Interactions between Hsp70 and the hydrophobic core of alpha-synuclein inhibit fibril assembly.

abstract：:Seventeen ribosomal proteins of Saccharomyces cerevisiae were isolated from small ribosomal subunits and disodium ethylenediaminetetraacetate treated 80S ribosomes by chromatography on a column of carboxymethylcellulose and/or by filtration through Sephacryl S-200. The isolated proteins are YS4, YS7, YS8, YS9, YS10, Y...

journal_title：Biochemistry

authors： Otaka E,Higo K,Osawa S

更新日期：1982-09-14 00:00:00

abstract：:The electric organ of the ray, Torpedo ocellata, can serve as a source for both the acetylcholine (ACh) receptor and its ionic channel. The two entities were identified by their specific binding of [3H]ACh and [3H]perhydrohistrionicotoxin ([3H]H12-HTX), respectively. Binding of [3H]H12-HTX was inhibited by certain dru...

journal_title：Biochemistry

authors： Eldefrawi ME,Eldefrawi AT,Mansour NA,Daly JW,Witkop B,Albuquerque EX

更新日期：1978-12-12 00:00:00

abstract：:We constructed and expressed chimeric receptor cDNAs with insulin receptor exon 3 (residues 191-297 of the cysteine-rich region) replaced with either the comparable region of the insulin-like growth factor I receptor (IGF-IR) or the insulin receptor related receptor (IRR). Both chimeric receptors still could bind insu...

journal_title：Biochemistry

authors： Zhang B,Roth RA

更新日期：1991-05-28 00:00:00

abstract：:The structure of the 56-residue B1 immunoglobulin-binding domain from streptococcal protein G has been determined in two different crystal forms. The crystal structures were deduced by molecular replacement, based on the structure of the B2 domain (Brookhaven accession code 1PGX). Final R values are 0.174 and 0.198 fo...

journal_title：Biochemistry

authors： Gallagher T,Alexander P,Bryan P,Gilliland GL

更新日期：1994-04-19 00:00:00

abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...

journal_title：Biochemistry

authors： Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

更新日期：1999-06-22 00:00:00

abstract：:Mutation of Gly90, Glu113, Ala292, and Lys296 in the visual pigment rhodopsin constitutively activates the protein for activation of the G protein transducin. Three of these mutations have been shown to cause two different human diseases. Mutation of Gly90 and Ala292 results in complete night blindness, and mutation o...

journal_title：Biochemistry

authors： Rim J,Oprian DD

更新日期：1995-09-19 00:00:00

abstract：:The Rhodnius prolixus aggregation inhibitor 1 (RPAI-1) is a novel blood-sucking salivary molecule that binds to ADP and attenuates platelet aggregation. In this report, we determine the binding constants and specificity of RPAI-1 for adenine nucleotides and its functional significance. By the Hummel-Dreyer method of e...

journal_title：Biochemistry

authors： Francischetti IM,Andersen JF,Ribeiro JM

更新日期：2002-03-19 00:00:00

abstract：:A particle obtained by nuclease digestion of nucleohistone complexes prepared by direct mixing of histones with DNA in 0.15 M NaCl was indistinguishable by composition and physical properties from nucleosome cores prepared under the same conditions from nucleohistone preannealed in 0.6 M NaCl. We show here that differ...

journal_title：Biochemistry

authors： Kahr WH,Lewis PN,Pulleyblank DE

更新日期：1990-06-19 00:00:00

abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...

journal_title：Biochemistry

authors： Quaye JA,Gadda G

更新日期：2020-12-29 00:00:00

abstract：:TAR DNA-binding protein 43 (TDP-43) has been identified as the major constituent of the proteinaceous inclusions that are characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin positive frontotemporal lobar degeneration (FTLD). Wild type TDP-43 inclusions are a pathological hallmark of >95% ...

journal_title：Biochemistry

authors： Ng JSW,Hanspal MA,Matharu NS,Barros TP,Esbjörner EK,Wilson MR,Yerbury JJ,Dobson CM,Kumita JR

更新日期：2019-10-01 00:00:00

abstract：:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...

journal_title：Biochemistry

authors： Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

更新日期：2005-06-21 00:00:00

abstract：:Glycosylases specifically recognize and flip their target base out of the DNA helix into the enzyme's active site. Our simulations show that a partially flipped state, already present in free DNA carrying a T:G mispair, becomes the more probable state compared to the closed state after binding of thymine DNA glycosyla...

journal_title：Biochemistry

authors： Kanaan N,Imhof P

更新日期：2018-10-02 00:00:00

abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

journal_title：Biochemistry

authors： Loret EP,Mansuelle P,Rochat H,Granier C

更新日期：1990-02-13 00:00:00

abstract：:The existence of two conformational states of concanavalin A (Con A) with different metal ion binding properties has been recently demonstrated (Brown, R. D., Brewer, C. F., & Koenig, S. H. (1977) Biochemistry 16, 3883). Introduction of Mn2+ to the S1 site and Ca2+ to the S2 site of apo-Con A was shown to induce a con...

journal_title：Biochemistry

authors： Koenig SH,Brewer CF,Brown RD 3rd

更新日期：1978-10-03 00:00:00

abstract：:The active site of histidine decarboxylase (HDC) from Lactobacillus 30a contains a pyruvoyl cofactor sitting at the interface of two molecules in a trimer. Although exhibiting hyperbolic kinetics at pH 4.8, near its optimum, HDC is cooperative at pH 7.6, indicating that the units of the trimer communicate. A Hill plot...

journal_title：Biochemistry

authors： Pishko EJ,Robertus JD

更新日期：1993-05-11 00:00:00

abstract：:The structure of an analogue of the yeast tRNAPhe T Psi C stem-loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified T Psi C stem-loop is the product of the m...

