Abstract：
:The egg jelly that encapsulates amphibian eggs is essential for fertilization, but its molecular composition and roles remain largely unknown. We identified a calcium-dependent lectin from the pentraxin superfamily in the egg jelly coat from the South American burrowing frog, Lepidobatrachus laevis. This lectin, jeltraxin, was related to the host-response acute phase serum proteins C-reactive P component (CRP) and serum amyloid P component (SAP). The amino acid sequence of jeltraxin is 44% identical to that of Xenopus laevis CRP, 31-35% identical to those of mammalian CRP and SAP, and 21-27% identical to those of the large fusion pentraxins. Expression of jeltraxin mRNA was restricted to the oviduct, which distinguishes it as the first serum-related pentraxin not expressed in the liver. Purified jeltraxin was previously shown to exist in an oligomeric complex of approximately 250 kDa comprised of self-associating subunits. We have demonstrated by MALDI-TOF that this configuration is due to a decameric complex of 27.7 kDa subunits. Biotinylated jeltraxin bound to the high-molecular mass components of the egg jelly in a calcium-dependent manner with specificity for beta-galactose residues. On the basis of homology modeling, we predict that jeltraxin will coordinate two calcium ions. The function of jeltraxin will likely be related to its calcium-dependent lectin properties.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Peavy TR,Hernandez C,Carroll EJ Jrdoi
10.1021/bi035314osubject
Has Abstractpub_date
2003-11-11 00:00:00pages
12761-9issue
44eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Histone H10 differs substantially from H1 in a 37-residue region of the primary structure that is conserved between the usual H1 histones of fish and mammals. Instead, H10 is homologous to H5 in this region. Histones H10 and H5 might be classified together in a histone category distinct from the usual H1. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00511a035
更新日期：1981-04-14 00:00:00
abstract：:Actin depolymerizing factor (ADF)/cofilin enhances turnover of actin filaments by severing and depolymerizing filaments. A number of proteins functionally interact with ADF/cofilin to modulate the dynamics of actin filaments. Actin-interacting protein 1 (AIP1) has emerged as a conserved WD-repeat protein that specific...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi034600x
更新日期：2003-11-25 00:00:00
abstract：:Methenyltetrahydromethanopterin (methenyl-H(4)MPT(+)) cyclohydrolase (Mch) catalyzes the interconversion of methenyl-H(4)MPT(+) and formyl-H(4)MPT in the one-carbon energy metabolism of methanogenic, methanotrophic, and sulfate-reducing archaea and of methylotrophic bacteria. To understand the catalytic mechanism of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300777k
更新日期：2012-10-23 00:00:00
abstract：:The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101509u
更新日期：2010-12-28 00:00:00
abstract：:We have developed a simple method for reconstituting pure, integral membrane proteins into phospholipid-protein vesicles. The method does not depend on use of detergents or sonication. It has been used successfully with three different types of integral membrane proteins: UDPglucuronosyltransferase (EC 2.4.1.17) from ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00336a040
更新日期：1985-07-16 00:00:00
abstract：:Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a064
更新日期：1989-01-24 00:00:00
abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a043
更新日期：1986-11-18 00:00:00
abstract：:Aminoacyl-tRNA synthetases preserve the fidelity of decoding genetic information by accurately joining amino acids to their cognate transfer RNAs. Here, tRNA discrimination at the level of binding by Escherichia coli histidyl-tRNA synthetase is addressed by filter binding, analytical ultracentrifugation, and iodine fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991182g
更新日期：1999-10-12 00:00:00
abstract：:Femtosecond spectroscopy was performed on CO-liganded (fully reduced and mixed-valence states) and O(2)-liganded quinol oxidase bd from Escherichia coli. Substantial polarization effects, unprecedented for optical studies of heme proteins, were observed in the CO photodissociation spectra, implying interactions betwee...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0158019
更新日期：2002-02-05 00:00:00
abstract：:Conceptually one may envisage that substrate binding sites on the ABC transporter P-gp cycle between high- and low-affinity conformations in response to signals arising from nucleotide hydrolysis to effect active transport. A radioligand binding assay was used to characterize the interaction of [3H]vinblastine with P-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011211z
更新日期：2001-12-25 00:00:00
abstract：:The enzyme kinetics of the reduction of the substrate 6,8-dimethylpterin by chicken and recombinant human dihydrofolate reductases (DHFRs) have been examined over the pH range 5.0-8.0 in the presence of NADPH or (4R)-[2H]NADPH (NADPD). The pH profiles of the catalytic constant (Vmax/[E]o or kcat) showed pH independenc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00011a031
更新日期：1995-03-21 00:00:00
abstract：:The distances between the anticodon loops of fluorescent tRNAPhe bound to the E site and to either the A or the P site of poly(U)-programmed Escherichia coli ribosomes were measured by fluorescence energy transfer. Donor and acceptor molecules were wybutine and proflavin, respectively, both located 3' to the anticodon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00358a002
