Abstract:
:The ability of DNA topoisomerase I to mediate the formation of structurally modified DNA duplexes was studied utilizing suicide substrates containing high-efficiency cleavage sites and acceptor oligonucleotides in which the 5'-terminal nucleotides were varied. When the substrates were nicked duplexes, the divalent cations Mg2+ and Ca2+ were found to facilitate the topoisomerase I-mediated formation of ligation products containing 3-nucleotide deletions on the scissile strand, but to suppress the formation of 1-nucleotide deletions. The presence of a complementary nucleotide at the 5'-end of the acceptor strand was not required for the ligation reaction to proceed, but duplex formation to produce duplexes containing a mismatch proceeded more slowly than formation of the fully complementary duplex. Topoisomerase I-mediated mismatch formation in the ligation reaction was inhibited more readily by camptothecin than the corresponding ligation reaction to form a fully complementary duplex; the extent of inhibition was comparable for all three mismatches studied. In comparison, the topoisomerase I inhibitors nitidine and coralyne exhibited quite different effects on the same ligation reactions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wang X,Henningfeld KA,Hecht SMdoi
10.1021/bi972707csubject
Has Abstractpub_date
1998-02-24 00:00:00pages
2691-700issue
8eissn
0006-2960issn
1520-4995pii
bi972707cjournal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The positions of single amino acids in the interhelical loop regions and the C-terminal tail of bacteriorhodopsin (bR) were investigated by X-ray diffraction using site-directed heavy-atom labeling. Since wild-type bR does not contain any cysteines, appropriate cysteine mutants were produced with a unique sulfhydryl g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971735s
更新日期:1998-07-21 00:00:00
abstract::A full-length cDNA encoding a calcium-dependent protein kinase with a calmodulin-like domain from Arabidopsis thaliana (AK-1 for Arabidopsis kinase-1) has been expressed as a fusion protein (called AK-1-6H) in Escherichia coli and purified to near homogeneity with high specific activity (typically 2000 nmol min-1 mg-1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00174a008
更新日期:1994-03-01 00:00:00
abstract::The mechanism by which terminal guanidino nitrogen substituted analogues of amiloride inhibit Na-Ca exchange in purified cardiac sarcolemmal membrane vesicles has been investigated. These inhibitors block both Nai-dependent Ca2+ uptake and Nao-dependent Ca2+ efflux. Inhibition of Na-Ca exchange monitored in K+ is nonc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00407a023
更新日期:1988-04-05 00:00:00
abstract::A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9520141
更新日期:1996-01-30 00:00:00
abstract::GGAs are a family of vesicle-coating regulatory proteins that function in intracellular protein transport. A GGA molecule contains four domains, each mediating interaction with other proteins in carrying out intracellular transport. The GAT domain of GGAs has been identified as the structural entity that binds membran...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034334n
更新日期:2003-06-03 00:00:00
abstract::The Type I homodimeric photosynthetic reaction center found in anaerobic gram-positive bacteria of the genus Heliobacteriaceae incorporates FA- and FB-like iron-sulfur clusters similar to those found in Photosystem I as terminal electron acceptors. We recently isolated the PshB protein that harbors the iron-sulfur clu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0622165
更新日期:2007-03-06 00:00:00
abstract::Pf1, a class II filamentous virus, has been investigated by ultraviolet resonance Raman (UVRR) spectroscopy with excitation wavelengths of 257, 244, 238, and 229 nm. The 257-nm UVRR spectrum is rich in Raman bands of the packaged single-stranded DNA (ssDNA) genome, despite the low DNA mass (6%) of the virion. Converse...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981965m
更新日期:1999-03-09 00:00:00
abstract::The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linke...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00447a054
更新日期:1989-10-17 00:00:00
abstract::Further to our studies on the Escherichia coli 30S ribosomal subunit, the detailed surface topography of both 50S subunits and 70S ribosomes has been investigated by using iodination catalyzed by immobilized lactoperoxidase as the surface probe. In the 50S subunit, only proteins L2, L5, L10, and L11 were iodinated to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00282a019
更新日期:1983-06-21 00:00:00
abstract::The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00013
更新日期:2015-04-14 00:00:00
abstract::Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a002
更新日期:1976-10-19 00:00:00
abstract::We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971009p
更新日期:1998-02-24 00:00:00
abstract::The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00093a022
更新日期:1993-10-26 00:00:00
abstract::Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00607a034
