Abstract:
:The membrane-bound beta-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae catalyzes the decarboxylation of carboxybiotin, which is coupled to Na(+) translocation and consumes a periplasmically derived proton. Upon site-directed mutagenesis of 20 polar and/or conserved residues within putative membrane-integral regions, the specific oxaloacetate decarboxylase activities were reduced to various extents, but only the enzyme with a Y229F mutation was completely inactive. We propose that Y229 is part of the network by which the proton of S382 is delivered to carboxybiotin, where it is consumed upon catalyzing the immediate decarboxylation of this acid-labile compound. Unlike S382 or D203, Y229 appears to be not involved in Na(+) binding, because in the Y229F orY229A mutants, the beta-subunit was protected from tryptic digestion by 50 mM NaCl like in the wild-type enzyme. Oxaloacetate decarboxylase with a betaC291E mutation was unstable in the absence of Na(+) and dissociated into an alpha-gamma subcomplex and the beta-subunit. The enzyme could only be isolated in the presence of 0. 5 M NaCl. These results are consistent with the notion that the beta-subunit changes its conformation upon Na(+) binding.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Jockel P,Schmid M,Choinowski T,Dimroth Pdoi
10.1021/bi992817esubject
Has Abstractpub_date
2000-04-18 00:00:00pages
4320-6issue
15eissn
0006-2960issn
1520-4995pii
bi992817ejournal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The properties of the nucleotide binding sites in the isolated beta and alpha subunits of H(+)-ATPase from Bacillus PS3 (TF1) have been examined by studying the EPR properties of bound VO(2+), which is a paramagnetic probe for the native Mg2+ cation cofactor. The amino acid ligands of the VO2+ complexes with the isola...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9910732
更新日期:1999-11-16 00:00:00
abstract::Many DNA polymerases select their natural substrates, deoxy- as opposed to ribonucleoside triphosphates, with a selectivity greater than 10000-fold. The function of a highly conserved residue, Tyr416, in the palm domain of the parental enzyme, an exo(-) derivative of RB69 DNA polymerase (gp43), a member of the pol alp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0202171
更新日期:2002-08-13 00:00:00
abstract::DNA sequences containing homopurine d(G1-3A)n tracts are known to be capable of adopting non-B-DNA conformations. The structural polymorphism of these sequences is a direct consequence of the structural properties of the homopurine d(G1-3A)n tracts. Depending on the conditions, d(GA)n DNA sequences can form antiparall...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961020+
更新日期:1996-10-08 00:00:00
abstract::Beetle luciferases produce different bioluminescence colors from green to red using the same d-luciferin substrate. Despite many studies of the mechanisms and structural determinants of bioluminescence colors with firefly luciferases, the identity of the emitters and the specific active site interactions responsible f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500160m
更新日期:2014-08-19 00:00:00
abstract::Nicotinic acetylcholine receptors (nAChRs) were studied in detail in the past regarding their interaction with therapeutic and drug addiction related compounds. Using fast kinetic whole-cell recording, we have now studied effects of tacrine, an agent used clinically to treat Alzheimer's disease, on currents elicited b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101789y
更新日期:2011-03-22 00:00:00
abstract::Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011929e
更新日期:2002-04-02 00:00:00
abstract::Two series of experiments were carried out to characterize (a) peptide fragments of sarcoplasmic reticulum (SR) ATPase, based on proteolysis with different enzymes and distribution of known labels, and (b) specific labeling and functional inactivation patterns, following ATPase derivatization with dicyclohexylcarbodii...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00405a061
更新日期:1988-03-08 00:00:00
abstract::We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972455f
更新日期:1998-03-17 00:00:00
abstract::We consider the effects that different lipid surfaces have upon the denaturation and subsequent formation of amyloid fibrils of bovine insulin. The adsorption and unfolding kinetics of insulin being adsorbed onto the different lipid surfaces under denaturing conditions are studied using FTIR ATR spectroscopy and are c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020525z
更新日期:2002-12-31 00:00:00
abstract::The channel of the purified acetylcholine receptor from Torpedo californica electric organ reconstituted in lipid vesicles was assayed by direct electrical recording using patch-clamp pipets. High-resistance seals were obtained by gentle suction of vesicles into the pipet or after the formation of lipid bilayers from ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a003
更新日期:1983-05-10 00:00:00
abstract::The spontaneous hydrolyses of lactic acid O-phenyl phosphate (I) and, to a lesser extent, 3-hydroxybutyric acid O-phenyl phosphate (II) have been investigated and compared with similar intramolecular and bimolecular reactions. Compared to bimolecular nucleophilic reactions, the reactivity of II is similar to other sys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00682a025
更新日期:1975-06-03 00:00:00
abstract::The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00583a005
更新日期:1979-08-07 00:00:00
abstract::The field of chemical site-selective modification of proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use of site-selective protein chemistry to probe the ro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00639
更新日期:2018-10-30 00:00:00
abstract::Immunological methods, in parellel with measurement of ATPase activity, have been used to characterize the reactions of antibodies specific for light chains with myosin and its water-soluble proteolytic subfragments, heavy meromyosin (HMM) and subfragment 1 (HMM S-1). Antiserum to the 5,5'-dithiobis(2-nitro-enzoic aci...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00692a008
更新日期:1975-10-21 00:00:00
