Abstract:
:Fluorescein isothiocyanate derivatization of the human lactotransferrin on Lys-264 inhibits the binding of the protein of human PHA-activated lymphocytes [Legrand, D., Mazurier, J., Maes, P., Rochard, E., Montreuil, J., & Spik, G. (1991) Biochem. J. 276, 733-738], indicating that part of the receptor-binding site is located in the N-terminal domain I of lactotransferrin. In the present study, a 6-kDa peptide (residues 4-52) was isolated from the N-terminal lobe of human lactotransferrin which inhibited the binding of the protein to its cell receptor. In addition, lactotransferrin was derivatized using sulfosuccinimidyl 2-(p-azidosalicylamido)ethyl-1,3'-dithiopropionate (SASD) and sulfosuccinimidyl 6-((4'-azido-2'-nitrophenyl)amino)hexanoate (sulfo-SANPAH), two heterobifunctional reagents generally used for receptor-ligand cross-linking. The azide group of these two reagents was inactivated by photolysis, and only the succinimidyl ester group was allowed to react with lysine residues of the protein. The binding of the derivatized lactotransferrins to the human lymphocyte receptor was assayed. SASD, which binds to Lys-74, was able to inhibit the binding of lactotransferrin to the cell receptor, in contrast to Lys-281-binding sulfo-SANPAH. Molecular modeling showed the position of SASD, sulfo-SANPAH, and fluorescein molecules at the surface of the protein and suggested that SASD and fluorescein could mask residues 4-6 and two loop-containing regions of human lactotransferrin (residues 28-34 and 38-45). The comparison of the primary and tertiary structures of human lactotransferrin and serotransferrin, which bind to specific cell receptors, shows that the above-mentioned regions, which are likely involved in protein-receptor interactions, possess specific structural features.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Legrand D,Mazurier J,Elass A,Rochard E,Vergoten G,Maes P,Montreuil J,Spik Gdoi
10.1021/bi00153a018subject
Has Abstractpub_date
1992-09-29 00:00:00pages
9243-51issue
38eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A major challenge to the study of protein folding is the fact that intermediate states along the reaction pathway are generally unstable and thus difficult to observe. Recently developed NMR relaxation dispersion experiments present an avenue to accessing such states, providing kinetic, thermodynamic, and structural i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051771o
更新日期:2005-11-29 00:00:00
abstract::Telomeres terminate nearly exclusively in single-stranded DNA (ssDNA) overhangs comprised of the G-rich 3' end. This overhang varies widely in length from species to species, ranging from just a few bases to several hundred nucleotides. These overhangs are not merely a remnant of DNA replication but rather are the res...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00496
更新日期:2016-09-27 00:00:00
abstract::The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952626l
更新日期:1996-03-19 00:00:00
abstract::Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00067
更新日期:2017-04-11 00:00:00
abstract::The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100055m
更新日期:2010-05-11 00:00:00
abstract::To understand the details of rate limitation of turnover of the photosynthetic reaction center, photooxidation of horse heart cytochrome c by reaction center from Rhodobacter spheroides in detergent dispersion has been examined by intense continuous illumination under a wide variety of conditions of cytochrome concent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991563u
更新日期:1999-12-21 00:00:00
abstract::Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400288p
更新日期:2013-07-30 00:00:00
abstract::We have measured the temperature dependence of the oxygen-binding isotherms of human and bovine hemoglobin at pH 9.0 in 0.1 M borate buffer. In both hemoglobins the ionization of the Bohr protons is finished at this pH; therefore, their heat does not interfere with the measurements. Two sets of curves have been obtain...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00227a006
更新日期:1991-04-02 00:00:00
abstract::The association of nucleolar phosphoprotein C23 with preribosomal ribonucleoprotein (RNP) particles was examined in Novikoff hepatoma nucleoli. RNA was labeled with [3H]uridine for various times in cell suspensions, and RNP particles were extracted from isolated nucleoli and fractionated by sucrose gradient ultracentr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00368a063
更新日期:1986-10-07 00:00:00
abstract::Urease from Klebsiella aerogenes is composed of three subunits (UreA-UreB-UreC) that assemble into a (UreABC)(3) quaternary structure. UreC harbors the dinuclear nickel active site, whereas the functions of UreA and UreB remain unknown. UreD and UreF accessory proteins previously were suggested to reposition UreB and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2011064
更新日期:2011-11-01 00:00:00
abstract::The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00399a022
更新日期:1987-12-15 00:00:00
abstract::ATP and the fluorescent substrate analogue TNP-ATP bind competitively to the gastric H,-K-ATPase. Substrate and product completely reverse the fluorescence enhancement caused by TNP-ATP binding to the enzyme. The fluorophore is displaced monophasically from apoenzyme. However, ATP displaces TNP-ATP from the Mg2+-quenc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00442a034
更新日期:1989-08-08 00:00:00
abstract::To date, rational redesign of glycosidase active-site clefts has been mainly limited to the removal of essential functionalities rather than their introduction. The glycoside hydrolase family 26 endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi depolymerizes various abundant plant mannans. On the basis...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100097f
