Abnormal solubility behavior of beta-lactoglobulin: salting-in by glycine and NaCl.

abstract：:MAP kinase kinase (MAPKK) is a dual specificity protein kinase that phosphorylates and activates MAP kinase in vivo. In this study, four mouse monoclonal single-chain Fv (scFv) antibodies (Y1-6, Y1-7, Y3-6, and Y3-11) that can specifically bind to Xenopus MAPKK were isolated from combinatorial scFv-displaying phage li...

journal_title：Biochemistry

authors： Kosako H,Akamatsu Y,Tsurushita N,Lee KK,Gotoh Y,Nishida E

更新日期：1996-10-08 00:00:00

abstract：:The interaction of the retroviral nucleocapsid protein (NC) with nucleic acids forms the basis of its varied roles in the replication cycle, which include binding and condensing the viral RNA within the virion, stimulation of the early steps in reverse transcription, and dissociation from RNA in the replication comple...

journal_title：Biochemistry

authors： Secnik J,Gelfand CA,Jentoft JE

更新日期：1992-03-24 00:00:00

abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...

journal_title：Biochemistry

authors： Pedemonte CH,Kaplan JH

更新日期：1992-11-03 00:00:00

abstract：:The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia intermediate does not dissociate into solution during the chemical transformations. A well-characterized example of the structure and mechani...

journal_title：Biochemistry

authors： Raushel FM,Thoden JB,Holden HM

更新日期：1999-06-22 00:00:00

abstract：:The aminoacylation kinetics of 19 different variants of yeast tRNATyr with nucleotide substitutions in positions 33-35 were determined. Substitution of the conserved uridine-33 does not alter the rate of aminoacylation. However, substitution of the anticodon position 34 or position 35 reduces Km from 2- to 10-fold and...

journal_title：Biochemistry

authors： Bare LA,Uhlenbeck OC

更新日期：1986-09-23 00:00:00

abstract：:IFABP is a small beta-barrel protein with a short helix-turn-helix motif near the N-terminus that is thought to participate in the regulation of the uptake and delivery of fatty acids. In a previous work, we detected by near UV circular dichroism a reversible conformational transition of this protein occurring between...

journal_title：Biochemistry

authors： Arighi CN,Rossi JP,Delfino JM

更新日期：2003-06-24 00:00:00

abstract：:S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...

journal_title：Biochemistry

authors： Bale S,Brooks W,Hanes JW,Mahesan AM,Guida WC,Ealick SE

更新日期：2009-07-14 00:00:00

abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

journal_title：Biochemistry

authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

更新日期：2020-04-21 00:00:00

abstract：:Apolipoprotein E is immunochemically localized to amyloid plaque in Alzheimer's brains, and the allelic distribution of ApoE in individuals is associated with a disposition toward Alzheimer's disease. We show here that all three ApoE isotypes exhibit a strong and specific ability to inhibit both nucleation and seeding...

journal_title：Biochemistry

authors： Wood SJ,Chan W,Wetzel R

更新日期：1996-09-24 00:00:00

abstract：:We have characterized a membrane protein containing residues P688-T762 of the integrin beta3 subunit, encompassing its transmembrane and cytoplasmic domains, by nuclear magnetic resonance spectroscopy. Under conditions in which it is monomeric in dodecylphosphocholine micelles, the protein consists mainly of alpha-hel...

journal_title：Biochemistry

authors： Li R,Babu CR,Valentine K,Lear JD,Wand AJ,Bennett JS,DeGrado WF

更新日期：2002-12-31 00:00:00

abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...

journal_title：Biochemistry

authors： Askerka M,Vinyard DJ,Brudvig GW,Batista VS

更新日期：2015-09-29 00:00:00

abstract：:A homogeneous preparation of transketolase was obtained from spinach leaf; the specific enzyme activity was 9.5 mumolo of glyceraldehyde-3-P formed (mg of protein)-1 min-1, when xylulose-5-P and ribose-5-P were used as the donor and acceptor, respectively, of the ketol residue. Transketolase catalyzed the formation of...

