Abstract：
:The complete amino acid sequence of human liver NADPH-cytochrome P-450 reductase has been determined by microsequence analysis and mass spectrometry. The total sequence consists of 676 amino acids initiated by an amino-terminal acetyl group. There is no evidence for posttranslational modifications, including Asn-linked glycosylation. The human enzyme exhibits sequence homology in the range of 92-95% with other mammalian enzymes. Sequence differences were mainly confined to several hydrophilic regions in the NH2-terminal and COOH-terminal domains. Since the human enzyme is immunochemically distinct from the rabbit enzyme despite similar enzymatic properties, it is likely that these variable hydrophilic regions are potential antigenic determinants. The NADPH-depleted enzyme is inactivated by either fluorescein isothiocyanate, a lysine-specific reagent, or 5-(iodoacetamido)fluorescein, a cysteine-specific reagent. In both cases, protection by NADP(H) prevents enzyme inactivation by the reagents. Isolation of fluorescent peptide from 5-(iodoacetamido)fluorescein-inactivated enzyme identified Cys 565 as the specifically NADPH-protected residue.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Haniu M,McManus ME,Birkett DJ,Lee TD,Shively JEdoi
10.1021/bi00447a054subject
Has Abstractpub_date
1989-10-17 00:00:00pages
8639-45issue
21eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. X-ray crystal structures of arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and urea, complete a set of struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002318+
更新日期：2001-03-06 00:00:00
abstract：:We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951584y
更新日期：1996-03-12 00:00:00
abstract：:Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00555a043
更新日期：1980-07-08 00:00:00
abstract：:The plasma membrane of the Ehrlich ascites tumor cell contains an NADH dehydrogenase. This activity was shown not to be due to contamination by other subcellular membranes. A variety of electron acceptors have been compared as to rate with the following result: ferricyanide greater than cytochrome c greater than cytoc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00575a021
更新日期：1979-04-17 00:00:00
abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049821x
更新日期：2004-05-11 00:00:00
abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00679a018
更新日期：1975-04-22 00:00:00
abstract：:The structural and functional roles of two conserved active site residues, Arg-97 and Arg-132, in chicken muscle adenylate kinase (AK) were evaluated by site-directed mutagenesis in conjunction with one- and two-dimensional proton nuclear magnetic resonance (NMR), kinetics, and guanidine hydrochloride-induced denatura...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00142a022
更新日期：1992-07-14 00:00:00
abstract：:Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0027581
更新日期：2001-03-27 00:00:00
abstract：:Fas and its ligand (FasL) are members of the tumor necrosis factor receptor (TNFR) and tumor necrosis factor (TNF) superfamilies, respectively. Fas-FasL interactions trigger controlled cell death (apoptosis) in the immune system and thus play a key role in the regulation of immune responses. Structural details of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972959d
更新日期：1998-03-17 00:00:00
abstract：:It had been reported previously that the beta2 subunit of Escherichia coli tryptophan synthetase [L-serinehydrolyase (adding indole) EC 4.2.1.20] can be cleaved by trypsin into a nearly functional dimeric protein, the monomer of which consists of two large, nonoverlapping, polypeptide fragments. In the present paper, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00637a012
更新日期：1977-09-06 00:00:00
abstract：:In the field of muscle regulation, there is still controversy as to whether Ca2+, alone, is able to shift muscle from the relaxed to the fully active state or whether cross-bridge binding also contributes to turning on muscle contraction. Our previous studies on the binding of myosin subfragment 1 (S-1) to the troponi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00418a048
更新日期：1988-09-06 00:00:00
abstract：:The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG) is responsible for the removal of uracil in DNA as the first step in the base excision repair (BER) pathway. AFUDG contains a large solvent-exposed peptide region containing an α helix and loop anchored on each end v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3000462
更新日期：2012-06-26 00:00:00
abstract：:We have used diethyl pyrocarbonate (DEP), which carbethoxylates adenine bases, and dimethyl sulfate (DMS), which methylates guanine residues and single-stranded cytosines, to probe bases in open complexes between RNA polymerase and the lac UV5 promoter in vitro. We compared the kinetics of reactivity between bases in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00436a040
更新日期：1989-05-16 00:00:00
