Structure of toxic shock syndrome toxin 1.

abstract：:A hairpin structure contains two conformationally distinct domains: a double-helical stem with Watson-Crick base pairs and a single-stranded loop that connects the two arms of the stem. By extensive 1D and 2D 500-MHz 1H NMR studies in H2O and D2O, it has been demonstrated that the DNA oligomers d(CGCCGCAGC) and d(CGCC...

journal_title：Biochemistry

authors： Gupta G,Sarma MH,Sarma RH,Bald R,Engelke U,Oei SL,Gessner R,Erdmann VA

更新日期：1987-12-01 00:00:00

abstract：:Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...

journal_title：Biochemistry

authors： Wang L,Tharp S,Selzer T,Benkovic SJ,Kohen A

更新日期：2006-02-07 00:00:00

abstract：:Electron nuclear double resonance (ENDOR) of protons at Type 2 and Type 1 cupric active sites correlates with the enzymatic pH dependence, the mutation of nearby conserved, nonligating residues, and electron transfer in heterologously expressed Rhodobacter sphaeroides nitrite reductase. Wild-type enzyme showed a pH 6 ...

journal_title：Biochemistry

authors： Zhao Y,Lukoyanov DA,Toropov YV,Wu K,Shapleigh JP,Scholes CP

更新日期：2002-06-11 00:00:00

abstract：:We have designed a novel estrogen-responsive unit, overERE, which consists of two overlapping ERE separated by 5 bp (center-to-center). In gel retardation assays, this sequence forms a low-mobility complex that migrates like an estrogen receptor tetramer. The receptor-overERE complex was specific and was supershifted ...

journal_title：Biochemistry

authors： Massaad C,Coumoul X,Sabbah M,Garlatti M,Redeuilh G,Barouki R

更新日期：1998-04-28 00:00:00

abstract：:Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...

journal_title：Biochemistry

authors： Ohanian V,Wolfe LC,John KM,Pinder JC,Lux SE,Gratzer WB

更新日期：1984-09-11 00:00:00

abstract：:The folding behavior of bovine growth hormone (bGH) is examined by chemical and pH denaturation using several spectroscopic probes of protein secondary and tertiary structure. Partially denaturing concentrations of urea eliminate the native-state quenching of intrinsic tryptophan fluorescence, from the single protein ...

journal_title：Biochemistry

authors： Holzman TF,Dougherty JJ Jr,Brems DN,MacKenzie NE

更新日期：1990-02-06 00:00:00

abstract：:In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond (

journal_title：Biochemistry

authors： Shi X,Abbyad P,Shu X,Kallio K,Kanchanawong P,Childs W,Remington SJ,Boxer SG

更新日期：2007-10-30 00:00:00

abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...

journal_title：Biochemistry

authors： Yang L,Sun MF,Gailani D,Rezaie AR

更新日期：2009-02-24 00:00:00

abstract：:In mammals, citric acid cycle intermediates play a key role in regulating various metabolic processes, such as fatty acid synthesis and glycolysis. Members of the sodium-dependent SLC13 transporter family mediate the transport of di- and tricarboxylates into cells. SLC13 family members have been implicated in lifespan...

journal_title：Biochemistry

authors： Colas C,Pajor AM,Schlessinger A

更新日期：2015-08-11 00:00:00

abstract：:Activation enthalpies and entropies are reported for proton-deuteron exchange at 42 amide sites in T4 lysozyme and compared with activation volumes for the same residues obtained earlier [Hitchens, T. K., and Bryant, R. G. (1998) Biochemistry 37, 5878-5887]. There is no correlation found between activation volume and ...

journal_title：Biochemistry

authors： Dixon ME,Hitchens TK,Bryant RG

更新日期：2000-01-11 00:00:00

abstract：:Mutation of Pro94 to phenylalanine or alanine significantly alters the redox properties of the type I copper center of amicyanin. Each mutation increases the redox midpoint potential (E(m)) value by at least 140 mV and shifts the pK(a) for the pH dependence of the E(m) value to a more acidic value. Atomic resolution (...

