger molecule, cyclic ADP-ribose(cADPR). The receptorial...
journal_title：Biochemistry
pub_type： 杂志文章
doi：
更新日期：2008-12-30 00:00:00
abstract：:Determining the sequence of bond cleavages, and consequently the nature of intermediates, in enzyme-catalyzed reactions is a major goal of mechanistic enzymology. When significant primary isotope effects on V/K are observed for two different bond cleavages, both bonds may be broken in the same transition state or they...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00238a013
更新日期：1991-06-18 00:00:00
abstract：:The folding of many globular proteins from the unfolded (U) to the native (N) state appears to be describable by a two-state N ↔ U model, which has led to the general belief that protein folding occurs in a highly cooperative manner. One reason for the widespread belief in "two-state folding" is that protein folding r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00698
更新日期：2018-10-09 00:00:00
abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972163e
更新日期：1998-01-06 00:00:00
abstract：:Expression of the PSR132 protein from Dianthus caryophyllus (carnation, clover pink) is induced in response to ethylene production associated with petal senescence, and thus the protein is named petal death protein (PDP). Recent work has established that despite the annotation of PDP in sequence databases as carboxyph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051779y
更新日期：2005-12-20 00:00:00
abstract：:Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701954p
更新日期：2008-01-08 00:00:00
abstract：:2-[(R)-2-Hydroxypropylthio]ethanesulfonate (R-HPC) dehydrogenase (DH) catalyzes the reversible oxidation of R-HPC to 2-(2-ketopropylthio)ethanesulfonate (2-KPC) in a key reaction in the bacterial conversion of chiral epoxides to beta-keto acids. R-HPCDH is highly specific for the R-enantiomer of HPC, while a separate ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049783h
更新日期：2004-06-01 00:00:00
abstract：:Cyanide inhibits the CO oxidation activity of carbon monoxide dehydrogenase from Clostridium thermoaceticum by binding tightly to the form of the C-cluster yielding the gav = 1.82 signal (the C1.82 form). CN- dissociates and the enzyme reactivates upon addition of CO, CO2 plus dithionite, or CS2 plus dithionite. Dithi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00195a011
更新日期：1994-07-26 00:00:00
