Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells.

abstract：:Regulation of cellular response is an important mechanism for controlling cellular functions. The transmembrane signaling of the hormone receptors is regulated by GTP-binding proteins (GTPases) and their associated proteins. Our previous studies demonstrated that the bifunctional GTP-binding protein, G alpha h (transg...

journal_title：Biochemistry

authors： Baek KJ,Das T,Gray CD,Desai S,Hwang KC,Gacchui R,Ludwig M,Im MJ

更新日期：1996-02-27 00:00:00

abstract：:Dog serum and colostral immunoglobulin A (IgA) and free secretory component from colostrum were isolated using affinity chromatography. Both serum and colostral IgA showed similar susceptibility to reduction with dithiothreitol, but only colostral IgA released the additional subunit, bound secretory component. This re...

journal_title：Biochemistry

authors： Thompson RE,Reynolds HY,Waxdal MJ

更新日期：1975-07-01 00:00:00

abstract：:The structural protein nonerythroid alpha spectrin (alphaIISp) plays a role in the repair of DNA interstrand cross-links and is deficient in cells from patients with Fanconi anemia (FA), in which there is a defect in ability to repair such cross-links. We have proposed a model in which alphaIISp, whose stability is de...

journal_title：Biochemistry

authors： Lefferts JA,Wang C,Sridharan D,Baralt M,Lambert MW

更新日期：2009-01-20 00:00:00

abstract：:Electrogenic events associated with the activity of the melibiose permease (MelB), a transporter from Escherichia coli, were investigated. Proteoliposomes containing purified MelB were adsorbed to a solid supported lipid membrane, activated by a substrate concentration jump, and transient currents were measured. When ...

journal_title：Biochemistry

authors： Meyer-Lipp K,Ganea C,Pourcher T,Leblanc G,Fendler K

更新日期：2004-10-05 00:00:00

abstract：:Substitution by site-directed mutagenesis of any one of the histidine residues H721, H837, and H852 by glutamine, or of H752 by serine, inhibits Cl- flux mediated by band 3 expressed in Xenopus oocytes. Mutation of Lys 558 (K558N), the site of covalent binding of H2DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonate) i...

journal_title：Biochemistry

authors： Müller-Berger S,Karbach D,König J,Lepke S,Wood PG,Appelhans H,Passow H

更新日期：1995-07-25 00:00:00

abstract：:The labeling of chloroplast coupling factor 1 by 3'-O-(4-benzoyl)benzoyl-ATP (BzATP) was studied. When the enzyme was incubated with approximately 10 microM BzATP and 6 mM MgCl2 at pH 7.9 for approximately 20 min and passed through two Sephadex G-50 centrifuge columns, three BzATP molecules were bound per coupling fac...

journal_title：Biochemistry

authors： Admon A,Hammes GG

更新日期：1987-06-02 00:00:00

abstract：:The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding sites and that experimentally applied ANS concentration...

journal_title：Biochemistry

authors： Saucier AC,Mariotti S,Anderson SA,Purich DL

更新日期：1985-12-17 00:00:00

abstract：:Three crystal structures have been determined of active site specific substituted Cd(II) horse liver alcohol dehydrogenase and its complexes. Intensities were collected for the free, orthorhombic enzyme to 2.4-A resolution and for a triclinic binary complex with NADH to 2.7-A resolution. A ternary complex was crystall...

journal_title：Biochemistry

authors： Schneider G,Cedergren-Zeppezauer E,Knight S,Eklund H,Zeppezauer M

更新日期：1985-12-03 00:00:00

abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...

journal_title：Biochemistry

authors： Green KD,Biswas T,Pang AH,Willby MJ,Reed MS,Stuchlik O,Pohl J,Posey JE,Tsodikov OV,Garneau-Tsodikova S

更新日期：2018-02-06 00:00:00

abstract：:Insulin decreases multifunctional protein kinase (MFPK) activity in rat adipose tissue [Ramakrishna, S., & Benjamin, W. B. (1988) J. Biol. Chem. 263, 12677-12681]. Insulin also decreases the phosphorylation of peptide B but increases the phosphorylation of peptide A of ATP-citrate lyase (ATP-CL). The mechanism for thi...

