Inhibition mechanism of rat α₃β₄ nicotinic acetylcholine receptor by the Alzheimer therapeutic tacrine.

abstract：:Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...

journal_title：Biochemistry

authors： Lewis RW,Mabry J,Polisar JG,Eagen KP,Ganem B,Hess GP

更新日期：2010-06-15 00:00:00

abstract：:The reduction of CBrCl3 by the heme-heme oxygenase complex forms dissociable and covalently bound heme products. No such products are formed with mesoheme in which the heme vinyl substituents are replaced by ethyl groups. The dissociable heme products are chromatographically similar but not identical to those obtained...

journal_title：Biochemistry

authors： Wilks A,Medzihradszky KF,Ortiz de Montellano PR

更新日期：1998-03-03 00:00:00

abstract：:Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...

journal_title：Biochemistry

authors： Pak ML,Lakowski TM,Thomas D,Vhuiyan MI,Hüsecken K,Frankel A

更新日期：2011-09-27 00:00:00

abstract：:Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...

journal_title：Biochemistry

authors： Dalbey RE,Wells JA,Yount RG

更新日期：1983-01-18 00:00:00

abstract：:Myotoxin a from the venom of Crotalus viridis viridis (prairie rattlesnake) is a small protein which is responsible for myonecrosis. It is a basic protein with 42 amino acid residues of known sequence. Three disulfide bonds give it a highly compact structure. Microscopic examination of the toxin's effects reveals that...

journal_title：Biochemistry

authors： Liddle WK,Tu AT

更新日期：1985-12-17 00:00:00

abstract：:A new fluorescence method to measure viscosity in cell cytosol [Fushimi, K., & Verkman, A. S. (1991) J. Cell Biol. 112, 719-725] has been applied to determine fluid-phase viscosity in sea urchin eggs. Freshly harvested eggs from Lytechinus pictus were loaded with the dyes 2,7-bis(2-carboxyethyl)-5-(and-6-)carboxyfluor...

journal_title：Biochemistry

authors： Periasamy N,Armijo M,Verkman AS

更新日期：1991-12-24 00:00:00

abstract：:In the putative secondary structure of the transposon Tn10-encoded metal-tetracycline/H+ antiporter [Yamaguchi, et al. (1992) J. Biol. Chem. 267, 7490-7498], Tyr50-XXX-Gln54 in transmembrane helix 2 and Gly80-XXX-Asp84 in helix 3 are thought to face each other in the N-terminal region. At the corresponding positions i...

journal_title：Biochemistry

authors： Yamaguchi A,Akasaka T,Kimura T,Sakai T,Adachi Y,Sawai T

更新日期：1993-06-01 00:00:00

abstract：:The human pathogen Staphylococcus aureus acquires heme iron from hemoglobin (Hb) via the action of a series of iron-regulated surface determinant (Isd) proteins. The cell wall anchored IsdB protein is recognized as the predominant Hb receptor, and is comprised of two NEAr transporter (NEAT) domains that act in concert...

journal_title：Biochemistry

authors： Fonner BA,Tripet BP,Eilers BJ,Stanisich J,Sullivan-Springhetti RK,Moore R,Liu M,Lei B,Copié V

更新日期：2014-06-24 00:00:00

abstract：:Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...

journal_title：Biochemistry

authors： Tyagi NK,Goyal P,Kumar A,Pandey D,Siess W,Kinne RK

更新日期：2005-11-29 00:00:00

abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...

journal_title：Biochemistry

authors： Makde RD,Mahajan SK,Kumar V

更新日期：2007-02-27 00:00:00

abstract：:Oxalate:ferredoxin oxidoreductase (OOR) is an unusual member of the thiamine pyrophosphate (TPP)-dependent 2-oxoacid:ferredoxin oxidoreductase (OFOR) family in that it catalyzes the coenzyme A (CoA)-independent conversion of oxalate into 2 equivalents of carbon dioxide. This reaction is surprising because binding of C...

journal_title：Biochemistry

authors： Pierce E,Mansoorabadi SO,Can M,Reed GH,Ragsdale SW

更新日期：2017-06-06 00:00:00

abstract：:Adenylosuccinate lyase of Bacillus subtilis is inactivated by 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 5'-monophosphate (2-BDB-TAMP) at pH 7.0. As the reagent concentration is increased, a maximum rate constant is approached, indicative of reversible enzyme-reagent complex formation (KR = 68 +/- 9 microM) prior to ir...

journal_title：Biochemistry

authors： Lee TT,Worby C,Bao ZQ,Dixon JE,Colman RF

更新日期：1998-06-09 00:00:00

abstract：:The complete primary and three-dimensional solution structures of subtilosin A (1), a bacteriocin from Bacillus subtilis, were determined by multidimensional NMR studies on peptide produced using isotopically labeled [(13)C,(15)N]medium derived from Anabaena sp. grown on sodium [(13)C]bicarbonate and [(15)N]nitrate. A...

journal_title：Biochemistry

authors： Kawulka KE,Sprules T,Diaper CM,Whittal RM,McKay RT,Mercier P,Zuber P,Vederas JC

更新日期：2004-03-30 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:The initial hydrolysis of CaATP by chloroplast coupling factor 1 was studied with the quenched-flow method. The time course of hydrolysis can be described as a first-order conversion of the enzyme to an active form followed by steady-state formation of product. The rat constant for the first-order process is independe...

journal_title：Biochemistry

authors： Leckband D,Hammes GG

更新日期：1987-04-21 00:00:00

abstract：:The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...

journal_title：Biochemistry

authors： Laughrea M,Jetté L

更新日期：1996-02-06 00:00:00

abstract：:The active form of the animal fatty acid synthase (FAS) is a dimer of identical multifunctional polypeptides, each containing seven discrete functional domains, that cooperate to form two centers for palmitate synthesis. To assess the importance of domain cooperation across the subunit interface in the reaction mechan...

