Characterization of the monomeric form of the transmembrane and cytoplasmic domains of the integrin beta 3 subunit by NMR spectroscopy.

abstract：:The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...

journal_title：Biochemistry

authors： Holowka DA,Cathou RE

更新日期：1976-07-27 00:00:00

abstract：:Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...

journal_title：Biochemistry

authors： Dolence JM,Steward LE,Dolence EK,Wong DH,Poulter CD

更新日期：2000-04-11 00:00:00

abstract：:Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent on the presence of negatively charged lipids. By using a phospholipid with an acyl chain linked photoactivatable group,...

journal_title：Biochemistry

authors： van Voorst F,van der Does C,Brunner J,Driessen AJ,de Kruijff B

更新日期：1998-09-01 00:00:00

abstract：:Oligonucleotides capable of sequence-specific triple helix formation have been proposed as DNA binding ligands useful for modulation of gene expression and for directed genome modification. However, the effectiveness of such triplex-forming oligonucleotides (TFOs) depends on their ability to bind to their target sites...

journal_title：Biochemistry

authors： Lacroix L,Lacoste J,Reddoch JF,Mergny JL,Levy DD,Seidman MM,Matteucci MD,Glazer PM

更新日期：1999-02-09 00:00:00

abstract：:The reaction of Pseudomonas fluorescens kynureninase with L-kynurenine and L-alanine has been examined using rapid-scanning stopped-flow spectrophotometry. Mixing kynureninase with 0.5 mM L-kynurenine results in formation of a quinonoid intermediate, with lambdamax = 494 nm, within the dead time (ca. 2 ms) of the stop...

journal_title：Biochemistry

authors： Phillips RS,Sundararaju B,Koushik SV

更新日期：1998-06-16 00:00:00

abstract：:The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...

journal_title：Biochemistry

authors： Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

更新日期：1992-12-08 00:00:00

abstract：:The guanyl nucleotide binding regulatory protein of retinal rod outer segments, called Gt, that couples the photon receptor rhodopsin with the light-activated cGMP phosphodiesterase, can be resolved into two functional components, alpha t and beta gamma t. The effect of monoclonal antibody binding to the alpha t subun...

journal_title：Biochemistry

authors： Mazzoni MR,Hamm HE

更新日期：1989-12-12 00:00:00

abstract：:The dimeric two-component system transmitter protein NRII (NtrB) of Escherichia coli, product of glnL (ntrB), controls transcription of nitrogen-regulated genes by catalyzing the phosphorylation and dephosphorylation of the transcription factor NRI (NtrC). Previous studies showed that the PII signal transduction prote...

journal_title：Biochemistry

authors： Jiang P,Atkinson MR,Srisawat C,Sun Q,Ninfa AJ

更新日期：2000-11-07 00:00:00

abstract：:Myeloperoxidase (MPO) structural analysis has suggested that halides and pseudohalides bind to the distal binding site and serve as substrates or inhibitors, while others have concluded that there are two separate sites. Here, evidence for two distinct binding sites for halides comes from the bell-shaped effects obser...

journal_title：Biochemistry

authors： Proteasa G,Tahboub YR,Galijasevic S,Raushel FM,Abu-Soud HM

更新日期：2007-01-16 00:00:00

abstract：:Solution scattering studies were performed on a ligand-binding domain (S1S2) of a glutamate receptor ion channel (GluR) in order to study GluR-binding and signal-transduction mechanisms. The core of the ligand-binding domain is homologous to prokaryotic periplasmic binding proteins (PBP), whose binding mechanism invol...

journal_title：Biochemistry

authors： Abele R,Svergun D,Keinänen K,Koch MH,Madden DR

更新日期：1999-08-24 00:00:00

abstract：:Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...

journal_title：Biochemistry

authors： Sugiki T,Furuita K,Fujiwara T,Kojima C

更新日期：2018-07-03 00:00:00

abstract：:A covalent dimer of Saccharomyces cerevisiae iso-1 cytochrome c is stabilized by an interchain disulfide bond involving the cysteine residue penultimate to the C-terminus. The individual chains in the dimer appear to retain the tertiary structural features characteristic for monomeric cytochrome c albeit with some per...

journal_title：Biochemistry

authors： Bryant C,Strottmann JM,Stellwagen E

更新日期：1985-07-02 00:00:00

abstract：:The multimeric cytochromes described to date in sulfate- and sulfur-reducing bacteria are associated with diverse respiratory modes involving the use of elemental sulfur or oxidized sulfur compounds as terminal acceptors. They exhibit no structural similarity with the other cytochrome c classes and are characterized b...

journal_title：Biochemistry

authors： Bruschi M,Woudstra M,Guigliarelli B,Asso M,Lojou E,Petillot Y,Abergel C

更新日期：1997-09-02 00:00:00

abstract：:Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...

journal_title：Biochemistry

authors： Crute JJ,Wahl AF,Bambara RA

更新日期：1986-01-14 00:00:00

abstract：:The binding of ATP to pertussis toxin and its components, the A subunit and B oligomer, was investigated. Whereas, radiolabeled ATP bound to the B oligomer and pertussis toxin, no binding to the A subunit was observed. The binding of [3H]ATP to pertussis toxin and the B oligomer was inhibited by nucleotides. The relat...

