Abstract：
:A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potassium was critical for the synthesis of high molecular weight products with properties similar to pro-alpha-chains. The potassium concentration for optimal protein synthesis, as judged by maximum incorporation of [3H]proline into acid precipitable material, was considerably lower than the concentration required for the synthesis of high molecular weight collagenous peptides.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Benveniste K,Wilczek J,Ruggieri A,Stern Rdoi
10.1021/bi00649a016subject
Has Abstractpub_date
1976-02-24 00:00:00pages
830-5issue
4eissn
0006-2960issn
1520-4995journal_volume
15pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:To investigate the effect of a reduced level of selenocysteine (Sec) tRNA[Ser]Sec in selenoprotein biosynthesis, two mouse embryonic stem (ES) cell lines heterozygous for the corresponding gene were generated by homologous recombination of the host genome with targeting vectors encoding a deleted or a disrupted tRNA[S...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970608t
更新日期：1997-07-15 00:00:00
abstract：:The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00394a030
更新日期：1987-10-06 00:00:00
abstract：:The physicochemical and catalytic properties of thioredoxin-T' are described. This complemented protein structure consists of a 1:1 complex between the inactive fragments thioredoxin-T-(1--73) and thioredoxin T-(74--108). These are generated by selective trypsin cleavage at Arg-73 in lysine-modified and denatured Esch...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00592a010
更新日期：1979-12-11 00:00:00
abstract：:Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00685a027
更新日期：1975-07-15 00:00:00
abstract：:A conformational analysis of the valine side chains of ribonuclease T1 (RNase T1) was performed using NMR spectroscopy, in particular homonuclear (1H, 1H and 13C, 13C) and heteronuclear (1H, 15N and 1H, 13C) vicinal spin-spin coupling constants as obtained from E.COSY-type NMR experiments. The coupling constants relat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00184a017
更新日期：1994-05-10 00:00:00
abstract：:The relationship between steroid binding and protein subunit interactions of rabbit sex steroid binding protein (rSBP) has been studied by steady-state and time-resolved fluorescence spectroscopy. The high-affinity (Ka approximately 10(8) M-1 at 4 degrees C), fluorescent estrogen d-1,3,5(10),6,8-estrapentaene-3,17 bet...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00492a005
更新日期：1990-10-09 00:00:00
abstract：:The contact between EcoRI endonuclease and the "primary clamp" phosphate of its recognition site pGAATTC is absolutely required for recognition of the canonical and all variant DNA sites. We have probed this contact using oligonucleotides containing single stereospecific (Rp)- or (Sp)- phosphorothioates (Ps). At the G...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960261e
更新日期：1996-07-09 00:00:00
abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00600a014
更新日期：1978-04-04 00:00:00
abstract：:In order to evaluate the contributions that histidine residues might play both in the catalytic activities of endonuclease V and in binding to nontarget DNA, the technique of oligonucleotide site directed mutagenesis was used to create mutations at each of the four histidine residues in the endonuclease V gene. Althou...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00246a025
更新日期：1991-08-13 00:00:00
abstract：:The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00347a019
更新日期：1985-12-17 00:00:00
abstract：:The effect of long-term storage on the electronic absorption spectrum and the kinetic properties of cytochrome c peroxidase has been investigated. No detectable differences were observed between freshly isolated enzyme and enzyme stored below -20 degrees C, in the crystalline state, for up to 41 months. The electronic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00470a004
更新日期：1990-05-08 00:00:00
abstract：:The binding of UDP-N-acetylglucosamine (UDPNAG) to the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) was studied in the absence and presence of the antibiotic fosfomycin by isothermal titration calorimetry. Fosfomycin binds covalently to MurA in the presence of UDPNAG and also in its absence as demonst...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0107041
更新日期：2001-08-21 00:00:00
abstract：:Self-assembly of beta-sheet domains resulting in the formation of pathogenic, fibrillar protein aggregates (amyloids) is a characteristic feature of various medical disorders. These include neurodegenerative diseases, such as Alzheimer's, Huntington's, and Creutzfeldt-Jacob's. A significant problem in studying such ag...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990510+
更新日期：1999-06-29 00:00:00
abstract：:Site-directed mutagenesis, electron microscopy, and X-ray crystallography were used to probe the structural basis of annexin IV-induced membrane aggregation and the inhibition of this property by protein kinase C phosphorylation. Site-directed mutants that either mimic (Thr6Asp, T6D) or prevent (Thr6Ala, T6A) phosphor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002507s