abstract::Ca2+ release from heavy sarcoplasmic reticulum (SR) vesicles was induced by 2 mM caffeine, and the amount (A) and the rate constant (k) of Ca2+ release were investigated as a function of the extent of Ca2+ loading. Under both passive and active loading conditions, the A value increased monotonically in parallel to Ca2...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00442a033
更新日期:1989-08-08 00:00:00
abstract::Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050595g
更新日期:2005-09-20 00:00:00
abstract::MenB, the 1,4-dihydroxy-2-naphthoyl-CoA synthase from the bacterial menaquinone biosynthesis pathway, catalyzes an intramolecular Claisen condensation (Dieckmann reaction) in which the electrophile is an unactivated carboxylic acid. Mechanistic studies on this crotonase family member have been hindered by partial acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200877x
更新日期:2011-11-08 00:00:00
abstract::It is well-known that caldesmon (CaD) is a substrate for casein kinase II (CKII), and the phosphorylation of CaD by CKII regulates the interaction of CaD with myosin. However, the functionally relevant CKII phosphorylation site(s) on CaD and the precise role of CaD phosphorylation by CKII in mediating CaD's function h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0006767
更新日期:2000-09-12 00:00:00
abstract::The thermal denaturation of the cupredoxin plastocyanin (PC) from spinach has been studied with the aim of improving the understanding of factors involved in the conformational stability of antiparallel beta-sheet proteins. Studies using differential scanning calorimetry have been complemented with nuclear magnetic re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034371e
更新日期:2003-09-02 00:00:00
abstract::The activation of adenosine-5'-monophosphate aminohydrolase from rabbit skeletal muscle by H+ has been demonstrated. Evidence is presented which indicates that the binding of H+ and K+ is linked, in that the dissociation constant (KA) for K+ activation is reduced as the pH is lowered. Concomitantly, the pK of several ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00641a013
更新日期:1977-11-01 00:00:00
abstract::The allosteric activation of the T127-->L mutant of 3',5'-cyclic adenosine monophosphate (cAMP) receptor protein (CRP) by cAMP changes from an exothermic, independent two-site binding mechanism at pH 7.0 to an endothermic, interacting two-site binding mechanism at pH 5.2, similar to that observed for CRP at pH 7.0 and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000225m
更新日期:2000-06-20 00:00:00
abstract::Chicken nucleoside triphosphate diphosphohydrolase 8 (NTPDase8) is a cell surface ectonucleotidase with a large extracellular domain (ECD) containing the active site and is anchored to the membrane by two transmembrane domains (TMDs) at the N- and C-termini. Unlike other cell surface NTPDases that have been characteri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901820c
更新日期:2010-01-12 00:00:00
abstract::One of the key pathological features of Alzheimer's disease is the aggregation of tau protein. We are therefore searching for compounds capable of inhibiting this reaction. On the basis of an initial screen of 200000 compounds [Pickhardt, M., Gazova, Z., von Bergen, M., Khlistunova, I., Wang, Y., Hascher, A., Mandelko...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700878g
更新日期:2007-09-04 00:00:00
abstract::To identify the specific component(s) in the target membrane involved in fusion of vesicular stomatitis virus (VSV), we examined the interaction of the virus with human erythrocyte membranes with asymmetric and symmetric bilayer distributions of phospholipids. Fusion was monitored spectrofluorometrically by the octade...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00469a005
更新日期:1990-05-01 00:00:00
abstract::The hairpin catalytic motif in tobacco ringspot virus satellite RNA consists of two helix-loop-helix elements on two adjacent arms of a four-way helical junction. The bases essential for catalytic activity are located in the loops that are brought into proximity by a conformational change as a prerequisite for catalys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi010773f
更新日期:2001-09-18 00:00:00
abstract::The mechanisms that control N protein dependent antitermination in phage lambda have counterparts in many eukaryotic systems, including specific regulatory interactions of the antitermination protein with the nascent RNA transcript. Here we describe the specific and nonspecific RNA binding modes of antitermination pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961920q
更新日期:1997-02-11 00:00:00
abstract::To better understand the factors controlling the binding of apolipoprotein molecules at the surfaces of serum lipoprotein particles, the adsorption of human apolipoprotein A-I to phospholipid monolayers has been studied. The influence of lipid packing was investigated by spreading the monolayers at various initial sur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00418a073
更新日期:1988-09-06 00:00:00
abstract::The occurrence of isozymes in plant peroxidases is poorly understood. Turnip roots contain seven season-dependent isoperoxidases with distinct physicochemical properties. In the work presented here, multifrequency electron paramagnetic resonance spectroscopy has been used to characterize the Compound I intermediate ob...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002826j
更新日期:2001-06-12 00:00:00
abstract::NADP(H) binding is essential for fast electron transfer through the flavoprotein domain of the fusion protein P450BM3. Here we characterize the interaction of NADP(H) with the oxidized and partially reduced enzyme and the effect of this interaction on the redox properties of flavin cofactors and electron transfer. Mea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001068u
更新日期:2000-10-17 00:00:00
abstract::Peptidylglycine alpha-hydroxylating monooxygenase (PHM) is a copper, ascorbate, and molecular oxygen dependent enzyme that catalyzes the first step leading to the C-terminal amidation of glycine-extended peptides. The catalytic core of PHM (PHMcc), refined to residues 42-356 of the PHM protein, was expressed at high l...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9708747
更新日期:1997-09-09 00:00:00