journal_title：Biochemistry

authors： Takabe T,Asami S,Akazawa T

更新日期：1980-08-19 00:00:00

abstract：:S100A1, a member of the S100 protein family, is an EF-hand containing Ca(2+)-binding protein (93 residues per subunit) with noncovalent interactions at its dimer interface. Each subunit of S100A1 has four alpha-helices and a small antiparallel beta-sheet consistent with two helix-loop-helix calcium-binding domains [Ba...

journal_title：Biochemistry

authors： Rustandi RR,Baldisseri DM,Inman KG,Nizner P,Hamilton SM,Landar A,Landar A,Zimmer DB,Weber DJ

更新日期：2002-01-22 00:00:00

abstract：:Association of the human papillomavirus (HPV) E2 protein with its palindromic DNA-binding site is a necessary step for transcriptional trans-activation. To study the interaction between DNA and E2, the carboxyl-terminal domain of HPV-11 E2 protein (E2C) was expressed in Escherichia coli and purified to homogeneity. Th...

journal_title：Biochemistry

authors： Alexander KA,Phelps WC

更新日期：1996-07-30 00:00:00

abstract：:The human translin protein binds a variety of sequences (chromosomal breakpoint consensus sequences, their sequence variants, as well as nonbreakpoint sequences such as simple AT and GC repeats) at nanomolar protein concentration when short single strands ( approximately 20-30mers) are used as DNA targets. The protein...

journal_title：Biochemistry

authors： Sengupta K,Rao BJ

更新日期：2002-12-24 00:00:00

abstract：:A 16-mer deoxyribonucleotide was labeled at the 5'-end with x-rhodamine and at the 3'-end with fluorescein. Förster resonance energy transfer was used to determine the distribution, P(R), of donor-acceptor distances for the oligomer in three duplex structures (hybridized to complementary strands having 10, 16, and 24 ...

journal_title：Biochemistry

authors： Parkhurst KM,Parkhurst LJ

更新日期：1995-01-10 00:00:00

abstract：:Previously, we have demonstrated physical and functional interactions of the voltage-gated potassium channel Kv2.1 with the plasma membrane protein components of the exocytotic SNARE complex, syntaxin 1A, and the t-SNARE, syntaxin 1A/SNAP-25, complex. Importantly, the physical interaction of Kv2.1 with syntaxin was sh...

journal_title：Biochemistry

authors： Tsuk S,Lvov A,Michaelevski I,Chikvashvili D,Lotan I

更新日期：2008-08-12 00:00:00

abstract：:A human genomic DNA library was screened for the gene coding for the gamma chain of fibrinogen by using a human cDNA for the gamma chain as a hybridization probe. The gene was identified in three overlapping recombinant lambda bacteriophage, and its sequence, including the immediate 5' and 3' flanking regions, was det...

journal_title：Biochemistry

authors： Rixon MW,Chung DW,Davie EW

更新日期：1985-04-09 00:00:00

abstract：:Catalase (hydroperoxidase) HPII of Escherichia coli is the largest catalase so far characterized, existing as a homotetramer of 84 kDa subunits. Each subunit has a core structure that closely resembles small subunit catalases, supplemented with an extended N-terminal sequence and compact flavodoxin-like C-terminal dom...

journal_title：Biochemistry

authors： Chelikani P,Donald LJ,Duckworth HW,Loewen PC

更新日期：2003-05-20 00:00:00

abstract：:Isoform 1 of the mammalian Na(+)/H(+) exchanger (NHE1) is a ubiquitously expressed plasma membrane pH regulatory protein. It removes one intracellular H(+) in exchange for one extracellular Na(+). The 500 N-terminal amino acids comprise the catalytic membrane domain and fold into 12 transmembrane (TM) segments. To gai...

