Abstract:
:The difference spectra of binary and ternary complexes of horse liver alcohol dehydrogenase with oxidized and reduced nicotinamide adenine dinucleotides, nicotinamide 1,N6-ethenoadenine dinucleotide, and adenosine diphosphate ribose along with a number of substrate analogues have been measured. These spectra bear a very close resemblance to those obtained by perturbation of the coenzyme(s) and their analogues by acid, NaCl, dioxane, or tert-butyl alcohol. It is inferred that the coenzymes experience a combination of ionic and nonpolar environments at the adenine binding site of the enzyme. This is borne out by published X-ray crystallographic results. The phosphorescence spectra do not indicate the presence of ionized tyrosine in ternary complexes invovling enzyme, coenzyme, and substrate analogues. The ultraviolet spectra can be explained as arising from the perturbation of the coenzyme chromophores upon binding to the enzyme without having to invoke tyrosine ionization.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Subramanian S,Ross JB,Ross PD,Brand Ldoi
10.1021/bi00517a022subject
Has Abstractpub_date
1981-07-07 00:00:00pages
4086-93issue
14eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Prenyltransferase (EC 2.5.1.1) has been obtained from chicken liver in a stable crystalline form. The enzyme has been shown to be homogeneous by polyacrylamide gel electrophoresis at pH 8.4, and by electrophoresis in sodium dodecyl sulfate containing gels. Electrofocusing of the crystalline enzyme results in a single ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00672a009
更新日期:1975-01-14 00:00:00
abstract::An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026191v
更新日期:2002-10-15 00:00:00
abstract::The identity of the sites of insulin-stimulated serine phosphorylation in the human insulin receptor was examined by synthesizing peptides that together encompassed all the serine residues of the cytosolic portion of the beta-subunit and testing them as substrates for phosphorylation by a preparation of human insulin ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00029a025
更新日期:1995-07-25 00:00:00
abstract::To study fibronectin (FN) conformation and assembly, we generated several deletion mutants: FNΔI1-5, FNΔIII1-3, FNΔIII4-8, and FNΔIII11-14. A monomeric form, FNmono, which lacked the C-terminal dimerization region, was also created. FNtnA-D was generated by swapping FNIII domains 1-8 in FNΔIII11-14 with seven FNIII do...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00589
更新日期:2017-08-29 00:00:00
abstract::Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00274a011
更新日期:1983-03-01 00:00:00
abstract::The backbone dynamics of uniformly 15N-labeled interleukin-1 beta are investigated by using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. 15N T1, T2, and NOE data at a spectrometer frequency of 600 MHz are obtained for 90% of the backbone amide groups. The data provide evidence for motions on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00484a006
更新日期:1990-08-14 00:00:00
abstract::In previous studies [van Wijk, K. J., Bingsmark, S., Aro, E.-M., & Andersson, B. (1995) J. Biol. Chem. 270, 25685-25695; van Wijk, K. J., Andersson, B., & Aro, E.-M. (1996) J. Biol. Chem 271, 9627-9636], we have demonstrated that D1 protein synthesized in isolated chloroplasts and thylakoids is incorporated into the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962921l
更新日期:1997-05-20 00:00:00
abstract::The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801651w
更新日期:2009-01-13 00:00:00
abstract::The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a001
更新日期:1994-05-31 00:00:00
abstract::The catalytically competent active-site structure of a true acylenzyme reaction intermediate of TEM-1 beta-lactamase formed with the kinetically specific spin-labeled substrate 6-N-(2,2,5,5-tetramethyl-1-oxypyrrolinyl-3-carboxyl)-penicillanic acid isolated under cryoenzymologic conditions has been determined by angle-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0021075
更新日期:2001-02-27 00:00:00
abstract::The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00600a014
更新日期:1978-04-04 00:00:00
abstract::The photoaffinity probes [gamma-32P]2-azidoATP (2-N3ATP) and [alpha-32P]8-azido-ATP (8-N3ATP) were used to investigate the binding of ATP to highly purified 2-5A synthetase. 2-N3ATP and 8-N3ATP are substrates for 2-5A synthetase [Suhadolnik, R.J., Karikó, K., Sobol, R.W., Jr., Li, S.W., Reichenbach, N.L., & Haley, B.E...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00424a024
更新日期:1988-11-29 00:00:00
abstract::A systematic 220-MHz proton nuclear magnetic resonance (nmr) study has been made of all common purine and pyrimidine 2'(3')-ribonucleotides in D2O solutions at 20 plus or minus 2 degrees. Spectra for the entire series were measured under similar conditions of concentration, temperature, and ionic strength, thereby fac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00674a013
更新日期:1975-02-11 00:00:00
abstract::The melanocortin-4 (MC4) receptor is a potential therapeutic target for obesity and cachexia, for which nonpeptide agonists and antagonists are being developed, respectively. The aim of this study was to identify molecular interactions between the MC4 receptor and nonpeptide ligands, and to compare the mechanism of bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051316s
