Abstract:
:tRNase Z, which exists in almost all cells, is believed to be working primarily for tRNA 3' maturation. In Escherichia coli, however, the tRNase Z gene appears to be dispensable under normal growth conditions, and its physiological role is not clear. Here, to investigate a possibility that E. coli tRNase Z cleaves RNAs other than pre-tRNAs, we tested several unstructured RNAs for cleavage. Surprisingly, all these substrates were cleaved very efficiently at multiple sites by a recombinant E. coli enzyme in vitro. tRNase Zs from Bacillus subtilis and Thermotoga maritima also cleaved various unstructured RNAs. The E. coli and B. subtilis enzymes seem to have a tendency to cleave after cytidine or before uridine, while cleavage by the T. maritima enzyme inevitably occurred after CCA in addition to the other cleavages. Assays to determine optimal conditions indicated that metal ion requirements differ between B. subtilis and T. maritima tRNase Zs. There was no significant difference in the observed rate constant between unstructured RNA and pre-tRNA substrates, while the K(d) value of a tRNase Z/unstructured RNA complex was much higher than that of an enzyme/pre-tRNA complex. Furthermore, eukaryotic tRNase Zs from yeast, pig, and human cleaved unstructured RNA at multiple sites, but an archaeal tRNase Z from Pyrobaculum aerophilum did not.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Shibata HS,Minagawa A,Takaku H,Takagi M,Nashimoto Mdoi
10.1021/bi051972ssubject
Has Abstractpub_date
2006-05-02 00:00:00pages
5486-92issue
17eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Overall activation volumes for multistep reactions are not usually pressure independent. The present investigation gives a quantitative description of this effect under Theory. Simple relations are obtained which can easily be applied to experimental data and which allow more insight into the dynamics of enzyme reacti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00405a024
更新日期:1988-03-08 00:00:00
abstract::The pre-steady-state kinetics of the trichodiene synthase reaction were investigated by rapid chemical quench methods. The single-turnover rate was found to be 3.5-3.8 s-1, a rate 40 times faster than the steady-state catalytic rate (kcat = 0.09 s-1) for trichodiene synthase-catalyzed conversion of farnesyl diphosphat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963018o
更新日期:1997-07-08 00:00:00
abstract::Cytochrome b5 reductase (cb5r), a member of the ferredoxin:NADP+ reductase family of flavoprotein transhydrogenases, catalyzes the NADH-dependent reduction of cytochrome b5. Within this family, a conserved "GxGxxP" sequence motif has been implicated in binding reduced pyridine nucleotides. However, Glycine 179, a cons...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051165t
更新日期:2005-10-18 00:00:00
abstract::Uncontrolled proteolysis due to cathepsin G (cat G) may cause severe pathological disorders. Cat G is inhibited by alpha 1-antichymotrypsin (ACT) and alpha 1-proteinase inhibitor (alpha 1PI), two members of the serpin superfamily of proteins. To see whether these two inhibitors play a physiological proteolysis-prevent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980223q
更新日期:1998-08-11 00:00:00
abstract::Pepsin, a gastric aspartic proteinase, is a zymogen-derived protein that undergoes irreversible alkaline denaturation at pH 6-7. Detailed knowledge of the structure of the alkaline-denatured state is an important step in understanding the mechanism of the formation of the active enzyme. An extensive analysis of the de...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991923d
更新日期:2000-04-11 00:00:00
abstract::Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a018
更新日期:1987-06-30 00:00:00
abstract::Rhodobacter sphaeroides strains lacking cytochrome c2 (cyt c2), the normal electron donor to P870+ in light-oxidized reaction center (RC) complexes, are unable to grow photosynthetically. However, spd mutations that suppress the photosynthetic deficiency of cyt c2 mutants elevate levels of the cyt c2 isoform, isocyt c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961648k
更新日期:1997-01-28 00:00:00
abstract::The binding of human Glu- and Lys-plasminogens to intact fibrin clots, to lysine-Sepharose, and to fibrin cleaved by plasmin was quantitatively characterized. On intact fibrin clots, there was one strong binding site for Glu-plasminogen with a dissociation constant, Kd, of 25 microM and one strong binding site for Lys...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00334a031
更新日期:1985-06-18 00:00:00
abstract::Salmonella typhimurium L-histidinol dehydrogenase (EC 1.1.1.23), a four-electron dehydrogenase, was inactivated by an active-site-directed modification reagent, 7-chloro-4-nitro-2,1,3-benzoxadiazole (NBD-Cl). The inactivation followed pseudo-first-order kinetics and was prevented by low concentrations of the substrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00365a009
更新日期:1986-08-26 00:00:00
abstract::A new class of bacterial multisubunit membrane-bound electron-transfer complexes has been identified based on biochemical and bioinformatic data. It contains subunits homologous to the three-subunit molybdopterin oxidoreductases and four additional subunits, two of which are c-type cytochromes. The complex was purifie...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047267l
更新日期:2005-08-02 00:00:00
abstract::The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1020106
更新日期:2011-04-05 00:00:00
abstract::The newly discovered oncogenic protein gankyrin, which contains six ankyrin repeats, has been reported to be involved in the phosphorylation and degradation of the retinoblastoma gene product, Rb. Using in vitro systems, we have identified a peptide fragment of gankyrin, 176LHLACDEERN185, which is responsible for bind...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011550s
更新日期:2002-03-26 00:00:00