更新日期：1986-05-20 00:00:00
abstract：:The fidelity of DNA strand transfer reactions catalyzed by human immunodeficiency virus type 1 reverse transcriptase has been studied in vitro. A model system involving two sequential DNA strand transfers was developed to simulate the process of forced copy-choice recombination. A propensity for nucleotide misincorpor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00179a014
更新日期：1994-04-05 00:00:00
abstract：:Pectate lyase C (pelC) was the first protein in which the parallel beta-helix structure was recognized. The unique features of parallel beta-helix-containing proteins-a relatively simple topology and unusual interactions among side chains-make pelC an interesting protein to study with respect to protein folding. In th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001900v
更新日期：2000-12-26 00:00:00
abstract：:Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200217u
更新日期：2011-04-12 00:00:00
abstract：:Histidine decarboxylase (HDC) from Lactobacillus 30a forms a trimer around a central cavity or well. Three active sites are formed around the well at the interface of each of two adjacent molecules. HDC exhibits cooperative kinetics at pH 7.6 and can be described in terms of a two-state, T and R, model. At pH 4.8, pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00018a009
更新日期：1995-05-09 00:00:00
abstract：:EPR studies have revealed that removal of calcium using citric acid from the site in spinach photosystem II which is coupled to the photosynthetic O2-evolving process produces a structural change in the manganese cluster responsible for water oxidation. If done in the dark, this yields a modified S1' oxidation state w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00450a032
更新日期：1989-11-28 00:00:00
abstract：:A positional-scanning combinatorial protocol was employed to optimize the deoxyribose-based cleavage of B-form DNA by Ni(II) x Xaa-Xaa-His metallopeptides. This procedure employed 18 naturally occurring amino acids (excluding Cys and Trp) to generate two libraries in which the first and second positions of the peptide...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982587o
更新日期：1999-02-16 00:00:00
abstract：:A conformational analysis of the valine side chains of ribonuclease T1 (RNase T1) was performed using NMR spectroscopy, in particular homonuclear (1H, 1H and 13C, 13C) and heteronuclear (1H, 15N and 1H, 13C) vicinal spin-spin coupling constants as obtained from E.COSY-type NMR experiments. The coupling constants relat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00184a017
更新日期：1994-05-10 00:00:00
abstract：:The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051550i
更新日期：2005-11-08 00:00:00
abstract：:In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801831r
更新日期：2009-02-03 00:00:00
abstract：:CD23/Fc epsilonRII, the low-affinity receptor for IgE, is a multifunctional protein of importance in blood cell development and the immune system. We have studied the interaction of CD23 with IgE in solution using hydrodynamic methods applied to recombinant fragments of both ligands: sCD23, corresponding to the solubl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961231e
更新日期：1997-02-25 00:00:00
abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401027n
更新日期：2013-10-08 00:00:00
abstract：:Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds....
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi400060e
更新日期：2013-06-04 00:00:00
abstract：:The effects of poly(ethylene glycol) (PEG) on sonicated unilamellar vesicles made of phosphatidylcholine have been examined. Stopped-flow and equilibrium data are presented for vesicle aggregation, vesicle leakage, lipid mixing, and aqueous contents mixing. Vesicle aggregation is detected as a monoexponential increase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00065a024
更新日期：1993-04-13 00:00:00
abstract：:Due to their prevalence and roles in biological systems, single mismatches adjacent to G-U pairs are important RNA structural elements. Since there are only limited experimental values for the stability of single mismatches adjacent to G-U pairs, current algorithms using free energy minimization to predict RNA seconda...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800471z
更新日期：2008-09-23 00:00:00
abstract：:alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025752f
更新日期：2002-07-23 00:00:00
abstract：:Band 3, the anion transport protein of human erythrocyte membranes, can be transferred from cells to liposomes and from liposomes back to cell membranes, retaining function and native orientation. After incubation with cells, sonicated phosphatidylcholine vesicles bind a transmembrane protein that comigrates with band...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00295a011
更新日期：1983-12-20 00:00:00
abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi953025x
更新日期：1996-05-21 00:00:00
abstract：:Phoborhodopsin from Halobacterium salinarum (salinarum phoborhodopsin, spR also called HsSR II) is a photoreceptor for the negative phototaxis of the bacterium. A unique feature of spR is the formation of a shorter wavelength photoproduct, P480, observed at liquid nitrogen temperature beside the K intermediate. Format...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200598r
更新日期：2011-08-23 00:00:00
