An FTIR investigation of flanking sequence effects on the structure and flexibility of DNA binding sites.

abstract：:The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor α (hERα) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II β-turn conformat...

journal_title：Biochemistry

authors： Byrne C,Henen MA,Belnou M,Cantrelle FX,Kamah A,Qi H,Giustiniani J,Chambraud B,Baulieu EE,Lippens G,Landrieu I,Jacquot Y

更新日期：2016-09-27 00:00:00

abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...

journal_title：Biochemistry

authors： Gross A,Hubbell WL

更新日期：2002-01-29 00:00:00

abstract：:Aureochromes have recently been shown to act as blue-light-regulated transcription factors in the stramenopile alga Vaucheria frigida. They comprise a light-, oxygen-, or voltage-sensitive (LOV) domain as a sensory module with flavin mononucleotide (FMN) as a chromophore and a basic region leucine zipper (bZIP) domain...

journal_title：Biochemistry

authors： Herman E,Sachse M,Kroth PG,Kottke T

更新日期：2013-05-07 00:00:00

abstract：:In order to compare the interfacial behavior of the polyheme cytochromes c which belong to the cytochrome c3 superfamily, the monomolecular film technique was used to determine whether and how these metalloproteins interact with (phospho)lipids). Measurements of the variations of surface pressure and surface potential...

journal_title：Biochemistry

authors： Florens L,Ivanova M,Dolla A,Czjzek M,Haser R,Verger R,Bruschi M

更新日期：1995-09-12 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...

journal_title：Biochemistry

authors： Jatana N,Aswin SK,Rathore S,Thukral L

更新日期：2020-01-28 00:00:00

abstract：:We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding...

journal_title：Biochemistry

authors： Boles TC,Hogan ME

更新日期：1986-05-20 00:00:00

abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...

journal_title：Biochemistry

authors： Pudasaini A,Zoltowski BD

更新日期：2013-10-08 00:00:00

abstract：:The kinetics of Zn(2+) binding by two point-mutated forms of the HIV-1 NCp7 C-terminal zinc finger, each containing tridentate binding motif HCC [Ser49(35-50)NCp7] or CCC [Ala44(35-50)NCp7], has been studied by stopped-flow spectrofluorimetry. Both the formation and dissociation rate constants of the complexes between...

journal_title：Biochemistry

authors： Bombarda E,Roques BP,Mély Y,Grell E

更新日期：2005-05-17 00:00:00

abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...

journal_title：Biochemistry

authors： Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

更新日期：1999-03-30 00:00:00

abstract：:In the cyanobacterium Anabaena, the precursor to tRNA(Leu) has a 249-nucleotide group I intron inserted between the wobble and second bases of the anticodon; the intron self-splices during transcription in vitro [Xu, M. Q., Kathe, S. D., Goodrich-Blair, H., Nierzwicki-Bauer, S. A., & Shub, D. A. (1990) Science 250, 15...

journal_title：Biochemistry

authors： Zaug AJ,McEvoy MM,Cech TR

更新日期：1993-08-10 00:00:00

abstract：:The removal of cylclobutane pyrimidine dimers from cellular DNA occurs preferentially in actively transcribed genes of cells subjected to ultraviolet radiation. In contrast, reports concerning the transcription-dependent repair of N-methylpurines formed in cellular DNA following exposure to methylating agents are quit...

journal_title：Biochemistry

authors： Wang W,Sitaram A,Scicchitano DA

更新日期：1995-02-07 00:00:00

abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...

journal_title：Biochemistry

authors： Wolf B,Hanlon S

更新日期：1975-04-22 00:00:00

abstract：:The association of ferredoxin with ferredoxin:NADP+ reductase (both proteins from spinach chloroplasts) was characterized by isothermal titration calorimetry and fluorescence quenching titration. The formation of the complex is mainly driven by a positive entropy change (delta S = 125 +/- 8 J mol-1 K-1). The calorimet...

journal_title：Biochemistry

authors： Jelesarov I,Bosshard HR

更新日期：1994-11-15 00:00:00

abstract：:We have used a site-specific approach to investigate the mutagenic potential of (+)- and (-)-trans-anti-benzo[a]pyrene diol epoxide (BPDE) DNA adducts. Oligodeoxyribonucleotides (5'TCCTCCTG1G2-CCTCTC), modified at the exocyclic amino groups of G1 or G2, were incorporated into a single-stranded shuttle vector and intro...

journal_title：Biochemistry

authors： Moriya M,Spiegel S,Fernandes A,Amin S,Liu T,Geacintov N,Grollman AP

更新日期：1996-12-24 00:00:00

abstract：:Mammalian cGMP- and cAMP-dependent protein kinase show considerable similarity in amino acid sequence, although they specifically bind different cyclic nucleotides. Results of cGMP analogue binding experiments, combined with modeling of the cGMP binding sites by analogy to the structure of the homologous catabolite ge...

journal_title：Biochemistry

authors： Weber IT,Shabb JB,Corbin JD

更新日期：1989-07-11 00:00:00

abstract：:The paramagnetic enhancements in the NMR relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (PDO). These studies show directly that the substrate i...

journal_title：Biochemistry

authors： Tierney DL,Gassner GT,Luchinat C,Bertini I,Ballou DP,Penner-Hahn JE

更新日期：1999-08-24 00:00:00

abstract：:The human transferrin receptor (TfR) has three N-linked oligosaccharides. A combination of site-directed mutagenesis and carbohydrate and protein chemistry was used to characterize the structures of the N-linked oligosaccharides and to map their locations. We find that the type of oligosaccharide at each position was ...

