Elsamicin A can convert the Z-form of poly[d(G-C)] and poly[(G-m5C)] back to B-form DNA.

abstract：:A full-length cDNA encoding a calcium-dependent protein kinase with a calmodulin-like domain from Arabidopsis thaliana (AK-1 for Arabidopsis kinase-1) has been expressed as a fusion protein (called AK-1-6H) in Escherichia coli and purified to near homogeneity with high specific activity (typically 2000 nmol min-1 mg-1...

journal_title：Biochemistry

authors： Binder BM,Harper JF,Sussman MR

更新日期：1994-03-01 00:00:00

abstract：:Oligomers of amyloid-β (Aβ) have emerged as the primary toxic agents responsible for early synaptic dysfunction and neuronal death in Alzheimer's disease (AD). Characterization of oligomers is an important step in the progress toward delineating the complex molecular mechanisms involved in AD pathogenesis. In our prev...

journal_title：Biochemistry

authors： Dean DN,Pate KM,Moss MA,Rangachari V

更新日期：2016-04-19 00:00:00

abstract：:The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...

journal_title：Biochemistry

authors： Doukov T,Li H,Soltis M,Poulos TL

更新日期：2009-11-03 00:00:00

abstract：:The distances between the anticodon loops of fluorescent tRNAPhe bound to the E site and to either the A or the P site of poly(U)-programmed Escherichia coli ribosomes were measured by fluorescence energy transfer. Donor and acceptor molecules were wybutine and proflavin, respectively, both located 3' to the anticodon...

journal_title：Biochemistry

authors： Paulsen H,Wintermeyer W

更新日期：1986-05-20 00:00:00

abstract：:Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...

journal_title：Biochemistry

authors： Arnold WR,Baylon JL,Tajkhorshid E,Das A

更新日期：2017-12-26 00:00:00

abstract：:The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...

journal_title：Biochemistry

authors： Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

更新日期：1992-12-08 00:00:00

abstract：:TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal lobar degeneration (FTLD). Wild type TDP-43 inclusions are a pathological hallmark of >95% ...

journal_title：Biochemistry

authors： Ng JSW,Hanspal MA,Matharu NS,Barros TP,Esbjörner EK,Wilson MR,Yerbury JJ,Dobson CM,Kumita JR

更新日期：2019-10-01 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:The purL gene of Escherichia coli encoding the enzyme formylglycinamidine ribonucleotide (FGAM) synthetase which catalyzes the conversion of formylglycinamide ribonucleotide (FGAR), glutamine, and MgATP to FGAM, glutamate, ADP, and Pi has been cloned and sequenced. The mature protein, as deduced by the structural gene...

journal_title：Biochemistry

authors： Schendel FJ,Mueller E,Stubbe J,Shiau A,Smith JM

更新日期：1989-03-21 00:00:00

abstract：:Dopamine (DA) is an unstable neurotransmitter that readily oxidizes to the DA quinone and forms reactive oxygen species, such as superoxide and hydrogen peroxide. The oxidized dopamine also forms thiol conjugates with sulfhydryl groups on cysteine, glutathione, and proteins. In the present study, we determined the red...

journal_title：Biochemistry

authors： Akagawa M,Ishii Y,Ishii T,Shibata T,Yotsu-Yamashita M,Suyama K,Uchida K

更新日期：2006-12-19 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:The majority of pre-steady-state kinetic investigations with HIV-1 reverse transcriptase (HIV-1 RT) have reported substoichiometric bursts (30-50%) of product formation in the initial reaction cycle. By using quantitative amino acid analysis, we have revised the extinction coefficient of the HIV-1 RT heterodimer and s...

journal_title：Biochemistry

authors： Furge LL,Guengerich FP

更新日期：1999-04-13 00:00:00

abstract：:Elongation factor Tu (EF-Tu) is a highly conserved GTPase that is responsible for supplying the aminoacylated tRNA to the ribosome. Upon binding to the ribosome, EF-Tu undergoes GTP hydrolysis, which drives a major conformational change, triggering the release of aminoacylated tRNA to the ribosome. Using a combination...

journal_title：Biochemistry

authors： Lai J,Ghaemi Z,Luthey-Schulten Z

更新日期：2017-11-14 00:00:00

abstract：:Stopped-flow measurements have been employed to study the kinetics of the conformational changes in TetR (B) induced by tetracycline binding with and without Mg(2+) ions. Result of stopped-flow fluorometry measurements at pH 8.0 indicate conformational changes in the helix-turn-helix motif in the N-terminal domain and...

journal_title：Biochemistry

authors： Kedracka-Krok S,Gorecki A,Bonarek P,Wasylewski Z

更新日期：2005-01-25 00:00:00

abstract：:Coumermycin A(1) is a member of the aminocoumarin family of antibiotics. Unlike its structural relatives, novobiocin and clorobiocin, coumermycin A(1) is a dimer built on a 3-methyl-2,4-dicarboxypyrrole scaffold and bears two decorated noviose sugar components which are the putative target binding motifs for DNA gyras...

journal_title：Biochemistry

authors： Freel Meyers CL,Oberthür M,Heide L,Kahne D,Walsh CT

更新日期：2004-11-30 00:00:00

abstract：:The photocycle of the blue-light photoreceptor protein Photoactive Yellow Protein (PYP) was studied at reduced relative humidity (RH). Photocycle kinetics and spectra were measured in thin films of PYP in which the relative humidity was set at values between 29 and 98% RH with saturated solutions of various salts. We ...