更新日期：2001-04-03 00:00:00
abstract：:During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700416h
更新日期：2007-07-10 00:00:00
abstract：:HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9917535
更新日期：2000-01-18 00:00:00
abstract：:Polyisoprenyl diphosphates play diverse and vital roles in cell function in health and disease. The counter-regulatory lipid signaling molecule, presqualene diphosphate (PSDP), is rapidly converted to its monophosphate form (PSMP) upon cell activation [Levy, B. D., Petasis, N. A., and Serhan, C. N. (1997) Nature 389, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8020636
更新日期：2009-04-07 00:00:00
abstract：:The mechanism by which the heme-containing peroxidase, chloroperoxidase, is able to chlorinate substrates is poorly understood. One approach to advance our understanding of the mechanism of the enzyme is to determine those factors which contribute to its stability. In particular, under alkaline conditions, chloroperox...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961512m
更新日期：1996-11-19 00:00:00
abstract：:In a previously determined minimal kinetic scheme for DNA polymerization catalyzed by the Klenow fragment (KF) of Escherichia coli DNA polymerase I, a nonchemical step that interconverted the KF'.DNAn+1.PPi and KF.DNAn+1PPi complexes was not observed in correct incorporation [Kuchta, R. D., Mizrahi, V., Benkovic, P.A....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00234a002
更新日期：1991-05-21 00:00:00
abstract：:Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-associa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a018
更新日期：1976-10-19 00:00:00
abstract：:The nature of the active site cofactor and the amino acid sequence flanking this structure have been determined in a range of copper amine oxidases. For enzymes from porcine plasma, porcine kidney, and pea seedlings, proteolytic digestion was performed on phenylhydrazone or p-nitrophenylhydrazone derivatives. Thermoly...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00163a025
更新日期：1992-12-08 00:00:00
abstract：:We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00461a003
更新日期：1990-03-06 00:00:00
abstract：:We assigned l-talarate dehydratase (TalrD) and galactarate dehydratase (GalrD) functions to a group of orthologous proteins in the mechanistically diverse enolase superfamily, focusing our characterization on the protein encoded by the Salmonella typhimurium LT2 genome (GI:16766982; STM3697). Like the homologous mande...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7008882
更新日期：2007-08-21 00:00:00
abstract：:To test for on target toxicity of a new chemical entity, it is important to have comparable binding affinities of the compound in the target proteins from humans and the test species. To evaluate our myeloid cell leukemia-1 (Mcl-1) inhibitors, we tested them against rodent Mcl-1 and found a significant loss of binding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00626
更新日期：2018-08-14 00:00:00
abstract：:Pharaonis halorhodopsin (phR), the light-driven chloride ion pump from Natronobacterium pharaonis with C-terminal histidine tag, was expressed in Escherichia coli cells. The protein was solubilized with 0.1% n-dodecyl beta-D-maltopyranoside and purified with a nickel column. Removal of Cl- from the medium yields blue ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011788g
更新日期：2002-02-19 00:00:00
abstract：:Sensory rhodopsin II (SRII) is unique among the archaeal rhodopsins in having an absorption maximum near 500 nm, blue shifted roughly 70 nm from the other pigments. In addition, SRII displays vibronic structure in the lambda(max) absorption band, whereas the other pigments display fully broadened band maxima. The mole...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0116487
更新日期：2001-11-20 00:00:00
abstract：:Molecular dynamics simulations employing a molecular mechanics (MM) force field and hybrid quantum mechanics (QM) and MM (QM/MM) have been carried out to investigate the product specificity and mechanism of the histone H4 lysine 20 (H4-K20) methylation by human histone lysine methyltransferase SET8. At neutral pH, the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800244s
更新日期：2008-06-24 00:00:00
abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00832
更新日期：2020-12-29 00:00:00
abstract：:The functionality of the membrane-bound, ubiquinone-dependent pyruvate oxidase from the respiratory chain of Escherichia coli was reconstituted with a supported lipidic structure. The artificial structure was especially designed to allow the electrochemical control of the quinone pool through the lateral mobility of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002325y
更新日期：2001-02-06 00:00:00
abstract：:Adenosine 5'-phosphosulfate (APS) kinase catalyzes the second reaction in the two-step conversion of inorganic sulfate to 3'-phosphoadenosine 5'-phosphosulfate (PAPS). This report presents the 2.0 A resolution crystal structure of ligand-free APS kinase from the filamentous fungus, Penicillium chrysogenum. The enzyme ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9924157
更新日期：2000-02-22 00:00:00
