X-ray absorption spectroscopy comparison of the active site structures of Phanerochaete chrysosporium lignin peroxidase isoenzymes H2, H3, H4, H5, H8, and H10.

abstract：:We investigated the effects of substituting two of the four tryptophans (the "inner pair" Trp(9) and Trp(11) or the "outer pair" Trp(13) and Trp(15)) in gramicidin A (gA) channels. The conformational preferences of the doubly substituted gA analogues were assessed using circular dichroism spectroscopy and size-exclusi...

journal_title：Biochemistry

authors： Gu H,Lum K,Kim JH,Greathouse DV,Andersen OS,Koeppe RE 2nd

更新日期：2011-06-07 00:00:00

abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...

journal_title：Biochemistry

authors： Arregi I,Falces J,Bañuelos S,Urbaneja MA,Taneva SG

更新日期：2011-08-23 00:00:00

abstract：:The glucose derivative, 2,2,6,6-tetramethylpiperidine-1-oxylglucose (TEMPO-glucose) was synthesized and examined for its ability to substitute for glucose as a substrate for the intestinal brush border membrane Na+/glucose cotransporter. TEMPO-glucose inhibited Na(+)-dependent phlorizin binding with an apparent KI of ...

journal_title：Biochemistry

authors： Peerce BE

更新日期：1991-04-30 00:00:00

abstract：:Binding of streptokinase (SK) to plasminogen (Pg) activates the zymogen conformationally and initiates its conversion into the fibrinolytic proteinase, plasmin (Pm). Equilibrium binding studies of SK interactions with a homologous series of catalytic site-labeled fluorescent Pg and Pm analogues were performed to resol...

journal_title：Biochemistry

authors： Boxrud PD,Bock PE

更新日期：2000-11-14 00:00:00

abstract：:Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...

journal_title：Biochemistry

authors： Vashishtha AK,Konigsberg WH

更新日期：2016-05-10 00:00:00

abstract：:Drug substrates stimulate ATPase activity of the P-glycoprotein (P-gp) ATP-binding cassette drug pump by an unknown mechanism. Cross-linking analysis was performed to test if drug substrates stimulate P-gp ATPase activity by altering cross-talk at the first transmission interface linking the drug-binding [intracellula...

journal_title：Biochemistry

authors： Loo TW,Clarke DM

更新日期：2016-05-24 00:00:00

abstract：:A procedure has been developed to purify foot and mouth disease virus (FMDV) VP1 surface antigens from recombinant Escherichia coli. The VP1 antigens are expressed as fusion proteins derived from the E. coli Trp operon and VP1 surface protein of FMDV. The procedure is capable of recovering greater than 96% of the desi...

journal_title：Biochemistry

authors： Shire SJ,Bock L,Ogez J,Builder S,Kleid D,Moore DM

更新日期：1984-12-18 00:00:00

abstract：:The human acid sphingomyelinase (ASM, EC 3.1.4.12), a lysosomal and secretory protein coded by the sphingomyelin phosphodiesterase 1 (SMPD-1) gene, catalyzes the degradation of sphingomyelin (SM) to ceramide and phosphorylcholine. We examined the structural-functional properties of its carboxyl-terminus (amino acids 4...

journal_title：Biochemistry

authors： Lee CY,Tamura T,Rabah N,Lee DY,Ruel I,Hafiane A,Iatan I,Nyholt D,Laporte F,Lazure C,Wada I,Krimbou L,Genest J

更新日期：2007-12-25 00:00:00

abstract：:The homologous series of optically active short-chain phosphatidylethanolamines (PE) from dibutyryl-PE to dioctanoyl-PE was synthesized. In addition, two monomeric short-chain phospholipid analogues that are not degraded by phospholipase A2 (1,2-bis[(butylcarbamyl)oxy]-sn-glycero-3-phosphocholine and the corresponding...

journal_title：Biochemistry

authors： Plückthun A,Rohlfs R,Davidson FF,Dennis EA

更新日期：1985-07-16 00:00:00

abstract：:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...

journal_title：Biochemistry

authors： Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

更新日期：1985-11-05 00:00:00

abstract：:Cytochromes c require covalent attachment of heme via two thioether bonds at conserved CXXCH motifs, a process accomplished in prokaryotes by eight integral membrane proteins (CcmABCDEFGH), termed System I. Heme is trafficked from inside the cell to outside (via CcmABCD) and chaperoned (holoCcmE) to the cytochrome c s...

journal_title：Biochemistry

authors： Sutherland MC,Rankin JA,Kranz RG

更新日期：2016-06-07 00:00:00

abstract：:We found that peroxisomal lignoceroyl-CoA ligase, like palmitoyl-CoA ligase, is present in the peroxisomal membrane whereas the peroxisomal beta-oxidation enzyme system is localized in the matrix. To further define the role of peroxisomal acyl-CoA ligases (membrane component) in providing acyl-CoA for peroxisomal beta...

journal_title：Biochemistry

authors： Lazo O,Contreras M,Singh I

更新日期：1990-04-24 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:Translational initiation factor IF-2 is involved in a multistep pathway leading to the synthesis of the first peptide bond. IF-2 is a guanine nucleotide binding protein (G-protein) and catalyzes GTP hydrolysis in the presence of ribosomes. According to sequence homologies with other G-proteins, particularly EF-Tu, a t...

journal_title：Biochemistry

authors： Vachon G,Laalami S,Grunberg-Manago M,Julien R,Cenatiempo Y

更新日期：1990-10-16 00:00:00

abstract：:We detect internal water molecules in a membrane-embedded receptor-transducer complex and demonstrate water structure changes during formation of the signaling state. Time-resolved FTIR spectroscopy reveals stimulus-induced repositioning of one or more structurally active water molecules to a significantly more hydrop...

