Jeltraxin, a frog egg jelly glycoprotein, has calcium-dependent lectin properties and is related to human serum pentraxins CRP and SAP.


:The egg jelly that encapsulates amphibian eggs is essential for fertilization, but its molecular composition and roles remain largely unknown. We identified a calcium-dependent lectin from the pentraxin superfamily in the egg jelly coat from the South American burrowing frog, Lepidobatrachus laevis. This lectin, jeltraxin, was related to the host-response acute phase serum proteins C-reactive P component (CRP) and serum amyloid P component (SAP). The amino acid sequence of jeltraxin is 44% identical to that of Xenopus laevis CRP, 31-35% identical to those of mammalian CRP and SAP, and 21-27% identical to those of the large fusion pentraxins. Expression of jeltraxin mRNA was restricted to the oviduct, which distinguishes it as the first serum-related pentraxin not expressed in the liver. Purified jeltraxin was previously shown to exist in an oligomeric complex of approximately 250 kDa comprised of self-associating subunits. We have demonstrated by MALDI-TOF that this configuration is due to a decameric complex of 27.7 kDa subunits. Biotinylated jeltraxin bound to the high-molecular mass components of the egg jelly in a calcium-dependent manner with specificity for beta-galactose residues. On the basis of homology modeling, we predict that jeltraxin will coordinate two calcium ions. The function of jeltraxin will likely be related to its calcium-dependent lectin properties.






Peavy TR,Hernandez C,Carroll EJ Jr




Has Abstract


2003-11-11 00:00:00












  • Mutational analysis reveals a single binding interface between RhoA and its effector, PRK1.

    abstract::Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The st...


    pub_type: 杂志文章


    authors: Hutchinson CL,Lowe PN,McLaughlin SH,Mott HR,Owen D

    更新日期:2011-04-12 00:00:00

  • D18G transthyretin is monomeric, aggregation prone, and not detectable in plasma and cerebrospinal fluid: a prescription for central nervous system amyloidosis?

    abstract::Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...


    pub_type: 杂志文章


    authors: Hammarström P,Sekijima Y,White JT,Wiseman RL,Lim A,Costello CE,Altland K,Garzuly F,Budka H,Kelly JW

    更新日期:2003-06-10 00:00:00

  • Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis.

    abstract::Caveolae are approximately 50-100 nm invaginations of the plasma membrane thought to form as a result of a local accumulation of cholesterol, sphingolipids, and a unique family of three proteins known as the caveolins: Cav-1, -2, and -3. Here, we report the identification, sequence, and developmental expression of the...


    pub_type: 杂志文章


    authors: Razani B,Park DS,Miyanaga Y,Ghatpande A,Cohen J,Wang XB,Scherer PE,Evans T,Lisanti MP

    更新日期:2002-06-25 00:00:00

  • Specificity of lipid incorporation is determined by sequences in the N-terminal 37 of apoB.

    abstract::The N-terminal 17% of apolipoprotein B (apoB-17) is secreted lipid-poor while apoB-41 particles are secreted with a triacylglycerol (TAG)-rich core. Thus, the sequence between apoB-17 and apoB-41 is necessary for the assembly of TAG-rich lipoproteins. To delineate this region, C127 cells were permanently transfected t...


    pub_type: 杂志文章


    authors: Carraway M,Herscovitz H,Zannis V,Small DM

    更新日期:2000-08-15 00:00:00

  • Kinetic and mechanistic studies of the NO*-mediated oxidation of oxymyoglobin and oxyhemoglobin.

    abstract::The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...


    pub_type: 杂志文章


    authors: Herold S,Exner M,Nauser T

    更新日期:2001-03-20 00:00:00

  • Conformational changes in human hepatitis C virus NS3 protease upon binding of product-based inhibitors.

    abstract::One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...


    pub_type: 杂志文章


    authors: Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

    更新日期:1999-10-19 00:00:00

  • Kinetics and equilibria in ligand binding by nitrophorins 1-4: evidence for stabilization of a nitric oxide-ferriheme complex through a ligand-induced conformational trap.

    abstract::Nitrophorins 1-4 (NP1-4) are ferriheme proteins from the blood-sucking insect Rhodnius prolixus that transport nitric oxide (NO) to the victim, sequester histamine, and inhibit blood coagulation. Here, we report kinetic and thermodynamic analyses for ligand binding by all four proteins and their reduction potentials. ...


    pub_type: 杂志文章


    authors: Andersen JF,Ding XD,Balfour C,Shokhireva TK,Champagne DE,Walker FA,Montfort WR

    更新日期:2000-08-22 00:00:00

  • The nuclear transport machinery recognizes nucleoplasmin-histone complexes.

    abstract::The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...


    pub_type: 杂志文章


    authors: Arregi I,Falces J,Bañuelos S,Urbaneja MA,Taneva SG

    更新日期:2011-08-23 00:00:00

  • Single-molecule visualization of environment-sensitive fluorophores inserted into cell membranes by staphylococcal gamma-hemolysin.

