Abstract：
:We have characterized the kinetics and substrate requirements of prenyl-flavin synthase from yeast. This enzyme catalyzes the addition of an isopentenyl unit to reduced flavin mononucleotide (FMN) to form an additional six-membered ring that bridges N5 and C6 of the flavin nucleus, thereby converting the flavin from a redox cofactor to one that supports the decarboxylation of aryl carboxylic acids. In contrast to bacterial enzymes, the yeast enzyme was found to use dimethylallyl pyrophosphate, rather than dimethylallyl phosphate, as the prenyl donor in the reaction. We developed a coupled assay for prenyl-flavin synthase activity in which turnover was linked to the activation of the prenyl-flavin-dependent enzyme, ferulic acid decarboxylase. The kinetics of the reaction are extremely slow: kcat = 12.2 ± 0.2 h-1, and KM for dimethylallyl pyrophosphate = 9.8 ± 0.7 μM. The KM for reduced FMN was too low to be accurately measured. The kinetics of reduced FMN consumption were studied under pre-steady state conditions. The reaction of FMN was described well by first-order kinetics with a kapp of 17.4 ± 1.1 h-1. These results indicate that a chemical step, most likely formation of the carbon-carbon bond between C6 of the flavin and the isopentenyl moiety, is substantially rate-determining in the reaction.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Arunrattanamook N,Marsh ENGdoi
10.1021/acs.biochem.7b01131subject
Has Abstractpub_date
2018-02-06 00:00:00pages
696-700issue
5eissn
0006-2960issn
1520-4995journal_volume
57pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00364a007
更新日期：1986-08-12 00:00:00
abstract：:Thyroperoxidase (TPO), a type I transmembrane heme containing glycoprotein, catalyzes iodide organification and thyroid hormone synthesis. One of the two main alternatively spliced forms of this enzyme, TPOzanelli, which is present in Graves's disease thyroid tissue, has a cytoplasmic domain completely modified. In th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0026324
更新日期：2001-02-27 00:00:00
abstract：:The existence of bacterial K(+)/H(+) antiporters that prevent the overaccumulation of potassium in the cytoplasm was predicted by Peter Mitchell almost 50 years ago. The importance of K(+)/H(+) antiport for bacterial physiology is widely recognized, but its molecular mechanisms remain underinvestigated. Here, we demon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi902173y
更新日期：2010-03-23 00:00:00
abstract：:The potency of cholesterol to affect the acyl chain order and dynamics of cardiolipin membranes in the liquid-crystalline state was monitored by steady-state and time-resolved fluorescence anisotropy as well as excited-state lifetime measurements with cis- and trans-parinaric acids as probes. Up to a cholesterol mole ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00357a054
更新日期：1986-05-06 00:00:00
abstract：:Although the binding of various forms of bleomycin to DNA has been studied extensively, the transient nature of the activated bleomycin species which ultimately attacks DNA has largely precluded direct examination of its physical interactions with DNA. In an attempt to map the minimum binding site required for this sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962957d
更新日期：1997-04-08 00:00:00
abstract：:The simplest carbapenem antibiotic, (5R)-carbapen-2-em-3-carboxylic acid, is biosynthesized from primary metabolites in Pectobacterium carotorova by the action of three enzymes, carboxymethylproline synthase (hereafter named CarB), carbapenam synthetase, and carbapenem synthase. CarB, a member of the crotonase superfa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0483662
更新日期：2004-12-21 00:00:00
abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a002
更新日期：1992-07-21 00:00:00
abstract：:The X-ray crystal structure of recombinant Desulfovibrio vulgaris rubrerythrin (Rbr) that was subjected to metal constitution first with zinc and then iron, yielding ZnS(4)Rbr, is reported. A [Zn(SCys)(4)] site with no iron and a diiron site with no appreciable zinc in ZnS(4)Rbr were confirmed by analysis of the anoma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0356193
更新日期：2004-03-23 00:00:00
abstract：:The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00426a014
更新日期：1988-12-27 00:00:00
abstract：:Ammonia binds directly to the oxygen-evolving complex of photosystem II (PSII) upon formation of the S2 intermediate, as evidenced by electron paramagnetic resonance spectroscopy. We explore the binding mode by using quantum mechanics/molecular mechanics methods and simulations of extended X-ray absorption fine struct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00974
更新日期：2015-09-29 00:00:00
abstract：:N-(1-Pyrene)maleimide is nonfluorescent in aqueous solution but forms strongly fluorescent adducts with sulfhydryl groups of organic compounds or proteins. The conjugation reactions of N-(1-pyrene)maleimide are relatively fast and can be monitored by the increase in fluorescence intensity of the pyrene chromophore. In...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00658a025
更新日期：1976-06-29 00:00:00
abstract：:The effect of RNA secondary structure on the kinetics of DNA synthesis catalyzed by HIV-1 RT was determined using a 66 nucleotide RNA template containing a stable 12 base pair hairpin structure. Prior to reaching the hairpin structure, the primer elongation by RT was fast and the kinetics of polymerization was not aff...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971217h
更新日期：1997-10-14 00:00:00
abstract：:alpha-Conotoxin GI is a snail toxin protein consisting of 13 amino acids cross-linked by 2 intramolecular disulfide bridges. This toxin is an antagonist of acetylcholine receptors. The native sequence has been synthesized, along with nine additional variants in which non-cysteine residues are replaced by alanine or th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00111a021
