Study of the spin-spin interactions between the metal centers of Desulfovibrio gigas aldehyde oxidoreductase: identification of the reducible sites of the [2Fe-2S]1+,2+ clusters.

abstract：:The interaction of bovine spleen profilin with ATP- and ADP-G-actin and poly(L-proline) has been studied by spectrofluorimetry, analytical ultracentrifugation, and rapid kinetics in low ionic strength buffer. Profilin binding to G-actin is accompanied by a large quenching of tryptophan fluorescence, allowing the measu...

journal_title：Biochemistry

authors： Perelroizen I,Marchand JB,Blanchoin L,Didry D,Carlier MF

更新日期：1994-07-19 00:00:00

abstract：:Molecular interactions between the tumor suppressor p53 and the transcriptional coactivators CBP/p300 are critical for the regulation of p53 transactivation and stability. The transactivation domain (TAD) of p53 binds directly to several CBP/p300 domains (TAZ1, TAZ2, NCBD, and KIX). Here we map the interaction between...

journal_title：Biochemistry

authors： Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-03-17 00:00:00

abstract：:The light chain of activated factor IX (FIXa) is involved in a number of functional properties, including FIXa enzymatic activity. This suggests the existence of a functional link between the FIXa light chain and the catalytic domain. The FIXa structure includes a few putative interactions between EGF2 and the proteas...

journal_title：Biochemistry

authors： Fribourg C,Meijer AB,Mertens K

更新日期：2006-09-05 00:00:00

abstract：:Initial rate kinetic studies and reduced coenzyme binding studies with bovine glutamate dehydrogenase have shown that an enzyme-NAD(P)H-glutamate abortive complex is a major participant in the overall enzyme-catalyzed reaction. The allosteric regulator ADP is shown to activate the enzyme by destabilizing this abortive...

journal_title：Biochemistry

authors： George A,Bell JE

更新日期：1980-12-23 00:00:00

abstract：:Mucopolysaccharides were isolated from calf cerebral microvasculature and calf aorta. The only complex carbohydrates that exhibited anticoagulant activity were heparin-like components. The biologic potencies of calf cerebral and aortic heparin-like species were 2.92 units/mg of anti-factor Xa activity and 2.85 units/m...

journal_title：Biochemistry

authors： Marcum JA,Rosenberg RD

更新日期：1984-04-10 00:00:00

abstract：:The structural consequences of specific base sequences in DNA can exert a strong influence on the function of DNA. It has previously been reported that the presence of multiple B-Z conformational junctions in constructed DNA oligomers results in unusually enhanced electrophoretic gel mobilities of these oligomers [Win...

journal_title：Biochemistry

authors： Lu M,Guo Q,Kallenbach NR,Sheardy RD

更新日期：1992-05-19 00:00:00

abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...

journal_title：Biochemistry

authors： Pi N,Armstrong JI,Bertozzi CR,Leary JA

更新日期：2002-11-05 00:00:00

abstract：:The protein composition, steady state and time-resolved fluorescence emission spectra were studied in solubilized and aggregated LHCII complexes, that were prepared according to two different isolation protocols: (1) by fractionation of cation-depleted thylakoid membranes using the non-ionic detergent Triton X-100 acc...

journal_title：Biochemistry

authors： Vasil'ev S,Irrgang KD,Schrötter T,Bergmann A,Eichler HJ,Renger G

更新日期：1997-06-17 00:00:00

abstract：:The protein tyrosine kinase p72syk readily phosphorylates hematopoietic linkage cell-specific protein p50/HS1 with high stoichiometry (up to 4 mol of Pi/mol of protein) and favorable kinetic constants (Km 77 nM, kcat 0.37 s-1), at sites that display the motif that is specifically recognized by the HS2 domains of Src t...

journal_title：Biochemistry

authors： Ruzzene M,Brunati AM,Marin O,Donella-Deana A,Pinna LA

更新日期：1996-04-23 00:00:00

abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...