    abstract::Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...


    pub_type: 杂志文章


    authors: Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

    更新日期:2006-02-28 00:00:00

  • A hydrophobic inhibitor of the nicotinic acetylcholine receptor acts on the resting state.

    abstract::3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...


    pub_type: 杂志文章


    authors: Wu G,Raines DE,Miller KW

    更新日期:1994-12-27 00:00:00

  • Procatalytic ligand strain. Ionization and perturbation of 8-nitroxanthine at the urate oxidase active site.

    abstract::The binding of the inhibitor 8-nitroxanthine to urate oxidase has been investigated by Raman and UV-visible absorption spectroscopy. The absorption maximum of 8-nitroxanthine shifts from 380 to 400 nm upon binding to the enzyme, demonstrating that the electronic structure of the ligand is perturbed. It has been propos...


    pub_type: 杂志文章


    authors: Doll C,Bell AF,Power N,Tonge PJ,Tipton PA

    更新日期:2005-08-30 00:00:00

  • Two clusters of charged residues located in the electropositive face of the von Willebrand factor A1 domain are essential for heparin binding.

    abstract::The VWF A1 domain seems to possess two heparin binding regions (residues 565-587 and 633-648) displaying positively charged amino acids, but the overall polyanion-A1 domain interaction scheme remains essentially elusive. To probe this molecular reaction as well as the role of electrostatic forces in VWF-heparin intera...


    pub_type: 杂志文章


    authors: Rastegar-Lari G,Villoutreix BO,Ribba AS,Legendre P,Meyer D,Baruch D

    更新日期:2002-05-28 00:00:00

  • Structure, spectroscopic, and redox properties of Rhodobacter sphaeroides reaction centers bearing point mutations near the primary electron donor.

    abstract::Single mutations of three amino acid residues in the vicinity of the primary electron donor, P, in the reaction center (RC) from Rhodobacter (Rb.) sphaeroides were constructed and characterized in order to study the effects of hydrogen-bonding on the physical properties of P. The mutations, Phe M197-->Tyr, Met L248-->...


    pub_type: 杂志文章


    authors: Wachtveitl J,Farchaus JW,Das R,Lutz M,Robert B,Mattioli TA

    更新日期:1993-11-30 00:00:00

  • Biochemical characterization of the roles of glycines 24 and 27 and threonine 179 in tropomyosin from the fast skeletal trunk muscle of the atlantic salmon.

    abstract::Atlantic salmon fast skeletal muscle is composed of an α-type tropomyosin that shares 20 substitutions with a mammalian homologue. Prominent isomorphisms considered to be potentially destabilizing include threonine 179 (core, Ala in rabbit) and a unique pair of glycines, residues 24 and 27. Bacterially expressed mutan...


    pub_type: 杂志文章


    authors: Fudge KR,Heeley DH

    更新日期:2015-05-05 00:00:00

  • Intrinsic fluorescence reports a global conformational change in the N-lobe of human serum transferrin following iron release.

    abstract::Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...


    pub_type: 杂志文章


    authors: James NG,Berger CL,Byrne SL,Smith VC,MacGillivray RT,Mason AB

    更新日期:2007-09-18 00:00:00

  • Hydrophobic core of the steroidogenic acute regulatory protein for cholesterol transport.

    abstract::The steroidogenic acute regulatory protein (StAR), the first family member of START (StAR-related lipid transport) proteins, plays an essential role by facilitating the movement of cholesterol from the outer to inner mitochondrial membrane. Wild-type and mutant StAR binds cholesterol with similar intensity, but only w...


    pub_type: 杂志文章


    authors: Bose HS,Whittal RM,Bose M,Debnath D

    更新日期:2009-02-17 00:00:00

  • Engineering of papain: selective alteration of substrate specificity by site-directed mutagenesis.

    abstract::The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...


    pub_type: 杂志文章


    authors: Khouri HE,Vernet T,Ménard R,Parlati F,Laflamme P,Tessier DC,Gour-Salin B,Thomas DY,Storer AC

    更新日期:1991-09-17 00:00:00

  • Stable DNA-protein cross-links are products of DNA charge transport in a nucleosome core particle.

    abstract::DNA-protein cross-links (DPCs) in nucleosome core particles (NCPs), the fundamental building block of chromatin, arise during times of cellular oxidative stress. These lesions are expected to be detrimental to the cell due to interference with processes like chromatin remodeling, transcription, DNA replication, and ep...


    pub_type: 杂志文章


    authors: Bjorklund CC,Davis WB

    更新日期:2007-09-25 00:00:00

  • Distribution of cytokinin-active nucleosides in isoaccepting transfer ribonucleic acids from Agrobacterium tumefaciens.