更新日期：1991-11-26 00:00:00
abstract：:Ketopantoate reductase (EC 1.1.1.169) catalyzes the NADPH-dependent reduction of alpha-ketopantoate to form D-(-)-pantoate in the pantothenate/coenzyme A biosynthetic pathway. The enzyme encoded by the panE gene from E. coli K12 was overexpressed and purified to homogeneity. The native enzyme exists in solution as a m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992676g
更新日期：2000-04-04 00:00:00
abstract：:The photosynthetic antenna of Chloroflexus aurantiacus includes bacteriochlorophyll (BChl) C740 and BChl a792, both of which occur in chlorosomes, and B808-866 (containing BChl a808 and BChl a866), which is membrane-located (subscripts refer to near-infrared absorption maxima in vivo). BChl a792 is thought to mediate ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00400a024
更新日期：1987-12-29 00:00:00
abstract：:The aldehyde oxidoreductase from Desulfovibrio gigas belongs to the family of molybdenum hydroxylases. Besides a molybdenum cofactor which constitutes their active site, these enzymes contain two [2Fe-2S](2+,1+) clusters which are believed to transfer the electrons provided by the substrate to an acceptor which is eit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0510025
更新日期：2005-08-30 00:00:00
abstract：:The structure of premature core enzyme, an obligatory intermediate in both in vivo and in vitro assembly of Escherichia coli DNA-dependent RNA polymerase, was compared with that of native core enzyme. Though this assembled but inactive form of core enzyme harbors the gross conformation similar to that of native enzyme...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00573a006
更新日期：1979-03-20 00:00:00
abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00028a009
更新日期：1995-07-18 00:00:00
abstract：:The interaction of the TAR RNA element of human immunodeficiency virus type 1 (HIV-1) with a 2'-O-methyl analogue of an RNA hairpin aptamer previously identified by in vitro selection [Ducongé, F., and Toulmé, J. J. (1999) RNA 5, 1605-1614] was characterized by UV-monitored thermal denaturation and surface plasmon res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025974d
更新日期：2002-10-08 00:00:00
abstract：:The interaction of Ca2+ and vanadate with fluorescein isothiocyanate (FITC) labeled sarcoplasmic reticulum (SR) Ca2+-ATPase has been studied by following the kinetics of changes in the reporter group fluorescence and equilibrium fluorescence levels. The vanadate species bound to the enzyme is clearly monomeric orthova...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a057
更新日期：1989-01-24 00:00:00
abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00036a035
更新日期：1995-09-12 00:00:00
abstract：:A molecular model has been built of the equine infectious anemia virus (EIAV) proteinase on the basis of the crystal structures of the related Rous sarcoma virus (RSV) and human immunodeficiency virus (HIV) proteinases. The 104 residue long EIAV proteinase has 30 identical and 11 similar amino acids compared to those ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a019
更新日期：1993-04-06 00:00:00
abstract：:New homo- and heterobifunctional cross-linking reagents have been synthesized. These reagents are based on ortho ester, acetal, and ketal functionalities that undergo acid-catalyzed dissociation but are base stable. The protein-reactive group in all the homobifunctional reagents is a maleimide group; the heterobifunct...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00432a023
更新日期：1989-03-21 00:00:00
abstract：:In Sphingomonas CHY-1, a single ring-hydroxylating dioxygenase is responsible for the initial attack of a range of polycyclic aromatic hydrocarbons (PAHs) composed of up to five rings. The components of this enzyme were separately purified and characterized. The oxygenase component (ht-PhnI) was shown to contain one R...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0611311
更新日期：2006-10-10 00:00:00
abstract：:We have used [(1)H-(15)N]-HSQC NMR to investigate the structural changes that occur in both serpin and proteinase in forming the kinetically trapped covalent protein-protein complex that is the basis for serpin inhibition of serine proteinases. By alternately using (15)N-alanine specifically-labeled alpha(1)-proteinas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010100x
更新日期：2001-05-29 00:00:00
abstract：:Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00004
更新日期：2018-03-20 00:00:00
abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00378a023
更新日期：1987-02-24 00:00:00
abstract：:The effect of calcium ion on the thermal stability of thermolysin has been investigated. The native protein undergoes an irreversible structural change and autolysis at high temperature. Analysis of the calcium ion dependence of the apparent melting temperature observed spectroscopically gives an apparent deltaH of -x...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00650a024
更新日期：1976-03-09 00:00:00
abstract：:The origin of the nuclear magnetic resonance (NMR)-measurable ATP in equilibrium Pi exchange and whether it can be used to determine net oxidative ATP synthesis rates in the intact myocardium were examined by detailed measurements of ATP in equilibrium Pi exchange rates in both directions as a function of the myocardi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00397a045
更新日期：1987-11-17 00:00:00
abstract：:The interaction of pig plasma gelsolin (G) and actin (A) was examined by using photoreactive 4-maleimidobenzophenone-actin (BPM-actin) in which BPM was previously conjugated to Cys-374 of actin through the maleimide moiety. In the presence of micromolar [Ca2+], the major cross-linked product observed after irradiation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00237a020
更新日期：1991-06-11 00:00:00
