当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Structural Analysis of Cj1427, an Essential NAD-Dependent Dehydrogenase for the Biosynthesis of the Heptose Residues in the Capsular Polysaccharides of Campylobacter jejuni.

Structural Analysis of Cj1427, an Essential NAD-Dependent Dehydrogenase for the Biosynthesis of the Heptose Residues in the Capsular Polysaccharides of Campylobacter jejuni.

Abstract：

:Many strains of Campylobacter jejuni display modified heptose residues in their capsular polysaccharides (CPS). The precursor heptose was previously shown to be GDP-d-glycero-α-d-manno-heptose, from which a variety of modifications of the sugar moiety have been observed. These modifications include the generation of 6-deoxy derivatives and alterations of the stereochemistry at C3-C6. Previous work has focused on the enzymes responsible for the generation of the 6-deoxy derivatives and those involved in altering the stereochemistry at C3 and C5. However, the generation of the 6-hydroxyl heptose residues remains uncertain due to the lack of a specific enzyme to catalyze the initial oxidation at C4 of GDP-d-glycero-α-d-manno-heptose. Here we reexamine the previously reported role of Cj1427, a dehydrogenase found in C. jejuni NTCC 11168 (HS:2). We show that Cj1427 is co-purified with bound NADH, thus hindering catalysis of oxidation reactions. However, addition of a co-substrate, α-ketoglutarate, converts the bound NADH to NAD+. In this form, Cj1427 catalyzes the oxidation of l-2-hydroxyglutarate back to α-ketoglutarate. The crystal structure of Cj1427 with bound GDP-d-glycero-α-d-manno-heptose shows that the NAD(H) cofactor is ideally positioned to catalyze the oxidation at C4 of the sugar substrate. Additionally, the overall fold of the Cj1427 subunit places it into the well-defined short-chain dehydrogenase/reductase superfamily. The observed quaternary structure of the tetrameric enzyme, however, is highly unusual for members of this superfamily.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Huddleston JP,Anderson TK,Spencer KD,Thoden JB,Raushel FM,Holden HM

doi

10.1021/acs.biochem.0c00096

subject

Has Abstract

pub_date

2020-04-07 00:00:00

pages

1314-1327

issue

13

eissn

0006-2960

issn

1520-4995

journal_volume

59

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

    abstract：:The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a021

    authors： Sims PJ,Wiedmer T

    更新日期：1984-07-03 00:00:00

  • Dihydrofolate reductase from Escherichia coli: probing the role of aspartate-27 and phenylalanine-137 in enzyme conformation and the binding of NADPH.

    abstract：:In the absence of ligands, dihydrofolate reductase from Escherichia coli exists in at least two interconvertible conformations, only one of which binds NADPH with high affinity. This equilibrium is pH dependent, involving an ionizable group of the enzyme (pK approximately 5.5), and the proportion of the NADPH-binding ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00489a010

    authors： Dunn SM,Lanigan TM,Howell EE

    更新日期：1990-09-18 00:00:00

  • Discovery and characterization of cooperative ligand binding in the adaptive region of interleukin-2.

    abstract：:The cytokine hormone interleukin-2 (IL-2) contains a highly adaptive region that binds small, druglike molecules. The binding properties of this adaptive region have been explored using a "tethering" method that relies on the formation of a disulfide bond between the protein and small-molecule ligands. Using tethering...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034138g

    authors： Hyde J,Braisted AC,Randal M,Arkin MR

    更新日期：2003-06-03 00:00:00

  • Crystal structures of thrombin complexed to a novel series of synthetic inhibitors containing a 5,5-trans-lactone template.

    abstract：:The binding modes of four active site-directed, acylating inhibitors of human alpha-thrombin have been determined using X-ray crystallography. These inhibitors (GR157368, GR166081, GR167088, and GR179849) are representatives of a series utilizing a novel 5, 5-trans-lactone template to specifically acylate Ser195 of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9830359

    authors： Jhoti H,Cleasby A,Reid S,Thomas PJ,Weir M,Wonacott A

    更新日期：1999-06-22 00:00:00

  • Interaction of divalent cations with beta-galactosidase (Escherichia coli).

