Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant.

abstract：:The preferential interactions of proteins with solvent components were studied in concentrated aqueous solutions of the sulfate, acetate, and chloride salts of Mg2+, Ba2+, Ca2+, Mn2+ and Ni2+ [except for CaSO4, BaSO4, Mn-(OAc)2, and Ni(OAc)2], and results were compared with those of the Na+ salts. It was found that, f...

journal_title：Biochemistry

authors： Arakawa T,Timasheff SN

更新日期：1984-12-04 00:00:00

abstract：:The difference spectra of binary and ternary complexes of horse liver alcohol dehydrogenase with oxidized and reduced nicotinamide adenine dinucleotides, nicotinamide 1,N6-ethenoadenine dinucleotide, and adenosine diphosphate ribose along with a number of substrate analogues have been measured. These spectra bear a ve...

journal_title：Biochemistry

authors： Subramanian S,Ross JB,Ross PD,Brand L

更新日期：1981-07-07 00:00:00

abstract：:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in propo...

journal_title：Biochemistry

authors： Granados EN,Bello J

更新日期：1980-07-08 00:00:00

abstract：:We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...

journal_title：Biochemistry

authors： Forman HJ,Maiorino M,Ursini F

更新日期：2010-02-09 00:00:00

abstract：:The kinetic and equilibrium CO binding properties of hemoglobin (Hb) M Iwate (alpha2 87 His leads to Tyr beta2) have been investigated. The results show that the alpha(Met) beta2 (CO) tetramer of this protein has a low affinity for CO, as indicated by the stopped-flow and flash-photolysis kinetic, as well as the CO bi...

journal_title：Biochemistry

authors： Salhany JM,Castillo CL,Ogawa S

更新日期：1976-11-30 00:00:00

abstract：:The one-for-one exchange of Cl(-) and HCO(3)(-) ions is catalyzed by human erythrocyte anion exchanger 1 (hAE1) through a ping-pong mechanism whereby the protein exists in two main conformations, with the single anion-binding site exposed at either the cytoplasmic (inner) side (E(i)) or the extracellular side (E(o)), ...

journal_title：Biochemistry

authors： Pal P,Lebedev D,Salim S,Knauf PA

更新日期：2006-05-23 00:00:00

abstract：:Effects of different end sequences on melting, circular dichroism spectra (CD), and enzyme binding properties were investigated for four 40 base pair, non-self-complementary duplex DNA oligomers. The center sequences of these oligoduplexes have either of two 22 base pair modules flanked on both sides by sequences diff...

journal_title：Biochemistry

authors： Vallone PM,Benight AS

更新日期：2000-07-04 00:00:00

abstract：:To understand the details of rate limitation of turnover of the photosynthetic reaction center, photooxidation of horse heart cytochrome c by reaction center from Rhodobacter spheroides in detergent dispersion has been examined by intense continuous illumination under a wide variety of conditions of cytochrome concent...

journal_title：Biochemistry

authors： Gerencsér L,Laczkó G,Maróti P

更新日期：1999-12-21 00:00:00

abstract：:The structures of the modified folates present in Pyrococcus furiosus have been determined. This was accomplished largely by the characterization of the arylamines resulting from the air oxidative cleavage of the reduced modified folates present in these cells, using both chemical and enzymatic methods. Cell extracts ...

journal_title：Biochemistry

authors： White RH

更新日期：1993-01-26 00:00:00

abstract：:The homogeneous, single-walled phosphatidylcholine-cholesterol mixed vesicles were prepared by ultrasonic irradiation of egg phosphatidylcholine in the presence of various amounts of cholesterol in solution at 4 degrees under a nitrogen atmosphere followed by molecular sieve chromatography on a Sepharose 4B column. Ph...

journal_title：Biochemistry

authors： Newman GC,Huang C

更新日期：1975-07-29 00:00:00

abstract：:In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...

journal_title：Biochemistry

authors： Breton J

更新日期：2007-04-17 00:00:00

abstract：:The amino acid sequence of human alpha-fetoprotein, a 67-kDa protein present in mammalian embryonic serum, was verified by fast atom bombardment mass spectrometric (FAB/MS) analyses of three different enzymatic digests of the protein. Human alpha-fetoprotein obtained from a large-scale cell culture was digested with t...

journal_title：Biochemistry

authors： Pucci P,Siciliano R,Malorni A,Marino G,Tecce MF,Ceccarini C,Terrana B

更新日期：1991-05-21 00:00:00

abstract：:We report here that the DNA strand scission by dynemicin A is not only sequence-specific but also conformation-specific. The salt-induced B----Z conformational transition dramatically enhanced the cleavage by dynemicin A in a B-Z junction region. By contrast, the bleomycin-Fe(II) complex, the elsamicin A-Fe(II) comple...

journal_title：Biochemistry

authors： Ichikawa A,Kuboya T,Aoyama T,Sugiura Y

更新日期：1992-07-28 00:00:00

abstract：:The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane patients. As part of an alternative approach to determine of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the ...

journal_title：Biochemistry

authors： Yeagle PL,Alderfer JL,Albert AD

更新日期：1995-11-14 00:00:00

abstract：:Trimethylamine dehydrogenase (TMADH) from the bacterium Methylophilus methylotrophus (sp. W(3)A(1)) and its C30A mutant were inactivated with three known inactivators of monoamine oxidase, namely, phenylhydrazine, N-cyclopropyl-alpha-methylbenzylamine, and 1-phenylcyclopropylamine. All three compounds irreversibly ina...

