Crystal structure of a prolonged-acting insulin with albumin-binding properties.

abstract：:Previously we showed that the low-molecular-weight mucin (MG2, encoded by MUC7), a major component of human submandibular/sublingual saliva, is a bacterial receptor that coats the tooth surface. Here we tested the hypothesis that the structure of its carbohydrate residues contains important information about its funct...

journal_title：Biochemistry

authors： Prakobphol A,Thomsson KA,Hansson GC,Rosen SD,Singer MS,Phillips NJ,Medzihradszky KF,Burlingame AL,Leffler H,Fisher SJ

更新日期：1998-04-07 00:00:00

abstract：:Polyamides composed of N-methylpyrrole (Py) and N-methylimidazole (Im) subunits can bind in the minor groove of DNA at predetermined sequences with subnanomolar affinity and high specificity. Covalent linkage of polymer subunits using a gamma-aminobutyric acid linker has been shown to increase both the affinity and sp...

journal_title：Biochemistry

authors： Baliga R,Baird EE,Herman DM,Melander C,Dervan PB,Crothers DM

更新日期：2001-01-09 00:00:00

abstract：:Light-driven outward sodium-pumping rhodopsin (NaR) was recently found in marine bacteria. Krokinobacter eikastus rhodopsin 2 (KR2) actively transports sodium and lithium ions in NaCl and LiCl, respectively, while it pumps protons in KCl. NaR has a conserved NDQ (N112, D116, and Q123 in KR2) motif, and previous studie...

journal_title：Biochemistry

authors： Abe-Yoshizumi R,Inoue K,Kato HE,Nureki O,Kandori H

更新日期：2016-10-18 00:00:00

abstract：:Structural features of a 19-nucleotide RNA hairpin loop (5'-GGCGUACGUUUCGUACGCC-3'), a loop motif which occurs in eukaryotic 18S rRNA, have been derived using multidimensional heteronuclear NMR spectroscopy in combination with local conformational analysis and torsion angle distance geometry followed by restrained ene...

journal_title：Biochemistry

authors： Sich C,Ohlenschläger O,Ramachandran R,Görlach M,Brown LR

更新日期：1997-11-18 00:00:00

abstract：:Conjugative transfer of F-like plasmids in Escherichia coli is repressed by a plasmid-encoded protein, FinO. FinO blocks the translation of TraJ, a positive activator of transcription of genes required for conjugation. FinO binds a traJ antisense RNA, FinP, thereby protecting it from degradation, and catalyzes FinP-tr...

journal_title：Biochemistry

authors： Ghetu AF,Gubbins MJ,Oikawa K,Kay CM,Frost LS,Glover JN

更新日期：1999-10-19 00:00:00

abstract：:Sedimentation and gel retardation studies show a stronger interaction of HMG 1 and 2 with negatively supercoiled DNA than with linear, nicked-circular, or positively supercoiled ds-DNA. An apparent unwinding angle of 58 degrees was obtained for HMG 1 and 2 when assayed by protection of negatively supercoiled DNA from ...

journal_title：Biochemistry

authors： Sheflin LG,Fucile NW,Spaulding SW

更新日期：1993-04-06 00:00:00

abstract：:Although the binding of various forms of bleomycin to DNA has been studied extensively, the transient nature of the activated bleomycin species which ultimately attacks DNA has largely precluded direct examination of its physical interactions with DNA. In an attempt to map the minimum binding site required for this sp...

journal_title：Biochemistry

authors： Suh D,Povirk LF

更新日期：1997-04-08 00:00:00

abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...

journal_title：Biochemistry

authors： Ziehler WA,Day JJ,Fierke CA,Engelke DR

更新日期：2000-08-15 00:00:00

abstract：:Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...

journal_title：Biochemistry

authors： Zhang X,Adda CG,Low A,Zhang J,Zhang W,Sun H,Tu X,Anders RF,Norton RS

更新日期：2012-02-21 00:00:00

abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

journal_title：Biochemistry

authors： Robbins AR,Baker RM

更新日期：1977-11-15 00:00:00

abstract：:Complex I, a key component of the mitochondrial respiratory chain, exhibits diminished activity as a result of cardiac ischemia/reperfusion. Cardiac ischemia/reperfusion is associated with increases in the levels of mitochondrial Ca(2+) and pro-oxidants. In the current in vitro study, we sought evidence for a mechanis...