    abstract：:Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00586a005

    authors： Huber RE,Parfett C,Woulfe-Flanagan H,Thompson DJ

    更新日期：1979-09-18 00:00:00

  • Identification of potential regulatory sites of the Na+,K+-ATPase by kinetic analysis.

    abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0355443

    authors： Kong BY,Clarke RJ

    更新日期：2004-03-02 00:00:00

  • Structural RNA mimetics: N3'-->P5' phosphoramidate DNA analogs of HIV-1 RRE and TAR RNA form A-type helices that bind specifically to Rev and Tat-related peptides.

    abstract：:An attractive strategy for the development of anti-retroviral drugs is the exploration of compounds that mimic RNA control regions of the viral genome and act as "decoys" to sequester viral gene regulatory proteins. Decoys consisting of RNA, however, are chemically unstable and readily degraded by cellular nucleases. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961980w

    authors： Rigl CT,Lloyd DH,Tsou DS,Gryaznov SM,Wilson WD

    更新日期：1997-01-21 00:00:00

  • Evolutionary conservation of enzymatic catalysis: quantitative comparison of the effects of mutation of aligned residues in Saccharomyces cerevisiae and Escherichia coli inorganic pyrophosphatases on enzymatic activity.

    abstract：:Soluble inorganic pyrophosphatase (PPase) is one of the better understood phosphoryl-transfer enzymes and is distinctive in having four divalent metal ions at the active site. Here we determine pH profiles for wild-type Saccharomyces cerevisiae PPase (Y-PPase) and for 14 of its active site variants and consider the ef...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971771r

    authors： Pohjanjoki P,Lahti R,Goldman A,Cooperman BS

    更新日期：1998-02-17 00:00:00

  • Fast cytochrome bo from Escherichia coli binds two molecules of nitric oxide at CuB.

    abstract：:The reaction of nitric oxide (NO) with fast cytochrome bo from Escherichia coli has been studied by electronic absorption, MCD, and EPR spectroscopy. Titration of the enzyme with NO showed the formation of two distinct species, consistent with NO binding stoichiometries of 1:1 and 2:1 with observed dissociation consta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971481a

    authors： Butler CS,Seward HE,Greenwood C,Thomson AJ

    更新日期：1997-12-23 00:00:00

  • Mechanism and role of cooperative binding of bacteriophage fd gene 5 protein to single-stranded deoxyribonucleic acid.

    abstract：:The highly cooperative binding of fd gene 5 to single-stranded DNA was studied kinetically by rapid photo-cross-linking and stopped-flow UV absorption measurements. The observed change in absorbance was shown to be due to the binding by direct evidence of rapid photo-cross-linking of the bound proteins to fd DNA. The ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00294a028

    authors： Shimamoto N,Utiyama H

    更新日期：1983-12-06 00:00:00

  • Bile acid/phosphatidylcholine interactions in mixed monomolecular layers: differences in condensation effects but not interfacial orientation between hydrophobic and hydrophilic bile acid species.

    abstract：:Monomolecular layers of undissociated bile acids and membrane lipids at the air/water interface serve as useful models for the interactions between fully ionized bile salts and physiological membranes. Employing an automated Langmuir-Pockels surface balance, surface pressures and dipole moments were measured as functi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00034a023

    authors： Fahey DA,Carey MC,Donovan JM

    更新日期：1995-08-29 00:00:00

  • Effect of subunit III removal on control of cytochrome c oxidase activity by pH.

    abstract：:Studies were undertaken to assess the postulated involvement of subunit III in the proton-linked functions of cytochrome c oxidase. The effect of pH on the steady-state kinetic [corrected] parameters of subunit III containing and subunit III depleted cytochrome oxidase was determined by using beef heart and rat liver ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00417a016

    authors： Gregory LC,Ferguson-Miller S

    更新日期：1988-08-23 00:00:00

  • Structural details on the binding of antihypertensive drugs captopril and enalaprilat to human testicular angiotensin I-converting enzyme.

    abstract：:Angiotensin converting enzyme (ACE) plays a critical role in the circulating or endocrine renin-angiotensin system (RAS) as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle. Here we report the high-resolution crystal structures of testis ACE (tACE) in complex with the firs...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049480n

    authors： Natesh R,Schwager SL,Evans HR,Sturrock ED,Acharya KR

    更新日期：2004-07-13 00:00:00

  • Consequences of single-site mutations in the intestinal fatty acid binding protein.

