Control of mucin synthesis: the peptide portion of synthetic O-glycopeptide substrates influences the activity of O-glycan core 1 UDPgalactose:N-acetyl-alpha-galactosaminyl-R beta 3-galactosyltransferase.

abstract：:An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...

journal_title：Biochemistry

authors： Vilsen B

更新日期：1993-12-07 00:00:00

abstract：:Helix-helix interactions within membranes are dominated by van der Waals packing motifs and side chain-side chain hydrogen bond formation, which act in tandem to determine the residues that comprise the interface between two given helices. To explore in a systematic manner the tertiary contacts between transmembrane h...

journal_title：Biochemistry

authors： Johnson RM,Heslop CL,Deber CM

更新日期：2004-11-16 00:00:00

abstract：:Calmodulin forms a covalent, one to one, complex with 3H-labeled norchlorpromazine isothiocyanate. Complex formation was monitored by high-performance liquid chromatography using a CN reverse-phase column which resolves calmodulin, the calmodulin-norchlorpromazine adduct, and norchlorpromazine isothiocyanate. Formatio...

journal_title：Biochemistry

authors： Newton DL,Burke TR Jr,Rice KC,Klee CB

更新日期：1983-11-22 00:00:00

abstract：:To test for on target toxicity of a new chemical entity, it is important to have comparable binding affinities of the compound in the target proteins from humans and the test species. To evaluate our myeloid cell leukemia-1 (Mcl-1) inhibitors, we tested them against rodent Mcl-1 and found a significant loss of binding...

journal_title：Biochemistry

authors： Zhao B,Arnold AL,Coronel MA,Lee JH,Lee T,Olejniczak ET,Fesik SW

更新日期：2018-08-14 00:00:00

abstract：:The thermal unfolding of rabbit skeletal heavy meromyosin (HMM), myosin subfragment 1, and subfragment 2 has been studied by differential scanning calorimetry (DSC). Two distinct endotherms are observed in the DSC scan of heavy meromyosin. The first endotherm, with a Tm of 41 degrees C at pH 7.9 in 0.1 M KCl, is assig...

journal_title：Biochemistry

authors： Shriver JW,Kamath U

更新日期：1990-03-13 00:00:00

abstract：:The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...

journal_title：Biochemistry

authors： Bures EJ,Hui JO,Young Y,Chow DT,Katta V,Rohde MF,Zeni L,Rosenfeld RD,Stark KL,Haniu M

更新日期：1998-09-01 00:00:00

abstract：:C4'-oxidized (C4-AP) and C5'-oxidized abasic sites (DOB) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross-links selectively with dA opposite a 3'-adjacent nucleotide, despite the comparable proximity of an opposing dA. A previous report on UvrABC incision of DNA su...

journal_title：Biochemistry

authors： Ghosh S,Greenberg MM

更新日期：2015-10-13 00:00:00

abstract：:Mixed phospholipid systems of ether-linked 1,2-dihexadecylphosphatidylcholine (DHPC) and ester-linked 1,2-dipalmitoylphosphatidylcholine (DPPC) have been studied by differential scanning calorimetry and X-ray diffraction. At maximum hydration (60 wt % water), DHPC shows three reversible transitions: a main (chain melt...

journal_title：Biochemistry

authors： Kim JT,Mattai J,Shipley GG

更新日期：1987-10-20 00:00:00

abstract：:The first epidermal growth factor-like domain (EGF-1) from blood coagulation factor VII (FVII) contains two unusual O-linked glycosylation sites at Ser-52 and Ser-60. We report here a detailed study of the effect of O-fucosylation at Ser-60 on the structure of FVII EGF-1, its Ca2+-binding affinity, and its interaction...

journal_title：Biochemistry

authors： Kao YH,Lee GF,Wang Y,Starovasnik MA,Kelley RF,Spellman MW,Lerner L

更新日期：1999-06-01 00:00:00

abstract：:Mutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two polypeptides. These residues have been proposed to determine the specificity of cohesin-doc...