journal_title：Biochemistry

authors： Carrell CJ,Sun D,Jiang S,Davidson VL,Mathews FS

更新日期：2004-07-27 00:00:00

abstract：:Botulinum neurotoxins (BoNTs) elicit flaccid paralysis through cleavage of SNARE proteins within peripheral neurons. There are seven serotypes of the BoNTs, termed A-G, which differ in the SNARE protein and/or site that is cleaved. BoNTs are single-chain toxins that comprise an N-terminal zinc metalloprotease domain t...

journal_title：Biochemistry

authors： Baldwin MR,Barbieri JT

更新日期：2007-03-20 00:00:00

abstract：:A major regulatory step in the heat-induced transcription of heat shock protein (hsp) genes in eukaryotes is the activation of heat shock factor (HSF). In metazoans and Schizosaccharomyces pombe, HSF is present in unstressed cells but is unable to bind to its target DNA sequence element, the heat shock element (HSE). ...

journal_title：Biochemistry

authors： Larson JS,Schuetz TJ,Kingston RE

更新日期：1995-02-14 00:00:00

abstract：:The interaction of novel, tetrafunctional bisplatinum compounds with DNA was investigated. These compounds have bis(vicinal 1,2-diamines) as ligand. The reactions' efficiency, types of cross-links, alterations of the global DNA structure, and sequence selectivity differ significantly from the corresponding features of...

journal_title：Biochemistry

authors： Büning H,Altman J,Beck W,Zorbas H

更新日期：1997-09-23 00:00:00

abstract：:Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...

journal_title：Biochemistry

authors： Dancheck B,Nairn AC,Peti W

更新日期：2008-11-25 00:00:00

abstract：:Alpha-synuclein modulates dopamine homeostasis in dopamine-producing neurons of substantia nigra, partly through regulation of human dopamine transporter (hDAT) activity. To identify the underlying mechanisms, we disrupted the modulation of hDAT activity by wild-type (wt) alpha-synuclein, and its familial Parkinson's ...

journal_title：Biochemistry

authors： Wersinger C,Vernier P,Sidhu A

更新日期：2004-02-10 00:00:00

abstract：:The search for a rational method of developing an antimalarial vaccine (malaria caused by Plasmodium falciparum) consists of blocking receptor-ligand interaction. Conserved peptides derived from proteins involved in invasion and having strong red blood cell binding ability have thus been identified; immunization studi...

journal_title：Biochemistry

authors： Patarroyo ME,Alba MP,Vargas LE,Silva Y,Rosas J,Rodríguez R

更新日期：2005-05-10 00:00:00

abstract：:Photosystem II, the multisubunit protein complex that oxidizes water to O2, requires the inorganic cofactors Ca2+ and Cl- to exhibit optimal activity. Chloride can be replaced functionally by a small number of anionic cofactors (Br-, NO3-, NO2-, I-), but among these anions, only Br- is capable of restoring rates of ox...

journal_title：Biochemistry

authors： Wincencjusz H,Yocum CF,van Gorkom HJ

更新日期：1999-03-23 00:00:00

abstract：:Alzheimer's disease (AD) paired helical filaments (PHFs), building blocks of neurofibrillary tangles (NFTs) are composed of hyperphosphorylated forms of the microtubule-associated protein tau (i.e., PHF-tau). Currently, much effort is devoted to the development of diagnostic antibodies specific for PHF-tau since eleva...

journal_title：Biochemistry

authors： Hoffmann R,Lee VM,Leight S,Varga I,Otvos L Jr

更新日期：1997-07-01 00:00:00

abstract：:The pH dependence of the 13C chemical shifts of the side-chain carboxyl carbons of all Asp and Glu residues in the reduced and oxidized states of human thioredoxin and in a mixed disulfide complex of human thioredoxin with a target peptide from the transcription factor NF kappa B has been investigated by multidimensio...