    abstract：:The intestinal fatty acid binding protein (IFABP) is a small (15 kDa) protein consisting mostly of 10 antiparallel beta-strands (A-J) and a small helical region that serves as a portal for the ligand. Two beta-sheet structures (strands A-E and F-J) surround a cavity into which the ligand binds. In this work, we invest...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0301688

    authors： Rajabzadeh M,Kao J,Frieden C

    更新日期：2003-10-28 00:00:00

  • Heterologous Expression, Purification, and Functional Analysis of Plasmodium falciparum Phosphatidylinositol 3'-Kinase.

    abstract：:The Plasmodium falciparum malarial parasite genome appears to encode one and only one phosphatidylinositol 3'-kinase (PI3K), and sequence analysis suggests that the enzyme is a "class III"- or "Vps34"-type PI3K. PfVps34 has generated excitement as a possible druggable target and potentially a key target of artemisinin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00416

    authors： Hassett MR,Sternberg AR,Riegel BE,Thomas CJ,Roepe PD

    更新日期：2017-08-22 00:00:00

  • Two-stage maturation process for newly replicated chromatin.

    abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00302a036

    authors： Smith PA,Jackson V,Chalkley R

    更新日期：1984-03-27 00:00:00

  • High-resolution crystal structures of the lectin-like xylan binding domain from Streptomyces lividans xylanase 10A with bound substrates reveal a novel mode of xylan binding.

    abstract：:Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015865j

    authors： Notenboom V,Boraston AB,Williams SJ,Kilburn DG,Rose DR

    更新日期：2002-04-02 00:00:00

  • Role of alpha-synuclein carboxy-terminus on fibril formation in vitro.

    abstract：:Alpha-synuclein (alpha-syn) is the major component of intracellular inclusions in several neurodegenerative diseases, and the conversion of soluble alpha-syn into filamentous aggregates may contribute to disease pathogenesis. Since mechanisms leading to the formation of alpha-syn inclusions are unclear, in vitro model...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi027363r

    authors： Murray IV,Giasson BI,Quinn SM,Koppaka V,Axelsen PH,Ischiropoulos H,Trojanowski JQ,Lee VM

    更新日期：2003-07-22 00:00:00

  • Spectroscopic and functional characterization of T state hemoglobin conformations encapsulated in silica gels.

    abstract：:Oxygen binding curves of sol-gel-encapsulated deoxy human adult hemoglobin (HbA) have previously revealed two distinct noncooperative populations with oxygen binding affinities approximately 1000 and 100 times lower than that of the high-affinity R state. The two populations which have been termed the low-affinity (LA...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048531d

    authors： Samuni U,Dantsker D,Juszczak LJ,Bettati S,Ronda L,Mozzarelli A,Friedman JM

    更新日期：2004-11-02 00:00:00

  • Crystal structures of metyrapone- and phenylimidazole-inhibited complexes of cytochrome P-450cam.

    abstract：:The crystal structures of metyrapone- and 1-, 2-, and 4-phenylimidazole-inhibited complexes of cytochrome P-450cam have been refined to a nominal resolution of 2.1 A and compared with the 1.63-A camphor-bound structure. With the exception of 2-phenylimidazole, each of the inhibitors forms an N-Fe bond with the heme ir...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00399a022

    authors： Poulos TL,Howard AJ

    更新日期：1987-12-15 00:00:00

  • Next-Generation Peptide Nucleic Acid Chimeras Exhibit High Affinity and Potent Gene Silencing.

    abstract：:We present a new design of mixed-backbone antisense oligonucleotides (ASOs) containing both DNA and peptide nucleic acid (PNA). Previous generations of PNA-DNA chimeras showed low binding affinity, reducing their potential as therapeutics. The addition of a 5'-wing of locked nucleic acid as well as the combination of ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00827

    authors： Debacker AJ,Sharma VK,Meda Krishnamurthy P,O'Reilly D,Greenhill R,Watts JK