    abstract::The cytokinin-active isoprenoid nucleosides of Agrobacterium tumefaciens transfer ribonucleic acid were identified by high-pressure liquid chromatography, permethylation, and mass spectroscopy. Besides the expected 6-[(3-methylbut-2-enyl)amino]-9-(beta-D-ribofuranosyl)purine (i6A) and its 2-methylthio derivative (ms2i...


    pub_type: 杂志文章


    authors: Morris RO,Regier DA,Olson RM Jr,Struxness LA,Armstrong DJ

    更新日期:1981-10-13 00:00:00

  • Thermodynamic characterization of the binding interaction between the histone demethylase LSD1/KDM1 and CoREST.

    abstract::Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...


    pub_type: 杂志文章


    authors: Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

    更新日期:2011-02-01 00:00:00

  • Accumulation of a globo-series glycolipid having Gal alpha 1-3Gal in PC12h pheochromocytoma cells.

    abstract::In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52-58]. Recently, we found that subcloned PC12h cells accumulated another un...


    pub_type: 杂志文章


    authors: Ariga T,Yu RK,Scarsdale JN,Suzuki M,Kuroda Y,Kitagawa H,Miyatake T

    更新日期:1988-07-12 00:00:00

  • Two-dimensional 1H NMR studies of cytochrome c.

    abstract::Two-dimensional nuclear magnetic resonance techniques were used to assign the NH, C alpha H, and C beta H protons of over 60 of the 104 amino acid residues in the 1H NMR spectrum of horse ferrocytochrome c. The majority of these amino acids were completely assigned. Assignments were based on the analysis of two-dimens...


    pub_type: 杂志文章


    authors: Wand AJ,Englander SW

    更新日期:1985-09-24 00:00:00

  • Acylation of lysine 860 allows tight binding and cytotoxicity of Bordetella adenylate cyclase on CD11b-expressing cells.

    abstract::The Bordetella adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) forms cation-selective membrane channels and delivers into the cytosol of target cells an adenylate cyclase domain (AC) that catalyzes uncontrolled conversion of cellular ATP to cAMP. Both toxin activities were previously shown to depend on post-t...


    pub_type: 杂志文章


    authors: Masin J,Basler M,Knapp O,El-Azami-El-Idrissi M,Maier E,Konopasek I,Benz R,Leclerc C,Sebo P

    更新日期:2005-09-27 00:00:00

  • Crystal structure and properties of CYP231A2 from the thermoacidophilic archaeon Picrophilus torridus.

    abstract::The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...


    pub_type: 杂志文章


    authors: Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

    更新日期:2008-02-19 00:00:00

  • Studies with recombinant Saccharomyces cerevisiae CaaX prenyl protease Rce1p.

    abstract::Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...


    pub_type: 杂志文章


    authors: Dolence JM,Steward LE,Dolence EK,Wong DH,Poulter CD

    更新日期:2000-04-11 00:00:00

  • Conformational modulation of troponin T by configuration of the NH2-terminal variable region and functional effects.

    abstract::Troponin T (TnT) is an essential element in the thin filament-based regulatory system of striated muscle. Alternative mRNA splicing generates multiple TnT isoforms with primary structural differences in the NH2-terminal region. The functional significance of this hypervariable NH2-terminal domain and the developmental...


    pub_type: 杂志文章


    authors: Wang J,Jin JP

    更新日期:1998-10-13 00:00:00

  • Comparison of the nerve growth factor proteins from cobra venom (Naja naja) and mouse submaxillary gland.

    abstract::The nerve growth factors (NGF's) isolated from cobra venom (Naja naja) and mouse submaxillary gland are closely related proteins. They are structurally similar in that about 60% of their amino acid residues are identical (Hogue-Angeletti, R. A., Frazier, W. A., Jacobs, J. W., Niall, H.D., and Bradshaw, R. A. (1976), B...


    pub_type: 杂志文章


    authors: Server AC,Herrup K,Shooter EM,Hogue-Angeletti RA,Frazier WA,Bradshaw RA

    更新日期:1976-01-13 00:00:00

  • 19F NMR studies of the D-galactose chemosensory receptor. 2. Ca(II) binding yields a local structural change.

    abstract::The Escherichia coli D-galactose and D-glucose receptor possesses a Ca(II)-binding site closely related in structure and metal-binding characteristics to the eukaryotic EF-hand sites. Only the structure of the Ca(II)-occupied site is known. To investigate the structural change triggered by Ca(II) and Sr(II) binding, w...


    pub_type: 杂志文章


    authors: Luck LA,Falke JJ

    更新日期:1991-04-30 00:00:00

  • Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin.

    abstract::The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...


    pub_type: 杂志文章


    authors: Betlach MC,Kealey JT,Ashley GW,McDaniel R

    更新日期:1998-10-20 00:00:00

  • Selective inactivation of parvulin-like peptidyl-prolyl cis/trans isomerases by juglone.

    abstract::In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...


    pub_type: 杂志文章


    authors: Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

    更新日期:1998-04-28 00:00:00