Human secreted carbonic anhydrase: cDNA cloning, nucleotide sequence, and hybridization histochemistry.


:Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, kidney, liver, and prostate gland. Hybridization histochemistry of human salivary glands shows mRNA for CA VI located in the acinar cells of these glands. The cDNA clones encode a protein of 308 amino acids that includes a 17 amino acid leader sequence typical of secreted proteins. The mature protein has 291 amino acids compared to 259 or 260 for the cytoplasmic isozymes, with most of the extra amino acids present as a carboxyl terminal extension. In comparison, sheep CA VI has a 45 amino acid extension [Fernley, R. T., Wright, R. D., & Coghlan, J. P. (1988b) Biochemistry 27, 2815]. Overall the human CA VI protein has a sequence identity of 35% with human CA II, while residues involved in the active site of the enzymes have been conserved. The human sheep secreted carbonic anhydrases have a sequence identity of 72%. This includes the two cysteine residues that are known to be involved in an intramolecular disulfide bond in the sheep CA VI. The enzyme is known to be glycosylated and three potential N-glycosylation sites (Asn-X-Thr/Ser) have been identified. Two of these are known to be glycosylated in sheep CA VI. Southern analysis of human DNA indicates that there is only one gene coding for CA VI.






Aldred P,Fu P,Barrett G,Penschow JD,Wright RD,Coghlan JP,Fernley RT




Has Abstract


1991-01-15 00:00:00












  • Isolation and characterization of bovine factor VII.

    abstract::Factor VII (proconvertin) has been purified approximately 5 x 10(5)-fold from bovine plasma with an overall yield of 30%. The isolation procedure involves barium sulfate adsorption and elution, DEAE-Sephadex batchwise adsorption and elution, benzamidine-agarose column chromatography, heparin-agarose column chromatogra...


    pub_type: 杂志文章


    authors: Kisiel W,Davie EW

    更新日期:1975-11-04 00:00:00

  • Tandem affinity purification in transgenic mouse embryonic stem cells identifies DDOST as a novel PPP1CC2 interacting protein.

    abstract::Members of the PP1 family of protein phosphatases achieve functional diversity through numerous and varied protein-protein interactions. In mammals, there are four PP1 isoforms, the ubiquitously expressed PPP1CA, PPP1CB, and PPP1CC1, and the testis specific splice isoform PPP1CC2. When the mouse Ppp1cc gene is deleted...


    pub_type: 杂志文章


    authors: MacLeod G,Varmuza S

    更新日期:2012-12-04 00:00:00

  • Comparison of the effects of tropomyosin and troponin-tropomyosin on the binding of myosin subfragment 1 to actin.

    abstract::The effect of tropomyosin alone on the binding of myosin subfragment 1 (S-1) to actin was compared to that of troponin-tropomyosin, with and without Ca2+. Over a range of ionic strength and with different ligands (adenyl-5'-yl imidodiphosphate, pyrophosphate, and adenosine 5'-diphosphate), tropomyosin confers slight c...


    pub_type: 杂志文章


    authors: Williams DL Jr,Greene LE

    更新日期:1983-05-24 00:00:00

  • Crystal structures and iron release properties of mutants (K206A and K296A) that abolish the dilysine interaction in the N-lobe of human transferrin.

    abstract::Human transferrin (Tf) is responsible for the binding and transport of iron in the bloodstream of vertebrates. Delivery of this bound iron to cells occurs by a process of receptor-mediated endocytosis during which Tf releases its iron at the reduced endosomal pH of approximately 5.6. Iron release from Tf involves a la...


    pub_type: 杂志文章


    authors: Nurizzo D,Baker HM,He QY,MacGillivray RT,Mason AB,Woodworth RC,Baker EN

    更新日期:2001-02-13 00:00:00

  • Disentangling the web of allosteric communication in a homotetramer: heterotropic activation in phosphofructokinase from Escherichia coli.

    abstract::Phosphofructokinase from Escherichia coli (EcPFK) is a homotetramer with four active sites and four allosteric sites. Understanding the allosteric activation of EcPFK by MgADP has been complicated by the complex web of possible interactions, including active site homotropic interactions, allosteric site homotropic int...


