Abstract:
:Dopamine (DA) is an unstable neurotransmitter that readily oxidizes to the DA quinone and forms reactive oxygen species, such as superoxide and hydrogen peroxide. The oxidized dopamine also forms thiol conjugates with sulfhydryl groups on cysteine, glutathione, and proteins. In the present study, we determined the redox potential of the protein-bound DA and established a novel mechanism for the oxidative modification of the protein, in which the DA-cysteine adduct generated in the DA-modified protein causes oxidative modification of the DA-bound protein in the presence of Cu2+. Exposure of a sulfhydryl enzyme, glyceraldehyde-3-phosphate dehydrogenase, to DA resulted in a significant loss of sulfhydryl groups and the formation of the DA-cysteine adduct. When the DA-modified protein was incubated with Cu2+, we observed aggregation and degradation of the DA-bound protein and concomitant formation of a protein carbonyl, a marker of an oxidatively modified protein. Furthermore, we analyzed the carbonyl products generated during the Cu2+-catalyzed oxidation of the DA-modified protein and revealed the production of glutamic and aminoadipic semialdehydes, consisting of the protein carbonyls generated. The cysteinyl-DA residue generated in the DA-modified protein was suggested to represent a redox-active adduct, based on the observations that the cysteinyl-DA adduct, 5-S-cysteinyldopamine, produced by the reaction of cysteine with DA, gave rise to the oxidative modification of bovine serum albumin in the presence of Cu2+. These data suggest that the DA-modified protein may be involved in redox alteration under oxidative stress, whereby DA covalently binds to cysteine residues, generating the redox-active cysteinyl-DA adduct that causes the metal-catalyzed oxidation of protein.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Akagawa M,Ishii Y,Ishii T,Shibata T,Yotsu-Yamashita M,Suyama K,Uchida Kdoi
10.1021/bi0614434subject
Has Abstractpub_date
2006-12-19 00:00:00pages
15120-8issue
50eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The recently isolated Escherichia coli murB gene (Pucci et al., 1992) has been cloned into an expression vector and the encoded UDP-N-acetylenolpyruvylglucosamine reductase (EC 1.1.1.158) was overproduced to about 10% of soluble cell protein. The encoded 38-kDa protein has been purified to near homogeneity. It was fou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a019
更新日期:1993-03-02 00:00:00
abstract::The alpha-helix [Pauling, L., Corey, R. B., & Branson, H. R. (1951) Proc. Natl. Acad. Sci. U.S.A. 37, 205-211] is a common motif in both proteins and peptides. Despite intense investigation, predictive understanding of helices is still lacking. A recent hypothesis [Presta, L. G., & Rose, G. D. (1988) Science 240, 1632...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00081a001
更新日期:1993-08-03 00:00:00
abstract::Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a004
更新日期:1989-06-27 00:00:00
abstract::We have examined the competitive binding of oxygen and carbon monoxide to the multisubunit hemocyanin of the tarantula Eurypelma californicum. Employment of high-precision thin-layer methods has enabled detailed characterization of the pure oxygen and pure carbon monoxide binding curves, as well as binding curves perf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00418a036
更新日期:1988-09-06 00:00:00
abstract::Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701616r
更新日期:2008-02-05 00:00:00
abstract::1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00160a029
更新日期:1992-11-17 00:00:00
abstract::The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00177a016
更新日期:1994-03-22 00:00:00
abstract::A peptide corresponding to a portion of the amphipathic alpha-helical region of CTP:phosphocholine cytidylyltransferase was synthesized. This region of the enzyme was proposed to be the membrane-binding domain [Kalmar, G.B., Kay, R.J., Lachance, A., Aebersold, R., & Cornell, R.B. (1990) Proc. Natl. Acad. Sci. U.S.A. 8...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00180a029
更新日期:1994-04-12 00:00:00
abstract::Proline utilization A (PutA) from Escherichia coli is a multifunctional flavoprotein that is both a transcriptional repressor of the proline utilization (put) genes and a membrane-associated enzyme which catalyzes the 4-electron oxidation of proline to glutamate. Previously, proline was shown to induce PutA-membrane b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048596g
更新日期:2004-10-19 00:00:00
abstract::3-Fluorooxalacetate is a substrate for malic dehydrogenase. When enzymatic reduction is slower than the rate of epimerization of the two enantiomers, only (2R,3R)-erythro-fluoromalate is formed. Conversely, when a high enzyme level and excess of NADH lead to reduction that is fast relative to the epimerization rate, e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981819g
更新日期:1998-12-22 00:00:00
abstract::ZFY, a male-associated Zn-finger protein encoded by the human Y chromosome, exhibits a distinctive two-finger repeat: whereas odd-numbered domains fit a general consensus, even-numbered domains exhibit systematic differences. Do these odd and even sequences encode structurally distinct surfaces for DNA recognition? As...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00243a005
更新日期:1991-07-23 00:00:00
abstract::The membrane-bound beta-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae catalyzes the decarboxylation of carboxybiotin, which is coupled to Na(+) translocation and consumes a periplasmically derived proton. Upon site-directed mutagenesis of 20 polar and/or conserved residues within putative membrane-i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992817e
更新日期:2000-04-18 00:00:00
