Abstract：
:The proposal that microtubule dynamic instability results from stabilization of microtubule ends by tubulin-GDP-Pi subunits (where Pi is inorganic phosphate) [Melki et al. (1996) Biochemistry 35, 12038] was based on studies of GTP hydrolysis and microtubule assembly that showed that tubulin-GDP-Pi subunits can transiently accumulate at microtubule ends. There is no direct evidence that GDP-Pi-subunits can stabilize microtubules under conditions where dynamic instability is observed and this has been inferred from the observation that tubulin-GDP-BeFn subunits stabilize microtubules. To test if tubulin-GDP-Pi stabilizes microtubules we sought evidence for a synergism between the effect of Pi and BeFn. We found, however, that Pi antagonizes the effect of BeFn by displacing it from tubulin subunits. The alternate mechanism in which Pi inhibits BeFn stabilization of microtubules by displacing fluoride from beryllium was ruled out from the 9Be and 19F NMR spectra in the presence and absence of Pi. Further evidence that tubulin-GDP-BeFn is not an analogue of tubulin-GDP-Pi and that tubulin-GDP-Pi is not responsible for maintaining the growth phase in microtubules manifesting dynamic instability was provided by our observation that Pi did not decrease the disassembly rate under conditions where tubulin-GDP-Pi subunits are expected to have formed. Results showing that BeFn binds randomly to subunits in microtubules provided evidence that Pi dissociation from the tubulin-GDP-Pi intermediate formed during GTP hydrolysis occurs randomly rather than processively starting at the growing microtubule tip.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Caplow M,Shanks Jdoi
10.1021/bi972602+subject
Has Abstractpub_date
1998-09-15 00:00:00pages
12994-3002issue
37eissn
0006-2960issn
1520-4995pii
bi972602+journal_volume
37pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The structure of the specific phosphate binding loop (P-loop) of bovine protein tyrosine phosphatase (BPTP) is very similar to that present in high M(r) PTPases. Site-directed mutagenesis was used to explore the role of several conserved residues involved in forming the P-loop of BPTP. Thus, Ser-19 and Ser-43 were ind...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9605651
更新日期：1996-10-22 00:00:00
abstract：:Monomeric sarcosine oxidase is a flavoenzyme that catalyzes the oxidation of the methyl group in sarcosine (N-methylglycine). Rapid reaction kinetic studies under anaerobic conditions at pH 8.0 show that the enzyme forms a charge transfer Michaelis complex with sarcosine (E-FAD(ox).sarcosine) that exhibits an intense ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0600852
更新日期：2006-05-16 00:00:00
abstract：:To contribute to the understanding of membrane protein function upon application of pressure as relevant for understanding, for example, the physiology of deep sea organisms or for baroenzymological biotechnical processes, we investigated the influence of hydrostatic pressure on the activity of Na+,K+-ATPase enriched ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062235e
更新日期：2007-02-13 00:00:00
abstract：:Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0118005
更新日期：2002-02-26 00:00:00
abstract：:The functionality of the membrane-bound, ubiquinone-dependent pyruvate oxidase from the respiratory chain of Escherichia coli was reconstituted with a supported lipidic structure. The artificial structure was especially designed to allow the electrochemical control of the quinone pool through the lateral mobility of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002325y
更新日期：2001-02-06 00:00:00
abstract：:The p90 ribosomal S6 family of kinases (RSK) are potential drug targets, due to their involvement in cancer and other pathologies. There are currently only two known selective inhibitors of RSK, but the basis for selectivity is not known. One of these inhibitors is a naturally occurring kaempferol-α-L-diacetylrhamnosi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300620c
更新日期：2012-08-21 00:00:00
abstract：:Tyr71 is an invariant residue in all known sequences of tyrosine phenol-lyase (TPL). The substitution of Tyr71 in TPL by phenylalanine results in a mutant Y71F TPL with no detectable activity (greater than 3 x 10(5)-fold reduction) for beta-elimination of L-tyrosine. Y71F TPL can react with S-alkylcysteines, but these...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00038a023
更新日期：1995-09-26 00:00:00
abstract：:The enzyme steroid 5 alpha-reductase utilizes NADPH to reduce the double bonds of a variety of steroid substrates with generalized 3-oxo, delta 4,5 structures. One substrate for this membrane-bound enzyme is testosterone, whose reduction to dihydrotestosterone is required for the embryonic differentiation of the exter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00171a029
更新日期：1994-02-08 00:00:00
abstract：:Aminoacylation of tRNA by aminoacyl-tRNA synthetases is the essential reaction that matches protein amino acids with the trinucleotide sequences specified in mRNA. Direct electrostatic interactions made by tRNA synthetases with discriminating functional groups on the tRNA bases have long been known to determine aminoa...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/bi7014647
更新日期：2007-09-18 00:00:00
abstract：:Association of the human papillomavirus (HPV) E2 protein with its palindromic DNA-binding site is a necessary step for transcriptional trans-activation. To study the interaction between DNA and E2, the carboxyl-terminal domain of HPV-11 E2 protein (E2C) was expressed in Escherichia coli and purified to homogeneity. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960447d
更新日期：1996-07-30 00:00:00
abstract：:Alignment of more than 20 deduced sequences for mevalonate diphosphate decarboxylase (MDD) indicates that serines 34, 36, 120,121, 153, and 155 are invariant residues that map within a proposed interdomain active site cleft. To test possible active site roles for these invariant serines, each has been mutated to alani...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0484217
