Purification and characterization of a cathepsin D protease from bovine chromaffin granules.

abstract：:Steroid receptors define a family of ligand-activated transcription factors. Recent work has demonstrated that the receptors regulate distinct but overlapping gene networks, yet the mechanisms by which they do so remain unclear. We previously determined the microscopic binding energetics for progesterone receptor (PR)...

journal_title：Biochemistry

authors： Moody AD,Miura MT,Connaghan KD,Bain DL

更新日期：2012-01-24 00:00:00

abstract：:The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...

journal_title：Biochemistry

authors： Lipman RS,Jorns MS

更新日期：1996-06-18 00:00:00

abstract：:Pulsed electron paramagnetic resonance spectroscopy has been used to detect Mn(II)-ligand superhyperfine couplings in complexes with creatine kinase and in the Mn(II) metalloprotein concanavalin A. Electron spin-echo envelopes from Mn(II), bound in these complexes, are modulated by superhyperfine interactions between ...

journal_title：Biochemistry

authors： LoBrutto R,Smithers GW,Reed GH,Orme-Johnson WH,Tan SL,Leigh JS Jr

更新日期：1986-09-23 00:00:00

abstract：:A ribonuclease that hydrolyzes either linear duplex or single-stranded RNA in an exonucleolytic manner has been partially purified from Ehrlich ascites tumor cell nucleoli and is free from other ribonucleases. The enzyme will also degrade the RNA complement of an RNA X DNA duplex; however, no nuclease activity is obse...

journal_title：Biochemistry

authors： Eichler DC,Eales SJ

更新日期：1985-01-29 00:00:00

abstract：:The oxidation of deoxy- and oxyhemerythrin to methemerythrin by Fe(CN)6(3-) has been examined. The first step produces (semi-met)O, a half-oxidized form of hemerythrin which has different spectral and kinetic properties from (semi-met)R which is produced by one-electron reduction of methemerythrin. Further oxidation o...

journal_title：Biochemistry

authors： Bradić Z,Harrington PC,Wilkins RG,Yoneda G

更新日期：1980-09-02 00:00:00

abstract：:Fourier transform infrared difference spectroscopy has been used to obtain information about substrate-induced structural changes of the melibiose permease (MelB) from Escherichia coli reconstituted into liposomes. Binding of the cosubstrate Na(+) gives rise to several peaks in the amide I and II regions of the differ...

journal_title：Biochemistry

authors： León X,Lórenz-Fonfría VA,Lemonnier R,Leblanc G,Padrós E

更新日期：2005-03-08 00:00:00

abstract：:The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...

journal_title：Biochemistry

authors： Agarwal GP,Gallagher JG,Aune KC,Armeniades CD

更新日期：1977-05-03 00:00:00

abstract：:Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...

journal_title：Biochemistry

authors： Wu F,Flach CR,Seaton BA,Mealy TR,Mendelsohn R

更新日期：1999-01-12 00:00:00

abstract：:Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...

journal_title：Biochemistry

authors： Bennett N,Clerc A

更新日期：1992-02-18 00:00:00

abstract：:Plexins are transmembrane proteins that serve as guidance receptors during angiogenesis, lymphangiogenesis, neuronal development, and zebrafish fin regeneration, with a putative role in cancer metastasis. Receptor dimerization or clustering, induced by extracellular ligand binding but modulated in part by the plexin t...

journal_title：Biochemistry

authors： Barton R,Khakbaz P,Bera I,Klauda JB,Iovine MK,Berger BW

更新日期：2016-09-06 00:00:00

abstract：:Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...

journal_title：Biochemistry

authors： Dolence JM,Steward LE,Dolence EK,Wong DH,Poulter CD

更新日期：2000-04-11 00:00:00

abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...

journal_title：Biochemistry

authors： Thomas F,Niitsu A,Oregioni A,Bartlett GJ,Woolfson DN

更新日期：2017-12-19 00:00:00

abstract：:Human immunoglobulin E (IgE) consists of 14 domains, each with the characteristic immunoglobulin fold structure. Compared with the 12-domain structure of immunoglobulin G (IgG), IgE has an additional pair of domains (C epsilon 2) in the Fc region in place of the hinge of IgG. The crystal structure of the 4-domain Fc f...

journal_title：Biochemistry

authors： Beavil AJ,Young RJ,Sutton BJ,Perkins SJ

更新日期：1995-11-07 00:00:00

abstract：:DNA alkylation-induced damage is one of the most efficacious anticancer therapeutic strategies. Enhanced DNA alkylation and weakened DNA repair capacity in cancer cells are responsible for the effectiveness of DNA-alkylating therapies. 5'-Flap endonuclease 1 (Fen1) is an important enzyme involved in base excision repa...

journal_title：Biochemistry

authors： Panda H,Jaiswal AS,Corsino PE,Armas ML,Law BK,Narayan S

更新日期：2009-10-27 00:00:00

abstract：:Aquaporin-4 (AQP4) is a water channel found at high concentrations around blood vessels in the brain and is organized into elaborate assemblies called square arrays. The natural functions of AQP4 and the square arrays remain unknown, but under pathophysiological conditions, AQP4 has been shown to influence brain edema...

journal_title：Biochemistry

authors： Strand L,Moe SE,Solbu TT,Vaadal M,Holen T

更新日期：2009-06-30 00:00:00

abstract：:Calcium is required for the substrate binding and for the chemical step of the interfacial catalytic turnover cycle of pancreatic phospholipase A2 (PLA2), but not for the binding of the enzyme to the interface. The role of calcium and other divalent cations (C) is analyzed for the effect on the substrate binding and k...