journal_title：Biochemistry

authors： Ramakrishna S,Murthy KS,Benjamin WB

更新日期：1989-01-24 00:00:00

abstract：:Four mechanisms for the allosteric regulation of the calcium and magnesium ion activated adenosinetriphosphatase (Ca,Mg-ATPase) of sarcoplasmic reticulum were examined. Negative cooperativity in substrate binding was not supported by 3H-labeled 5'-adenylyl methylenediphosphate (AMPPCP) binding, which was best fit by a...

journal_title：Biochemistry

authors： Cable MB,Feher JJ,Briggs FN

更新日期：1985-09-24 00:00:00

abstract：:Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this re...

journal_title：Biochemistry

authors： Omoto CK,Johnson KA

更新日期：1986-01-28 00:00:00

abstract：:Dynamin 1 is thought to mediate synaptic transmission through interactions with multiple endocytic accessory proteins in a phosphorylation-dependent manner. Previously, we have shown that DYRK1A, a chromosome 21-encoded kinase implicated in the mental retardation of Down syndrome, phosphorylates primarily serine 857 (...

journal_title：Biochemistry

authors： Xie W,Adayev T,Zhu H,Wegiel J,Wieraszko A,Hwang YW

更新日期：2012-08-28 00:00:00

abstract：:A new class of bacterial multisubunit membrane-bound electron-transfer complexes has been identified based on biochemical and bioinformatic data. It contains subunits homologous to the three-subunit molybdopterin oxidoreductases and four additional subunits, two of which are c-type cytochromes. The complex was purifie...

journal_title：Biochemistry

authors： Yanyushin MF,del Rosario MC,Brune DC,Blankenship RE

更新日期：2005-08-02 00:00:00

abstract：:Beta-2-microglobulin (beta2m) deposits as amyloid fibrils in the musculoskeletal system of patients undergoing long-term dialysis treatment as a result of kidney failure. Previous work has shown that Cu(II) binding causes beta2m to organize into nativelike dimers and tetramers that precede amyloid formation. Cu(II) is...

journal_title：Biochemistry

authors： Srikanth R,Mendoza VL,Bridgewater JD,Zhang G,Vachet RW

更新日期：2009-10-20 00:00:00

abstract：:The intermediates in the oxidation of deoxyhemoglobin by ferricyanide in 0.1 M KCl, at 20 degrees C and three pH values, were studied by cryogenic techniques. Data analysis was carried out according to a simple four rate constant model, ignoring the functional heterogeneity of the subunits, to simulate the time course...

journal_title：Biochemistry

authors： Perrella M,Shrager RI,Ripamonti M,Manfredi G,Berger RL,Rossi-Bernardi L

更新日期：1993-05-18 00:00:00

abstract：:Phosphotriesterase, as isolated from Pseudomonas diminuta, is capable of detoxifying widely used pesticides such as paraoxon and parathion and various mammalian acetylcholinesterase inhibitors. The enzyme requires a binuclear metal center for activity. Recently, the three-dimensional structure of the apoenzyme was sol...

journal_title：Biochemistry

authors： Benning MM,Kuo JM,Raushel FM,Holden HM

更新日期：1995-06-27 00:00:00

abstract：:Horse heart cytochrome c has been modified by 8-azido-ATP and the electron-transfer activity of the modified cytochrome c's to bovine heart cytochrome c oxidase (CcO) under physiological ionic strengths has been studied by the laser flash photolysis technique with 5-deazariboflavin and EDTA as the electron donor. The ...

journal_title：Biochemistry

authors： Lin J,Wu S,Lau WT,Chan SI

更新日期：1995-02-28 00:00:00

abstract：:Two intermediates in the biosynthetic pathway to bacterial menaquinones are o-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid. Cell-free extracts have been prepared from Escherichia coli which catalyze the conversion of labeled o-succinylbenzoic acid to the naphthoic acid and also to menaquinones. The naphthoa...