更新日期：2005-02-22 00:00:00
abstract：:Class I major histocompatibility complex (MHC) proteins present peptide antigens to T cells during the immune response against viruses. Peptides are loaded into newly synthesized class I heterodimers in the endoplasmic reticulum such that most or all cell surface class I molecules contain peptides derived from endogen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00192a020
更新日期：1994-07-05 00:00:00
abstract：:Eosinophil cationic protein (ECP) is a component of the eosinophil granule matrix. It shows marked toxicity against helminth parasites, bacteria single-stranded RNA viruses, and host epithelial cells. Secretion of human ECP is related to eosinophil-associated allergic, asthmatic, and inflammatory diseases. ECP belongs...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0264521
更新日期：2002-10-08 00:00:00
abstract：:The gamma-phosphoryl groups of two intermediates (M-ATP and M-ADP-P1) in the pathway of MgATP hydrolysis by myosin undergo extensive oxygen exchange with water. Actin activates the overall rate of hydrolysis at a rate-limiting step which follows these exchange reactions. Thus, actin, by decreasing the turnover time of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00620a022
更新日期：1977-01-11 00:00:00
abstract：:To shed more light on the molecular requirements for recognition of thyroid response elements (TREs) by thyroid receptors (TRs), we compared the specific aspects of DNA TRE recognition by different TR constructs. Using fluorescence anisotropy, we performed a detailed and hierarchical study of TR-TRE binding. This was ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901282s
更新日期：2010-02-09 00:00:00
abstract：:The fluorescence polarization intensities from fluorescent probes and the electron paramagnetic resonance spectra from spin probes, specifically modifying elements of a biological assembly such as myosin sulfhydryl 1 (SH1) in muscle fibers, are interpreted in terms of probe order parameters using a model-independent m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00116a029
更新日期：1992-01-14 00:00:00
abstract：:Melittin is a 26-residue peptide which undergoes high-affinity calcium-dependent binding by calmodulin [Barnette, M.S., Daly, R., & Weiss, B. (1983) Biochem. Pharmacol. 32, 2929; Comte, M., Maulet, Y., & Cox, J.A. (1983) Biochem. J. 209, 269; Anderson, S.R., & Malencik, D.A. (1986) Calcium Cell Funct. 6, 1]. The resul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00406a021
更新日期：1988-03-22 00:00:00
abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961644f
更新日期：1997-08-05 00:00:00
abstract：:The manganese transport regulator (MntR) of Bacillus subtilis is activated by Mn(2+) to repress transcription of genes encoding transporters involved in the uptake of manganese. MntR is also strongly activated by cadmium, both in vivo and in vitro, but it is poorly activated by other metal cations, including calcium a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0524215
更新日期：2006-03-21 00:00:00
abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00439a004
更新日期：1989-06-27 00:00:00
abstract：:Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00346a006
更新日期：1985-12-03 00:00:00
abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0355239
更新日期：2003-12-16 00:00:00
abstract：:The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the transition state for GPDH-catalyzed ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00116
更新日期：2016-03-15 00:00:00
abstract：:We describe the crystal structure of human epidermal-type fatty acid binding protein (E-FABP) that was recently found to be highly upregulated in human psoriatic keratinocytes. To characterize E-FABP with respect to ligand-binding properties and tertiary structure, we cloned the respective cDNA, overexpressed the prot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990305u
更新日期：1999-09-21 00:00:00
abstract：:The Wiskott-Aldrich syndrome protein (WASP) and neural WASP (N-WASP) are key players in regulating actin cytoskeleton via the Arp2/3 complex. It has been widely reported that the WASP proteins are activated by Rho family small GTPase Cdc42 and that Rac1 acts through SCAR/WAVE proteins. However, a systematic study of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062152y
更新日期：2007-03-20 00:00:00
abstract：:Inducible dimers are powerful tools for controlling biological processes through colocalizing signaling molecules. To be effective, an inducible system should have a dissociation constant in the "off" state that is greater (i.e., weaker affinity) than the concentrations of the molecules that are being controlled, and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00529
更新日期：2016-09-20 00:00:00
abstract：:Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00042a028
更新日期：1995-10-24 00:00:00
abstract：:Nonribosomal peptide synthetases (NRPSs) are megasynthetases that require complex and specific interactions between multiple domains and proteins to functionally produce a metabolite. MbtH-like proteins (MLPs) are integral components of many NRPSs and interact directly with the adenylation domain of the megasynthetase...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00552
更新日期：2018-07-17 00:00:00
abstract：:NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a highly complicated, energy transducing, membrane-bound enzyme. It contains 46 different subunits and nine redox cofactors: a noncovalently bound flavin mononucleotide and eight iron-sulfur clusters. The mechanism of complex I is not known. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051809x
更新日期：2006-01-10 00:00:00
abstract：:There are a small number of peptides derived from proteins that have a propensity to adopt structure in aqueous solution which is similar to the structure they possess in the parent protein. There are far fewer examples of protein fragments which adopt stable nonnative structures in isolation. Understanding how nonnat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990320z
更新日期：1999-06-01 00:00:00
