Abstract：
:The linker for activation of T-cells (LAT) is a palmitoylated integral membrane adaptor protein that resides in lipid membrane rafts and contains nine consensus putative tyrosine phosphorylation sites, several of which have been shown to serve as SH2 binding sites. Upon T-cell antigen receptor (TCR/CD3) engagement, LAT is phosphorylated by protein tyrosine kinases (PTK) and binds to the adaptors Gads and Grb2, as well as to phospholipase Cgamma1 (PLCgamma1), thereby facilitating the recruitment of key signal transduction components to drive T-cell activation. The LAT tyrosine residues Y(132), Y(171), Y(191), and Y(226) have been shown previously to be critical for binding to Gads, Grb2, and PLCgamma1. In this report, we show by generation of LAT truncation mutants that the Syk-family kinase ZAP-70 and the Tec-family kinase Itk favor phosphorylation of carboxy-terminal tyrosines in LAT. By direct binding studies using purified recombinant proteins or phosphopeptides and by mutagenesis of individual tyrosines in LAT to phenylalanine residues, we demonstrate that Y(171) and potentially Y(226) are docking sites for the Vav guanine nucleotide exchange factor. Further, overexpression of a kinase-deficient mutant of Itk in T-cells reduced both the tyrosine phosphorylation of endogenous LAT and the recruitment of Vav to LAT complexes. These data indicate that kinases from distinct PTK families are likely responsible for LAT phosphorylation following T-cell activation and that Itk kinase activity promotes recruitment of Vav to LAT.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Perez-Villar JJ,Whitney GS,Sitnick MT,Dunn RJ,Venkatesan S,O'Day K,Schieven GL,Lin TA,Kanner SBdoi
10.1021/bi025554osubject
Has Abstractpub_date
2002-08-27 00:00:00pages
10732-40issue
34eissn
0006-2960issn
1520-4995pii
bi025554ojournal_volume
41pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972303k
更新日期：1998-02-24 00:00:00
abstract：:Vertical fluctuations of the terminal methyl groups of stearic acid acyl chains toward the surface of dimyristoylphosphatidylcholine (DMPC) bilayers have been investigated by using spin-label electron-electron double-resonance ( ELDOR ) methodology. Spin-label pairs consisting of two populations of stearic acid spin-l...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00305a032
更新日期：1984-05-08 00:00:00
abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00652a031
更新日期：1976-04-06 00:00:00
abstract：:The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a023
更新日期：1985-10-22 00:00:00
abstract：:Self-assembly of beta-sheet domains resulting in the formation of pathogenic, fibrillar protein aggregates (amyloids) is a characteristic feature of various medical disorders. These include neurodegenerative diseases, such as Alzheimer's, Huntington's, and Creutzfeldt-Jacob's. A significant problem in studying such ag...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990510+
更新日期：1999-06-29 00:00:00
abstract：:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972602+
更新日期：1998-09-15 00:00:00
abstract：:Two synthetic peptides corresponding to sequences in HIV-1LAI gp41, (aa558-595) and T20 (aa 643-678), are strong inhibitors of HIV-1 viral fusion, having EC50 values of 1 microgram/mL and 1 ng/mL, respectively. Previous work suggested that T21 forms a coiled-coil structure in PBS solution, while T20 is primarily nonhe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9606962
更新日期：1996-10-22 00:00:00
abstract：:Human platelet glycoproteins Ib alpha, Ib beta, V, and IX comprise an interrelated set of molecules (the Ib-V-IX system) that together form a surface adhesion receptor for the ligand, von Willebrand factor. To complete the primary structural characterization of the genes involved in this system, we have analyzed cosmi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00049a028
更新日期：1995-12-12 00:00:00
abstract：:cis-3-Chloroacrylic acid dehalogenase (cis-CaaD) from Pseudomonas pavonaceae 170 and a homologue from Corynebacterium glutamicum designated Cg10062 are 34% identical in sequence (54% similar). The former catalyzes a key step in a bacterial catabolic pathway for the nematocide 1,3-dichloropropene, whereas the latter ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4004414
更新日期：2013-06-18 00:00:00
abstract：:Two-dimensional homonuclear and heteronuclear NMR and minimized potential energy calculations have been combined to define the structure of the antitumor agent mitomycin C (MC) cross-linked to deoxyguanosines on adjacent base pairs in the d(T1-A2-C3-G4-T5-A6).d(T7-A8-C9-G10-T11-A12) duplex. The majority of the mitomyc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00463a032
更新日期：1990-03-20 00:00:00
abstract：:The subunit structure of Hsp80, the most abundant heat-shock protein of Neurospora crassa, was examined by chemical cross-linking of the purified protein in vitro. Resolution of glutaraldehyde-treated Hsp80 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE suggests that the native state of this pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963030g
更新日期：1997-08-19 00:00:00
abstract：:Twelve site-directed mutants of rat cytochrome P450 2B1 distributed over seven positions and four putative substrate recognition sites (SRS) were constructed and expressed in COS cells. Function was examined using androstenedione and testosterone as substrates. Substitutions at positions 303, 360, and 473 did not mark...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00180a040
更新日期：1994-04-12 00:00:00
abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00196a009
更新日期：1994-08-02 00:00:00
abstract：:Using our Escherichia coli expression system, we have produced five mutant recombinant (r) hemoglobins (Hbs): r Hb (alpha V96 W), r Hb Presbyterian (beta N108K), r Hb Yoshizuka (beta N108D), r Hb (alpha V96W, beta N108K), and r Hb (alpha V96W, beta N108D). These r Hbs allow us to investigate the effect on the structur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990286o
