Analysis of the ligand binding site in Fas (CD95) by site-directed mutagenesis and comparison with TNFR and CD40.

abstract：:Typical cytochrome P450s secure the heme prosthetic group with a cysteine thiolate ligand bound to the iron, electrostatic interactions with the heme propionate carboxylates, and hydrophobic interactions with the heme periphery. In addition to these interactions, CYP4B1 covalently binds heme through a monoester link f...

journal_title：Biochemistry

authors： Baer BR,Schuman JT,Campbell AP,Cheesman MJ,Nakano M,Moguilevsky N,Kunze KL,Rettie AE

更新日期：2005-10-25 00:00:00

abstract：:The bovine prothrombin gene was characterized by Southern blot analysis of bovine genomic DNA using bovine prothrombin cDNA fragments as hybridization probes. These analyses suggested that the bovine genome contains a single prothrombin gene that is at least 10 kilobase pairs (kbp) in size. To characterize the gene mo...

journal_title：Biochemistry

authors： Irwin DM,Ahern KG,Pearson GD,MacGillivray RT

更新日期：1985-11-19 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...

journal_title：Biochemistry

authors： Lu T,Tan H,Lee D,Chen G,Jia Z

更新日期：2009-08-25 00:00:00

abstract：:The proprotein convertase furin is a highly specific serine protease modifying and thereby activating proteins in the secretory pathway by proteolytic cleavage. Its substrates are involved in many diseases, including cancer and infections caused by bacteria and viruses. Understanding furin's substrate specificity is c...

journal_title：Biochemistry

authors： Dahms SO,Hardes K,Steinmetzer T,Than ME

更新日期：2018-02-13 00:00:00

abstract：:The role of the interaction between actin and the secondary actin binding site of myosin (segment 565-579 of rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been studied with proteolytically generated smooth and skeletal muscle myosin subfragment 1 and recombinant Dictyostelium discoideum myosin...

journal_title：Biochemistry

authors： Van Dijk J,Furch M,Lafont C,Manstein DJ,Chaussepied P

更新日期：1999-11-16 00:00:00

abstract：:Total internal reflection fluorescence microscopy (TIRFM) has been used to directly measure the weak dissociation constants of IgG with a mouse IgG receptor (moFc gamma RII) that has been purified and reconstituted into substrate-supported planar membranes. Dissociation constants were measured for three different mous...

journal_title：Biochemistry

authors： Hsieh HV,Poglitsch CL,Thompson NL

更新日期：1992-11-24 00:00:00

abstract：:We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...

journal_title：Biochemistry

authors： Dibrov P,Young PG,Fliegel L

更新日期：1998-06-09 00:00:00

abstract：:FixL is a heme-based oxygen-sensing histidine kinase that induces the expression of nitrogen fixation genes under hypoxic conditions. Oxygen dissociation from heme iron in the sensor domain of FixL initiates protein conformational changes that are transmitted to the histidine kinase domain, activating autophosphorylat...

journal_title：Biochemistry

authors： Yamawaki T,Ishikawa H,Mizuno M,Nakamura H,Shiro Y,Mizutani Y

更新日期：2016-07-26 00:00:00

abstract：:The hairpin ribozyme is an RNA enzyme that performs site-specific phosphodiester bond cleavage between nucleotides A-1 and G+1 within its cognate substrate. Previous functional studies revealed that the minimal hairpin ribozyme exhibited "gain-of-function" cleavage properties resulting from U39C or U39 to propyl linke...

journal_title：Biochemistry

authors： Alam S,Grum-Tokars V,Krucinska J,Kundracik ML,Wedekind JE

更新日期：2005-11-08 00:00:00

abstract：:The insulin hexamer is an allosteric protein widely used in formulations for the treatment of diabetes. The hexamer exhibits positive and negative cooperativity and apparent half-site binding activity, reflecting the interconversion of three allosteric states, designated as T6, T3R3, and R6. The hexamer contains two s...

journal_title：Biochemistry

authors： Bonaccio M,Ghaderi N,Borchardt D,Dunn MF

更新日期：2005-05-31 00:00:00

abstract：:In an attempt to understand the multifunctional involvement of beta(2)-glycoprotein I (beta(2)GPI) in autoimmune diseases, thrombosis, atherosclerosis, and inflammatory processes, substantial interest is focused on the interaction of beta(2)GPI with negatively charged ligands, in particular, with acidic phospholipids....

journal_title：Biochemistry

authors： Hammel M,Schwarzenbacher R,Gries A,Kostner GM,Laggner P,Prassl R

更新日期：2001-11-27 00:00:00

abstract：:Two subfractions of low-density lipoproteins (LDL) were isolated from normal pig (Sus domesticus) serum by a combined method including precipitation, ultracentrifugation, and gel chromatography. The fractions recovered from the buoyant density ranges 1.020-1.050 and 1.050-1.090 g/mL, denoted as LDL1 and LDL2, respecti...

journal_title：Biochemistry

authors： Jürgens G,Knipping GM,Zipper P,Kayushina R,Degovics G,Laggner P

更新日期：1981-05-26 00:00:00

abstract：:Isoform 1 of the mammalian Na(+)/H(+) exchanger (NHE1) is a ubiquitously expressed plasma membrane pH regulatory protein. It removes one intracellular H(+) in exchange for one extracellular Na(+). The 500 N-terminal amino acids comprise the catalytic membrane domain and fold into 12 transmembrane (TM) segments. To gai...

