Purification and properties of a novel nucleolar exoribonuclease capable of degrading both single-stranded and double-stranded RNA.

abstract：:Two oxygenated iron-cobalt hybrid hemoglobins (Hbs), (alpha Co-O2 beta Fe-O2)2 and (alpha Fe-O2 beta Co-O2)2, were studied by electron spin echo envelope modulation (ESEEM) spectroscopy in order to measure (i) electron-nuclear hyperfine and nuclear quadrupole coupling to the N epsilon of the proximal histidyl imidazol...

journal_title：Biochemistry

authors： Lee HC,Peisach J,Tsuneshige A,Yonetani T

更新日期：1995-05-23 00:00:00

abstract：:Legumain (asparaginyl endopeptidase) is the only protease with a preference for cleavage after asparagine residues. Increased legumain activity is a hallmark of inflammation, neurodegenerative diseases, and cancer, and legumain inhibitors have exhibited therapeutic effects in mouse models of these pathologies. Improve...

journal_title：Biochemistry

authors： Anderson BM,de Almeida LGN,Sekhon H,Young D,Dufour A,Edgington-Mitchell LE

更新日期：2020-01-28 00:00:00

abstract：:Vitamin D receptor (VDR) is a member of the nuclear hormone receptor superfamily. When bound to a variety of vitamin D analogues, VDR manifests a wide diversity of physiological actions. The molecular mechanism by which different vitamin D analogues cause specific responses is not understood. The published crystallogr...

journal_title：Biochemistry

authors： Singarapu KK,Zhu J,Tonelli M,Rao H,Assadi-Porter FM,Westler WM,DeLuca HF,Markley JL

更新日期：2011-12-27 00:00:00

abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

journal_title：Biochemistry

authors： Weidman SW,Drysdale GR

更新日期：1979-08-21 00:00:00

abstract：:The temporal evolution of the near-infrared stimulated emission band of the special pair excited state (P*) in the reaction center of Rhodobacter sphaeroides has been studied in intracytoplasmic membranes of the antenna-deficient RCO1 mutant at 10 K with a resolution of 30 fs. On the 100-fs time scale the emission ban...

journal_title：Biochemistry

authors： Vos MH,Jones MR,Hunter CN,Breton J,Lambry JC,Martin JL

更新日期：1994-06-07 00:00:00

abstract：:The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...

journal_title：Biochemistry

authors： Drohat AC,Xiao G,Tordova M,Jagadeesh J,Pankiewicz KW,Watanabe KA,Gilliland GL,Stivers JT

更新日期：1999-09-14 00:00:00

abstract：:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...

journal_title：Biochemistry

authors： Casella L,Poli S,Gullotti M,Selvaggini C,Beringhelli T,Marchesini A

更新日期：1994-05-31 00:00:00

abstract：:Phosphorylation of the sarcoplasmic reticulum calcium ATPase, E, is first order with kb = 70 +/- 7 s-1 after free enzyme was mixed with saturating ATP and 50 microM Ca2+; this is one-third the rate constant of 220 s-1 for phosphorylation of enzyme preincubated with calcium, cE.Ca2, after being mixed with ATP under the...

journal_title：Biochemistry

authors： Stahl N,Jencks WP

更新日期：1987-12-01 00:00:00

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family counteract protein misfolding in a variety of neurodegenerative disease models. To determine whether human Hsp70 exerts similar effects on the aggregation of alpha-synuclein (alpha-Syn), the key component of insoluble fibrils present in Parkinson's disea...

journal_title：Biochemistry

authors： Luk KC,Mills IP,Trojanowski JQ,Lee VM

更新日期：2008-11-25 00:00:00

abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

journal_title：Biochemistry

authors： Kuhlmann J,Macara I,Wittinghofer A

更新日期：1997-10-07 00:00:00

abstract：:Triple-helical DNA shows increasing potential for applications in the control of gene expression (including therapeutics) and the development of sequence-specific DNA-cleaving agents. The major limitation in this technology has been the requirement of homopurine sequences for triplex formation. We describe a simple ap...