journal_title：Biochemistry

authors： Petri V,Hsieh M,Jamison E,Brenowitz M

更新日期：1998-11-10 00:00:00

abstract：:Fibronectin is composed of multiple homologous repeats and contains many functional domains. Two major heparin-binding domains have previously been identified: the Hep I site near the amino terminus and the Hep II site near the carboxyl terminus. The Hep II site has been considered the high-affinity heparin-binding si...

journal_title：Biochemistry

authors： Lin H,Lal R,Clegg DO

更新日期：2000-03-28 00:00:00

abstract：:Earlier work indicates that the major DNA repair phosphodiesterase (PDE) in yeast cells is the well-characterized Apn1 protein. Apn1 demonstrates both Mg2+-independent PDE activity and Mg2+-independent class II apurinic/apyrimidinic (AP) endonuclease activity and represents greater than 90% of the activity detected in...

journal_title：Biochemistry

authors： Sander M,Ramotar D

更新日期：1997-05-20 00:00:00

abstract：:The generation of neutralizing antibodies by peptide immunization is dependent on achieving conformational compatibility between antibodies and native protein. Consequently, approaches are needed for developing conformational mimics of protein neutralization sites. We replace putative main-chain hydrogen bonds (NH -->...

journal_title：Biochemistry

authors： Cabezas E,Wang M,Parren PW,Stanfield RL,Satterthwait AC

更新日期：2000-11-28 00:00:00

abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...

journal_title：Biochemistry

authors： Wang X,Ge W,Zhang Y

更新日期：2019-01-29 00:00:00

abstract：:Chicken histone H4, labeled separately at Met-84 with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid and 5-(iodoacetamido)fluorescein, was reassociated with unlabeled histones H2A, H2B, and H3 and 146 base pairs of DNA to produce fluorescently labeled nucleosomes having physical characteristics virtually...

journal_title：Biochemistry

authors： Chung DG,Lewis PN

更新日期：1986-09-09 00:00:00

abstract：:According to earlier genetic experiments, a region within the N-terminal 50-100 amino acids may be important for the replication function of T antigen, the initiator protein of simian virus 40 (SV40). We have investigated this possibility using the T antigen related D2 protein in several biochemical assay systems. D2 ...

journal_title：Biochemistry

authors： Klausing K,Scheffner M,Scheidtmann KH,Stahl H,Knippers R

更新日期：1989-03-07 00:00:00

abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...

journal_title：Biochemistry

authors： Gross A,Hubbell WL

更新日期：2002-01-29 00:00:00

abstract：:The interaction between factor Xa and factor Va was investigated both in solution and in the presence of phospholipid vesicles with varying contents of phosphatidylserine. The binding parameters were inferred from the kinetics of prothrombin activation. Factor Xa and factor Va form in solution an equimolar complex wit...

journal_title：Biochemistry

authors： Lindhout T,Govers-Riemslag JW,van de Waart P,Hemker HC,Rosing J

更新日期：1982-10-26 00:00:00

abstract：:In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...

journal_title：Biochemistry

authors： Nixon CF,Lim SA,Sailer ZR,Zheludev IN,Gee CL,Kelch BA,Harms MJ,Marqusee S

更新日期：2021-01-26 00:00:00

abstract：:Cell-signaling peptides have been extensively used to transport functional molecules across the plasma membrane into living cells. These peptides consist of a hydrophobic sequence and a cationic nuclear localization sequence (NLS). It has been assumed that the hydrophobic region penetrates the hydrophobic lipid bilaye...

journal_title：Biochemistry

authors： Ramamoorthy A,Kandasamy SK,Lee DK,Kidambi S,Larson RG

更新日期：2007-01-30 00:00:00

abstract：:The propeptide binding/activation site on the vitamin K dependent carboxylase has been localized to a region of carboxylase between residues Arg +50 and Glu +225 by photoinactivation studies using [125I]tyrosyl-labeled benzoylphenylalanine (Bpa)-containing analogs of proFIX19, a peptide containing residues -18 to +1 o...