journal_title：Biochemistry

authors： Hausman SZ,Manclark CR,Burns DL

更新日期：1990-07-03 00:00:00

abstract：:As part of a search for organic compounds that selectively target RNA, we found that specific diphenylfuran derivatives, which are related to compounds that bind to the DNA minor groove, bind very strongly to RNA in a manner very sensitive to the structure of the compounds. In extended development of the diphenylfuran...

journal_title：Biochemistry

authors： Li K,Davis TM,Bailly C,Kumar A,Boykin DW,Wilson WD

更新日期：2001-02-06 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:We systematically and quantitatively investigated the structure and thermodynamics of G-quadruplexes of RNAs and corresponding DNAs of the same sequences under molecular crowding conditions that mimic the high osmotic stress induced by the numerous molecules inside of living cells. Structural analyses demonstrated tha...

journal_title：Biochemistry

authors： Zhang DH,Fujimoto T,Saxena S,Yu HQ,Miyoshi D,Sugimoto N

更新日期：2010-06-01 00:00:00

abstract：:Accumulation of the cytotoxic 40- to 42-residue beta-amyloid peptide represents the primary pathological process in Alzheimer's disease (AD). BACE1 (beta-site APP cleaving enzyme 1) is responsible for the initial required step in the neuronal amyloidogenic processing of beta-amyloid precursor protein and is a major dr...

journal_title：Biochemistry

authors： Wang YS,Beyer BM,Senior MM,Wyss DF

更新日期：2005-12-20 00:00:00

abstract：:The linker for activation of T-cells (LAT) is a palmitoylated integral membrane adaptor protein that resides in lipid membrane rafts and contains nine consensus putative tyrosine phosphorylation sites, several of which have been shown to serve as SH2 binding sites. Upon T-cell antigen receptor (TCR/CD3) engagement, LA...

journal_title：Biochemistry

authors： Perez-Villar JJ,Whitney GS,Sitnick MT,Dunn RJ,Venkatesan S,O'Day K,Schieven GL,Lin TA,Kanner SB

更新日期：2002-08-27 00:00:00

abstract：:The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...

journal_title：Biochemistry

authors： Carberry SE,Friedland DE,Rhoads RE,Goss DJ

更新日期：1992-02-11 00:00:00

abstract：:The Iowa point mutation in apolipoprotein A-I (G26R) leads to a systemic amyloidosis condition, and the Milano mutation (R173C) is associated with hypoalphalipoproteinemia, a reduced plasma level of high-density lipoprotein. To probe the structural effects that lead to these outcomes, we used amide hydrogen-deuterium ...

journal_title：Biochemistry

authors： Chetty PS,Ohshiro M,Saito H,Dhanasekaran P,Lund-Katz S,Mayne L,Englander W,Phillips MC

更新日期：2012-11-06 00:00:00

abstract：:The dependence of enzymatic catalysis on diffusion rates in solution was examined with regard to high specific activity carbonic anhydrase (CA II) by varying the viscosity of the reaction medium with added glycerol, sucrose, and ficoll (a copolymer of sucrose and epichlorohydrin). Responses of the Michaelis-Menten par...

journal_title：Biochemistry

authors： Pocker Y,Janjić N

更新日期：1987-05-05 00:00:00

abstract：:The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding ...

journal_title：Biochemistry

authors： Chu R,Pei W,Takei J,Bai Y

更新日期：2002-06-25 00:00:00

abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...

journal_title：Biochemistry

authors： Li H,Wang HY,Kang S,Silverman RB,Poulos TL

更新日期：2016-07-05 00:00:00

abstract：:The calcium pump [sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA)] transports Ca(2+) from the cytosol to the SR lumen at the expense of ATP hydrolysis and proton countertransport, thus playing a central role in Ca(2+) homeostasis and muscle contractility. Proton countertransport via deprotonation of transport site r...

journal_title：Biochemistry

authors： Ramírez-Salinas GL,Espinoza-Fonseca LM

更新日期：2015-08-25 00:00:00

abstract：:Oxidation of the biological antioxidant alpha-tocopherol (vitamin E; TH) by peroxyl radicals yields 8a-(alkyldioxy)tocopherones, which either may hydrolyze to alpha-tocopheryl quinone (TQ) or may be reduced by ascorbic acid to regenerate TH. To define the chemistry of this putative two-electron TH redox cycle, we stud...

journal_title：Biochemistry

authors： Liebler DC,Kaysen KL,Kennedy TA

更新日期：1989-12-12 00:00:00

abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

journal_title：Biochemistry

authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

更新日期：2014-04-22 00:00:00

abstract：:The mutants Arg54-->Leu, Arg54-->Lys, Arg305-->Lys, Asp309-->Glu, and Trp317-->Phe, located at subsites 1 and 2 in glucoamylase from Aspergillus niger, provide insight into the importance of specific hydrogen bonds and hydrophobic interactions in substrate recognition, catalytic mechanism, and stability. As suggested ...

journal_title：Biochemistry

authors： Frandsen TP,Christensen T,Stoffer B,Lehmbeck J,Dupont C,Honzatko RB,Svensson B

更新日期：1995-08-15 00:00:00

abstract：:Dihydrofolate reductase (DHFR) is a critical enzyme in de novo purine and thymidylate biosynthesis. An RNA gel mobility shift assay was used to demonstrate a specific interaction between human recombinant DHFR protein and its corresponding DHFR mRNA. Incubation of DHFR protein with either its substrates, dihydrofolate...

journal_title：Biochemistry

authors： Chu E,Takimoto CH,Voeller D,Grem JL,Allegra CJ

更新日期：1993-05-11 00:00:00