    pub_type: 杂志文章


    authors: Fenton AW,Paricharttanakul NM,Reinhart GD

    更新日期:2004-11-09 00:00:00

  • Mechanism of proton transfer in the isomerization of 5-androstene-3, 17-dione by 3-oxo-delta 5-steroid isomerase and its D38E mutant.

    abstract::The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...


    pub_type: 杂志文章


    authors: Zawrotny ME,Hawkinson DC,Blotny G,Pollack RM

    更新日期:1996-05-21 00:00:00

  • 19F nuclear magnetic resonance studies of selectively fluorinated derivatives of G- and F-actin.

    abstract::G-Actin is a globular protein (Mr 42 300) known to have three cysteine residues that are at least partially exposed and chemically reactive (Cys-10, -284, and -374). When G-actin was reacted with 3-bromo-1,1,1-trifluoropropanone, three resolvable 19F resonances were observed in the 19F NMR spectrum. This fluorinated G...


    pub_type: 杂志文章


    authors: Brauer M,Sykes BD

    更新日期:1986-04-22 00:00:00

  • Tyr275 and Lys279 stabilize NADPH within the catalytic site of NADPH:protochlorophyllide oxidoreductase and are involved in the formation of the enzyme photoactive state.

    abstract::Fluorescence spectroscopic and kinetic analysis of photochemical activity, cofactor and substrate binding, and enzyme denaturation studies were performed with highly purified, recombinant pea NADPH:protochlorophyllide oxidoreductase (POR) heterologously expressed in Escherichia coli. The results obtained with an indiv...


    pub_type: 杂志文章


    authors: Lebedev N,Karginova O,McIvor W,Timko MP

    更新日期:2001-10-23 00:00:00

  • Structure and backbone dynamics of Apo-CBFbeta in solution.

    abstract::Runx proteins constitute a family of mammalian transcription factors that interact with DNA through their evolutionarily conserved Runt domain. CBFbeta, alternatively denoted PEBP2beta, is the non-DNA-binding heterodimer partner and acts to enhance the DNA binding affinity of Runx proteins. Runx proteins and CBFbeta a...


    pub_type: 杂志文章


    authors: Wolf-Watz M,Grundström T,Härd T

    更新日期:2001-09-25 00:00:00

  • A fluorescent hydrophobic probe used for monitoring the kinetics of exocytosis phenomena.

    abstract::A fluorescence method is presented for quantitatively analyzing exocytosis phenomena and monitoring their kinetics. The method is based on the particular properties of a hydrophobic fluorescent probe, 1-[4-(trimethylammonio)phenyl]-6-phenylhexa-1,3,5-triene (TMA-DPH) [Prendergast, F.G., Haugland, R.P., & Callahan, P.J...


    pub_type: 杂志文章


    authors: Bronner C,Landry Y,Fonteneau P,Kuhry JG

    更新日期:1986-04-22 00:00:00

  • Substrate specificity of the heparan sulfate hexuronic acid 2-O-sulfotransferase.

    abstract::The interaction of heparan sulfate with different ligand proteins depends on the precise location of O-sulfate groups in the polysaccharide chain. We have previously shown that overexpression in human kidney 293 cells of a mouse mastocytoma 2-O-sulfotransferase (2-OST), previously thought to catalyze the transfer of s...


    pub_type: 杂志文章


    authors: Rong J,Habuchi H,Kimata K,Lindahl U,Kusche-Gullberg M

    更新日期:2001-05-08 00:00:00

  • Characterization of a mutant Bacillus subtilis adenylosuccinate lyase equivalent to a mutant enzyme found in human adenylosuccinate lyase deficiency: asparagine 276 plays an important structural role.

    abstract::Adenylosuccinate lyase, an enzyme catalyzing two reactions in purine biosynthesis (the cleavage of either adenylosuccinate or succinylaminoimidazole carboxamide ribotide), has been implicated in a human disease arising from point mutations in the gene encoding the enzyme. Asn(276) of Bacillus subtilis adenylosuccinate...


    pub_type: 杂志文章


    authors: Palenchar JB,Colman RF

    更新日期:2003-02-25 00:00:00

  • Increased Turnover at Limiting O2 Concentrations by the Thr(387) → Ala Variant of HIF-Prolyl Hydroxylase PHD2.