journal_title：Biochemistry

authors： Schaeffer F,Matuschek M,Guglielmi G,Miras I,Alzari PM,Béguin P

更新日期：2002-02-19 00:00:00

abstract：:Steady-state kinetic parameters were determined for the human leukocyte elastase catalyzed hydrolysis of a series of peptide-based thiobenzyl esters and p-nitroanilides. The peptide units are MeOSuc-Val, MeOSuc-Alan-Pro-Val (n = 0-2), and MeOSuc-Alan-Pro-Ala (n = 1 or 2). The results of this study suggest five importa...

journal_title：Biochemistry

authors： Stein RL,Strimpler AM,Hori H,Powers JC

更新日期：1987-03-10 00:00:00

abstract：:Fluorescent nucleobase analogues are used extensively to probe the structure and dynamics of nucleic acids. The fluorescence of the adenine analogue 2-aminopurine and the cytosine analogue pyrrolocytosine is significantly quenched when the bases are located in regions of double-stranded nucleic acids. To allow more de...

journal_title：Biochemistry

authors： Hardman SJ,Thompson KC

更新日期：2006-08-01 00:00:00

abstract：:In cerebellum, inositol trisphosphate- (InsP(3)-) gated Ca channels play a key role in learning, though they exhibit a low sensitivity to InsP(3) compared to peripheral tissues. In the present study, the cerebellar InsP(3) receptor is shown to be associated with a novel inhibitor of InsP(3) binding. (3)H-InsP(3) bindi...

journal_title：Biochemistry

authors： Watras J,Orlando R,Moraru II

更新日期：2000-03-28 00:00:00

abstract：:The role of the "C-terminal tail" segment of long neurotoxins has been investigated. The C-terminal four to five residues of alpha-bungarotoxin and Laticauda colubrina b have been cleaved off by carboxypeptidase P. The effect of such deletion on the toxin conformation has been monitored in proton nuclear magnetic reso...

journal_title：Biochemistry

authors： Endo T,Oya M,Tamiya N,Hayashi K

更新日期：1987-07-14 00:00:00

abstract：:Tandem calponin-homology (CH) domains are the most common actin-binding domains in proteins. However, structural principles underlying their function are poorly understood. These tandem domains exist in multiple conformations with varying degrees of inter-CH-domain interactions. Dystrophin and utrophin tandem CH domai...

journal_title：Biochemistry

authors： Bandi S,Singh SM,Mallela KM

更新日期：2015-09-08 00:00:00

abstract：:The thermodynamic quantities of the anti-dinitrophenyl antibody-hapten interaction are reported for rabbit, goat, and guinea pig antibodies. Rabbit and goat antibodies had similar exothermic enthalpy changes for their reaction with 2,4-dinitrophenyl-L-lysine (-13.9 and -14.8 kcal/mol, respectively). The enthalpy chang...

journal_title：Biochemistry

authors： Halsey JF,Cebra JJ,Biltonen RL

更新日期：1975-11-18 00:00:00

abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...

journal_title：Biochemistry

authors： Smith SO,Palings I,Copié V,Raleigh DP,Courtin J,Pardoen JA,Lugtenburg J,Mathies RA,Griffin RG

更新日期：1987-03-24 00:00:00

abstract：:A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...

journal_title：Biochemistry

authors： Wu M,Arimura GK,Yunis AA

更新日期：1977-05-03 00:00:00

abstract：:The interaction between the apoprotein of 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans and the prosthetic group FAD has been investigated by 13C, 15N, and 31P NMR techniques. The FAD prosthetic group was selectively enriched in 13C and 15N isotopes by adding isotopically labeled riboflavin derivatives to the...

journal_title：Biochemistry

authors： Pust S,Vervoort J,Decker K,Bacher A,Müller F

更新日期：1989-01-24 00:00:00

abstract：:We find that specific oxidation for the Met-192 residue in delta-chymotrypsin to methionine sulfoxide results in a twofold increase in Km(app) and unchanged kcat in the hydrolysis of N-acetyl mono(amino acid) amide substrates. However, the catalyzed hydrolyses of N-acetyl dipeptide amide substrates by (methionine sulf...