journal_title：Biochemistry

authors： Sadek HA,Szweda PA,Szweda LI

更新日期：2004-07-06 00:00:00

abstract：:The proteasome inhibitors, lactacystin and N-acetyl-leucyl-leucyl-norlucinal, caused a rapid and near-complete loss of approximately 22-23-kDa ubiquitinated nucleoproteins, which we have identified as monoubiquitinated nucleosomal histones H2A and H2B by immunological and two-dimensional electrophoretic techniques. In...

journal_title：Biochemistry

authors： Mimnaugh EG,Chen HY,Davie JR,Celis JE,Neckers L

更新日期：1997-11-25 00:00:00

abstract：:Six structural repeat motifs of 58 amino acids are found in the sequence of both mouse and human properdins. Twelve more examples of the motif are available from the sequences of thrombospondin, the terminal complement components, and the thrombospondin-related anonymous protein. The averaged Robson and Chou-Fasman se...

journal_title：Biochemistry

authors： Perkins SJ,Nealis AS,Haris PI,Chapman D,Goundis D,Reid KB

更新日期：1989-09-05 00:00:00

abstract：:We report the use of anti-nucleoside antibodies to probe for local denaturation of calf thymus DNA upon binding of the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, and the biologically inactive analogues trans-diamminedichloroplatinum(II), trans-DDP, and chloro(diethylenetriamine)platinum(II) chloride, [P...

journal_title：Biochemistry

authors： Sundquist WI,Lippard SJ,Stollar BD

更新日期：1986-04-08 00:00:00

abstract：:Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5' terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) ribonucleic acid (RNA) to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat gern 80S ribosomes convey partial p...

journal_title：Biochemistry

authors： Browning KS,Leung DW,Clark JM Jr

更新日期：1980-05-13 00:00:00

abstract：:The protease prostate-specific antigen (PSA) is a marker widely used clinically for monitoring prostatic malignancies. Under normal conditions, this enzyme is mainly involved in the post ejaculation degradation of the major human seminal protein, the seminal plasma motility inhibitor precursor/semenogelin I (SPMIP/SgI...

journal_title：Biochemistry

authors： Robert M,Gibbs BF,Jacobson E,Gagnon C

更新日期：1997-04-01 00:00:00

abstract：:Resonance energy-transfer approaches have been used to directly monitor the interactions of the GTP gamma S-bound alpha subunit of transducin (alpha T GTP gamma S) with the retinal cyclic GMP phosphodiesterase (PDE). The PDE was labeled with 5-(iodoacetamido) fluorescein (IAF-PDE) and served as the fluorescence donor ...

journal_title：Biochemistry

authors： Erickson JW,Cerione RA

更新日期：1991-07-23 00:00:00

abstract：:L6 is a monoclonal antitumor antibody which recognizes an epitope located in a 42-residue extracellular domain of a tumor-associated approximately 22 kDa glycoprotein antigen. The L6 mAb localizes to solid tumors in vivo and triggers complement activation and antibody-dependent cellular cytotoxicity. It has been the s...

journal_title：Biochemistry

authors： Stenzel-Johnson PR,Yelton D,Bajorath J

更新日期：1994-12-06 00:00:00

abstract：:Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare [N epsilon, N epsilon-C2H3]lysyl cytochrome c, and deuterium exchange provided labeling of backbone sites to give [amide-2H]cytochrome c or mo...

journal_title：Biochemistry

authors： Spooner PJ,Watts A

更新日期：1991-04-23 00:00:00

abstract：:Multidimensional heteronuclear NMR techniques were applied to study the phosphotransfer domain, residues 1-134, of the histidine kinase CheA, from Escherichia coli, which contains the site of autophosphorylation, His48. Assignments of the backbone amide groups and side chain protons are nearly complete. Our studies sh...

journal_title：Biochemistry

authors： Zhou H,Lowry DF,Swanson RV,Simon MI,Dahlquist FW

更新日期：1995-10-24 00:00:00

abstract：:Cytochrome P450 3A4 is a drug-metabolizing enzyme of extraordinarily broad substrate specificity. This quality imparts upon the enzyme special importance in understanding its determinants of activity and substrate recognition. Limited successes in P450 3A4 active-site structure studies have been achieved by use of mec...

