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Composite organization of the cobalamin binding and cubilin recognition sites of intrinsic factor.

Abstract：

:Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cbl) = 4 x 10(6), 14 x 10(6), and 26 x 10(6) M(-1) s(-1), respectively), but the equilibrium dissociation constants were essentially different (K(Cbl) = 200 microM, 0.2 microM, and

journal_name

Biochemistry

journal_title

Biochemistry

authors

Fedosov SN,Fedosova NU,Berglund L,Moestrup SK,Nexø E,Petersen TE

doi

10.1021/bi047936v

subject

Has Abstract

pub_date

2005-03-08 00:00:00

pages

3604-14

issue

9

eissn

0006-2960

issn

1520-4995

journal_volume

44

pub_type

杂志文章

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