更新日期:1978-07-11 00:00:00
abstract::Single lamellar phosphatidyl[methyl-2H]choline vesicles were incubated with an excess of unlabeled phosphatidylcholine vesicles or phosphatidylcholine-cholesterol vesicles containing 8 mol % glucuronosyldiglyceride. Incubation of the two vesicle populations was performed in the presence or absence of a purified phosph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00534a017
更新日期:1982-03-02 00:00:00
abstract::The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00338a011
更新日期:1985-08-13 00:00:00
abstract::The phagocyte superoxide-generating NADPH oxidase, a multicomponent, membrane-bound electron transport chain, consists of cytochrome b558, p47-phox, p67-phox, and p21rac1 or p21rac2. The mechanisms of oxidase assembly are poorly understood. In previous studies using a cell-free NADPH oxidase system, we showed that pre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00175a018
更新日期:1994-03-08 00:00:00
abstract::To identify the elements which regulate the liver transcription of the human type II phospholipase A2 gene and its stimulation by interleukin 6, the 5' flanking region from -1614 to +806 and several 3' and 5' deleted fragments have been analyzed in CAT assays. Negative regulatory elements have been located in the regi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00189a017
更新日期:1994-06-14 00:00:00
abstract::The mechanism of N-methyltryptophan oxidase, a flavin-dependent amine oxidase from Escherichia coli, was studied using a combination of kinetic isotope effects and theoretical calculations. The 15(kcat/Km) kinetic isotope effect for sarcosine oxidation is pH-dependent with a limiting value of 0.994-0.995 at high pH. D...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700482h
更新日期:2007-06-26 00:00:00
abstract::The biosynthesis of epothilones, a family of hybrid polyketide (PK)/nonribosomal peptide (NRP) antitumor agents, provides an ideal system to study a hybrid PK/NRP natural product with significant biomedical value. Here the third enzyme involved in epothilone production, the five domain 195 kDa polyketide synthase (PKS...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020006w
更新日期:2002-04-30 00:00:00
abstract::Arginine-70 of human dihydrofolate reductase (hDHFR) is a highly conserved residue which X-ray crystallographic data have shown to interact with the alpha-carboxylate of the terminal L-glutamate moiety of either folic acid or methotrexate (MTX). The rationale for this study was to introduce a conservative amino acid r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00247a005
更新日期:1991-08-20 00:00:00
abstract::During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00824
更新日期:2018-04-03 00:00:00
abstract::The kinetics and mechanism of cleavage of DNA by the insulin-mimetic peroxo-vanadate NH4[VO(O2)2(phen)], pV, are described. In the presence of low energy UV radiation or biologically common reducing agents, pV decomposes into the monomer, dimer, and tetramer of vanadate and an uncharacterized compound of V4+ as shown ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9606253
更新日期:1996-09-24 00:00:00
abstract::The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi6024947
更新日期:2007-05-29 00:00:00
abstract::Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9002265
更新日期:2009-06-09 00:00:00
abstract::The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmeny...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00022a008
更新日期:1995-06-06 00:00:00
abstract::Life span regulation and inhibition of gene silencing in yeast have been linked to nicotinamide effects on Sir2 enzymes. The Sir2 enzymes are NAD(+)-dependent protein deacetylases that influence gene expression by forming deacetylated proteins, nicotinamide and 2'-O-acetyl-ADPR. Nicotinamide is a base-exchange substra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034959l
更新日期:2003-08-12 00:00:00
abstract::Base excision repair is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). Base excision repair requires the coordinated action of several repair and ancillary proteins, the impairment of which can lead to genetic instability. Using a protein-DNA cross-linking a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050085m
更新日期:2005-08-09 00:00:00
abstract::The thermotropic behavior of 20 chemically related glycosphingolipids (GSLs) of high purity, containing neutral and anionic carbohydrate residues in their oligosaccharide chains, was studied by high-sensitivity differential scanning calorimetry. In general, the polar head group of GSLs appears to be one of the major d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00326a003
更新日期:1985-02-26 00:00:00
abstract::In prokaryotes, a large share of the proteins are secreted from the cell through a process that requires their translocation across the cytoplasmic membrane. This process is mediated by the universally conserved Sec system with homologues in the endoplasmic reticulum and thylakoid membranes of eukaryotes. The Sec syst...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi400913x
更新日期:2013-10-01 00:00:00