abstract::Allosteric activators are generally believed to shift the equilibrium distribution of enzyme conformations to favor a catalytically productive structure; the kinetics of conformational exchange is seldom addressed. Several observations suggested that the usual allosteric mechanism might not apply to the activation of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200785s
更新日期:2011-10-04 00:00:00
abstract::DNA-dependent synthesis of beta-galactosidase was optimized in extracts made from cells lysed by a standard French pressure cell. Extracts made at 3200 psi synthesized up to 25-fold more beta-galactosidase than extracts made at 7500 psi. beta-Galactosidase synthesis was cyclic 3', 5' AMP dependent, as expected, and in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00624a016
更新日期:1977-03-08 00:00:00
abstract::Solution X-ray scattering patterns of enzyme I of the phosphotransferase system from Staphylococcus carnosus indicate an increase in radius of gyration and molecular mass in the presence of Mg2+ or both Mg2+ and phosphoenolpyruvate, indicating a partial dimerization of enzyme I. Mg2+ ions are essential for both the di...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00048a014
更新日期:1995-12-05 00:00:00
abstract::Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00201a018
更新日期:1994-09-06 00:00:00
abstract::Atlantic salmon fast skeletal muscle is composed of an α-type tropomyosin that shares 20 substitutions with a mammalian homologue. Prominent isomorphisms considered to be potentially destabilizing include threonine 179 (core, Ala in rabbit) and a unique pair of glycines, residues 24 and 27. Bacterially expressed mutan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00156
更新日期:2015-05-05 00:00:00
abstract::Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025654u
更新日期:2002-06-25 00:00:00
abstract::The three-dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization. The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from bioche...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00019a030
更新日期:1995-05-16 00:00:00
abstract::The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00046a043
更新日期:1995-11-21 00:00:00
abstract::The mechanism of binding of the substrates Mg x ATP and ribose 5-phosphate as well as Mg2+ to the enzyme 5-phospho-D-ribosyl (alpha-1-diphosphate synthetase from Escherichia coli has been analyzed. By use of the competive inhibitors of ATP and ribose 5-phosphate binding, alpha,beta-methylene ATP and (+)-1-alpha,2-alph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962610a
更新日期:1997-04-22 00:00:00
abstract::We have validated that ligand peptides designed from antigen peptides could be used for targeting specific major histocompatibility complex class I (MHC-I) molecules on the cell surface. To design the ligand peptides, we used reported antigen peptides for each MHC-I molecule with high binding affinity. From the crysta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00735
更新日期:2020-12-15 00:00:00
abstract::Gelatinase B is a matrix metalloproteinase (MMP-9) involved in tissue remodeling, development, cancer, and inflammation. Neutrophils produce three major forms of (pro)gelatinase B: 92 kDa monomers, homodimers, and complexes of gelatinase B covalently bound to neutrophil gelatinase B-associated lipocalin (NGAL). In con...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991162e
更新日期:1999-10-19 00:00:00
abstract::When different mutations were introduced into the anticodon loop and at position 73 of YFA2, a derivative of yeast tRNA(Phe), a single tRNA body was misacylated with 13 different amino acids. The affinities of these misacylated tRNAs for Thermus thermophilus elongation factor Tu (EF-Tu).GTP were determined using a rib...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036290o
更新日期:2004-05-25 00:00:00
abstract::Membrane vesicles can be prepared from murine lymphoid cells by nitrogen cavitation and fractionated by sedimentation through nonlinear sucrose density gradients. Two subpopulations of membrane vesicles, PMI and PMII, can be distinguished on the basis of sedimentation rate. The subcellular distribution of adenylate an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00652a026
更新日期:1976-04-06 00:00:00
abstract::In search of the regulation mechanisms for isoform specific myosin assembly, we have used the COOH-terminal fragments of nonmuscle myosin isoforms MIIA and MIIB (MIIA(F46) and MIIB(alpha)(F47)) as a model system. Phosphorylation by protein kinase C (PK C) or casein kinase II (CK II) within or near the nonhelical tail-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000347e
更新日期:2000-09-19 00:00:00
abstract::The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00613a031
更新日期:1978-10-03 00:00:00
abstract::In order to test the proposal [Stehle, T., Claiborne, A., & Schulz, G. E. (1993) Eur. J. Biochem. 211, 221-226] that the active-site His10 of NADH peroxidase functions as an essential acid-base catalyst, we have analyzed mutants in which this residue has been replaced by Gln or Ala. The k(cat) values for both H10Q and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952347y
更新日期:1996-02-20 00:00:00
abstract::The role of acidic amino acid residues in cation recognition and selectivity by the Na+/dicarboxylate cotransporter, NaDC-1, was investigated by site-directed mutagenesis and expression in Xenopus oocytes. Four of the residues tested, Asp-52, Glu-74, Glu-101, and Glu-332, were found to be unimportant for transport act...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990076b
更新日期:1999-06-08 00:00:00
abstract::We studied the binding characteristics of a synthetic 20-residue peptide to supported single planar bilayers of phosphatidylcholine, and the orientation of the peptide by Fourier-transform infrared spectroscopy with an attenuated total reflection method. This peptide, designed to resemble a putative fusion peptide of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952547+
更新日期:1996-04-16 00:00:00