abstract::The calcium sensitivity of vertebrate skeletal muscle myosin has been investigated. Adenosinetriphosphatase (ATPase) activity was assayed in a reconstituted system composed of either purified rabbit myosin plus actin or myosin plus actin, tropomyosin, and troponin. The calcium sensitivity of actomyosin Mg-ATPase activ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00279a004
更新日期:1983-05-10 00:00:00
abstract::Bacteriocins are bacterial peptides with specific activity against competing species. They hold great potential as natural preservatives and for their probiotic effects. We show here nuclear magnetic resonance-based evidence that glycocin F, a 43-amino acid bacteriocin from Lactobacillus plantarum, contains two β-link...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200217u
更新日期:2011-04-12 00:00:00
abstract::The effect of pretreatment with sodium butyrate on DNA excision repair was studied in intact and permeable confluent (i.e., growth-inhibited) diploid human fibroblasts. Exposure to 20 mM sodium butyrate for 48 h increased subsequent ultraviolet (UV)-induced [methyl-3H]thymidine incorporation by intact AG1518 fibroblas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00345a019
更新日期:1985-11-19 00:00:00
abstract::The collagens synthesized by Chinese hamster ovary cells have been isolated and characterized. Although these cells produce very small amounts of collagen, at least five distinct collagenous chains could be identified from radiolabeled media and cell extracts after limited pepsin digestion. Two chains were characteriz...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00262a031
更新日期:1982-09-14 00:00:00
abstract::Reactive metabolites of environmental chemicals and drugs can cause site specific damage to the p53 tumor suppressor gene in a major pathway for genotoxicity. We report here a high-throughput, cell-free, 96-well plate magnetic bead-enzyme system interfaced with LC-MS/MS sequencing for bioactivating test chemicals and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00271
更新日期:2018-07-03 00:00:00
abstract::Regulation of Saccharomyces cerevisiae membrane-associated phosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids was examined using purified enzyme and Triton X-100/phospholipid-mixed micelles. Anionic phospholipids activated phosphatidate phosphatase activity whereas zw...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952808f
更新日期:1996-03-26 00:00:00
abstract::We detect internal water molecules in a membrane-embedded receptor-transducer complex and demonstrate water structure changes during formation of the signaling state. Time-resolved FTIR spectroscopy reveals stimulus-induced repositioning of one or more structurally active water molecules to a significantly more hydrop...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802180a
更新日期:2009-02-10 00:00:00
abstract::Both metal and flavin-dependent sulfhydryl oxidases catalyze the net generation of disulfide bonds with the reduction of oxygen to hydrogen peroxide. The first mammalian sulfhydryl oxidase to be described was an iron-dependent enzyme isolated from bovine milk whey (Janolino, V.G., and Swaisgood, H.E. (1975) J. Biol. C...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7016975
更新日期:2007-11-13 00:00:00
abstract::Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00691a008
更新日期:1975-10-07 00:00:00
abstract::One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. The protein appears to interact with its substrates within the membrane enviro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0485326
更新日期:2005-01-18 00:00:00
abstract::Triclinic crystals of hen egg-white lysozyme cross-linked with glutaraldehyde have been treated with various denaturants and found to be susceptible to x-ray structure analysis even after major conformational changes in the protein. Cross-linked crystals were isomorphous with the native form, and electron density diff...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a027
更新日期:1977-04-05 00:00:00
abstract::The gamma subunits of the 7S nerve growth factor complex (7S NGF) display arginine esteropeptidase activity. By varying the conditions of electrophoresis in acrylamide gel, it has been demonstrated that the gamma-subunit fraction of 7S NGF contains five different proteins, in contrast to the three (gamma1, gamma2, and...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00652a016
更新日期:1976-04-06 00:00:00
abstract::Glycogen synthase kinase 3β (GSK-3β) is a serine-threonine kinase belonging to the CMGC family that plays a key role in many biological processes, such as glucose metabolism, cell cycle regulation, and proliferation. Like most protein kinases, GSK-3β is regulated via multiple pathways and sites. We performed all-atom ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100822q
更新日期:2010-12-28 00:00:00
abstract::Interconversion of protein conformations is imperative to function, as evidenced by conformational changes associated with enzyme catalytic cycles, ligand binding and post-translational modifications. In this study, we used 15N NMR relaxation experiments to probe the fast (i.e., ps-ns) and slow (i.e., micros-ms) confo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701425n
更新日期:2007-12-25 00:00:00
abstract::The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047918d
更新日期:2005-02-15 00:00:00
abstract::The structure of a mutant Escherichia coli thioredoxin with a glutamic acid substituted for a conserved lysine at position 36 adjacent to the active site has been solved using molecular replacement and refined at 2.0-A resolution to a crystallographic residual of 19.9%. The mutant was crystallized in an orthorhombic s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00070a017
更新日期:1993-05-18 00:00:00
abstract::Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00391a033
更新日期:1987-08-25 00:00:00
abstract::Azide binds to fast cytochrome bo with a stoichiometry of 1:1, the dissociation constant for this reaction being approximately 2 x 10(-5) M. The changes induced in the electronic absorption are very slight and are consistent with heme o remaining hexacoordinate high-spin, an observation confirmed by room temperature M...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961221d
更新日期:1996-10-29 00:00:00