journal_title：Biochemistry

authors： Bryant RW Jr,Bentley R

更新日期：1976-11-02 00:00:00

abstract：:Naturally occurring interruptions in nonfibrillar collagen play key roles in molecular flexibility, collagen degradation, and ligand binding. The structural feature of the interruption sequences and the molecular basis for their functions have not been well studied. Here, we focused on a G5G type natural interruption ...

journal_title：Biochemistry

authors： Xu T,Zhou CZ,Xiao J,Liu J

更新日期：2018-02-20 00:00:00

abstract：:Purification and potential tachykinin and enkephalin precursor cleaving enzymes from bovine chromaffin granules was undertaken using as substrates the model precursors 35S-(Met)-beta-preprotachykinin [35S-(Met)-beta-PPT] and 35S-(Met)-preproenkephalin [35S-(Met)-PPE]. Purification by concanavalin A-Sepharose, Sephacry...

journal_title：Biochemistry

authors： Krieger TJ,Hook VY

更新日期：1992-05-05 00:00:00

abstract：:Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of the fragile X mental retardation protein (FMRP). The emerging picture is that FMRP is involved in repression of translation through a complex network of protein-protein and protein-RNA interactions. Very little struc...

journal_title：Biochemistry

authors： Adinolfi S,Ramos A,Martin SR,Dal Piaz F,Pucci P,Bardoni B,Mandel JL,Pastore A

更新日期：2003-09-09 00:00:00

abstract：:The accessibility of the retinal Schiff base in bacteriorhodopsin was studied in the D85N/D96N mutant where the proton acceptor and donor are absent. Protonation and deprotonation of the Schiff base after pH jump without illumination and in the photocycle of the unprotonated Schiff base were measured in the visible an...

journal_title：Biochemistry

authors： Brown LS,Dioumaev AK,Needleman R,Lanyi JK

更新日期：1998-03-17 00:00:00

abstract：:New classes of small proteins have recently been found that refold rapidly with two-state kinetics from a substantially unfolded conformation ("U") and without the accumulation of a folding intermediate. Barnase, on the other hand, is representative of a class of proteins that display multistate kinetics and refold fr...

journal_title：Biochemistry

authors： Oliveberg M,Fersht AR

更新日期：1996-02-27 00:00:00

abstract：:Through a ligand-directed tosyl (LDT) chemistry strategy using the synthetic acetogenin ligand AL1, we succeeded in the pinpoint alkynylation (-C≡CH) of Asp160 in the 49 kDa subunit of bovine complex I, which may be located in the inner part of the putative quinone binding cavity of the enzyme [Masuya, T., et al. (201...

journal_title：Biochemistry

authors： Masuya T,Murai M,Morisaka H,Miyoshi H

更新日期：2014-12-16 00:00:00

abstract：:Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...

journal_title：Biochemistry

authors： Buchko GW,Tarasevich BJ,Bekhazi J,Snead ML,Shaw WJ

更新日期：2008-12-16 00:00:00

abstract：:Fourier transform infrared spectra of light-adapted bacteriorhodopsin exhibit a band at 1618 cm(-1) that shifts to 1625 cm(-1) upon formation of the L intermediate. It is assigned to the peptide C==O of Val49 from the fact that it shifts in [1-(13)C]valine-labeled bacteriorhodopsin and appears perturbed in the Val49--...

journal_title：Biochemistry

authors： Yamazaki Y,Tuzi S,Saitô H,Kandori H,Needleman R,Lanyi JK,Maeda A

更新日期：1996-04-02 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with...

journal_title：Biochemistry

authors： Lofstad M,Gudim I,Hammerstad M,Røhr ÅK,Hersleth HP

更新日期：2016-09-13 00:00:00

abstract：:The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...

journal_title：Biochemistry

authors： Kautiainen TL,Jones PA

更新日期：1985-02-26 00:00:00