journal_title：Biochemistry

authors： Jayasena SD,Johnston BH

更新日期：1992-01-21 00:00:00

abstract：:Cardiac troponin T (cTnT) is a myofibrillar protein essential for calcium-dependent contraction. This property has led to functional studies of developmentally expressed cTnT isoforms and mutants identified in patients with hypertrophic cardiomyopathy. The release of cTnT into the serum following myocardial infarction...

journal_title：Biochemistry

authors： Lounes KC,Demeler B,Anderson DE,Gomes AW,Potter JD,Nassar R,Anderson PA

更新日期：2008-02-19 00:00:00

abstract：:Solution scattering studies were performed on a ligand-binding domain (S1S2) of a glutamate receptor ion channel (GluR) in order to study GluR-binding and signal-transduction mechanisms. The core of the ligand-binding domain is homologous to prokaryotic periplasmic binding proteins (PBP), whose binding mechanism invol...

journal_title：Biochemistry

authors： Abele R,Svergun D,Keinänen K,Koch MH,Madden DR

更新日期：1999-08-24 00:00:00

abstract：:Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...

journal_title：Biochemistry

authors： Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

更新日期：1999-03-02 00:00:00

abstract：:The product of yjeK in Escherichia coli is a homologue of lysine 2,3-aminomutase (LAM) from Clostridium subterminale SB4, and both enzymes catalyze the isomerization of (S)- but not (R)-alpha-lysine by radical mechanisms. The turnover number for LAM from E. coli is 5.0 min(-1), 0.1% of the value for clostridial LAM. T...

journal_title：Biochemistry

authors： Behshad E,Ruzicka FJ,Mansoorabadi SO,Chen D,Reed GH,Frey PA

更新日期：2006-10-24 00:00:00

abstract：:The neonatal Fc receptor (FcRn) binds maternal immunoglobulin G (IgG) during the acquisition of passive immunity by the fetus or newborn. In adult mammals, FcRn also binds IgG and returns it to the bloodstream, thus protecting IgG from a default degradative pathway. Biosensor assays have been used to characterize the ...

journal_title：Biochemistry

authors： Vaughn DE,Bjorkman PJ

更新日期：1997-08-05 00:00:00

abstract：:The fluorescence quenching of 9-aminoacridine (9AA) in suspension of beef heart EDTA submitochondrial particles was studied and was used to calculate the pH gradient between these particles and the medium. This pH gradient, which is energy dependent, is also dependent strongly on the presence of anion species in the m...

journal_title：Biochemistry

authors： Rottenberg H,Lee CP

更新日期：1975-06-17 00:00:00

abstract：:Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...

journal_title：Biochemistry

authors： McParland V,Varsano G,Li X,Thornton J,Baby J,Aravind A,Meyer C,Pavic K,Rios P,Köhn M

更新日期：2011-09-06 00:00:00

abstract：:Recently, it was demonstrated that bifunctional catalase-peroxidases (KatGs) are found not only in archaea and bacteria but also in lower eukaryotes. Structural studies and preliminary biochemical data of the secreted KatG from the rice pathogen Magnaporthe grisea (MagKatG2) suggested both similar and novel features w...

journal_title：Biochemistry

authors： Gasselhuber B,Carpena X,Graf MM,Pirker KF,Nicolussi A,Sündermann A,Hofbauer S,Zamocky M,Furtmüller PG,Jakopitsch C,Oostenbrink C,Fita I,Obinger C

更新日期：2015-09-08 00:00:00

abstract：:The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...

journal_title：Biochemistry

authors： Jucker FM,Phillips RM,McCallum SA,Pardi A

更新日期：2003-03-11 00:00:00

abstract：:Fourteen oligonucleotides 8-21 nucleotides in length and their complements were synthesized as DNA and RNA. For each sequence, four kinds of duplexes, DNA:DNA, RNA:RNA, DNA:RNA, and RNA:DNA, were prepared. Twelve sequences had A.T/U content varying from 25 to 80% and dPy content in the DNA strands varying from 0 to 10...

journal_title：Biochemistry

authors： Lesnik EA,Freier SM

更新日期：1995-08-29 00:00:00

abstract：:The binding of human Glu- and Lys-plasminogens to intact fibrin clots, to lysine-Sepharose, and to fibrin cleaved by plasmin was quantitatively characterized. On intact fibrin clots, there was one strong binding site for Glu-plasminogen with a dissociation constant, Kd, of 25 microM and one strong binding site for Lys...

