当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Reduction of ferrylmyoglobin and ferrylhemoglobin by nitric oxide: a protective mechanism against ferryl hemoprotein-induced oxidations.

Reduction of ferrylmyoglobin and ferrylhemoglobin by nitric oxide: a protective mechanism against ferryl hemoprotein-induced oxidations.

Abstract：

:The reactions of metmyoglobin (metMb) and methemoglobin (metHb), oxidized to their respective oxoferryl free radical species (.Mb-FeIV = O/.Hb-4FeIV = O) by tert-butyl hydroperoxide (t-BuOOH), with nitric oxide (NO.) were studied by a combination of optical, electron spin resonance (ESR), ionspray mass (MS), fluorescence, and chemiluminescence spectrometries to gain insight into the mechanism by which NO. protects against oxidative injury produced by .Mb-FeIV = O/.Hb-4FeIV = O. Oxidation of metMb/metHb by t-BuOOH in a nitrogen atmosphere proceeded via the formation of two protein electrophilic centers, which were heme oxoferryl and the apoprotein radical centered at tyrosine (for the .Mb-FeIV = O form, the g value was calculated to be 2.0057), and was accompanied by the formation of t-BuOOH-derived tert-butyl(per)oxyl radicals. We hypothesized that NO. may reduce both oxoferryl and apoprotein free radical electrophilic centers of .Mb-FeIV = O/.Hb-4FeIV = O and eliminate tert-butyl(per)oxyl radicals, thus protecting against oxidative damage. We found that NO. reduced .Mb-FeIV = O/.Hb-4FeIV = O to their respective ferric (met) forms and prevented the following: (i) oxidation of cis-parinaric acid (PnA) in liposomes, (ii) oxidation of luminol, and (iii) formation of the tert-butyl(per)oxyl adduct with the spin trap DMPO. NO. eliminated the signals of tyrosyl radical detected by ESR and oxoferryl detected by MS in the reaction of t-BuOOH with metMb. As evidenced by MS of apomyoglobin, this effect was due to the two-electron reduction of .Mb-FeIV = O by NO. at the oxoferryl center rather than to nitrosylation of the tyrosine residues. Results of our in vitro experiments suggest that NO. exhibits a potent, targetable antioxidant effect against oxidative damage produced by oxoferryl Mb/Hb.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Gorbunov NV,Osipov AN,Day BW,Zayas-Rivera B,Kagan VE,Elsayed NM

doi

10.1021/bi00020a014

subject

Has Abstract

pub_date

1995-05-23 00:00:00

pages

6689-99

issue

20

eissn

0006-2960

issn

1520-4995

journal_volume

34

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Determination of the size of rat ribosomal deoxyribonucleic acid repeating units by electron microscopy.

    abstract：:The empoyment of a novel method of affinity chromatography, which makes use of antibodies that specifically bind DNA/RNA hybrids, has made it possible to enrich for rat rDNA molecules which contain R loops formed with the 18S and 28S rRNAs. An approximately 150-fold enrichment of the ratrRNA coding sequences was obtai...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00580a030

    authors： Stumph WE,Wu JR,Bonner J

    更新日期：1979-06-26 00:00:00

  • Time-resolved fluorescence studies of genetically engineered Escherichia coli glutamine synthetase. Effects of ATP on the tryptophan-57 loop.

    abstract：:Single-tryptophan-containing mutants of low adenylation state Escherichia coli glutamine synthetase (wild type has two tryptophans at positions 57 and 158) have been constructed and studied by multifrequency phase/modulation fluorescence spectroscopy. The W57L mutant (retains tryptophan at residue 158) and the W158S m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00228a008

    authors： Atkins WM,Stayton PS,Villafranca JJ

    更新日期：1991-04-09 00:00:00

  • Molecular dynamics, free energy, and SPR analyses of the interactions between the SH2 domain of Grb2 and ErbB phosphotyrosyl peptides.

    abstract：:We studied the interactions between the SH2 domain of growth factor receptor binding protein 2 (Grb2) and ErbB receptor-derived phosphotyrosyl peptides using molecular dynamics, free energy calculations, and surface plasmon resonance (SPR) analysis. Binding free energies for nine phosphotyrosyl peptides were calculate...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034113h

    authors： Suenaga A,Hatakeyama M,Ichikawa M,Yu X,Futatsugi N,Narumi T,Fukui K,Terada T,Taiji M,Shirouzu M,Yokoyama S,Konagaya A

