Allosteric regulation of inducer and operator binding to the lactose repressor.

abstract：:Nuclear magnetic resonance (NMR) has been used to monitor the conformation and dynamics of the d(C1-G2-A3-T4-T5-A6-T6-A5-A4-T3-C2-G1) self-complementary dodecanucleotide duplex (henceforth called Pribnow 12-mer), which contains a TATAAT Pribnow box and a central core of eight dA X dT base pairs. The exchangeable imino...

journal_title：Biochemistry

authors： Patel DJ,Kozlowski SA,Weiss M,Bhatt R

更新日期：1985-02-12 00:00:00

abstract：:Human telomeric DNA consists of tandem repeats of the DNA sequence d(GGGTTA). Oligodeoxynucleotide telomere models such as d[A(GGGTTA)(3)GGG] (Tel22) fold in a cation-dependent manner into quadruplex structures consisting of stacked G-quartets linked by d(TTA) loops. NMR has shown that in Na(+) solutions Tel22 forms a...

journal_title：Biochemistry

authors： Gray RD,Petraccone L,Trent JO,Chaires JB

更新日期：2010-01-12 00:00:00

abstract：:Bacterial L-asparaginases, enzymes that catalyze the hydrolysis of L-asparagine to aspartic acid, have been used for over 30 years as therapeutic agents in the treatment of acute childhood lymphoblastic leukemia. Other substrates of asparaginases include L-glutamine, D-asparagine, and succinic acid monoamide. In this ...

journal_title：Biochemistry

authors： Aghaiypour K,Wlodawer A,Lubkowski J

更新日期：2001-05-15 00:00:00

abstract：:The flexibility and curvature of duplex DNAs containing mismatched sites have been monitored as a function of temperature. The diffusion coefficients are dependent on the flexibility and the curvature of the DNA, and these have been determined by NMR-based methods. The diffusion coefficients, D, depend on a Boltzmann ...

journal_title：Biochemistry

authors： Marathias VM,Jerkovic B,Arthanari H,Bolton PH

更新日期：2000-01-11 00:00:00

abstract：:Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...

journal_title：Biochemistry

authors： Zhang R,Hua G,Andacht TM,Adang MJ

更新日期：2008-10-28 00:00:00

abstract：:Tafazzin is the mitochondrial enzyme that catalyzes transacylation between a phospholipid and a lysophospholipid in remodeling. Mutations in tafazzin cause Barth syndrome, a potentially life-threatening disease with the major symptom being cardiomyopathy. In the tafazzin-deficient heart, cardiolipin (CL) acyl chains b...

journal_title：Biochemistry

authors： Kimura T,Kimura AK,Ren M,Berno B,Xu Y,Schlame M,Epand RM

更新日期：2018-04-10 00:00:00

abstract：:By means of 2D NMR techniques, all backbone resonances in the 1H NMR spectrum of the single-stranded DNA binding protein encoded by gene V of the filamentous phage IKe have been assigned sequence specifically (at pH 4.6, T = 298 K). In addition, a major part of the side chain resonances could be assigned as well. Anal...

journal_title：Biochemistry

authors： van Duynhoven JP,Folkers PJ,Prinse CW,Harmsen BJ,Konings RN,Hilbers CW

更新日期：1992-02-04 00:00:00

abstract：:The bond cleavage step of pepsinogen activation has been investigated in a kinetic study in which the denatured products of short-term acidifications were separated on SDS-polyacrylamide gels and the peptide products were quantitated by densitometry. Although several peptide products were observed, under the condition...

journal_title：Biochemistry

authors： Glick DM,Shalitin Y,Hilt CR

更新日期：1989-03-21 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (GAP) into d-glycerate 1,3-bisphosphate (1,3-diPG) in the presence of NAD(+) and inorganic phosphate (P(i)). Within the active site, two anion-binding sites were ascribed to the binding o...

journal_title：Biochemistry

authors： Castilho MS,Pavão F,Oliva G,Ladame S,Willson M,Périé J

更新日期：2003-06-17 00:00:00

abstract：:Class II tRNA synthetases have long been known to have quaternary structures of alpha, alpha2, alpha2beta2, and alpha4, depending on the amino acid specificity and the organism from which the synthetase was isolated. Even the quaternary structures of enzymes for the same amino acid show variations in evolution. The ba...

journal_title：Biochemistry

authors： Ribas de Pouplana L,Schimmel P

更新日期：1997-12-09 00:00:00

abstract：:In order to understand translocation in transcription, it is important to develop a continuous functional assay for RNA polymerase (RNAP) activity in vitro. Fluorescent derivatives of ATP, UTP, UpA, and CpA with aminonaphthalene-5-sulfonic acid (AmNS) attached to the nucleotide triphosphates via a gamma-phosphoramidat...

journal_title：Biochemistry

authors： Tyagi SC,Wu FY

更新日期：1992-07-21 00:00:00

abstract：:Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...

journal_title：Biochemistry

authors： Lee RT,Lin P,Lee YC

更新日期：1984-08-28 00:00:00

abstract：:A recirculating Langendorff heart preparation is used to characterize the endogenous antithrombin associated reversibly with murine vascular endothelium. Rat hearts are perfused clear of blood and then recirculated with a physiological salt solution. Addition of heparin educes antithrombin activity continuously into t...

journal_title：Biochemistry

authors： Felsch JS,Owen WG

更新日期：1994-01-25 00:00:00

abstract：:To gain insight into the mechanism by which Arg-163 influences oligomerization of alphaA-crystallin, we prepared a series of truncated alphaA-crystallins with or without mutation of the Arg-163 residue. Expression of the proteins was achieved in Escherichia coli BL21 (DE3) pLysS cells, and alphaA-crystallin was purifi...