journal_title：Biochemistry

authors： Koshlap KM,Guenther R,Sochacka E,Malkiewicz A,Agris PF

更新日期：1999-07-06 00:00:00

abstract：:Mutations in the gene encoding human ATP:cobalamin adenosyltransferase (hATR) can result in the metabolic disorder known as methylmalonic aciduria (MMA). This enzyme catalyzes the final step in the conversion of cyanocobalamin (vitamin B12) to the essential human cofactor adenosylcobalamin. Here we present the 2.5 A c...

journal_title：Biochemistry

authors： Schubert HL,Hill CP

更新日期：2006-12-26 00:00:00

abstract：:We previously reported that enzymatically modified low-density lipoprotein (E-LDL) particles obtained by LDL treatment with trypsin and then cholesterol esterase are recognized by C1q and activate the C1 complex of complement. The objective of this study was to identify the E-LDL component(s) recognized by C1q. In add...

journal_title：Biochemistry

authors： Biro A,Ling WL,Arlaud GJ

更新日期：2010-03-16 00:00:00

abstract：:Calmodulin-dependent protein phosphatase, one of the major calmodulin-binding proteins in bovine brain, dephosphorylates casein with a specific activity of 15 nmol mg-1 min-1 at 30 degrees C. The stimulation of phosphatase activity by calmodulin is reversed by ethylene glycol bis (beta-aminoethyl ether)-N,N,N',N'-tetr...

journal_title：Biochemistry

authors： Tallant EA,Cheung WY

更新日期：1983-07-19 00:00:00

abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

journal_title：Biochemistry

authors： Weidman SW,Drysdale GR

更新日期：1979-08-21 00:00:00

abstract：:Extracts of two human glioma cell lines (lacking O6-methylguanine DNA-methyltransferase) (i.e., A1235 and its alkylation-resistant derivative A1235-MR4) were examined for their ability to execute strand incision at different base mismatches in model (45-bp) DNA. These heteroduplex substrates were of the same sequence ...

journal_title：Biochemistry

authors： Lari SU,Al-Khodairy F,Paterson MC

更新日期：2002-07-23 00:00:00

abstract：:The GTP-binding proteins or GTPases comprise a superfamily of proteins that provide molecular switches in numerous cellular processes. The "GTPase switch" paradigm, in which a GTPase acts as a bimodal switch that is turned "on" and "off" by external regulatory factors, has been used to interpret the regulatory mechani...

journal_title：Biochemistry

authors： Shan SO,Schmid SL,Zhang X

更新日期：2009-07-28 00:00:00

abstract：:Carboxyl ester lipase (CEL; EC 3.1.1.13) hydrolyzes cholesteryl esters and retinyl esters in vitro. In vivo, pancreatic CEL is thought to liberate cholesterol and retinol from their esters prior to absorption in the intestine. CEL is also a major lipase in the breast milk of many mammals, including humans and mice, an...

journal_title：Biochemistry

authors： Weng W,Li L,van Bennekum AM,Potter SH,Harrison EH,Blaner WS,Breslow JL,Fisher EA

更新日期：1999-03-30 00:00:00

abstract：:The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...

journal_title：Biochemistry

authors： Streeter CC,Lin Q,Firestine SM

更新日期：2019-04-30 00:00:00

abstract：:The equilibrium binding and association kinetics of the Saccharomyces cerevisiae TATA Binding Protein (TBP) to the E4 and Major Late promoters of adenovirus (TATATATA and TATAAAAG, respectively), have been directly compared by quantitative DNase I titration and quench-flow "footprinting". The equilibrium binding of TB...

journal_title：Biochemistry

authors： Petri V,Hsieh M,Jamison E,Brenowitz M

更新日期：1998-11-10 00:00:00

abstract：:The ferlin family proteins have emerged as multi-C2 domain regulators of calcium-triggered membrane fusion and fission events. While initially determined to share many of the features of members of the synaptotagmin family of calcium sensors, ferlins in more recent studies have been found to interact directly with non...

journal_title：Biochemistry

authors： Johnson CP

更新日期：2017-12-12 00:00:00

abstract：:The sucrose permease (CscB) and lactose permease (LacY) of Escherichia coli belong to the oligosaccharide/H(+) symporter subfamily of the major facilitator superfamily, and both catalyze sugar/H(+) symport across the cytoplasmic membrane. Thus far, there is no common substrate for the two permeases; CscB transports su...

journal_title：Biochemistry

authors： Sugihara J,Smirnova I,Kasho V,Kaback HR

更新日期：2011-12-27 00:00:00

abstract：:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detai...

journal_title：Biochemistry

authors： Tse Sum Bui B,Benda R,Schünemann V,Florentin D,Trautwein AX,Marquet A

更新日期：2003-07-29 00:00:00

abstract：:The effects of NH3 on the oxygen evolving enzyme have been investigated with EPR and steady-state O2 evolution. The following results were obtained. At low light intensity O2 evolution occurs in all centers even though ammonia is bound. This binding occurs in the S2 state and results in a modification of the multiline...

journal_title：Biochemistry

authors： Boussac A,Rutherford AW,Styring S

更新日期：1990-01-09 00:00:00

abstract：:Hepatic microsomal NADPH-cytochrome P-450 reductase was solubilized from rabbit liver microsomes in the presence of detergents and purified to homogeneity by column chromatography. The purified reductase had a molecular weight of 78 000 and contained 1 mol each of FAD and FMN per mol of enzyme. On reduction with NADPH...

journal_title：Biochemistry

authors： Iyanagi T,Anan FK,Imai Y,Mason HS

更新日期：1978-05-30 00:00:00