Abstract：
:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell proliferation. To better understand the molecular mechanism for DHX33 in exerting its biological functions, we purified recombinant DHX33 and performed biochemical studies in vitro. DHX33 protein was found to have ATPase activity that is dependent on DNA or RNA duplexes. The ATPase activity of DHX33 is coupled with its RNA/DNA unwinding activity. If a key residue in the ATP binding site were mutated, the mutant DHX33 could not unwind DNA/RNA duplexes. Furthermore, a deletion mutant of a RKK motif previously identified to be involved in ribosome DNA binding could still unwind DNA duplexes, albeit with reduced efficiency. In summary, our study reveals that purified DHX33 protein possesses unwinding activity toward DNA and RNA duplexes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wang X,Ge W,Zhang Ydoi
10.1021/acs.biochem.8b00166subject
Has Abstractpub_date
2019-01-29 00:00:00pages
250-258issue
4eissn
0006-2960issn
1520-4995journal_volume
58pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Based on the atomic structures of the mitochondrial cytochrome bc(1) complex, it has been proposed that the soluble domain of the [2Fe-2S] Rieske iron-sulfur protein (ISP) must rotate by ca. 60 degrees and translate through an appreciable distance between two binding sites, proximal to cytochrome c(1) and to the lumen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9921992
更新日期：2000-03-14 00:00:00
abstract：:The kinetic parameters (kpol, Kd app) for all possible correct and incorrect pairing between the A, T, G, and C bases were determined for wild-type (WT) rat DNA polymerase beta (pol beta) and the R283A mutant under pre-steady-state kinetic assay conditions. The base substitution fidelities of these two proteins were t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961653o
更新日期：1997-02-04 00:00:00
abstract：:Site-directed mutants of human carbonic anhydrase III were used to examine the role of Thr-199 and its interaction with Phe-198 in the catalyzed hydration of CO2. Threonine-199 is a hydrogen bond acceptor for the zinc-bound water, and Phe-198 forms part of the hydrophobic side of the active-site cavity of carbonic anh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a004
更新日期：1993-08-10 00:00:00
abstract：:We have investigated the interaction of a number of synthetic 20-residue peptides, corresponding to the HA2 N-terminus of the influenza virus hemagglutinin (X31 strain), with phospholipid vesicles and monolayers. Besides the wild-type sequence, two peptides were studied with mutations corresponding to those previously...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00106a020
更新日期：1991-10-22 00:00:00
abstract：:Beta-lactams exert their antibiotic action through their inhibition of bacterial DD-peptidases (penicillin-binding proteins). Bacteria, in general, carry several such enzymes localized on the outside of their cell membrane to catalyze the final step in cell wall (peptidoglycan) synthesis. They have been classified int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061804f
更新日期：2006-12-26 00:00:00
abstract：:We present spectroscopic evidence for the presence of low-barrier hydrogen bonds (LBHBs) in molecular complexes composed of carboxylic acids and 1-methylimidazole (1-MeIm) dissolved in aprotic organic solvents. A plot of the values of the low-field proton NMR chemical shifts versus the aqueous pKa of the carboxylic ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a002
更新日期：1995-05-30 00:00:00
abstract：:We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00261a031
更新日期：1982-08-31 00:00:00
abstract：:The O-linked protein glycosylation pathway in Neisseria gonorrhoeae is responsible for the synthesis of a complex oligosaccharide on undecaprenyl diphosphate and subsequent en bloc transfer of the glycan to serine residues of select periplasmic proteins. Protein glycosylation (pgl) genes have been annotated on the bas...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2003372
更新日期：2011-06-07 00:00:00
abstract：:The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00660a033
更新日期：1976-07-27 00:00:00
abstract：:Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9008402
更新日期：2009-09-08 00:00:00
abstract：:4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolysis of 4-CBA-CoA to 4-hydroxybenzoyl-coenzyme A (4-HBA-CoA) via a nucleophilic aromatic substitution pathway involving the participation of an active site carboxylate side chain in covalent catalysis. In this paper we report on the identification...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9609533
更新日期：1996-08-20 00:00:00
abstract：:The role of basic residues of the 70-80-loop, Arg(74), Arg(75), and Lys(78) (chymotrypsin numbering) in the catalytic function of activated protein C (APC) was investigated by expressing mutants of protein C in which these residues were replaced with Ala in three separate constructs. Following purification to homogene...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015899r
更新日期：2002-05-14 00:00:00
abstract：:We have recently reported the cation-induced self-assembly of DNA oligomers of the general sequence C4T4G4T1-4G4 into high-molecular weight multistranded structures [Marotta, S.P., Tamburri, P.A., and Sheardy, R.D. (1996) Biochemistry 35, 10484-10492]. The architecture of the proposed structure consists of a series of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi973009v