journal_title：Biochemistry

authors： Rajan S,Chandrashekar R,Aziz A,Abraham EC

更新日期：2006-12-26 00:00:00

abstract：:Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b differ markedly from their mammalian counterparts. Pea H2b has a molecular...

journal_title：Biochemistry

authors： Spiker S,Isenberg I

更新日期：1977-05-03 00:00:00

abstract：:Type I repeat sequences are evolutionarily conserved sequence elements found in the replication origin and transcriptional promoter region of the rRNA genes (rDNA) in Tetrahymena thermophila. An abundant single-stranded DNA binding protein, ssA-TIBF, specifically interacts with the A-rich strand of the Type I repeat s...

journal_title：Biochemistry

authors： Hou Z,Umthun AR,Dobbs DL

更新日期：1995-04-11 00:00:00

abstract：:Remodeling of photosynthetic machinery induced by growing spinach plants under low light intensities reveals an up-regulation of light-harvesting complexes and down-regulation of photosystem II and cytochrome b6f complexes in intact thylakoids and isolated grana membranes. The antenna size of PSII increased by 40-60% ...

journal_title：Biochemistry

authors： Kirchhoff H,Haase W,Wegner S,Danielsson R,Ackermann R,Albertsson PA

更新日期：2007-10-02 00:00:00

abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

journal_title：Biochemistry

authors： Loret EP,Mansuelle P,Rochat H,Granier C

更新日期：1990-02-13 00:00:00

abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...

journal_title：Biochemistry

authors： Kawashima E,Gadek T,Caruthers MH

更新日期：1977-09-20 00:00:00

abstract：:By using some mutant peptides of transcription factor Spl, phosphate backbone contacts with the DNA binding protein containing three zinc fingers have been investigated by alkylation interference, circular permutation, DNase I footprinting, and methylation protection methods. The ethylation interference analyses of Sp...

journal_title：Biochemistry

authors： Nagaoka M,Sugiura Y

更新日期：1996-07-02 00:00:00

abstract：:Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...

journal_title：Biochemistry

authors： Grzyska PK,Ryle MJ,Monterosso GR,Liu J,Ballou DP,Hausinger RP

更新日期：2005-03-15 00:00:00

abstract：:The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...

journal_title：Biochemistry

authors： Ishimori K,Tsuneshige A,Imai K,Morishima I

更新日期：1989-10-17 00:00:00

abstract：:Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

journal_title：Biochemistry

authors： Mizzer JP,Thorpe C

更新日期：1981-08-18 00:00:00

abstract：:Electron spin polarized electron paramagentic resonance (ESP EPR) spectra were obtained with deuterated iron-removed photosynthetic bacterial reaction centers (RCs) to specifically investigate the effect of the rate of primary charge separation, metal-site occupancy, and H-subunit content on the observed P865+QA- char...

journal_title：Biochemistry

authors： Utschig LM,Greenfield SR,Tang J,Laible PD,Thurnauer MC

更新日期：1997-07-15 00:00:00

abstract：:The ability of DNA topoisomerase I to mediate the formation of structurally modified DNA duplexes was studied utilizing suicide substrates containing high-efficiency cleavage sites and acceptor oligonucleotides in which the 5'-terminal nucleotides were varied. When the substrates were nicked duplexes, the divalent cat...

journal_title：Biochemistry

authors： Wang X,Henningfeld KA,Hecht SM

更新日期：1998-02-24 00:00:00

abstract：:Following platelet activation, platelets undergo a dramatic shape change mediated by the actin cytoskeleton and accompanied by secretion of granule contents. While the actin cytoskeleton is thought to influence platelet granule secretion, the mechanism for this putative regulation is not known. We found that disruptio...

journal_title：Biochemistry

authors： Woronowicz K,Dilks JR,Rozenvayn N,Dowal L,Blair PS,Peters CG,Woronowicz L,Flaumenhaft R

更新日期：2010-06-01 00:00:00

abstract：:Arginine vasopressin (AVP) is a nonapeptide long known as an endocrine and paracrine regulator of important systemic functions, namely, vasoconstriction, gluconeogenesis, corticosteroidogenesis, and excretion of water and urea. Here we report, for the first time, that AVP specifically inhibits expression of the cyclin...

journal_title：Biochemistry

authors： Schwindt TT,Forti FL,Juliano MA,Juliano L,Armelin HA

更新日期：2003-02-25 00:00:00

abstract：:The role of pyridoxal 5'-phosphate (PLP) in the radical-mediated amino group migration catalyzed by lysine 2,3-aminomutase from Clostridia SB4 has been investigated by electron spin echo envelope modulation (ESEEM) spectroscopy. This pulsed electron paramagnetic resonance (EPR) method was used to estimate the distance...

journal_title：Biochemistry

authors： Ballinger MD,Frey PA,Reed GH,LoBrutto R

更新日期：1995-08-08 00:00:00

abstract：:Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...

journal_title：Biochemistry

authors： Corbin JA,Dirkx RA,Falke JJ

更新日期：2004-12-28 00:00:00

abstract：:Organophosphate flame retardants are used to inhibit combustion and increase plasticity in plastics and durable foams. While not neurotoxic, these compounds are potential carcinogens, endocrine disrupters, and developmental toxins. The phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) is unique among phosphotriest...

journal_title：Biochemistry

authors： Bigley AN,Narindoshvili T,Xiang DF,Raushel FM

更新日期：2020-03-31 00:00:00