abstract：:The kinetics of the tryptophan fluorescence enhancement that occurs when myofibrils (rabbit psoas) are mixed with Mg-ATP were studied by stopped-flow in different solvents (water, 40% ethylene glycol, 20% methanol) at 4 degrees C. Under relaxing conditions (low Ca(2+)) in water (mu = 0.16 M, pH 7.4) and at high ATP co...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0004753
更新日期：2000-06-27 00:00:00
abstract：:Fourier transform infrared (FTIR) spectroscopy and a library of FTIR marker bands have been used to examine the structure and relative flexibilities conferred by different flanking sequences on the EcoRI binding site. This approach allowed us to examine unique peaks and subtle changes in the spectra of d(AAAGAATTCTTT)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8015235
更新日期：2009-02-17 00:00:00
abstract：:Because of its ability to selectively glucosylate misfolded glycoproteins, UDP-glucose:glycoprotein glucosyltransferase (UGGT) functions as a folding sensor in the glycoprotein quality control system in the endoplasmic reticulum (ER). The unique property of UGGT derives from its ability to transfer a glucose residue t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00785
更新日期：2015-08-11 00:00:00
abstract：:Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a024
更新日期：1975-09-23 00:00:00
abstract：:The mechanism of bovine intestinal 5'-nucleotide phosphodiesterase was investigated by determining kinetic constants of systematically varied substrates, with emphasis on esters of phosphonic acids (which have much higer Vmax values than conventional phosphodiester substrates), and by pre-steady-state kinetics using b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00625a011
更新日期：1977-03-22 00:00:00
abstract：:It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0006767
更新日期：2000-09-12 00:00:00
abstract：:The channel-forming protein OmpF porin from Escherichia coli spans the bacterial outer membrane. Each of the three monomers comprises a hollow, 16-stranded beta-barrel. These are associated to homotrimers which are unusually stable, due mostly to hydrophobic interactions between the beta-barrels. In addition, a loop, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981215c
更新日期：1998-11-10 00:00:00
abstract：:We have performed comparative studies of the neutral glycosphingolipids synthesized by three human myeloid leukemia cell lines, K562, KG1, and HL-60, which were metabolically labeled with [14C]galactose, to evaluate changes in neutral glycosphingolipid synthesis with myeloid cell differentiation. Individual neutral gl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00345a034
更新日期：1985-11-19 00:00:00
abstract：:Substitution of Co(II) for the catalytic site Zn(II) of horse liver alcohol dehydrogenase (LADH) yields an active enzyme derivative, CoIIE, with characteristic Co(II) charge-transfer and d-d electronic transitions that are sensitive to the events which take place during catalysis [Koerber, S. C., MacGibbon, A. K. H., ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00377a031
更新日期：1987-02-10 00:00:00
abstract：:Two tetrapeptide sequence homologies between mouse epidermal growth factor precursor (mEGFP) and human follitropin (FSH) were revealed by a computer program that identifies identical residues among polypeptide sequences. The two tetrapeptides, Lys-Thr-Cys-Thr (KTCT) and Thr-Arg-Asp-Leu (TRDL), are present in the hormo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00357a053
更新日期：1986-05-06 00:00:00
abstract：:The direct interrogation of fleeting intermediates by rapid-mixing kinetic methods has significantly advanced our understanding of enzymes that utilize dioxygen. The gas's modest aqueous solubility (<2 mM at 1 atm) presents a technical challenge to this approach, because it limits the rate of formation and extent of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201906x
更新日期：2012-02-28 00:00:00
abstract：:Fluorescein isothiocyanate derivatization of the human lactotransferrin on Lys-264 inhibits the binding of the protein of human PHA-activated lymphocytes [Legrand, D., Mazurier, J., Maes, P., Rochard, E., Montreuil, J., & Spik, G. (1991) Biochem. J. 276, 733-738], indicating that part of the receptor-binding site is l...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00153a018
更新日期：1992-09-29 00:00:00
abstract：:The contributions to thermostability of interactions within the beta-sheet region of rubredoxins (Rds) were investigated by examining proteins in which beta-strand sequences of Rds from the hyperthermophilic archaeon Pyrococcus furiosus (Pf) and the mesophilic bacterium Clostridium pasteurianum (Cp) were interchanged....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970110r
更新日期：1997-08-26 00:00:00
abstract：:Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00384a015
更新日期：1987-05-19 00:00:00
abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400514k
更新日期：2013-07-02 00:00:00
abstract：:Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00084
更新日期：2019-04-23 00:00:00
abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961360v
更新日期：1996-12-10 00:00:00
