Abstract:
:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard tissue) for bone or dental tissue engineering. Realizing the natural affinity of ECM components toward inorganic constituents of hard tissue, we successfully constructed the nanohybrids of DNA/CaP particles with either collagen 1 or fibronectin, which finally possessed the capability of specific recognition of integrin receptor for being swiftly internalized across the plasma membrane, leading to remarkably high transgene expression in mammalian cells. This new approach with precise receptor-specific delivery as well as 10- to 50-fold enhanced efficiency level compared to the classical one, has immediate applications for basic research and large scale production of recombinant therapeutic proteins and looks promising for gene therapy.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike Tdoi
10.1021/bi050595gsubject
Has Abstractpub_date
2005-09-20 00:00:00pages
12273-8issue
37eissn
0006-2960issn
1520-4995journal_volume
44pub_type
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