Crystal structure analysis of a mutant Escherichia coli thioredoxin in which lysine 36 is replaced by glutamic acid.

abstract：:Ultrahigh-resolution X-ray diffraction data from cryo-cooled, B. lentus subtilisin crystals has been collected to a resolution of 0.78 A. The refined model coordinates have a rms deviation of 0.22 A relative to the same structure determined at room temperature and 2.0 A resolution. Several regions of main-chain and si...

journal_title：Biochemistry

authors： Kuhn P,Knapp M,Soltis SM,Ganshaw G,Thoene M,Bott R

更新日期：1998-09-29 00:00:00

abstract：:A galactoside-binding lectin (hL-31) containing a collagen-like sequence was identified in human tumor cells. It was found to be the homologue of the IgE-binding protein, the macrophage cell-surface Mac-2 antigen, and the murine CBP35, RL-29, and mL-34 lectins. Here we report on the expression in Escherichia coli and ...

journal_title：Biochemistry

authors： Ochieng J,Platt D,Tait L,Hogan V,Raz T,Carmi P,Raz A

更新日期：1993-04-27 00:00:00

abstract：:Thermodynamic data for RNA 1 x 2 nucleotide internal loops are lacking. Thermodynamic data that are available for 1 x 2 loops, however, are for loops that rarely occur in nature. In order to identify the most frequently occurring 1 x 2 nucleotide internal loops, a database of 955 RNA secondary structures was compiled ...

journal_title：Biochemistry

authors： Badhwar J,Karri S,Cass CK,Wunderlich EL,Znosko BM

更新日期：2007-12-18 00:00:00

abstract：:The inducible isoform of nitric oxide synthase (iNOS) and three zinc tetrathiolate mutants (C104A, C109A, and C104A/C109A) were expressed in Escherichia coli and purified. The mutants were found by ICP-AES and the zinc-specific PAR colorimetric assay to be zinc free, whereas the wild-type iNOS zinc content was 0.38 +/...

journal_title：Biochemistry

authors： Mitchell DA,Erwin PA,Michel T,Marletta MA

更新日期：2005-03-29 00:00:00

abstract：:Recombinant human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an aden...

journal_title：Biochemistry

authors： Lansdon EB,Fisher AJ,Segel IH

更新日期：2004-04-13 00:00:00

abstract：:The iron heme and its immediate environment can provide information that is pivotal to our understanding of the structural and mechanistic features that confer unusual properties to the heme peroxidases. X-ray absorption spectroscopy (XAS), which is ideally suited for the investigation of the local environment and ele...

journal_title：Biochemistry

authors： Sinclair R,Copeland B,Yamazaki I,Powers L

更新日期：1995-10-10 00:00:00

abstract：:Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6...

journal_title：Biochemistry

authors： Huddleston JP,Anderson TK,Spencer KD,Thoden JB,Raushel FM,Holden HM

更新日期：2020-04-07 00:00:00

abstract：:We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer in its native state. When BDNF unfolded in 6 M guanidine hydrochloride is diluted 20-fold into phosphate-buffered saline, a partially folded intermediate is rapi...

journal_title：Biochemistry

authors： Philo JS,Rosenfeld R,Arakawa T,Wen J,Narhi LO

更新日期：1993-10-12 00:00:00

abstract：:The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...

journal_title：Biochemistry

authors： Mizushina Y,Ohkubo T,Sugawara F,Sakaguchi K

更新日期：2000-10-17 00:00:00

abstract：:Vibrational circular dichroism (VCD) and Fourier transform IR (FTIR) were measured for a series of short alanine-based peptides having the general formula Ac-(AAKAA)n-GY-NH2 (n = 1-4) from 5 to 50 degrees C in D2O and at room temperature in both TFE and H2O. In both of these latter solvents, the dominant structural fo...

journal_title：Biochemistry

authors： Yoder G,Pancoska P,Keiderling TA

更新日期：1997-12-09 00:00:00

abstract：:We previously reported that the microtubule (MT)-binding domain of microtubule-associated protein 4 (MAP4) contains three 18-amino acid imperfect repeats that are homologous to the repeats found in the MT-binding domains of the neuronal MAPs, MAP2 and tau [Chapin, S. J., & Bulinski, J. C. (1991) J. Cell Sci. 98, 27-36...

journal_title：Biochemistry

authors： Chapin SJ,Lue CM,Yu MT,Bulinski JC

更新日期：1995-02-21 00:00:00

abstract：:Protein enzymes often use ionizable side chains, such as histidine, for general acid-base catalysis because the imidazole pK(a) is near neutral pH. RNA enzymes, on the other hand, are comprised of nucleotides which do not have apparent pK(a) values near neutral pH. Nevertheless, it has been recently shown that cytidin...

journal_title：Biochemistry

authors： Ravindranathan S,Butcher SE,Feigon J

更新日期：2000-12-26 00:00:00

abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...

journal_title：Biochemistry

authors： Moore RA,Hayes SF,Fischer ER,Priola SA

更新日期：2007-06-19 00:00:00

abstract：:Heparin accelerates inhibition of factor XIa (fXIa) by the serpins antithrombin (AT) and C1-inhibitor (C1-INH) by more than 2 orders of magnitude. The mechanism of the heparin-mediated acceleration of fXIa inhibition by these serpins is incompletely understood, as heparin appears to interact with both the catalytic an...

journal_title：Biochemistry

authors： Yang L,Sun MF,Gailani D,Rezaie AR

更新日期：2009-02-24 00:00:00

abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...

journal_title：Biochemistry

authors： Li H,Wang HY,Kang S,Silverman RB,Poulos TL

更新日期：2016-07-05 00:00:00

abstract：:Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...

