Crystallization and partial characterization of prenyltransferase from avian liver.

abstract：:Two series of experiments were carried out to characterize (a) peptide fragments of sarcoplasmic reticulum (SR) ATPase, based on proteolysis with different enzymes and distribution of known labels, and (b) specific labeling and functional inactivation patterns, following ATPase derivatization with dicyclohexylcarbodii...

journal_title：Biochemistry

authors： de Ancos JG,Inesi G

更新日期：1988-03-08 00:00:00

abstract：:The preparation of native alpha- and beta-chains of carp hemoglobin and the preparation of the hybrid hemoglobin alpha(carp):beta(human), hybrid I, are described. CO association and dissociation kinetics were determined for the hybrid hemoglobin alpha(carp):beta(human) as a function of fractional saturation by stopped...

journal_title：Biochemistry

authors： Goss DJ,Parkhurst LJ

更新日期：1984-05-08 00:00:00

abstract：:Studies are presented of the equilibrium binding of [3H]-d-tubocurarine (dTC) and [3H]acetylcholine (AcCh) to Torpedo postsynaptic membranes. The saturable binding of [3H]dTC is characterized by two affinities: Kd1 = 33 +/- 6 nM and Kd2 = 7.7 +/- 4.6 microM, with equal numbers of binding sites. Both components are com...

journal_title：Biochemistry

authors： Neubig RR,Cohen JB

更新日期：1979-11-27 00:00:00

abstract：:The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...

journal_title：Biochemistry

authors： Haddad JG,Hu YZ,Kowalski MA,Laramore C,Ray K,Robzyk P,Cooke NE

更新日期：1992-08-11 00:00:00

abstract：:NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...

journal_title：Biochemistry

authors： Murataliev MB,Feyereisen R

更新日期：2000-10-17 00:00:00

abstract：:Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...

journal_title：Biochemistry

authors： Ohgaki R,van IJzendoorn SC,Matsushita M,Hoekstra D,Kanazawa H

更新日期：2011-02-01 00:00:00

abstract：:The ligand-binding dynamics and the reaction with O(2) of the fully (five-electron) reduced cytochrome caa(3) from the thermohalophilic bacterium Rhodothermus (R.) marinus were investigated. The enzyme is a proton pump which has all the residues of the proton-transfer pathways found in the mitochondrial-like enzymes c...

journal_title：Biochemistry

authors： Sigurdson H,Namslauer A,Pereira MM,Teixeira M,Brzezinski P

更新日期：2001-09-04 00:00:00

abstract：:Dynemicin A (1), a member of the enediyne family of natural products, binds to double-stranded DNA (K(B) approximately 10(4) M(-1)) and in the presence of millimolar concentrations of a reducing cofactor such as NADPH or GSH reacts to cleave DNA. In this work, we show that the two flavin-based enzymes ferredoxin-NADP+...

journal_title：Biochemistry

authors： Myers AG,Kort ME,Cohen SB,Tom NJ

更新日期：1997-04-01 00:00:00

abstract：:We examined the interactions of nucleotides with the CMP-sialic acid transporter in order to better understand which features play a role in binding and to investigate the relationship between binding and subsequent transport. With respect to the sugar, the transporter requires a complete ribose ring for tight binding...

journal_title：Biochemistry

authors： Chiaramonte M,Koviach JL,Moore C,Iyer VV,Wagner CR,Halcomb RL,Miller W,Melançon P,Kuchta RD

更新日期：2001-11-27 00:00:00

abstract：:Galactose oxidase is a remarkable enzyme containing a metalloradical redox cofactor capable of oxidizing a variety of primary alcohols during enzyme turnover. Recent studies using 1-O-methyl alpha-D-galactopyranoside have revealed an unusually large kinetic isotope effect (KIE) for oxidation of the alpha-deuterated al...

journal_title：Biochemistry

authors： Whittaker MM,Whittaker JW

更新日期：2001-06-19 00:00:00

abstract：:Several models for how amino acid substitutions in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) confer resistance to chloroquine (CQ) and other antimalarial drugs have been proposed. Distinguishing between these models requires detailed analysis of high-resolution CQ transport data that is unfo...

journal_title：Biochemistry

authors： Cabrera M,Natarajan J,Paguio MF,Wolf C,Urbach JS,Roepe PD

更新日期：2009-10-13 00:00:00

abstract：:Plants employ a variety of light, oxygen, voltage (LOV) domain photoreceptors to regulate diverse aspects of growth and development. The Zeitlupe (ZTL), Flavin-Kelch-Fbox-1 (FKF1), and LOV-Kelch-Protein-2 (LKP2) proteins dictate measurement of the day length, flowering time, and regulation of the circadian clock by bl...

journal_title：Biochemistry

authors： Pudasaini A,Zoltowski BD

更新日期：2013-10-08 00:00:00

abstract：:Isolated chloroplasts are capable of synthesizing chloroplast DNA in the presence of Mg2+ and deoxynucleoside triphosphates. The in vitro reaction proceeds for at least 60 min and is inhibited by KC1 and N-ethylmaleimide. Stretches of several hundred nucleotides in length are synthesized within an hour. Little or no i...

journal_title：Biochemistry

authors： Zimmermann W,Weissbach A

更新日期：1982-07-06 00:00:00

abstract：:The complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) (EC 2.6.1.2) is presented. Two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and Achromobacter protease I, respectively. Isolated...

journal_title：Biochemistry

authors： Ishiguro M,Takio K,Suzuki M,Oyama R,Matsuzawa T,Titani K

更新日期：1991-10-29 00:00:00

abstract：:Cystathionine β-synthase (CBS) catalyzes the first step in the transsulfuration pathway in mammals, i.e., the condensation of serine and homocysteine to produce cystathionine and water. Recently, we have reported a steady-state kinetic analysis of the three hydrogen sulfide (H(2)S)-generating reactions that are cataly...