    abstract::PHD2 is a 2-oxoglutarate, non-heme Fe(2+)-dependent oxygenase that senses O2 levels in human cells by hydroxylating two prolyl residues in the oxygen-dependent degradation domain (ODD) of HIF1α. Identifying the active site contacts that determine the rate of reaction at limiting O2 concentrations is crucial for unders...


    pub_type: 杂志文章


    authors: Pektas S,Taabazuing CY,Knapp MJ

    更新日期:2015-05-12 00:00:00

  • Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant.

    abstract::Histidine-40 is known to participate in phosphodiester transesterification catalyzed by the enzyme ribonuclease T1. A mutant enzyme with a lysine replacing the histidine-40 (His40Lys RNase T1) retains considerable catalytic activity [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-90...


    pub_type: 杂志文章


    authors: Zegers I,Verhelst P,Choe HW,Steyaert J,Heinemann U,Saenger W,Wyns L

    更新日期:1992-11-24 00:00:00

  • Primary and secondary photodynamics of the violet/orange dual-cysteine NpF2164g3 cyanobacteriochrome domain from Nostoc punctiforme.

    abstract::Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Like phytochromes, CBCRs photointerconvert between two photostates that accompany photoisomerization of their bilin chromophores. While phytochromes typically exhibit red/far-red photocycles, CBCR photocycles are much mor...


    pub_type: 杂志文章


    authors: Gottlieb SM,Kim PW,Corley SC,Madsen D,Hanke SJ,Chang CW,Rockwell NC,Martin SS,Lagarias JC,Larsen DS

    更新日期:2014-02-18 00:00:00

  • A hydrophobic inhibitor of the nicotinic acetylcholine receptor acts on the resting state.

    abstract::3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...


    pub_type: 杂志文章


    authors: Wu G,Raines DE,Miller KW

    更新日期:1994-12-27 00:00:00

  • Long-range structural and dynamical changes induced by cofactor binding in DNA methyltransferase M.HhaI.

    abstract::The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...


    pub_type: 杂志文章


    authors: Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

    更新日期:2007-06-19 00:00:00

  • Simulation-based prediction of phosphatidylinositol 4,5-bisphosphate binding to an ion channel.

    abstract::Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...


    pub_type: 杂志文章


    authors: Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

    更新日期:2013-01-15 00:00:00

  • Preferential assembly of the tropomyosin heterodimer: equilibrium studies.

    abstract::Thermal unfolding/refolding studies of the three tropomyosin dimers, alpha alpha, alpha beta, and beta beta, from chicken gizzard muscle were performed to explain the preferential assembly of alpha- and beta-tropomyosin subunits into heterodimers, alpha beta [Lehrer, S. S., & Qian, Y. (1989) J. Biol. Chem. 265, 1134]....


    pub_type: 杂志文章


    authors: Lehrer SS,Stafford WF 3rd

    更新日期:1991-06-11 00:00:00

  • Evidence for a quantitative tissue-specific distribution of high mobility group chromosomal proteins.

    abstract::The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...


    pub_type: 杂志文章


    authors: Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

    更新日期:1980-09-16 00:00:00

  • hsp70 interacting protein Hip does not affect glucocorticoid receptor folding by the hsp90-based chaperone machinery except to oppose the effect of BAG-1.

    abstract::Reticulocyte lysate contains a chaperone system that assembles glucocorticoid receptor (GR).hsp90 heterocomplexes. Using purified proteins, we have prepared a five-protein heterocomplex assembly system consisting of two proteins essential for heterocomplex assembly-hsp90 and hsp70-and three proteins that act as co-cha...


    pub_type: 杂志文章


    authors: Kanelakis KC,Murphy PJ,Galigniana MD,Morishima Y,Takayama S,Reed JC,Toft DO,Pratt WB

    更新日期:2000-11-21 00:00:00

  • Additive transfer free energies of the peptide backbone unit that are independent of the model compound and the choice of concentration scale.

    abstract::With knowledge of individual transfer free energies of chemical groups that become newly exposed on protein denaturation and assuming the group transfer free energy contributions are additive, it should be possible to predict the stability of a protein in the presence of denaturant. Unfortunately, several unresolved i...


    pub_type: 杂志文章


    authors: Auton M,Bolen DW

    更新日期:2004-02-10 00:00:00

  • Inhibitory copper binding site on the spinach cytochrome b6f complex: implications for Qo site catalysis.