journal_title：Biochemistry

authors： Treadway WJ Jr,Schultz RM

更新日期：1976-09-21 00:00:00

abstract：:Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...

journal_title：Biochemistry

authors： Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

更新日期：2013-01-15 00:00:00

abstract：:We have previously designed and synthesized small-molecule inhibitors that reduce Vibrio cholerae virulence in vitro by targeting the transcription factor ToxT. Here we report the synthesis and biological activity of derivatives of our previous bicyclic, fatty acid-like inhibitors. All of the synthesized derivatives s...

journal_title：Biochemistry

authors： Woodbrey AK,Onyango EO,Kovacikova G,Kull FJ,Gribble GW

更新日期：2018-09-25 00:00:00

abstract：:The cyclin-dependent kinase inhibitor p16(INK4a) (hereafter p16) functions as a multiple tumor suppressor. Mutations in p16, which are distributed throughout the entire protein, have been identified in a variety of human cancers and cancer-derived cell lines. It is unclear how tumor-derived mutations disrupt the struc...

journal_title：Biochemistry

authors： Zhang B,Peng ZY

更新日期：2002-05-21 00:00:00

abstract：:Eukaryotic transfer RNA precursors (pre-tRNAs) contain a 5' leader preceding the aminoacyl acceptor stem and a 3' trailer extending beyond this stem. An early step in pre-tRNA maturation is removal of the 5' leader by the endoribonuclease, RNase P. Extensive pairing between leader and trailer sequences has previously ...

journal_title：Biochemistry

authors： Ziehler WA,Day JJ,Fierke CA,Engelke DR

更新日期：2000-08-15 00:00:00

abstract：:Earlier work indicates that the major DNA repair phosphodiesterase (PDE) in yeast cells is the well-characterized Apn1 protein. Apn1 demonstrates both Mg2+-independent PDE activity and Mg2+-independent class II apurinic/apyrimidinic (AP) endonuclease activity and represents greater than 90% of the activity detected in...

journal_title：Biochemistry

authors： Sander M,Ramotar D

更新日期：1997-05-20 00:00:00

abstract：:To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...

journal_title：Biochemistry

authors： Qian W,Sun YL,Wang YH,Zhuang JH,Xie Y,Huang ZX

更新日期：1998-10-06 00:00:00

abstract：:The genomic and antigenomic RNAs of hepatitis delta virus are capable of self-cleavage and show no significant sequence similarities to other known self-cleaving RNAs. We have derived an antigenomic delta RNA which cleaves to completion in 15 s in 9 mM magnesium at 37 degrees C and is capable of efficient self-cleavag...

journal_title：Biochemistry

authors： Smith JB,Gottlieb PA,Dinter-Gottlieb G

更新日期：1992-10-13 00:00:00

abstract：:The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...

journal_title：Biochemistry

authors： Rahil J,Pratt RF

更新日期：1992-06-30 00:00:00

abstract：:There is no complete understanding of how serine protease inhibitors of the serpin family inhibit their target enzymes. Structural and biochemical studies have suggested that serpins utilize a mechanism that is distinct from the standard mechanism of inhibition proposed for most small protein protease inhibitors. Prot...

journal_title：Biochemistry

authors： Plotnick MI,Mayne L,Schechter NM,Rubin H

更新日期：1996-06-11 00:00:00

abstract：:The enzyme steroid 5 alpha-reductase utilizes NADPH to reduce the double bonds of a variety of steroid substrates with generalized 3-oxo, delta 4,5 structures. One substrate for this membrane-bound enzyme is testosterone, whose reduction to dihydrotestosterone is required for the embryonic differentiation of the exter...

journal_title：Biochemistry

authors： Wigley WC,Prihoda JS,Mowszowicz I,Mendonca BB,New MI,Wilson JD,Russell DW

更新日期：1994-02-08 00:00:00