journal_title：Biochemistry

authors： Hayes GR,Williams AM,Lucas JJ,Enns CA

更新日期：1997-04-29 00:00:00

abstract：:Factor X (FX) is one of the major players in the blood coagulation cascade. Upon activation to FXa, it converts prothrombin to thrombin, which in turn converts fibrinogen into fibrin (blood clots). FXa deficiency causes hemostasis defects, such as intracranial bleeding, hemathrosis, and gastrointestinal blood loss. He...

journal_title：Biochemistry

authors： Abdel-Azeim S,Oliva R,Chermak E,De Cristofaro R,Cavallo L

更新日期：2014-11-11 00:00:00

abstract：:Steady-state and femtosecond time-resolved optical methods have been used to compare the spectroscopic features and energy transfer dynamics of two systematically different light-harvesting complexes from the dinoflagellate Amphidinium carterae: main-form (MFPCP) and high-salt (HSPCP) peridinin-chlorophyll a-proteins....

journal_title：Biochemistry

authors： Ilagan RP,Koscielecki JF,Hiller RG,Sharples FP,Gibson GN,Birge RR,Frank HA

更新日期：2006-11-28 00:00:00

abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...

journal_title：Biochemistry

authors： Tiepold M,Kliche W,Pfannstiel J,Faulstich H

更新日期：2000-02-15 00:00:00

abstract：:Protein binding specificities can be manipulated by redesigning contacts that already exist at an interface or by expanding the interface to allow interactions with residues adjacent to the original binding site. Previously, we developed a strategy, called AnchorDesign, for expanding interfaces around linear binding e...

journal_title：Biochemistry

authors： Hussain M,Angus SP,Kuhlman B

更新日期：2018-07-31 00:00:00

abstract：:The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...

journal_title：Biochemistry

authors： Stopar D,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1996-12-03 00:00:00

abstract：:Synaptotagmin I is a synaptic vesicle protein that contains two C(2) domains and acts as a Ca(2+) sensor in neurotransmitter release. The Ca(2+)-binding properties of the synaptotagmin I C(2)A domain have been well characterized, but those of the C(2)B domain are unclear. The C(2)B domain was previously found to pull ...

journal_title：Biochemistry

authors： Ubach J,Lao Y,Fernandez I,Arac D,Südhof TC,Rizo J

更新日期：2001-05-22 00:00:00

abstract：:C4'-oxidized (C4-AP) and C5'-oxidized abasic sites (DOB) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross-links selectively with dA opposite a 3'-adjacent nucleotide, despite the comparable proximity of an opposing dA. A previous report on UvrABC incision of DNA su...

journal_title：Biochemistry

authors： Ghosh S,Greenberg MM

更新日期：2015-10-13 00:00:00

abstract：:Protein misfolding due to missense mutations is a common pathogenic mechanism in cystathionine β-synthase (CBS) deficiency. In our previous studies, we successfully expressed, purified, and characterized nine CBS mutant enzymes containing the following patient mutations: P49L, P78R, A114V, R125Q, E176K, R266K, P422L, ...

journal_title：Biochemistry

authors： Hnízda A,Majtan T,Liu L,Pey AL,Carpenter JF,Kodíček M,Kožich V,Kraus JP

更新日期：2012-06-12 00:00:00

abstract：:The DNA strand cleavage reaction catalyzed by endonuclease III from Escherichia coli (endo III) on the 3'-side of aldehyde abasic sites proceeds by a syn beta-elimination involving abstraction of the 2'-pro-S proton and formation of a trans alpha,beta-unsaturated aldose product; we previously reported the same stereoc...

journal_title：Biochemistry

authors： Mazumder A,Gerlt JA,Absalon MJ,Stubbe J,Cunningham RP,Withka J,Bolton PH

更新日期：1991-01-29 00:00:00

abstract：:Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...

journal_title：Biochemistry

authors： Arnold WR,Baylon JL,Tajkhorshid E,Das A

更新日期：2017-12-26 00:00:00

abstract：:Type B streptogramins, such as virginiamycin S (VS), are cyclic hexadepsipeptides, inhibiting protein synthesis in prokaryotes. L-Thr connects a 3-hydroxypicolinyl residue (3-OH-Pic) to the peptide lactone ring. The fluorescence intensity of 3-OH-Pic is strongly increased by chelation to alkaline earth cations or bind...

journal_title：Biochemistry

authors： Di Giambattista M,Engelborghs Y,Nyssen E,Clays K,Cocito C

更新日期：1991-07-23 00:00:00

abstract：:Recently, it has been shown that the activation of the Drosophila EGF receptor (DER) by its natural ligand Spitz is inhibited by Argos [Schweitzer, R., et al. (1995) Nature 376, 699-702]. Argos and Spitz both have an EGF-like domain which in the case of Argos differs from that of Spitz and other EGF receptor agonists ...

journal_title：Biochemistry

authors： van de Poll ML,van Vugt MJ,Lenferink AE,van Zoelen EJ

更新日期：1997-06-17 00:00:00

abstract：:There is increasing evidence that a class of cell membrane glycolipids, gangliosides, can mediate the fibrillogenesis and toxicity of Alzheimer's disease amyloid-beta peptide (Abeta). Using lipid monolayers and vesicles as model membranes, we measured the insertion of Abeta into 1,2-dipalmitoyl-sn-glycero-3-phosphocho...

journal_title：Biochemistry

authors： Chi EY,Frey SL,Lee KY

更新日期：2007-02-20 00:00:00