    abstract::The isolated cytochrome (cyt) b(6)f complex from spinach is inhibited by Cu(2+) with a K(D) of about 1 microM at pH 7.6 in the presence of 1.6 microM decyl-plastoquinol (C(10)-PQH(2)) as a substrate. Inhibition was competitive with respect to C(10)-PQH(2) but noncompetitive with respect to horse heart cyt c or plastoc...


    pub_type: 杂志文章


    authors: Rao BK,Tyryshkin AM,Roberts AG,Bowman MK,Kramer DM

    更新日期:2000-03-28 00:00:00

  • Spin equilibrium in human methemoglobin: effects of inositol hexaphosphate and bezafibrate as measured by resonance Raman spectroscopy.

    abstract::The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...


    pub_type: 杂志文章


    authors: Noble RW,DeYoung A,Rousseau DL

    更新日期:1989-06-13 00:00:00

  • Hemorrhagic toxins from Western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and the role of zinc in hemorrhagic toxin e.

    abstract::Five previously unknown hemorrhagic proteins, designated hemorrhagic toxins a,b,c,d, and e, were isolated from the venom of the western diamondback rattlesnake (Crotalus atrox). Molecular weights of hemorrhagic toxins a-e were determined to be 68 000, 24 000, 24 000, 24 000, and 25 700, respectively, by sodium dodecyl...


    pub_type: 杂志文章


    authors: Bjarnason JB,Tu AT

    更新日期:1978-08-08 00:00:00

  • Human lysosomal sphingomyelinase: substrate efficacy of apolipoprotein/sphingomyelin complexes.

    abstract::Human apolipoprotein stimulation of sphingomyelin (SM) hydrolysis by sphingomyelinase from human skin fibroblasts has been studied. Apolipoproteins A-I, A-II, B, C-I, and E do not enhance sphingomyelin hydrolysis above control levels. In contrast, apoC-II stimulates sphingomyelin hydrolysis by approximately 2.5-fold. ...


    pub_type: 杂志文章


    authors: Ahmad TY,Beaudet AL,Sparrow JT,Morrisett JD

    更新日期:1986-07-29 00:00:00

  • Biosynthesis of lipid A in Escherichia coli: identification of UDP-3-O-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine as a precursor of UDP-N2,O3-bis[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine.

    abstract::The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., ...


    pub_type: 杂志文章


    authors: Anderson MS,Robertson AD,Macher I,Raetz CR

    更新日期:1988-03-22 00:00:00

  • pH-dependent spectral and kinetic properties of cytochrome c peroxidase: comparison of freshly isolated and stored enzyme.

    abstract::The effect of long-term storage on the electronic absorption spectrum and the kinetic properties of cytochrome c peroxidase has been investigated. No detectable differences were observed between freshly isolated enzyme and enzyme stored below -20 degrees C, in the crystalline state, for up to 41 months. The electronic...


    pub_type: 杂志文章


    authors: Vitello LB,Huang M,Erman JE

    更新日期:1990-05-08 00:00:00

  • Lipid interaction converts prion protein to a PrPSc-like proteinase K-resistant conformation under physiological conditions.

    abstract::The conversion of prion protein (PrP) to the pathogenic PrPSc conformation is central to prion disease. Previous studies revealed that PrP interacts with lipids and the interaction induces PrP conformational changes, yet it remains unclear whether in the absence of any denaturing treatment, PrP-lipid interaction is su...


    pub_type: 杂志文章


    authors: Wang F,Yang F,Hu Y,Wang X,Wang X,Jin C,Ma J

    更新日期:2007-06-12 00:00:00

  • Cross-linking of a monomeric 39/34-kDa dispase fragment of von Willebrand factor (Leu-480/Val-481-Gly-718) to the N-terminal region of the alpha-chain of membrane glycoprotein Ib on intact platelets with bis(sulfosuccinimidyl) suberate.

    abstract::A 39/34-kilodalton (kDa) monomeric dispase fragment of von Willebrand factor (vWF) has been purified by heparin affinity chromatography. Detailed structural analysis of the individual 39- and 34-kDa fragments indicated that they had identical amino acid sequences extending from Leu-480/Val-481 to Gly-718 with an intra...


    pub_type: 杂志文章


    authors: Andrews RK,Gorman JJ,Booth WJ,Corino GL,Castaldi PA,Berndt MC

    更新日期:1989-10-17 00:00:00