journal_title：Biochemistry

authors： Singh S,Ballou DP,Banerjee R

更新日期：2011-01-25 00:00:00

abstract：:The reversible decline of the nicotinic acetylcholine receptor's response to acetylcholine during prolonged exposure to acetylcholine is known as desensitization. Here, we studied ethanol's modulation of fast agonist-induced desensitization of the nicotinic acetylcholine receptor in postsynaptic membrane vesicles from...

journal_title：Biochemistry

authors： Wu G,Miller KW

更新日期：1994-08-09 00:00:00

abstract：:Paramagnetic agents produce line broadening and thus cancellation of anti phase cross-peak components in two-dimensional correlated nuclear magnetic resonance spectra. The specificity of this effect was examined to determine its utility for identifying surface residues of proteins. Ubiquitin and hen egg white lysozyme...

journal_title：Biochemistry

authors： Petros AM,Mueller L,Kopple KD

更新日期：1990-10-30 00:00:00

abstract：:A slow conversion of adenine residues to hypoxanthine occurs in single-stranded DNA when heated in neutral aqueous buffers. The rate of this reaction at pH 7.6 and 110 degrees C is k = 4 x 10(-8) s-1, as determined by base analysis of heat-treated DNA that contains radioactively labeled adenine residues. It is propose...

journal_title：Biochemistry

authors： Karran P,Lindahl T

更新日期：1980-12-23 00:00:00

abstract：:The studies reported here were undertaken to determine whether the thrombin precursors prothrombin, prethrombin 1, prethrombin 2, and Meizo thrombin interact with the fluorescent, reversible thrombin inhibitor dansylarginine N,N-(3-ethyl-1,5-pentanediyl)amide (DAPA) [Nesheim, M. E., Prendergast, F. G., & Mann, K. G. (...

journal_title：Biochemistry

authors： Hibbard LS,Nesheim ME,Mann KG

更新日期：1982-05-11 00:00:00

abstract：:Apical membrane antigen 1 (AMA1) of the human malaria parasite Plasmodium falciparum is synthesized by schizont stage parasites and has been implicated in merozoite invasion of host erythrocytes. Phage-display techniques have recently been used to identify two 15-residue peptides, F1 and F2, which bind specifically to...

journal_title：Biochemistry

authors： Keizer DW,Miles LA,Li F,Nair M,Anders RF,Coley AM,Foley M,Norton RS

更新日期：2003-08-26 00:00:00

abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...

journal_title：Biochemistry

authors： Echtay KS,Bienengraeber M,Klingenberg M

更新日期：1997-07-08 00:00:00

abstract：:Deoxycytidylate (dCMP) hydroxymethylase (CH) catalyzes the formation of 5-(hydroxymethyl)-dCMP, essential for DNA synthesis in phage T4, from dCMP and methylenetetrahydrofolate (CH2THF). The nucleotide analog 5-fluorodeoxuridylate (FdUMP) stoichiometrically inactivates CH by formation of a covalent complex containing ...

journal_title：Biochemistry

authors： Graves KL,Hardy LW

更新日期：1994-11-08 00:00:00

abstract：:Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...

journal_title：Biochemistry

authors： Beechem JM,Sherman MA,Mas MT

更新日期：1995-10-24 00:00:00

abstract：:Picosecond transient absorption difference spectroscopic experiments were performed on membranes of the antenna/reaction center complex of Heliobacillus mobilis to study the electron transfer processes. Particular emphasis was placed on the blue spectral region, where the difference spectra of iron-sulfur centers and ...

journal_title：Biochemistry

authors： Lin S,Chiou HC,Blankenship RE

更新日期：1995-10-03 00:00:00

abstract：:Only beta-beta cross-links form when the alpha(3)(betaE(395)C)(3)gammaK(36)C (MF(1) residue numbers) double mutant subcomplex of TF(1), the F(1)-ATPase from the thermophilic Bacillus PS3, is slowly inactivated with CuCl(2) in the presence or absence of MgATP. The same slow rate of inactivation and extent of beta-beta ...

journal_title：Biochemistry

authors： Bandyopadhyay S,Allison WS

更新日期：2004-03-09 00:00:00

abstract：:Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...

journal_title：Biochemistry

authors： Spooner PJ,Watts A

更新日期：1991-04-23 00:00:00

abstract：:The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction of the actin-S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and N-hydroxysuccinimide generated pro...

journal_title：Biochemistry

authors： Van Dijk J,Fernandez C,Chaussepied P

更新日期：1998-06-09 00:00:00

abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...

journal_title：Biochemistry

authors： Serrano P,Hammond JA,Geralt M,Wüthrich K

更新日期：2018-03-13 00:00:00

abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...

journal_title：Biochemistry

authors： Brandes R,Vold RR,Vold RL,Kearns DR

更新日期：1986-11-18 00:00:00

abstract：:Ferredoxin I from Desulfovibrio africanus (Da FdI) is a small acidic [4Fe-4S] cluster protein that exchanges electrons with pyruvate-ferredoxin oxidoreductase (PFOR), a key enzyme in the energy metabolism of anaerobes. The thermodynamic properties and the electron transfer between PFOR and either native or mutated FdI...

journal_title：Biochemistry

authors： Pieulle L,Nouailler M,Morelli X,Cavazza C,Gallice P,Blanchet S,Bianco P,Guerlesquin F,Hatchikian EC

更新日期：2004-12-14 00:00:00