更新日期：1998-03-03 00:00:00
abstract：:The unfolding and dissociation of the tetrameric enzyme fructose-1,6-bisphosphatase from pig kidney by guanidine hydrochloride have been investigated at equilibrium by monitoring enzyme activity, ANS binding, intrinsic (tyrosine) protein fluorescence, exposure of thiol groups, fluorescence of extrinsic probes (AEDANS,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034203m
更新日期：2003-06-17 00:00:00
abstract：:Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational ensemble of the tandemly repeating tetrapeptide unit of the circumsporozoite coat protein of the malaria parasite Plasmodium falciparum. Peptides based on the Asn-Ala-Asn-Pro and Asn-Pro-Asn-Ala...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00486a008
更新日期：1990-08-28 00:00:00
abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300134b
更新日期：2012-04-03 00:00:00
abstract：:Seven hydrophobic residues ranging in size from glycine to phenylalanine have been substituted for the wild-type methionine residue at position 13 in a 15-residue truncated version (S15) of S-peptide, the small component of ribonuclease S. Complexes of both S-15 and the seven variants with S-protein yielded isomorphou...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00164a005
更新日期：1992-12-15 00:00:00
abstract：:Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7006959
更新日期：2007-07-31 00:00:00
abstract：:A soluble Ca2+/calmodulin-dependent protein kinase has been purified from rat brain to near homogeneity by using casein as substrate. The enzyme was purified by using hydroxylapatite adsorption chromatography, phosphocellulose ion-exchange chromatography, Sepharose 6B gel filtration, affinity chromatography using calm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00318a018
更新日期：1984-11-06 00:00:00
abstract：:Transcarboxylase (TC) is a multisubunit enzyme that catalyzes the transfer of a carboxylate group from methylmalonyl-CoA (MMCoA) to pyruvate. The CO2- group is shuttled between the MMCoA and pyruvate binding sites by a biotin cofactor, covalently linked to the 1.3S subunit. Fully carboxylated 1.3S can be prepared in v...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0116442
更新日期：2002-02-19 00:00:00
abstract：:The sex steroid binding protein (SBP) of human pregnancy serum was purified to homogeneity by the sequential use of ammonium sulfate precipitation, affinity chromatography on 5alpha-dihydrotestosterone-17beta-succinyldiaminoethyl-(1,4-butanediol diglycidyl ether)-agarose, and preparative polyacrylamide gel electrophor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00601a008
更新日期：1978-04-18 00:00:00
abstract：:Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00714
更新日期：2021-01-13 00:00:00
abstract：:Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00389a041
更新日期：1987-07-28 00:00:00
abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01298
更新日期：2019-03-05 00:00:00
abstract：:Prosthetic groups such as heme, chlorophyll, and cobalamin (vitamin B(12)) are characterized by their branched biosynthetic pathway and unique metal insertion steps. The metal ion chelatases can be broadly classed either as single-subunit ATP-independent enzymes, such as the anaerobic cobalt chelatase and the protopor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9906773
更新日期：1999-08-17 00:00:00
abstract：:A number of isomeric diacylglycerophosphocholines and diacylglycero sulfates containing O-acyl and/or S-acyl ester bonds were investigated as substrates for porcine pancreatic phospholipase A2 and its zymogen. A comparison is made with the kinetic properties of the enzyme toward the corresponding glycol detergents pre...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00348a023
更新日期：1985-12-31 00:00:00
abstract：:In search of the regulation mechanisms for isoform specific myosin assembly, we have used the COOH-terminal fragments of nonmuscle myosin isoforms MIIA and MIIB (MIIA(F46) and MIIB(alpha)(F47)) as a model system. Phosphorylation by protein kinase C (PK C) or casein kinase II (CK II) within or near the nonhelical tail-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000347e
更新日期：2000-09-19 00:00:00
abstract：:The proton nuclear magnetic resonance spectrum of human adult deoxyhemoglobin in D2O in the region from 6 to 20 ppm downfield from the proton resonance of residual water shows a number of hyperfine shifted proton resonances that are due to groups on or near the alpha and beta hemes. The sensitivity of these resonances...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00590a031
更新日期：1979-11-13 00:00:00
abstract：:The mechanism of wild-type and R37A mutant Pseudomonas dacunhae aspartate beta-decarboxylase (ABDC) was studied by rapid-scanning stopped-flow spectrophotometry. Mixing wild-type ABDC with 50 mM disodium l-Asp resulted in the formation of a 325 nm absorption peak within the dead time of the stopped-flow instrument, li...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100272g
更新日期：2010-06-22 00:00:00
abstract：:Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047936v
更新日期：2005-03-08 00:00:00