journal_title：Biochemistry

authors： Bok RA,Mangel WF

更新日期：1985-06-18 00:00:00

abstract：:Two paramagnetic probes, viz., Mn2+ and Cr3+-ATP, were used to map distances to various loci on carbamoyl-phosphate synthetase by using NMR measurements. The paramagnetic influence of Mn2+ on the 1H of L-glutamate and L-ornithine was measured at 200 and 360 MHz. On the basis of these data, a correlation time for the p...

journal_title：Biochemistry

authors： Raushel FM,Anderson PM,Villafranca JJ

更新日期：1983-04-12 00:00:00

abstract：:Endoglucanase Cel6A from Thermobifida fusca hydrolyzes the beta-1,4 linkages in cellulose at accessible points along the polymer. The structure of the catalytic domain of Cel6A from T. fusca in complex with a nonhydrolysable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside (Glc(...

journal_title：Biochemistry

authors： Larsson AM,Bergfors T,Dultz E,Irwin DC,Roos A,Driguez H,Wilson DB,Jones TA

更新日期：2005-10-04 00:00:00

abstract：:The distribution of one-disulfide bonds in the two-disulfide intermediates in the oxidative refolding of bovine pancreatic ribonuclease A has been characterized. These two-disulfide intermediates were formed from the fully reduced denatured protein by oxidation with dithiothreitol, then blocked with AEMTS, purified by...

journal_title：Biochemistry

authors： Volles MJ,Xu X,Scheraga HA

更新日期：1999-06-01 00:00:00

abstract：:N-Terminal glutaminyl and glutamyl residues of peptides and proteins tend to form pyroglutamic acid (pGlu) by intramolecular cylization. The rate constants for spontaneous cyclization of glutamine (10(-6) s(-1)) and glutamic acid (10(-9) s(-1)) in aqueous solution differ by approximately 3 orders of magnitude at pH 6....

journal_title：Biochemistry

authors： Seifert F,Schulz K,Koch B,Manhart S,Demuth HU,Schilling S

更新日期：2009-12-22 00:00:00

abstract：:The cyanogen bromide fragment comprising residues 115-181 of Kunitz soybean trypsin inhibitor is a soluble random-coil peptide at pH 7 containing two cysteines separated by eight other amino acids in the primary sequence. Four of the six rate constants have been determined for the three disulfide exchange reactions be...

journal_title：Biochemistry

authors： Snyder GH

更新日期：1987-02-10 00:00:00

abstract：:There is much debate whether the fatty acid substrate of lipoxygenase binds "carboxylate-end first" or "methyl-end first" in the active site of soybean lipoxygenase-1 (sLO-1). To address this issue, we investigated the sLO-1 mutants Trp500Leu, Trp500Phe, Lys260Leu, and Arg707Leu with steady-state and stopped-flow kine...

journal_title：Biochemistry

authors： Ruddat VC,Mogul R,Chorny I,Chen C,Perrin N,Whitman S,Kenyon V,Jacobson MP,Bernasconi CF,Holman TR

更新日期：2004-10-19 00:00:00

abstract：:tRNase Z, which exists in almost all cells, is believed to be working primarily for tRNA 3' maturation. In Escherichia coli, however, the tRNase Z gene appears to be dispensable under normal growth conditions, and its physiological role is not clear. Here, to investigate a possibility that E. coli tRNase Z cleaves RNA...

journal_title：Biochemistry

authors： Shibata HS,Minagawa A,Takaku H,Takagi M,Nashimoto M

更新日期：2006-05-02 00:00:00

abstract：:The present study was carried out to determine the energetics of Clostridium symbiosum pyruvate phosphate dikinase (PPDK) catalyzed interconversion of adenosine 5'-triphosphate (ATP), orthophosphate (Pi), and pyruvate (pyr) with adenosine 5'-monophosphate (AMP), inorganic pyrophosphate, and phosphoenolpyruvate (PEP) [...

journal_title：Biochemistry

authors： Mehl A,Xu Y,Dunaway-Mariano D

更新日期：1994-02-08 00:00:00