journal_title：Biochemistry

authors： Yamada M,Kuliopulos A,Nelson NP,Roth DA,Furie B,Furie BC,Walsh CT

更新日期：1995-01-17 00:00:00

abstract：:An equimolar mixture of DL-[alpha-2H]- and DL-[alpha-13C]mandelate, when incubated with mandelate racemase (EC 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. Similar...

journal_title：Biochemistry

authors： Sharp TR,Hegeman GD,Kenyon GL

更新日期：1977-03-22 00:00:00

abstract：:These investigations characterize the covalent binding of reactive products of prostaglandin H-synthases (PGHSs) to the enzyme and to other molecules. The intermediate product of oxygenation of arachidonic acid by the PGHSs, prostaglandin (PG) H2, undergoes rearrangement to the highly reactive gamma-keto aldehydes, le...

journal_title：Biochemistry

authors： Boutaud O,Brame CJ,Chaurand P,Li J,Rowlinson SW,Crews BC,Ji C,Marnett LJ,Caprioli RM,Roberts LJ 2nd,Oates JA

更新日期：2001-06-12 00:00:00

abstract：:Phospholamban (PLB) is a major target of the beta-adrenergic cascade in the heart, and functions to modulate rate-limiting conformational transitions involving the transport activity of the Ca-ATPase. To investigate structural changes within the Ca-ATPase that result from the phosphorylation of PLB by cAMP-dependent p...

journal_title：Biochemistry

authors： Negash S,Huang S,Squier TC

更新日期：1999-06-22 00:00:00

abstract：:Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyzes the first step in the biosynthesis of the branched-chain amino acids. In bacteria, the enzyme has a large subunit containing the catalytic machinery and a small subunit with a regulatory role. In eucaryotes, the evidence for a regulatory subunit is largely indire...

journal_title：Biochemistry

authors： Pang SS,Duggleby RG

更新日期：1999-04-20 00:00:00

abstract：:Heme-copper oxidases are membrane-bound proteins that catalyze the reduction of O(2) to H(2)O, a highly exergonic reaction. Part of the free energy of this reaction is used for pumping of protons across the membrane. The ba(3) oxidase from Thermus thermophilus presumably uses a single proton pathway for the transfer o...

journal_title：Biochemistry

authors： von Ballmoos C,Lachmann P,Gennis RB,Ädelroth P,Brzezinski P

更新日期：2012-06-05 00:00:00

abstract：:Nicotinic acetylcholine receptors (nAChRs) were studied in detail in the past regarding their interaction with therapeutic and drug addiction related compounds. Using fast kinetic whole-cell recording, we have now studied effects of tacrine, an agent used clinically to treat Alzheimer's disease, on currents elicited b...

journal_title：Biochemistry

authors： Cheffer A,Ulrich H

更新日期：2011-03-22 00:00:00

abstract：:The affinity of various carbohydrates for the galactose/N-acetylgalactosamine-specific lectin of the rabbit liver was assessed by determining the effect of these carbohydrates on the binding of [125I]asialoorosomucoid (125I-ASOR) by the lectin. To obtain the concentration of the inhibitor that causes 50% reduction in ...

journal_title：Biochemistry

authors： Lee RT,Myers RW,Lee YC

更新日期：1982-11-23 00:00:00

abstract：:Bax protein plays a key role in mitochondrial membrane permeabilization and cytochrome c release upon apoptosis. Our recent data have indicated that the 20-residue C-terminal peptide of Bax (BaxC-KK; VTIFVAGVLTASLTIWKKMG), when expressed intracellularly, translocates to the mitochondria and exerts lethal effect on can...

journal_title：Biochemistry

authors： Tatulian SA,Garg P,Nemec KN,Chen B,Khaled AR

更新日期：2012-11-20 00:00:00

abstract：:Characterization of the complete gene sequence encoding the alpha-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl hydrolase family 27 [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. This family, together with the family 36 alpha-galactosidases, forms g...

journal_title：Biochemistry

authors： Hart DO,He S,Chany CJ 2nd,Withers SG,Sims PF,Sinnott ML,Brumer H 3rd

更新日期：2000-08-15 00:00:00