journal_title：Biochemistry

authors： Alves C,Lee BL,Sykes BD,Fliegel L

更新日期：2014-06-10 00:00:00

abstract：:The EcoRV DNA-(adenine-N6)-methyltransferase (MTase) recognizes GATATC sequences and modifies the first adenine residue within this site. Parts of its DNA interface show high sequence homology to DNA MTases of the dam family which recognize and modify GATC sequences. A phylogenetic analysis of M.EcoRV and dam-MTases s...

journal_title：Biochemistry

authors： Beck C,Cranz S,Solmaz M,Roth M,Jeltsch A

更新日期：2001-09-18 00:00:00

abstract：:H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the...

journal_title：Biochemistry

authors： Lasters I,Muyldermans S,Wyns L,Hamers R

更新日期：1981-03-03 00:00:00

abstract：:The structure of the subgel phase of dipalmitoylphosphatidylglycerol (DPPG) has been analyzed by X-ray diffraction techniques. Diffraction recorded from highly oriented DPPG specimens in the subgel phase extends to 2-A resolution. There are sharp lamellar reflections on the meridian, and other reflections lie on a ser...

journal_title：Biochemistry

authors： Blaurock AE,McIntosh TJ

更新日期：1986-01-28 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:The three-dimensional structure of toxic shock syndrome toxin 1 (TSST-1) from Staphylococcus aureus has been determined and refined to an R value of 0.226 for data between 8- and 2.5-A resolution. Overall, the structure of TSST-1 is similar to that of another superantigen, staphylococcal enterotoxin B (SEB). The key d...

journal_title：Biochemistry

authors： Prasad GS,Earhart CA,Murray DL,Novick RP,Schlievert PM,Ohlendorf DH

更新日期：1993-12-21 00:00:00

abstract：:A wide variety of biological processes including differentiation, regeneration, and cancer progression are regulated by shedding of membrane-anchored proteins. One of the major sheddases is A Disintegrin And Metalloprotease-17 (ADAM17) whose extracellular region consists of a pro-, a catalytic, a disintegrin-, and a m...

journal_title：Biochemistry

authors： Düsterhöft S,Michalek M,Kordowski F,Oldefest M,Sommer A,Röseler J,Reiss K,Grötzinger J,Lorenzen I

更新日期：2015-09-29 00:00:00

abstract：:The genomic hypervariation of human immunodeficiency virus 1 (HIV-1) could result from misincorporations by the viral reverse transcriptase. We developed an assay for reverse transcriptase fidelity during RNA-dependent as well as DNA-dependent DNA polymerization in vitro. A lacZ alpha RNA fragment transcribed by T3 RN...

journal_title：Biochemistry

authors： Ji JP,Loeb LA

更新日期：1992-02-04 00:00:00

abstract：:The skeletal muscle Ca2+ release channel (RYR1) is regulated by calmodulin in both its Ca2+-free (apocalmodulin) and Ca2+-bound (Ca2+ calmodulin) states. Apocalmodulin is an activator of the channel, and Ca2+ calmodulin is an inhibitor of the channel. Both apocalmodulin and Ca2+ calmodulin binding sites on RYR1 are de...

journal_title：Biochemistry

authors： Moore CP,Rodney G,Zhang JZ,Santacruz-Toloza L,Strasburg G,Hamilton SL

更新日期：1999-06-29 00:00:00

abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

journal_title：Biochemistry

authors： Beard WA,Wilson SH

更新日期：2014-05-06 00:00:00

abstract：:Regulation of adenylate cyclase by alpha 2 adrenergic receptors requires the inhibitory guanine nucleotide binding protein Ni. A role for this protein has also been suggested in the high-affinity binding of agonists to the alpha 2 receptor. We recently reported that alkaline treatment can selectively inactivate alpha ...

journal_title：Biochemistry

authors： Kim MH,Neubig RR

更新日期：1987-06-16 00:00:00