journal_title：Biochemistry

authors： Gartner CA,Wen B,Wan J,Becker RS,Jones G 2nd,Gygi SP,Nelson SD

更新日期：2005-02-15 00:00:00

abstract：:The sliding clamp of the Escherichia coli replisome is now understood to interact with many proteins involved in DNA synthesis and repair. A universal interaction motif is proposed to be one mechanism by which those proteins bind the E. coli sliding clamp, a homodimer of the beta subunit, at a single site on the dimer...

journal_title：Biochemistry

authors： Wijffels G,Dalrymple BP,Prosselkov P,Kongsuwan K,Epa VC,Lilley PE,Jergic S,Buchardt J,Brown SE,Alewood PF,Jennings PA,Dixon NE

更新日期：2004-05-18 00:00:00

abstract：:Covalent labeling of the canine renal parathyroid hormone receptor with [125I]bPTH(1-34) reveals several major binding components that display characteristics consistent with a physiologically relevant adenylate cyclase linked receptor. Through the use of the specific glycosidases neuraminidase and endoglycosidase F a...

journal_title：Biochemistry

authors： Karpf DB,Arnaud CD,King K,Bambino T,Winer J,Nyiredy K,Nissenson RA

更新日期：1987-12-01 00:00:00

abstract：:Galectin-3 is a member of a large family of beta-galactoside-binding animal lectins and is composed of a carboxyl-terminal lectin domain connected to an amino-terminal nonlectin part. Previous experimental results suggest that, when bound to multivalent glycoconjugates, galectin-3 self-associates through intermolecula...

journal_title：Biochemistry

authors： Yang RY,Hill PN,Hsu DK,Liu FT

更新日期：1998-03-24 00:00:00

abstract：:The binding of propidium to poly(dA).poly(dT) [poly(dA.dT)] and to poly[d(A-T)].poly[d(A-T)] [poly[d(A-T)2]] has been compared under a variety of solution conditions by viscometric titrations, binding studies, and kinetic experiments. The binding of propidium to poly[d(A-T)2] is quite similar to its binding to calf th...

journal_title：Biochemistry

authors： Wilson WD,Wang YH,Krishnamoorthy CR,Smith JC

更新日期：1985-07-16 00:00:00

abstract：:The AT-rich interaction domain (ARID) containing BAF250a is a subunit of the BAF-A class of SWI/SNF chromatin remodeling complexes. The ARID belongs to a family of conserved DNA binding domains found in several eukaryotic proteins; however, its exact contribution to BAF250a function and the mechanism of its DNA bindin...

journal_title：Biochemistry

authors： Giri M,Maulik A,Singh M

更新日期：2020-01-14 00:00:00

abstract：:Currently, the relatively high cost of enzymes such as glycoside hydrolases that catalyze cellulose hydrolysis represents a barrier to commercialization of a biorefinery capable of producing renewable transportable fuels such as ethanol from abundant lignocellulosic biomass. Among the many families of glycoside hydrol...

journal_title：Biochemistry

authors： Harris PV,Welner D,McFarland KC,Re E,Navarro Poulsen JC,Brown K,Salbo R,Ding H,Vlasenko E,Merino S,Xu F,Cherry J,Larsen S,Lo Leggio L

更新日期：2010-04-20 00:00:00

abstract：:p-Cresol methylhydroxylase (PCMH) isolated from Pseudomonas putida is an alpha 2 beta 2 tetramer of approximate subunit Mr 49,000 and 9,000. It is a flavocytochrome c containing covalently bound FAD in the larger subunit and covalently bound heme in the smaller. Crystals in space group P2(1)2(1)2(1) with unit-cell par...

journal_title：Biochemistry

authors： Mathews FS,Chen ZW,Bellamy HD,McIntire WS

更新日期：1991-01-08 00:00:00

abstract：:Nramp (natural resistance-associated macrophage protein) family members have been characterized in mammals, yeast, and bacteria as divalent metal ion/H(+) symporters. In previous work, a bioinformatic approach was used for the identification of residues that are conserved within the Nramp family [Haemig, H. A., and Br...

journal_title：Biochemistry

authors： Haemig HA,Moen PJ,Brooker RJ

更新日期：2010-06-08 00:00:00

abstract：:Photosensitized oxidation reactions damage tissue by catalyzing the formation of oxyradicals and singlet oxygen. beta-Carotene is hypothesized to exert photoprotective effects by quenching singlet oxygen formed by Type II reactions and by scavenging free radicals formed by Type I reactions. beta-Carotene antioxidant m...

journal_title：Biochemistry

authors： Stratton SP,Liebler DC

更新日期：1997-10-21 00:00:00