journal_title：Biochemistry

authors： Bergo VB,Spudich EN,Spudich JL,Rothschild KJ

更新日期：2009-02-10 00:00:00

abstract：:Previously, we have described a miniature protein-based approach to the design of molecules that bind DNA or protein surfaces with high affinity and specificity. In this approach, the small, well-folded protein avian pancreatic polypeptide acts as a scaffold to present and stabilize an alpha-helical or PPII-helical re...

journal_title：Biochemistry

authors： Yang L,Schepartz A

更新日期：2005-05-24 00:00:00

abstract：:The discovery of three mutants in the -synuclein (alphaSyn) gene and the identification of alphaSyn as the major component of Lewy body have opened a new field for understanding the pathogenesis of Parkinson's disease (PD). AlphaSyn is a natively unfolded protein with unknown function and unspecified conformational he...

journal_title：Biochemistry

authors： Jiang C,Chang JY

更新日期：2007-01-16 00:00:00

abstract：:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluoresce...

journal_title：Biochemistry

authors： Gorbunov NV,Osipov AN,Day BW,Zayas-Rivera B,Kagan VE,Elsayed NM

更新日期：1995-05-23 00:00:00

abstract：:Helicases are enzymes that use energy from nucleoside triphosphate hydrolysis to unwind double-stranded (ds) DNA, a process vital to virtually every phase of DNA metabolism. Helicases have been classified as either 5'-to-3' or 3'-to-5' on the basis of their ability to unwind duplex DNA adjacent to either a 5' or 3' si...

journal_title：Biochemistry

authors： Morris PD,Byrd AK,Tackett AJ,Cameron CE,Tanega P,Ott R,Fanning E,Raney KD

更新日期：2002-02-19 00:00:00

abstract：:The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...

journal_title：Biochemistry

authors： Yu SS,Ji CZ,Wu YP,Lee TL,Lai CH,Lin SC,Yang ZL,Wang VC,Chen KH,Chan SI

更新日期：2007-12-04 00:00:00

abstract：:In affinity selection, calcineurin selects from a population of randomly modified calmodulins those species with which it prefers to interact. The method shows that acetylation of lysines affects calmodulin so as to interfere with its ability to interact with calcineurin. Monoacetylation of any lysine of calmodulin re...

journal_title：Biochemistry

authors： Manalan AS,Klee CB

更新日期：1987-03-10 00:00:00

abstract：:Control of protein phosphatases is now understood to depend on binding to a variety of regulatory or targeting subunits to form holoenzymes with restricted localization and substrate specificity. In addition, the catalytic subunits of both type-1 and type-2 phosphatases bind specific inhibitor proteins. Here, we repor...

journal_title：Biochemistry

authors： Eto M,Karginov A,Brautigan DL

更新日期：1999-12-21 00:00:00

abstract：:Steroid sulfatases are responsible for the hydrolysis of 3beta-hydroxy steroid sulfates, such as cholesterol and pregnenolone sulfate, and have an important role in regulating the synthesis of estrogenic steroids, from estrone sulfate and dehydroepiandrosterone sulfate, in endocrine-dependent tumors. Although little i...

journal_title：Biochemistry

authors： Purohit A,Williams GJ,Howarth NM,Potter BV,Reed MJ

更新日期：1995-09-12 00:00:00

abstract：:Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...

journal_title：Biochemistry

authors： Showalter AK,Tsai MD

更新日期：2002-08-27 00:00:00

abstract：:Dendritic cell-specific intracellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) is a C-type lectin highly expressed on the surface of antigen-presenting dendritic cells. DC-SIGN mediates interactions among dendritic cells, pathogens, and a variety of epithelia, myeloid cells, and endothelia by binding to high...

journal_title：Biochemistry

authors： Pederson K,Mitchell DA,Prestegard JH

更新日期：2014-09-09 00:00:00

abstract：:The influence of DNA base sequence context on the removal of a bulky benzo[a]pyrene diol epoxide-guanine adduct, (+)-trans-B[a]P-N2-dG (G*), by UvrABC nuclease from the thermophilic organism Bacillus caldotenax was investigated. The lesion was flanked by either T or C in otherwise identical complementary 43-mer duplex...

journal_title：Biochemistry

authors： Ruan Q,Liu T,Kolbanovskiy A,Liu Y,Ren J,Skorvaga M,Zou Y,Lader J,Malkani B,Amin S,Van Houten B,Geacintov NE

更新日期：2007-06-12 00:00:00

abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

journal_title：Biochemistry

authors： Vedeckis WV

更新日期：1983-04-12 00:00:00

abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

journal_title：Biochemistry

authors： Bjornson KP,Allen DJ,Modrich P

更新日期：2000-03-21 00:00:00

abstract：:Asymmetrically modified hemoglobins, [alpha(des-Arg)beta]A[alpha beta]cXL, [alpha(des-Arg-Tyr)beta]A[alpha beta]cXL, [alpha(des-Arg)beta(NES)]A[alpha beta]cXL, and [alpha(des-Arg)beta]A[alpha beta(NES)]cXL, have been prepared from chemically modified human normal adult hemoglobin (Hb A) and mutant hemoglobin C (beta 6...

journal_title：Biochemistry

authors： Miura S,Ho C

更新日期：1984-05-22 00:00:00

abstract：:We investigated how the intrinsic sequence-dependent properties probed via the phosphate linkages (BI ↔ BII equilibrium) influence the preferred shape of free DNA, and how this affects the nucleosome formation. First, this exploits NMR solution studies of four B-DNA dodecamers that together cover 39 base pairs of the ...

journal_title：Biochemistry

authors： Xu X,Ben Imeddourene A,Zargarian L,Foloppe N,Mauffret O,Hartmann B

更新日期：2014-09-09 00:00:00