journal_title：Biochemistry

authors： Alves C,Lee BL,Sykes BD,Fliegel L

更新日期：2014-06-10 00:00:00

abstract：:Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the ...

journal_title：Biochemistry

authors： Kim PW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

更新日期：2014-05-06 00:00:00

abstract：:The egg vitelline envelope of the marine invertebrate, Megathura crenulata, was lyzed either by sperm lysins A, B, C or by dithiothreitol. In each case the lysis mixture consisted of two major fractions, I and II, that could be separated by hydroxylapatite chromatography and had different electrophoretic mobilities on...

journal_title：Biochemistry

authors： Heller E,Raftery MA

更新日期：1976-03-23 00:00:00

abstract：:Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8) are cell surface nucleotidases with two transmembranous domains, one each at the N- and C-termini. Hydrolysis of substrates occurs in active sites residing in their extracellular domains. Human ecto-ATPase activity is decreased by NP...

journal_title：Biochemistry

authors： Mukasa T,Lee Y,Knowles AF

更新日期：2005-08-23 00:00:00

abstract：:The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...

journal_title：Biochemistry

authors： Fujioka M,Takata Y

更新日期：1981-02-03 00:00:00

abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...

journal_title：Biochemistry

authors： Schwehm JM,Kristyanne ES,Biggers CC,Stites WE

更新日期：1998-05-12 00:00:00

abstract：:The plasma membrane serotonin transporter (SERT) has an important role in terminating serotonergic neurotransmission by re-uptake of 5-HT from the synaptic cleft. The expression of SERT on the cell surface is therefore a critical factor. In this study, we examined the role of the carboxyl terminus of SERT in trafficki...

journal_title：Biochemistry

authors： Larsen MB,Fjorback AW,Wiborg O

更新日期：2006-01-31 00:00:00

abstract：:Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...

journal_title：Biochemistry

authors： Wang W,Fu G,Luo BH

更新日期：2010-11-30 00:00:00

abstract：:We have investigated the interaction of the bisbenzimidazole derivative Hoechst 33258 with the self-complementary dodecadeoxynucleotide duplex d(GTGGAATTCCAC)2 using one-dimensional (1D) and two-dimensional (2D) proton nuclear magnetic resonance (1H NMR) spectroscopy. To monitor the extent of complex formation, we use...

journal_title：Biochemistry

authors： Fede A,Labhardt A,Bannwarth W,Leupin W

更新日期：1991-12-03 00:00:00

abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...

journal_title：Biochemistry

authors： Quantin B,Murphy G,Breathnach R

更新日期：1989-06-27 00:00:00

abstract：:PLK1 (polo-like kinase 1) is a key mitotic kinase and a therapeutic target in the treatment of proliferative diseases. Here we investigate the relative substrate specificity and pharmacological relatedness of PLK1, -2, -3, and -4 that together comprise a conserved family of Ser/Thr kinases (PLK family). We report cons...

journal_title：Biochemistry

authors： Johnson EF,Stewart KD,Woods KW,Giranda VL,Luo Y

更新日期：2007-08-21 00:00:00

abstract：:A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potass...

journal_title：Biochemistry

authors： Benveniste K,Wilczek J,Ruggieri A,Stern R

更新日期：1976-02-24 00:00:00

abstract：:The plasma membrane of the cell is a complex, tightly regulated, heterogeneous environment shaped by proteins, lipids, and small molecules. Ca2+ ions are important cellular messengers, spatially separated from anionic lipids. After cell injury, disease, or apoptotic events, anionic lipids are externalized to the outer...

journal_title：Biochemistry

authors： Hallock MJ,Greenwood AI,Wang Y,Morrissey JH,Tajkhorshid E,Rienstra CM,Pogorelov TV

更新日期：2018-12-18 00:00:00

abstract：:Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...

journal_title：Biochemistry

authors： Zhu J,Lippa GM,Gulick AM,Tipton PA

更新日期：2015-04-28 00:00:00

abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...

journal_title：Biochemistry

authors： Króliczewski J,Hombek-Urban K,Szczepaniak A

更新日期：2005-05-24 00:00:00

abstract：:Cysteine desulfurases (CDs) are pyridoxal-5'-phosphate (PLP)-dependent enzymes that cleave sulfur from cysteine via an enzyme cysteinyl persulfide intermediate. In vitro studies of these enzymes have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide intermediate, and it has been s...

journal_title：Biochemistry

authors： Behshad E,Parkin SE,Bollinger JM Jr

更新日期：2004-09-28 00:00:00

abstract：:In the presence of Ca2+ norchlorpromazine isothiocyanate forms a monocovalent complex with calmodulin: CAPP1-calmodulin (Newton et al, 1983). Trypsin digestion of [3H]CAPP1-calmodulin yields as the major radioactive peptide N epsilon-CAPP-Lys-Met-Lys, corresponding to residues 75-77 of calmodulin. Stoichiometric amoun...

journal_title：Biochemistry

authors： Newton DL,Klee CB

更新日期：1989-05-02 00:00:00

abstract：:l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

journal_title：Biochemistry

authors： Chow C,Hegde S,Blanchard JS

更新日期：2017-08-08 00:00:00