journal_title：Biochemistry

authors： Qin J,Clore GM,Gronenborn AM

更新日期：1996-01-09 00:00:00

abstract：:Vertical fluctuations of the terminal methyl groups of stearic acid acyl chains toward the surface of dimyristoylphosphatidylcholine (DMPC) bilayers have been investigated by using spin-label electron-electron double-resonance ( ELDOR ) methodology. Spin-label pairs consisting of two populations of stearic acid spin-l...

journal_title：Biochemistry

authors： Feix JB,Popp CA,Venkataramu SD,Beth AH,Park JH,Hyde JS

更新日期：1984-05-08 00:00:00

abstract：:CNAD (5-beta-D-ribofuranosylnicotinamide adenine dinucleotide) is an isosteric C-glycosidic analogue of NAD(H) containing a neutral pyridine ring. CPAD (5-beta-D-ribofuranosylpicolinamide adenine dinucleotide) is a closely related pyridine-containing analogue with the pyridine nitrogen on the opposite side of the ring...

journal_title：Biochemistry

authors： Li H,Hallows WH,Punzi JS,Pankiewicz KW,Watanabe KA,Goldstein BM

更新日期：1994-10-04 00:00:00

abstract：:The major excreted protein (MEP) of transformed mouse fibroblasts has been purified, and monospecific antisera against it have been prepared. Synthesis and secretion of this protein have previously been shown to be stimulated by transformation or treatment with tumor-promoting phorbol esters, but its function is still...

journal_title：Biochemistry

authors： Gottesman MM,Cabral F

更新日期：1981-03-17 00:00:00

abstract：:The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...

journal_title：Biochemistry

authors： Darfeuille F,Arzumanov A,Gait MJ,Di Primo C,Toulmé JJ

更新日期：2002-10-08 00:00:00

abstract：:Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter gene transactivation by p53 was determined in yeast lacking the TRX1 and TRX2 genes encoding cytosolic...

journal_title：Biochemistry

authors： Stoner CS,Pearson GD,Koç A,Merwin JR,Lopez NI,Merrill GF

更新日期：2009-09-29 00:00:00

abstract：:Mutations in human copper-zinc superoxide dismutase (SOD1) cause an inherited form of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS). Here, we present structures of the pathogenic SOD1 variants D124V and H80R, both of which demonstrate compromised zinc-binding sites. The disruption of the zinc...

journal_title：Biochemistry

authors： Seetharaman SV,Winkler DD,Taylor AB,Cao X,Whitson LJ,Doucette PA,Valentine JS,Schirf V,Demeler B,Carroll MC,Culotta VC,Hart PJ

更新日期：2010-07-13 00:00:00

abstract：:We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...

journal_title：Biochemistry

authors： Erie DA,Jones RA,Olson WK,Sinha NK,Breslauer KJ

更新日期：1989-01-10 00:00:00

abstract：:The derivatives of calf thymus DNA in which n-butylamine is covalently attached as described in the preceding paper in this series [Chen, C. Y., Pheiffer, B. H., Zimmerman, S. B., & Hanlon, S. (1983) Biochemistry (preceding paper in this issue)] were examined by Raman spectroscopy. As previously mentioned, these compl...

journal_title：Biochemistry

authors： Fish SR,Chen CY,Thomas GJ Jr,Hanlon S

更新日期：1983-09-27 00:00:00

abstract：:Here we demonstrate that monovalent cations can localize around B-DNA in geometrically regular, sequence-specific sites in oligonucleotide crystals. Positions of monovalent ions were determined from high-resolution X-ray diffraction of DNA crystals grown in the presence of thallium(I) cations (Tl(+)). Tl(+) has previo...

journal_title：Biochemistry

authors： Howerton SB,Sines CC,VanDerveer D,Williams LD

更新日期：2001-08-28 00:00:00

abstract：:The complete coordination scheme for Mn(II) in transition-state-analogue complexes with creatine kinase has been determined by electron paramagnetic resonance (EPR) spectroscopy. Perturbations in the EPR spectra for Mn(II) due to superhyperfine coupling to 17O of selectively labeled ligands have been used to identify ...

journal_title：Biochemistry

authors： Reed GH,Leyh TS

更新日期：1980-11-25 00:00:00