更新日期：1999-07-06 00:00:00
abstract：:The coordination complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)3(3+)], which promotes RNA cleavage upon photoactivation, has been shown to target specifically guanine-uracil (G-U) mismatches in double-helical regions of folded RNAs. Photoactivated cleavage by Rh(DIP)3(3+) has been examined on a se...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00139a001
更新日期：1992-06-23 00:00:00
abstract：:The site-specific chemical modification of NADH-quinone oxidoreductase (complex I) by various functional probes such as fluorophores and microbeads, without affecting the enzyme activity, may allow single-molecule analyses of putative dynamic conformational changes in the enzyme. In an attempt to address this challeng...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500205x
更新日期：2014-04-15 00:00:00
abstract：:As part of a high-throughput, structural proteomic project we have used NMR spectroscopy to determine the solution structure and ascertain the function of a previously unknown, conserved protein (MtH895) from the thermophilic archeon Methanobacterium thermoautotrophicum. Our findings indicate that MtH895 contains a ce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0115176
更新日期：2002-04-16 00:00:00
abstract：:The effect of Ca2+ on the interaction of bovine cardiac myosin subfragment 1 (S-1) with actin regulated by cardiac troponin-tropomyosin was evaluated. The ratios of actin to troponin and to tropomyosin were adjusted to optimize the Ca2+-dependent regulation of the steady-state actin-activated magnesium adenosinetripho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00352a010
更新日期：1986-02-25 00:00:00
abstract：:Administration of pregnenolone-16 alpha-carbonitrile (PCN) to adult female rats caused a 2-fold increase in total liver microsomal cytochrome P-450 along with 5-7-fold increases in four in vitro monooxygenase activities considered diagnostic for the major PCN-inducible cytochrome P-450 isozyme. However, upon administr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00387a022
更新日期：1987-06-30 00:00:00
abstract：:In Sphingomonas CHY-1, a single ring-hydroxylating dioxygenase is responsible for the initial attack of a range of polycyclic aromatic hydrocarbons (PAHs) composed of up to five rings. The components of this enzyme were separately purified and characterized. The oxygenase component (ht-PhnI) was shown to contain one R...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0611311
更新日期：2006-10-10 00:00:00
abstract：:Assignments have been made for 11 methyl, one Gln-C gamma, one Thr-C beta, and all six Tyr-C zeta carbon resonances of ribonuclease A. These partially serve to delineate the binding sites for Cu2+, Mn2+, phosphate, cytidine and its 2'-, 3'-, and 5'-phosphates (Cyd and Cyd-2'-P, -3'-P, and -5'-P), and one or a few urea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a020
更新日期：1984-07-17 00:00:00
abstract：:The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00161a051
更新日期：1992-11-24 00:00:00
abstract：:Light-induced activation of the LOV2-Jα domain of the photoreceptor phototropin from oat is believed to involve the detachment of the Jα helix from the central β-sheet and its subsequent unfolding. The dynamics of these conformational changes were monitored by time-resolved emission spectroscopy with 100 ns time resol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101413v
更新日期：2010-12-28 00:00:00
abstract：:Galactolipids are characteristic lipids of the photosynthetic membranes. They are highly enriched in the chloroplast and are present in photosystem structures. There are two major types of galactolipids, i.e., monogalactosyldiacylglycerol and digalactosyldiacylglycerol (DGDG) in chloroplastic membranes, which amount t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00028
更新日期：2020-03-03 00:00:00
abstract：:R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034643d
更新日期：2003-09-16 00:00:00
abstract：:Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00469a004
更新日期：1990-05-01 00:00:00
abstract：:Raman difference spectroscopy is used to probe the properties of a 36-nt RNA molecule, "D5", which lies at the heart of the catalytic apparatus in group II introns. For D5 that has all of its adenine residues labeled with (13)C and (15)N and utilizing Raman difference spectroscopy, we identify the conformationally sen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi902117w
更新日期：2010-04-27 00:00:00
abstract：:The primary subsite specificities of human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A alpha, and the protease from strain V-8 of Staphylococcus aureus have been mapped with a series of tripeptide thiobenzyl ester substrates of the general formu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00308a023
更新日期：1984-06-19 00:00:00
abstract：:In Gram-negative bacteria, the first step of lipid A biosynthesis is catalyzed by UDP-N-acetylglucosamine acyltransferase (LpxA) through the transfer of a R-3-hydroxyacyl chain from the acyl carrier protein (ACP) to the 3-hydroxyl group of UDP-GlcNAc. Previous studies suggest that LpxA is a critical determinant of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00720
更新日期：2015-09-29 00:00:00
abstract：:The sigmoidal dependence of activity on substrate concentration exhibited by the regulatory enzyme aspartate transcarbamoylase (ATCase) of Escherichia coli is generally attributed to a ligand-promoted change in the quaternary structure of the enzyme. Although a global conformational change in ATCase upon the binding o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00467a019
更新日期：1990-04-17 00:00:00
