Discovery and characterization of cooperative ligand binding in the adaptive region of interleukin-2.

Abstract:

:The cytokine hormone interleukin-2 (IL-2) contains a highly adaptive region that binds small, druglike molecules. The binding properties of this adaptive region have been explored using a "tethering" method that relies on the formation of a disulfide bond between the protein and small-molecule ligands. Using tethering, surface plasmon resonance (SPR), and X-ray crystallography, we have discovered that the IL-2 adaptive region contains at least two cooperative binding sites where the binding of a first ligand to one site promotes or antagonizes the binding of a second ligand to the second site. Cooperative energies of interaction of -2 kcal/mol are observed. The observation that the adaptive region contains two adjacent sites may lead to the development of tight-binding antagonists of a protein-protein interaction. Cooperative ligand binding in the adaptive region of IL-2 underscores the importance of protein dynamics in molecular recognition. The tethering approach provides a novel and general strategy for discovering such cooperative binding interactions in specific, flexible regions of protein structure.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Hyde J,Braisted AC,Randal M,Arkin MR

doi

10.1021/bi034138g

subject

Has Abstract

pub_date

2003-06-03 00:00:00

pages

6475-83

issue

21

eissn

0006-2960

issn

1520-4995

journal_volume

42

pub_type

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