Abstract：
:The interaction of poly(N epsilon, N epsilon, N epsilon-trimethyl-L-lysine) ([Lys(Me3)]n) and poly(N delta, N delta, N delta-trimethyl-L-ornithine) ([Orn(Me3)]n) with polynucleotides was studied by thermal denaturation, viscosity, and dissociation by salt. The methylated polymers decrease the viscosity of DNA in proportion to the amount of bound peptide. [Lys(Me3)]n and [Orn(Me3)]n raise Tm of polynucleotides more than do (Lys)n and (Orn)n. Dissociation of the polypeptide-polynucleotide complexes with NaCl, KCl, or MgCl2 required about half the salt concentration for the methylated polymers as for the parent polymers. The effects of Tm on DNA appear to be complex and may involve differences in the hydrophobic effects, solvation, and conformational entropy. The salt dissociation data are discussed in relation t the role of histone methylation in chromatin function.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Granados EN,Bello Jdoi
10.1021/bi00555a020subject
Has Abstractpub_date
1980-07-08 00:00:00pages
3227-33issue
14eissn
0006-2960issn
1520-4995journal_volume
19pub_type
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