Abstract：
:We have used a photochemical method to map covalent binding sites of the carcinogen benzo[a]pyrenediol epoxide (BPDE) within DNA from the transcriptional control region of the chicken adult beta-globin gene. Our preliminary low-resolution mapping has demonstrated that this region contains highly preferred BPDE binding sites [Boles, T.C., & Hogan, M.E. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 5623-5627]. Here, we find that BPDE binding at individual G residues in this region is influenced by nearest-neighbor interactions and also by longer range interactions that may be attributable to sequence-specific variation of DNA secondary structure. Our findings suggest that long poly(dG) sequences should be preferred sites for BPDE action in other genes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Boles TC,Hogan MEdoi
10.1021/bi00358a045subject
Has Abstractpub_date
1986-05-20 00:00:00pages
3039-43issue
10eissn
0006-2960issn
1520-4995journal_volume
25pub_type
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