journal_title：Biochemistry

authors： Yu BZ,Rogers J,Nicol GR,Theopold KH,Seshadri K,Vishweshwara S,Jain MK

更新日期：1998-09-08 00:00:00

abstract：:F62 LOS of Neisseria gonorrhoeae consists of two major LOS components; the higher and smaller molecular weight (MW) components were recognized by MAbs 1-1-M and 3F11 respectively. Base-line separation of the two major oligosaccharide (OS) components from F62 LOS was achieved by Bio-Gel P-4 chromatography after dephosp...

journal_title：Biochemistry

authors： Yamasaki R,Bacon BE,Nasholds W,Schneider H,Griffiss JM

更新日期：1991-10-29 00:00:00

abstract：:The inactivation of excited rhodopsin in the presence of ATP, rhodopsin kinase, and/or arrestin has been studied from its effect on the two subsequent steps in the light-induced enzymatic cascade: metarhodopsin II catalyzed activation of G-protein and G-protein-dependent activation of cGMP phosphodiesterase. The inact...

journal_title：Biochemistry

authors： Bennett N,Sitaramayya A

更新日期：1988-03-08 00:00:00

abstract：:Addition of short sequences of dCMP residues to the 3'-OH end of duplex linear DNAs allows rapid and efficient transcription to be initiated at these sites by purified mammalian RNA polymerase II [Kadesch, T. R., & Chamberlin, M. J. (1982) J. Biol. Chem. 257, 5286-5295]. The use of such tailed DNA templates should all...

journal_title：Biochemistry

authors： Dedrick RL,Chamberlin MJ

更新日期：1985-04-23 00:00:00

abstract：:The periplasmic protein CusF, as a part of the CusCFBA efflux complex, plays a role in resistance to elevated levels of copper and silver in Escherichia coli. Although homologues have been identified in other Gram-negative bacteria, the substrate of CusF and its precise role in metal resistance have not been described...

journal_title：Biochemistry

authors： Kittleson JT,Loftin IR,Hausrath AC,Engelhardt KP,Rensing C,McEvoy MM

更新日期：2006-09-19 00:00:00

abstract：:(R)-N(delta)-(N'-Sulfodiaminophosphinyl)-L-ornithine (PSorn) is the active component of a phytotoxin, called phaseolotoxin, produced by Pseudomonas savastanoi pv. phaseolicola. PSorn acts as a potent transition state (TS) inhibitor of ornithine transcarbamoylase (OTCase, E.C. 2.1.3.3) that binds to the OTCase from Esc...

journal_title：Biochemistry

authors： Templeton MD,Reinhardt LA,Collyer CA,Mitchell RE,Cleland WW

更新日期：2005-03-22 00:00:00

abstract：:We have performed microsecond (μs) molecular dynamics simulation (MDS) to identify structural mechanisms for sarcolipin (SLN) uncoupling of Ca2+ transport from ATP hydrolysis for the sarcoplasmic reticulum Ca2+-ATPase (SERCA). SLN regulates muscle metabolism and energy expenditure to provide resistance against diet-in...

journal_title：Biochemistry

authors： Autry JM,Thomas DD,Espinoza-Fonseca LM

更新日期：2016-11-08 00:00:00

abstract：:The environmentally sensitive fluorescent probes 6-propionyl-2-(N,N-dimethylamino)naphthalene (PRODAN) and 2'-(N,N-dimethylamino)-6-naphthoyl-4-trans-cyclohexanioc acid (DANCA) form complexes with the heme binding site of apohorseradish peroxidase. The dissociation constants of the PRODAN and DANCA complexes were dete...

journal_title：Biochemistry

authors： Lasagna M,Vargas V,Jameson DM,Brunet JE

更新日期：1996-01-23 00:00:00

abstract：:The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...

journal_title：Biochemistry

authors： Wang QW,Kline AD,Wüthrich K

更新日期：1987-10-06 00:00:00

abstract：:Defects in the MMACHC gene represent the most common disorder of cobalamin (Cbl) metabolism, affecting synthesis of the enzyme cofactors adenosyl-Cbl and methyl-Cbl. The encoded MMACHC protein binds intracellular Cbl derivatives with different upper axial ligands and exhibits flavin mononucleotide (FMN)-dependent decy...

journal_title：Biochemistry

authors： Froese DS,Krojer T,Wu X,Shrestha R,Kiyani W,von Delft F,Gravel RA,Oppermann U,Yue WW

更新日期：2012-06-26 00:00:00

abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...

journal_title：Biochemistry

authors： Longstaff C,Gaffney PJ

更新日期：1991-01-29 00:00:00

abstract：:Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris is a highly specific heme-containing enzyme from a small family of homologous enzymes, which includes indoleamine 2,3-dioxygenase (IDO). The structure of wild type (WT TDO) in the catalytically active, ferrous (Fe (2+)) form and in complex with its substrate...

journal_title：Biochemistry

authors： Thackray SJ,Bruckmann C,Anderson JL,Campbell LP,Xiao R,Zhao L,Mowat CG,Forouhar F,Tong L,Chapman SK

更新日期：2008-10-07 00:00:00

abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...

journal_title：Biochemistry

authors： Wu HY,Behe MJ

更新日期：1985-09-24 00:00:00

abstract：:The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on...

journal_title：Biochemistry

authors： Labuda D,Pörschke D

更新日期：1980-08-05 00:00:00

abstract：:A procedure for universal 13C and/or 15N labeling of microbial peptides which are produced by fermentation in complex media and its application to two food-preserving bacteriocins from lactic acid bacteria are described. Isotopic enrichment of nisin A (from Lactococcus lactis) and of leucocin A (from Leuconostoc gelid...

journal_title：Biochemistry

authors： Sailer M,Helms GL,Henkel T,Niemczura WP,Stiles ME,Vederas JC

更新日期：1993-01-12 00:00:00