更新日期:2005-11-08 00:00:00
abstract::Oxidation of the biological antioxidant alpha-tocopherol (vitamin E; TH) by peroxyl radicals yields 8a-(alkyldioxy)tocopherones, which either may hydrolyze to alpha-tocopheryl quinone (TQ) or may be reduced by ascorbic acid to regenerate TH. To define the chemistry of this putative two-electron TH redox cycle, we stud...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00451a034
更新日期:1989-12-12 00:00:00
abstract::The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9808971
更新日期:1998-11-03 00:00:00
abstract::Botulinum neurotoxins (BoNTs) elicit flaccid paralysis through cleavage of SNARE proteins within peripheral neurons. There are seven serotypes of the BoNTs, termed A-G, which differ in the SNARE protein and/or site that is cleaved. BoNTs are single-chain toxins that comprise an N-terminal zinc metalloprotease domain t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602396x
更新日期:2007-03-20 00:00:00
abstract::Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0524060
更新日期:2006-04-04 00:00:00
abstract::Binding of Ca(2+)-activated calmodulin (Ca(2+)-CaM) to neuronal nitric-oxide synthase (nNOS) increases the rate of 2,6-dichloroindolphenol (DCIP) reduction 2-3-fold and that of cytochrome c(3+) 10-20-fold. Parallel initial velocity patterns indicated that both substrates were reduced via two-half reactions in a ping-p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0023495
更新日期:2001-04-17 00:00:00
abstract::The temperature dependence of biological rates at different scales (from individual enzymes to isolated organisms to ecosystem processes such as soil respiration and photosynthesis) is the subject of much historical and contemporary research. The precise relationship between the temperature dependence of enzyme rates ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00530
更新日期:2020-09-29 00:00:00
abstract::The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900038p
更新日期:2009-03-24 00:00:00
abstract::Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9000062
更新日期:2009-06-23 00:00:00
abstract::Glyoxylate carboligase (GCL) is a thiamin diphosphate (ThDP)-dependent enzyme, which catalyzes the decarboxylation of glyoxylate and ligation to a second molecule of glyoxylate to form tartronate semialdehyde (TSA). This enzyme is unique among ThDP enzymes in that it lacks a conserved glutamate near the N1' atom of Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi300893v
更新日期:2012-10-09 00:00:00
abstract::The effects of ionophore A23187 on the movements of Ca2+, Mg2+, H+ phosphate, and succinate and its effects on energy utilization by mitochondria have been studied as a function of ionophore concentrations. At a low ratio of the compound to mitochondrial protein, below that required for maximal uncoupling, an apparent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00657a032
更新日期:1976-06-15 00:00:00
abstract::The key polyamine catabolizing enzyme spermidine-spermine N1-acetyltransferase (SSAT) is among the few genes known to be inducible by the natural polyamines. Certain polyamine analogs markedly exaggerate this response and thus provide useful tools for studying the underlying regulatory mechanisms. As shown here, the a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9612273
更新日期:1996-11-12 00:00:00
abstract::Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00216a035
更新日期:1991-01-15 00:00:00
abstract::RNA repeat expansions cause a host of incurable, genetically defined diseases. The most common class of RNA repeats consists of trinucleotide repeats. These long, repeating transcripts fold into hairpins containing 1 × 1 internal loops that can mediate disease via a variety of mechanism(s) in which RNA is the central ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00252
更新日期:2017-07-11 00:00:00
abstract::Fluorescein 5'-isothiocyanate (FITC) was used to modify the lysine residues of bovine transducin (T), a GTP-binding protein involved in phototransduction of rod photoreceptor cells. The incorporation of FITC showed a stoichiometry of approximately 1 mol of FITC/mol of transducin. The labeling was specific for the T al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00380a022
更新日期:1987-03-24 00:00:00
abstract::Tropomyosin (TM) is a coiled-coil that binds head-to-tail along the helical actin filament. The ends of 284-residue tropomyosins are believed to overlap by about nine amino acids. The present study investigates the function of the N- and C-terminal overlap regions. Recombinant tropomyosins were produced in Escherichia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000242b
更新日期:2000-06-13 00:00:00
abstract::The causes of the salting-in of beta-lactoglobulin by glycine and NaCl, a solubility behavior contrary to expectations, were probed by a detailed study of the interactions between these solvent components and the protein. The preferential interactions of beta-lactoglobulin with solvent components in aqueous glycine an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00390a038
更新日期:1987-08-11 00:00:00