journal_title：Biochemistry

authors： Sali DL,Ingram R,Wendel M,Gupta D,McNemar C,Tsarbopoulos A,Chen JW,Hong Z,Chase R,Risano C,Zhang R,Yao N,Kwong AD,Ramanathan L,Le HV,Weber PC

更新日期：1998-03-10 00:00:00

abstract：:The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

journal_title：Biochemistry

authors： Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

更新日期：2010-01-26 00:00:00

abstract：:The natural product hypericin is a photosensitive polycyclic aromatic dione compound, which has been widely investigated because of its virucidal and antitumor properties. Although it has been suggested that singlet oxygen or a radical species might be responsible for its biological action, its mechanism of action rem...

journal_title：Biochemistry

authors： Weiner L,Roth E,Mazur Y,Silman I

更新日期：1999-08-31 00:00:00

abstract：:Proteins have many distinct tertiary folds (Richardson, J. S. (1981) Adv. Prot. Chem. 34, 167-339). The term tertiary fold refers to the spatial organization of secondary structure elements (alpha-helices and beta-strands). It is not known when, in the process of protein folding, a native tertiary fold emerges. Here, ...

journal_title：Biochemistry

authors： Peng ZY,Kim PS

更新日期：1994-03-01 00:00:00

abstract：:Clip domains are structural modules found in arthropod serine proteinases and some proteolytically inactive homologues, which mediate extracellular signaling pathways of development and immunity. While little is known about their structures or functions, clip domains are proposed to be sites for interactions of protei...

journal_title：Biochemistry

authors： Huang R,Lu Z,Dai H,Velde DV,Prakash O,Jiang H

更新日期：2007-10-16 00:00:00

abstract：:During the past decade, there has been much debate about the enormous catalytic rate enhancement observed in enzymatic reactions involving carbanion intermediates. Our recent theoretical study has demonstrated the importance of the short strong hydrogen bond (SSHB) in the enzymatic reactions. Nevertheless, other recen...

journal_title：Biochemistry

authors： Kim KS,Kim D,Lee JY,Tarakeshwar P,Oh KS

更新日期：2002-04-23 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...

journal_title：Biochemistry

authors： Tanner KG,Langer MR,Denu JM

更新日期：2000-10-03 00:00:00

abstract：:Each homologous lobe of human serum transferrin (hTF) has one Fe(3+) ion bound by an aspartic acid, a histidine, two tyrosine residues, and two oxygens from the synergistic anion, carbonate. Extensive characterization of these ligands in the N-terminal lobe has been carried out. Despite sharing the same set of ligands...

journal_title：Biochemistry

authors： Mason AB,Halbrooks PJ,James NG,Connolly SA,Larouche JR,Smith VC,MacGillivray RT,Chasteen ND

更新日期：2005-06-07 00:00:00

abstract：:We have investigated the denaturation by guanidine hydrochloride (Gdn X HCl), temperature, and pH of hemoglobin beta subunits and of the peptides beta (1-146), beta (56-146), and beta (1-55). The last peptide was insensitive to all of the three agents. In the other polypeptides denaturation by Gdn X HCl and temperatur...

journal_title：Biochemistry

authors： Franchi D,Fronticelli C,Bucci E

更新日期：1982-11-23 00:00:00

abstract：:Three different cleavable photoactivable heterobifunctional reagents have been synthesized and characterized by proton NMR, 13C NMR, mass spectroscopy, infrared spectroscopy, and thin-layer chromatography. They are the N-hydroxysuccinimide ester of 3-[(4-azidophenyl)dithio]-propionic acid, methyl 3-[(4-azidophenyl)dit...

journal_title：Biochemistry

authors： Vanin EF,Ji TH

更新日期：1981-11-24 00:00:00

abstract：:The nerve growth factors (NGF's) isolated from cobra venom (Naja naja) and mouse submaxillary gland are closely related proteins. They are structurally similar in that about 60% of their amino acid residues are identical (Hogue-Angeletti, R. A., Frazier, W. A., Jacobs, J. W., Niall, H.D., and Bradshaw, R. A. (1976), B...

journal_title：Biochemistry

authors： Server AC,Herrup K,Shooter EM,Hogue-Angeletti RA,Frazier WA,Bradshaw RA

更新日期：1976-01-13 00:00:00

abstract：:Heme propionate-7 in cytochrome c is an ionizable group located in a region of the protein that is inaccessible to bulk solvent. Electrostatic stabilization of this functional group appears to be achieved through interaction of heme propionate-7 with several amino acid residues that occur within hydrogen-bonding dista...

journal_title：Biochemistry

authors： Davies AM,Guillemette JG,Smith M,Greenwood C,Thurgood AG,Mauk AG,Moore GR

更新日期：1993-05-25 00:00:00

abstract：:In this paper the interaction of cytoplasmic CopZ and the N-terminal domain of the CopA ATPase from Bacillus subtilis has been studied by NMR through (15)N-(1)H HSQC experiments in order to understand the role of the two proteins in the whole copper trafficking mechanism of the bacteria. It appears that the two protei...

journal_title：Biochemistry

authors： Banci L,Bertini I,Ciofi-Baffoni S,Del Conte R,Gonnelli L

更新日期：2003-02-25 00:00:00

abstract：:The properties of neuronal nitric oxide synthase containing one tetrahydrobiopterin (BH4) per dimer [nNOS(BH4+)] were compared to those of the BH4-free enzyme [nNOS(BH4-)]. The stimulation by BH4 of the formation of L-citrulline at the expense of H2O2 production unambiguously demonstrated that BH4 is essential in coup...

journal_title：Biochemistry

authors： Gorren AC,List BM,Schrammel A,Pitters E,Hemmens B,Werner ER,Schmidt K,Mayer B

更新日期：1996-12-24 00:00:00