Abstract：
:The synthetic peptide SNX-111 corresponding to the sequence of the omega-conopeptide MVIIA from the venom of the marine snail Conus magus is a highly potent and selective antagonist of N-type calcium channels. We have synthesized and characterized a large number of analogs of SNX-111 in order to elucidate the structural features of the peptide involved in blocking N-type calcium channels. Comparison of the binding of SNX-111 and its analogs to rat brain synaptosomal membranes rich in N-type channels revealed that, among the four lysines and two arginines in the molecule, lysine in position 2 and arginines at position 10 and 21 are important for the interaction of SNX-111 with N-type channels. The importance of the middle segment from residues 9 through 14 for this binding interaction was revealed by substitution of the individual residues as well as by the construction of hybrid peptides in which the residues 9-12 in SNX-111 and another conopeptide, SNX-183, corresponding to a peptide SVIB from Conus striatus, were interchanged. Introduction of the sequence SRLM from SNX-111 in place of RKTS in position 9-12 in SNX-183 resulted in a 38-fold increase in affinity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Nadasdi L,Yamashiro D,Chung D,Tarczy-Hornoch K,Adriaenssens P,Ramachandran Jdoi
10.1021/bi00025a013subject
Has Abstractpub_date
1995-06-27 00:00:00pages
8076-81issue
25eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201880s
更新日期：2012-02-21 00:00:00
abstract：:Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical properties--elastic moduli and level of tension at membrane rupture--w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00413a013
更新日期：1988-06-28 00:00:00
abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a033
更新日期：1977-11-29 00:00:00
abstract：:The hydrophobic, membrane-binding domain of purified human erythrocyte acetylcholinesterase was labeled with the photoactivated reagent 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine. The radiolabel was incorporated when the enzyme was prepared in detergent-free aggregates, in detergent micelles, or in phospholip...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00359a004
更新日期：1986-06-03 00:00:00
abstract：:Binding of the coupling ion (Na+ or Li+) and sugars to the purified melibiose permease of Escherichia coli, reconstituted in proteoliposomes, produces selective and cooperative changes of the transporter tryptophan fluorescence. To assess the individual contribution of N- or C-terminal domains of the permease to these...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961372g
更新日期：1996-09-17 00:00:00
abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00430a033
更新日期：1989-02-21 00:00:00
abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991199z
更新日期：2000-02-15 00:00:00
abstract：:Hepatic lipase (HL) hydrolysis of phosphatidylcholine (PC) was studied in recombinant high-density lipoprotein particles (r-HDL). r-HDL were made from cholate mixed micelles that contained PC, apo AI, and, in some cases, unesterified cholesterol. r-HDL were characterized using chemical composition, nondenaturing gradi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970356w
更新日期：1997-10-07 00:00:00
abstract：:The kinetics of self-association for beef liver glutamate dehydrogenase (EC 1.4.1.3) have been measured by using pressure perturbation in both the time domain and the frequency domain by monitoring scattered light intensity. The kinetic behavior is entirely consistent with the random self-association model proposed by...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00579a007
更新日期：1979-06-12 00:00:00
abstract：:Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962203z
更新日期：1996-12-17 00:00:00
abstract：:Choline oxidase catalyzes the oxidation of choline to glycine betaine through a two-step, four-electron reaction with betaine aldehyde as an intermediate. Oxygen is the final electron acceptor. Alcohol oxidation is initiated by the removal of the substrate hydroxyl proton by an unknown active site residue with a pKa v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500982y
更新日期：2015-01-20 00:00:00
abstract：:Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9002265
更新日期：2009-06-09 00:00:00
abstract：:Several diseases, such as cancer, are characterized by acidification of the extracellular environment. Acidosis can be employed as a target to specifically direct therapies to the diseased tissue. We have used first principles to design an acidity-triggered rational membrane (ATRAM) peptide with high solubility in sol...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00856
更新日期：2015-11-03 00:00:00
abstract：:Human DNA is continuously damaged by exogenous and endogenous genotoxic insults. To counteract DNA damage and ensure the completion of DNA replication, cells possess specialized DNA polymerases (Pols) that bypass a variety of DNA lesions. Human DNA polymerase kappa (hPolkappa) is a member of the Y-family of DNA Pols a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900153t
更新日期：2009-05-26 00:00:00
abstract：:The solution conformation of potato carboxypeptidase inhibitor (CPI) has been investigated by 1H NMR spectroscopy. The spectrum is assigned in a sequential manner by using two-dimensional NMR techniques to identify through-bond and through-space (less than 5 A) connectivities. A set of 309 approximate interproton dist...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00398a069