journal_title：Biochemistry

authors： Joshi AK,Witkowski A,Smith S

更新日期：1998-02-24 00:00:00

abstract：:Antifreeze proteins lower the freezing point of their solution by binding to ice and inhibiting its growth. One of several structurally different antifreeze proteins in fishes (type II) is homologous to the carbohydrate-recognition domain of Ca2+-dependent lectins and adopts the same three-dimensional fold. Type II an...

journal_title：Biochemistry

authors： Loewen MC,Gronwald W,Sönnichsen FD,Sykes BD,Davies PL

更新日期：1998-12-22 00:00:00

abstract：:The mitogen-activated protein kinases (MAPKs) are key components of cellular signal transduction pathways, which are down-regulated by the MAPK phosphatases (MKPs). Catalytic activity of the MKPs is controlled both by their ability to recognize selective MAPKs and by allosteric activation upon binding to MAPK substrat...

journal_title：Biochemistry

authors： Lu C,Liu X,Zhang CS,Gong H,Wu JW,Wang ZX

更新日期：2017-11-21 00:00:00

abstract：:The fatty acid acylated insulin, Lys(B29)-tetradecanoyl, des-(B30) human insulin, has been crystallized and the structure determined by X-ray crystallography. The fatty acid substituent on residue B29 Lys binds reversibly to circulating albumin protein in vivo, and by this mechanism the hormone's action is prolonged. ...

journal_title：Biochemistry

authors： Whittingham JL,Havelund S,Jonassen I

更新日期：1997-03-11 00:00:00

abstract：:The molecular structure of the T4 phage tail sheath protein, gp18, was studied by limited proteolysis, immunoblotting, and immunoelectron microscopy. Gp18 is extremely resistant to proteolysis in the assembled form of either extended or contracted sheaths, but it is readily cleaved by proteases in the monomeric form, ...

journal_title：Biochemistry

authors： Arisaka F,Takeda S,Funane K,Nishijima N,Ishii S

更新日期：1990-05-29 00:00:00

abstract：:Transient phosphorylation at an aspartate residue on the Spo0F protein is a central step in the phosphorelay signal transduction pathway controlling sporulation in Bacilli. The response regulator Spo0F-P is stable to hydrolysis (t1/2 > 24 h at 23 degrees C in the absence of Mg2+), allowing the use of nondenaturing PAG...

journal_title：Biochemistry

authors： Zapf JW,Hoch JA,Whiteley JM

更新日期：1996-03-05 00:00:00

abstract：:In Escherichia coli, the PurT-encoded glycinamide ribonucleotide transformylase, or PurT transformylase, catalyzes an alternative formylation of glycinamide ribonucleotide (GAR) in the de novo pathway for purine biosynthesis. On the basis of amino acid sequence analyses, it is known that the PurT transformylase belong...

journal_title：Biochemistry

authors： Thoden JB,Firestine S,Nixon A,Benkovic SJ,Holden HM

更新日期：2000-08-01 00:00:00

abstract：:X-ray absorption spectroscopy has been performed on oriented photosystem II membrane particles isolated from spinach. Structural features of the tetranuclear Mn cluster and the orientation of the cluster with respect to the lipid bilayer were determined in both the S1 and S2 states of the Kok cycle. Variation of the s...

journal_title：Biochemistry

authors： Mukerji I,Andrews JC,DeRose VJ,Latimer MJ,Yachandra VK,Sauer K,Klein MP

更新日期：1994-08-16 00:00:00

abstract：:Formiminotransferase-cyclodeaminase denatured in 6 M guanidine hydrochloride (Gdn.HCl) refolds and reassembles to the native octameric structure upon dilution into buffer. Both enzymic activities are recovered to greater than 90%, and the renatured enzyme "channels" the formiminotetrahydropteroylpentaglutamate interme...

journal_title：Biochemistry

authors： Findlay WA,MacKenzie RE

更新日期：1988-05-03 00:00:00

abstract：:A subgroup of enzymes in the NAD(P)H:FMN reductase family is comprised of flavin reductases from two-component monooxygenase systems. The diverging structural feature in these FMN reductases is a π-helix centrally located at the tetramer interface that is generated by the insertion of an amino acid in a conserved α4 h...

journal_title：Biochemistry

authors： Musila JM,L Forbes D,Ellis HR

更新日期：2018-07-31 00:00:00

abstract：:Insulin and insulin-like growth factor 1 (IGF-1) share high sequence homology, but their folding behaviors are significantly different: insulin folds into one unique thermodynamically controlled structure, while IGF-1 folds into two thermodynamically controlled disulfide isomers. However, the origin of their different...

journal_title：Biochemistry

authors： Wang S,Guo ZY,Shen L,Zhang YJ,Feng YM

更新日期：2003-08-19 00:00:00

abstract：:The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...

journal_title：Biochemistry

authors： Hyland LJ,Tomaszek TA Jr,Roberts GD,Carr SA,Magaard VW,Bryan HL,Fakhoury SA,Moore ML,Minnich MD,Culp JS

更新日期：1991-08-27 00:00:00

abstract：:Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...

journal_title：Biochemistry

authors： Allawi HT,SantaLucia J Jr

更新日期：1997-08-26 00:00:00

abstract：:Drug substrates stimulate ATPase activity of the P-glycoprotein (P-gp) ATP-binding cassette drug pump by an unknown mechanism. Cross-linking analysis was performed to test if drug substrates stimulate P-gp ATPase activity by altering cross-talk at the first transmission interface linking the drug-binding [intracellula...

journal_title：Biochemistry

authors： Loo TW,Clarke DM

更新日期：2016-05-24 00:00:00