更新日期:2010-06-15 00:00:00
abstract::Two photoreactive apamin derivatives were prepared with an aryl azide [[(azidonitrophenyl)amino]acetate (ANPAA)] group coupled at different positions on the neurotoxin molecule. These ligands were used to identify membrane components in the environment of the neuronal binding site that is associated with a Ca2+-activa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00362a010
更新日期:1986-07-15 00:00:00
abstract::Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a022
更新日期:1987-06-30 00:00:00
abstract::Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00087a023
更新日期:1993-09-14 00:00:00
abstract::The ileal apical and liver basolateral bile acid transporters catalyze the Na+-dependent uptake of these amphipathic molecules in the intestine and liver. They contain nine predicted helical hydrophobic sequences (H1-H9) between the exoplasmic N-glycosylated N terminus and the cytoplasmic C terminus. Previous in vitro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051291x
更新日期:2005-10-25 00:00:00
abstract::Proton inventories (rate measurements in mixtures of H2O and D2O) were determined for the human leukocyte elastase catalyzed hydrolyses of thiobenzyl esters and p-nitroanilides of the peptides MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The dependencies of k2/Ks on mole fraction of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00379a016
更新日期:1987-03-10 00:00:00
abstract::Vitamin D receptor (VDR) is a member of the nuclear hormone receptor superfamily. When bound to a variety of vitamin D analogues, VDR manifests a wide diversity of physiological actions. The molecular mechanism by which different vitamin D analogues cause specific responses is not understood. The published crystallogr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201637p
更新日期:2011-12-27 00:00:00
abstract::The relationship between GTP hydrolysis and microtubule assembly has been investigated by using a rapid filtration method. Microtubules assembled from phosphocellulose-purified tubulin, double-labeled with [gamma-32P]- and [3H]GTP, were trapped and washed free of unbound nucleotide on glass fiber filters. The transien...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00479a022
更新日期:1990-07-10 00:00:00
abstract::Inhibition of the yeast F(0)F(1)-ATP synthase by the regulatory peptides IF1 and STF1 was studied using intact mitochondria and submitochondrial particles from wild-type cells or from mutants lacking one or both peptides. In intact mitochondria, endogenous IF1 only inhibited uncoupled ATP hydrolysis and endogenous STF...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034394t
更新日期:2003-06-24 00:00:00
abstract::31P NMR measurements were made (at 121.5 MHz and 5 degrees C) on enzyme-bound substrate complexes of 3-phosphoglycerate kinase in order to address three questions pertaining to (i) the integrity of the enzyme-substrate complexes with Mg(II) in the presence of sulfate concentrations typical of those used for crystalliz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00415a027
更新日期:1988-07-26 00:00:00
abstract::Standard preparations of Escherichia coli RNA polymerase harbor a 70 kDa protein with NTPase (beta-gamma cleavage) activity that is not a recognized polymerase subunit. The NTPase activity of this component, before and after separation from the polymerase, is strongly dependent on the presence of DNA; single-stranded ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970191r
更新日期:1997-12-02 00:00:00
abstract::Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0005456
更新日期:2000-08-01 00:00:00
abstract::Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00179a011
更新日期:1994-04-05 00:00:00
abstract::Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00687a028
更新日期:1975-08-12 00:00:00
abstract::1H NMR spectroscopy of the isotropically shifted signals in cobalt carboxypeptidase, CoCPD, permits a direct and selective detection of protons belonging to the residues liganded to the metal. The chemical shift of these protons in the free enzyme and enzyme-inhibitor complexes with changing pH monitors the state of i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00130a015
更新日期:1992-04-21 00:00:00
abstract::The peptide hormone bradykinin exerts important biological functions by binding to and activating bradykinin B2 receptors. B2 receptors belong to the seven transmembrane domain (7TM) receptor family. Cloning of the cDNA sequences for the rat, human, and mouse bradykinin B2 receptor revealed several in-frame AUG triple...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9601060
更新日期:1996-06-11 00:00:00
abstract::Uniform double labeling of proteins for NMR studies can be prohibitively expensive, even with an efficient expression and purification scheme, due largely to the high cost of [13C6, 99%]glucose. We demonstrate here that uniformly (greater than 95%) 13C and 15N double-labeled proteins can be prepared for NMR structure/...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00232a017
更新日期:1991-05-07 00:00:00
abstract::Binding of 125I-labeled human alpha-thrombin to endothelial cells derived from bovine corneas was studied in tissue culture. Specific and saturable binding to the cell surface occurred at 37 degrees C but to a much smaller extent at 4 degrees C. Binding of [125I]thrombin to a specific site on these cells with formatio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00505a027
更新日期:1981-01-20 00:00:00