更新日期：1987-12-01 00:00:00
abstract：:We have characterized the kinetics and substrate requirements of prenyl-flavin synthase from yeast. This enzyme catalyzes the addition of an isopentenyl unit to reduced flavin mononucleotide (FMN) to form an additional six-membered ring that bridges N5 and C6 of the flavin nucleus, thereby converting the flavin from a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01131
更新日期：2018-02-06 00:00:00
abstract：:The DNA repair enzyme uracil DNA glycosylase has been crystallized with a cationic 1-aza-2'-deoxyribose-containing DNA that mimics the ultimate transition state of the reaction in which the water nucleophile attacks the anomeric center of the oxacarbenium ion-uracil anion reaction intermediate. Comparison with substra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035372+
更新日期：2003-11-04 00:00:00
abstract：:RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Many of these RNA binding proteins occur as gene families with members sharing a high degree of primary structure identity and domain organization yet have tissue-specific expression patterns a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00256
更新日期：2018-07-03 00:00:00
abstract：:Each homologous lobe of human serum transferrin (hTF) has one Fe(3+) ion bound by an aspartic acid, a histidine, two tyrosine residues, and two oxygens from the synergistic anion, carbonate. Extensive characterization of these ligands in the N-terminal lobe has been carried out. Despite sharing the same set of ligands...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050015f
更新日期：2005-06-07 00:00:00
abstract：:Histidine-40 is known to participate in phosphodiester transesterification catalyzed by the enzyme ribonuclease T1. A mutant enzyme with a lysine replacing the histidine-40 (His40Lys RNase T1) retains considerable catalytic activity [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-90...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00161a009
更新日期：1992-11-24 00:00:00
abstract：:Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00453a010
更新日期：1990-01-09 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) is a dimeric protein which induces formation of new blood vessels (angiogenesis) through binding to VEGF-receptor-2 tyrosine kinase (VEGFR2 TK) or KDR (kinase insert domain-containing receptor) on the surface of endothelial cells. Angiogenesis has been shown to be essential fo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981291f
更新日期：1998-11-24 00:00:00
abstract：:The processes of liposome solubilization and reconstitution were studied by using n-dodecyl octaethylene glycol monoether (C12E8). The solubilization of large unilamellar liposomes prepared by reverse-phase evaporation was systematically investigated by turbidity, 31P nuclear magnetic resonance, and centrifugation exp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00492a022
更新日期：1990-10-09 00:00:00
abstract：:FixL is a heme-based oxygen-sensing histidine kinase that induces the expression of nitrogen fixation genes under hypoxic conditions. Oxygen dissociation from heme iron in the sensor domain of FixL initiates protein conformational changes that are transmitted to the histidine kinase domain, activating autophosphorylat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00405
更新日期：2016-07-26 00:00:00
abstract：:The aminoacylase-1/metallopeptidase 20 (Acy1/M20) family features several l-aminoacylases useful in biocatalysis. Mammalian Acy1, in particular, has been applied in racemic resolution and reverse hydrolysis. Despite recent advances in our understanding of the active site architecture and functioning, determinants of A...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702156h
更新日期：2008-04-08 00:00:00
abstract：:The structural domains of human apolipoprotein(a) (apo(a)) in the lipoprotein(a) (Lp(a)) particle have been recently investigated by limited proteolysis [Huby, T., Doucet, C., Dieplinger, H., Chapman, J., & Thillet, J. (1994) Biochemistry 33, 3335-3341]. We have shown that apo(a) can be cleaved into two structural dom...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00022a011
更新日期：1995-06-06 00:00:00
abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00828
更新日期：2020-12-22 00:00:00
abstract：:By limited proteolysis of a type kappa immunoglobulin light chain (Oku) with clostripain, both the VL and CL fragments were obtained with a high yield. The unfolding and refolding by guanidine hydrochloride of light chain Oku and its VL and CL fragments were studied at pH 7.5 and 25 degrees C with circular dichroism a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00393a015
更新日期：1987-09-22 00:00:00
abstract：:By exposing photosystem II (PSII) samples to an incrementing number of excitation flashes at room temperature, followed by freezing, we could compare the Mn-derived multiline EPR signal from the S2 oxidation state as prepared by 1, 5, 10, and 25 flashes of light. While the S2 multiline signals exhibited by these sampl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026848c
更新日期：2003-03-11 00:00:00
abstract：:It is shown that the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool. It is made up of both monovinyl- and divinylprotochlorophyllide esters. Although the two tetrapyrroles exhibited similar emission maxima, they were distinguishable by their Soret excitatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00562a027
更新日期：1980-10-14 00:00:00