journal_title：Biochemistry

authors： van der Horst MA,van Stokkum IH,Dencher NA,Hellingwerf KJ

更新日期：2005-06-28 00:00:00

abstract：:The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent for M...

journal_title：Biochemistry

authors： Tekos A,Stathopoulos C,Drainas D

更新日期：1998-11-03 00:00:00

abstract：:In order to more clearly define the structure of human plasma fibronectin (PFn) under physiologic buffer conditions, we determined the mean harmonic rotational relaxation times (rho H) of PFn and the thrombin-derived 190/170-kDa PFn fragment using steady-state fluorescence polarization. These measurements utilized the...

journal_title：Biochemistry

authors： Benecky MJ,Kolvenbach CG,Wine RW,DiOrio JP,Mosesson MW

更新日期：1990-03-27 00:00:00

abstract：:The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns...

journal_title：Biochemistry

authors： Westrick JA,Peters KS,Ropp JD,Sligar SG

更新日期：1990-07-17 00:00:00

abstract：:We have previously shown that introduction of an engineered Met160 residue in ascorbate peroxidase (S160M variant) leads to the formation of a covalent link between Met160 and the heme vinyl group [Metcalfe, C. L., et al. (2004) J. Am. Chem. Soc. 126, 16242-16248]. In this work, we have used electronic spectroscopy, H...

journal_title：Biochemistry

authors： Pipirou Z,Bottrill AR,Svistunenko DA,Efimov I,Basran J,Mistry SC,Cooper CE,Raven EL

更新日期：2007-11-20 00:00:00

abstract：:The organization of the higher order structure of chromatin has been examined in chicken erythrocyte. Chromatin solubilized during the time course of a gentle micrococcal nuclease digestion of nuclei shows a continuous variation in the distribution of molecular weights. Electron microscopy studies of large chromatin f...

journal_title：Biochemistry

authors： Ruiz-Carrillo A,Puigdomènech P,Eder G,Lurz R

更新日期：1980-06-10 00:00:00

abstract：:The carbohydrate binding specificity of the seed lectin from Artocarpus integrifolia, artocarpin, has been elucidated by the enzyme-linked lectin absorbent assay [Misquith, S., et al (1994) J. Biol. Chem. 269, 30393-30401], wherein it was demonstrated to be a Man/Glc specific lectin with high affinity for the trisacch...

journal_title：Biochemistry

authors： Rani PG,Bachhawat K,Reddy GB,Oscarson S,Surolia A

更新日期：2000-09-05 00:00:00

abstract：:The cardiac-specific Nkx2.5 homeodomain has been expressed as a 79-residue protein with the oxidizable Cys(56) replaced with Ser. The Nkx2.5 or Nkx2.5(C56S) homeodomain is 73% identical in sequence to and has the same NMR structure as the vnd (ventral nervous system defective)/NK-2 homeodomain of Drosophila when bound...

journal_title：Biochemistry

authors： Fodor E,Mack JW,Maeng JS,Ju JH,Lee HS,Gruschus JM,Ferretti JA,Ginsburg A

更新日期：2005-09-20 00:00:00

abstract：:The electron paramagnetic resonance (EPR) spectra of spin-labeled DNA duplexes, both bound to DEAE-Sephadex and free in solution, have been analyzed. The nitroxide spin-labels are covalently linked to a deoxyuridine residue using either a monoacetylene or diacetylene tether. This difference in tether length produces a...

journal_title：Biochemistry

authors： Hustedt EJ,Kirchner JJ,Spaltenstein A,Hopkins PB,Robinson BH

更新日期：1995-04-04 00:00:00

abstract：:In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...

journal_title：Biochemistry

authors： Munson LM,Reznikoff WS

更新日期：1981-04-14 00:00:00

abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

journal_title：Biochemistry

authors： Beard WA,Wilson SH

更新日期：2014-05-06 00:00:00

abstract：:The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...

journal_title：Biochemistry

authors： Benson TE,Marquardt JL,Marquardt AC,Etzkorn FA,Walsh CT

更新日期：1993-03-02 00:00:00

abstract：:To probe the details of small amplitude motions in proteins, a dynamical analysis of the orientation fluctuations of two tyrosine side chains in the bovine pancreatic trypsin inhibitor is presented. Detailed results are given for the time history and correlation functions obtained for the ring motion from a molecular ...

journal_title：Biochemistry

authors： McCammon JA,Wolynes PG,Karplus M

更新日期：1979-03-20 00:00:00

abstract：:NanGIR1 is a catalytic element inserted in the P6 loop of a group I intron (NanGIR2) in the small subunit rRNA precursor of the protist Naegleria andersoni [Einvik, C., Decatur, W. A., Embley, T. M., Vogt, V. M., and Johansen, S. (1997) RNA 3, 710-720]. It catalyzes site-specific hydrolysis at an internal processing s...

journal_title：Biochemistry

authors： Jabri E,Aigner S,Cech TR

更新日期：1997-12-23 00:00:00

abstract：:The denaturation of the heat shock protein DnaK induced by guanidine hydrochloride (Gdn-HCl) was investigated by circular dichroism, fluorescence, size-exclusion HPLC, and dynamic light scattering. DnaK unfolding takes place in two discrete steps. The midpoint (Cm) of the first transition (0.5 M) was shifted to higher...

journal_title：Biochemistry

authors： Palleros DR,Shi L,Reid KL,Fink AL

更新日期：1993-04-27 00:00:00