    更新日期：2019-02-12 00:00:00

  • Acylation of lysine 860 allows tight binding and cytotoxicity of Bordetella adenylate cyclase on CD11b-expressing cells.

    abstract：:The Bordetella adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) forms cation-selective membrane channels and delivers into the cytosol of target cells an adenylate cyclase domain (AC) that catalyzes uncontrolled conversion of cellular ATP to cAMP. Both toxin activities were previously shown to depend on post-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050459b

    authors： Masin J,Basler M,Knapp O,El-Azami-El-Idrissi M,Maier E,Konopasek I,Benz R,Leclerc C,Sebo P

    更新日期：2005-09-27 00:00:00

  • Conformation of valine side chains in ribonuclease T1 determined by NMR studies of homonuclear and heteronuclear 3J coupling constants.

    abstract：:A conformational analysis of the valine side chains of ribonuclease T1 (RNase T1) was performed using NMR spectroscopy, in particular homonuclear (1H, 1H and 13C, 13C) and heteronuclear (1H, 15N and 1H, 13C) vicinal spin-spin coupling constants as obtained from E.COSY-type NMR experiments. The coupling constants relat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00184a017

    authors： Karimi-Nejad Y,Schmidt JM,Rüterjans H,Schwalbe H,Greisinger C

    更新日期：1994-05-10 00:00:00

  • Substrate binding to the peripheral site of acetylcholinesterase initiates enzymatic catalysis. Substrate inhibition arises as a secondary effect.

    abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9813577

    authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

    更新日期：1999-01-05 00:00:00

  • Formation and reactions of the heme-dioxygen intermediate in the first and second steps of nitric oxide synthesis as studied by stopped-flow spectroscopy under single-turnover conditions.

    abstract：:To better understand the mechanism of nitric oxide (NO) synthesis, we studied conversion of N-hydroxy-L-arginine (NOHA) or L-arginine (Arg) to citrulline and NO under single-turnover conditions using the oxygenase domain of neuronal nitric oxide synthase (nNOSoxy) and rapid scanning stopped-flow spectroscopy. When ana...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9920228

    authors： Boggs S,Huang L,Stuehr DJ

    更新日期：2000-03-07 00:00:00

  • Kissing-loop model of HIV-1 genome dimerization: HIV-1 RNAs can assume alternative dimeric forms, and all sequences upstream or downstream of hairpin 248-271 are dispensable for dimer formation.

    abstract：:The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5' end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization [Laughrea, M., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951838f

    authors： Laughrea M,Jetté L

    更新日期：1996-02-06 00:00:00

  • Influence of lipid composition on physical properties and peg-mediated fusion of curved and uncurved model membrane vesicles: "nature's own" fusogenic lipid bilayer.

    abstract：:Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002030k

    authors： Haque ME,McIntosh TJ,Lentz BR

    更新日期：2001-04-10 00:00:00

  • Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.

    abstract：:After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00581a015

    authors： Tanase S,Kojima H,Morino Y

    更新日期：1979-07-10 00:00:00

  • Substrate analogs as mechanistic probes for the bifunctional chorismate synthase from Neurospora crassa.

    abstract：:Analogs of EPSP (4-8) have been prepared, and their activity as substrates for the chorismate synthase from Neurospora crassa has been characterized kinetically. The enzyme appears to show strict discrimination against substitution at the Z-position of the enol ether side chain as well as against substitution at the S...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00251a019

    authors： Lauhon CT,Bartlett PA

    更新日期：1994-11-29 00:00:00

  • X-ray and EPR Characterization of the Auxiliary Fe-S Clusters in the Radical SAM Enzyme PqqE.

    abstract：:The Radical SAM (RS) enzyme PqqE catalyzes the first step in the biosynthesis of the bacterial cofactor pyrroloquinoline quinone, forming a new carbon-carbon bond between two side chains within the ribosomally synthesized peptide substrate PqqA. In addition to the active site RS 4Fe-4S cluster, PqqE is predicted to ha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01097

    authors： Barr I,Stich TA,Gizzi AS,Grove TL,Bonanno JB,Latham JA,Chung T,Wilmot CM,Britt RD,Almo SC,Klinman JP

    更新日期：2018-02-27 00:00:00