journal_title：Biochemistry

authors： Mitchell DJ,Nikolic D,Jang MH,van Breemen RB,Hille R,Silverman RB

更新日期：2001-07-24 00:00:00

abstract：:An adenosine cyclic 3',5'-monophosphate (cAMP) dependent protein kinase has recently been shown to exist in Dictyostelium discoideum and to be developmentally regulated. In this report we have followed the chromatographic behavior of both the holoenzyme and its subunits. A cAMP-dependent holoenzyme could be obtained f...

journal_title：Biochemistry

authors： Rutherford CL,Vaughan RL,Cloutier MJ,Ferris DK,Brickey DA

更新日期：1984-09-25 00:00:00

abstract：:Properties of the mycobacterial polymethylpolysaccharide-lipid complex have been investigated by fluorometric techniques. From the dissociation constant for the O-methyglucose polysaccharide-parinaric acid complex at 293 K, a Gibbs free energy (delta G degree) of -33.65 kJ/mol was obtained. The Kd decreased with incre...

journal_title：Biochemistry

authors： Kiho T,Ballou CE

更新日期：1988-07-26 00:00:00

abstract：:We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...

journal_title：Biochemistry

authors： Kloc M,Li XX,Etkin LD

更新日期：1993-08-17 00:00:00

abstract：:Endogenous lectins induce effects on cell growth by binding to antennae of natural glycoconjugates. These complex carbohydrates often present more than one potential lectin-binding site in a single chain. Using the growth-regulatory interaction of the pentasaccharide of ganglioside GM(1) with homodimeric galectin-1 on...

journal_title：Biochemistry

authors： Siebert HC,André S,Lu SY,Frank M,Kaltner H,van Kuik JA,Korchagina EY,Bovin N,Tajkhorshid E,Kaptein R,Vliegenthart JF,von der Lieth CW,Jiménez-Barbero J,Kopitz J,Gabius HJ

更新日期：2003-12-23 00:00:00

abstract：:Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. AHAS isozyme II from Escherichia coli is spec...

journal_title：Biochemistry

authors： Vyazmensky M,Steinmetz A,Meyer D,Golbik R,Barak Z,Tittmann K,Chipman DM

更新日期：2011-04-19 00:00:00

abstract：:The energy derived from optimized van der Waals interactions in closely packed, folded proteins has been proposed to be of similar energetic magnitude to hydrophobicity in stabilizing the native state. If packing is this energetically important, it should influence the evolution of protein core sequences. To test this...

journal_title：Biochemistry

authors： Chen J,Stites WE

更新日期：2001-12-18 00:00:00

abstract：:The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one a...

journal_title：Biochemistry

authors： Poulos TL,Finzel BC,Howard AJ

更新日期：1986-09-09 00:00:00

abstract：:In this study, we have mapped the intracellular alkylation sites of adozelesin and bizelesin, two potent analogs of CC-1065, in individual genes at the single-nucleotide level. Human colon carcinoma cells were treated with adozelesin and bizelesin, and the position of adducts were mapped within the PGK-1 and p53 genes...

journal_title：Biochemistry

authors： Lee CS,Pfeifer GP,Gibson NW

更新日期：1994-05-17 00:00:00

abstract：:The association of ferredoxin with ferredoxin:NADP+ reductase (both proteins from spinach chloroplasts) was characterized by isothermal titration calorimetry and fluorescence quenching titration. The formation of the complex is mainly driven by a positive entropy change (delta S = 125 +/- 8 J mol-1 K-1). The calorimet...

journal_title：Biochemistry

authors： Jelesarov I,Bosshard HR

更新日期：1994-11-15 00:00:00

abstract：:S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Ba...

journal_title：Biochemistry

authors： Rustandi RR,Baldisseri DM,Inman KG,Nizner P,Hamilton SM,Landar A,Landar A,Zimmer DB,Weber DJ

更新日期：2002-01-22 00:00:00

abstract：:Insulin decreases multifunctional protein kinase (MFPK) activity in rat adipose tissue [Ramakrishna, S., & Benjamin, W. B. (1988) J. Biol. Chem. 263, 12677-12681]. Insulin also decreases the phosphorylation of peptide B but increases the phosphorylation of peptide A of ATP-citrate lyase (ATP-CL). The mechanism for thi...

journal_title：Biochemistry

authors： Ramakrishna S,Murthy KS,Benjamin WB

更新日期：1989-01-24 00:00:00

abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

journal_title：Biochemistry

authors： Jelicks LA,Broido MS,Becker JM,Naider FR

更新日期：1989-05-16 00:00:00

abstract：:p21-activated kinases (PAKs) serve as effector proteins for the GTP-binding proteins Cdc42 and Rac. They are serine/threonine kinases containing the Cdc42/Rac interactive binding (CRIB) motif. The main aim of this study was to define the minimal domain of alphaPAK required for Cdc42/Rac binding. Eight stable PAK fragm...

journal_title：Biochemistry

authors： Thompson G,Owen D,Chalk PA,Lowe PN

更新日期：1998-05-26 00:00:00

abstract：:N-(1-Pyrene)maleimide is nonfluorescent in aqueous solution but forms strongly fluorescent adducts with sulfhydryl groups of organic compounds or proteins. The conjugation reactions of N-(1-pyrene)maleimide are relatively fast and can be monitored by the increase in fluorescence intensity of the pyrene chromophore. In...

journal_title：Biochemistry

authors： Wu CW,Yarbrough LR

更新日期：1976-06-29 00:00:00

abstract：:The local and global dynamics of the chemokine receptor CXCR1 are characterized using a combination of solution NMR and solid-state NMR experiments. In isotropic bicelles (q = 0.1), only 13% of the expected number of backbone amide resonances is observed in (1)H/(15)N HSQC solution NMR spectra of uniformly (15)N-label...

journal_title：Biochemistry

authors： Park SH,Casagrande F,Das BB,Albrecht L,Chu M,Opella SJ

更新日期：2011-03-29 00:00:00