    更新日期：2003-05-13 00:00:00

  • Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings.

    abstract：:Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050152c

    authors： Jain NU,Tjioe E,Savidor A,Boulie J

    更新日期：2005-06-28 00:00:00

  • Self-Competitive Inhibition of the Bacteriophage P22 Tailspike Endorhamnosidase by O-Antigen Oligosaccharides.

    abstract：:The P22 tailspike endorhamnosidase confers the high specificity of bacteriophage P22 for some serogroups of Salmonella differing only slightly in their O-antigen polysaccharide. We used several biophysical methods to study the binding and hydrolysis of O-antigen fragments of different lengths by P22 tailspike protein....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00872

    authors： Baxa U,Weintraub A,Seckler R

    更新日期：2020-12-29 00:00:00

  • Mechanistic Characterization of Escherichia coli l-Aspartate Oxidase from Kinetic Isotope Effects.

    abstract：:l-Aspartate oxidase, encoded by the nadB gene, is the first enzyme in the de novo synthesis of NAD+ in bacteria. This FAD-dependent enzyme catalyzes the oxidation of l-aspartate to generate iminoaspartate and reduced flavin. Distinct from most amino acid oxidases, it can use either molecular oxygen or fumarate to reox...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00307

    authors： Chow C,Hegde S,Blanchard JS

    更新日期：2017-08-08 00:00:00

  • The crystal structure of the C-terminal truncated apolipoprotein A-I sheds new light on amyloid formation by the N-terminal fragment.

    abstract：:Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi2017014

    authors： Gursky O,Mei X,Atkinson D

    更新日期：2012-01-10 00:00:00

  • Systematic evaluation of candidate ligands regulating ectodomain shedding of amyloid precursor protein.

    abstract：:Despite intense interest in the proteolysis of the β-Amyloid Precursor Protein (APP) in Alzheimer's disease, how the normal processing of this type I receptor-like glycoprotein is physiologically regulated remains ill-defined. In recent years, several candidate protein ligands for APP, including F-spondin, Reelin, β1 ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400165f

    authors： Rice HC,Young-Pearse TL,Selkoe DJ

    更新日期：2013-05-14 00:00:00

  • Role of the Cysteine 81 Residue of Macrophage Migration Inhibitory Factor as a Molecular Redox Switch.

    abstract：:Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01156

    authors： Schinagl A,Kerschbaumer RJ,Sabarth N,Douillard P,Scholz P,Voelkel D,Hollerweger JC,Goettig P,Brandstetter H,Scheiflinger F,Thiele M

    更新日期：2018-03-06 00:00:00

  • Acylation of lysine 860 allows tight binding and cytotoxicity of Bordetella adenylate cyclase on CD11b-expressing cells.

    abstract：:The Bordetella adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) forms cation-selective membrane channels and delivers into the cytosol of target cells an adenylate cyclase domain (AC) that catalyzes uncontrolled conversion of cellular ATP to cAMP. Both toxin activities were previously shown to depend on post-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050459b

    authors： Masin J,Basler M,Knapp O,El-Azami-El-Idrissi M,Maier E,Konopasek I,Benz R,Leclerc C,Sebo P

    更新日期：2005-09-27 00:00:00

  • Structure and dynamics of an acid-denatured protein G mutant.

    abstract：:NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9920230

    authors： Sari N,Alexander P,Bryan PN,Orban J

    更新日期：2000-02-08 00:00:00

  • Characterization of the tertiary structure of soluble CD4 bound to glycosylated full-length HIVgp120 by chemical modification of arginine residues and mass spectrometric analysis.

    abstract：:The initial step of infection of blood cells with the human immunodeficiency virus, HIV, is the formation of a complex of the viral envelope protein gp120 and its human receptor CD4. We have examined structural features of recombinant soluble CD4 (sCD4) by chemical modification of arginine residues with hydroxyphenylg...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011626k

    authors： Hager-Braun C,Tomer KB

    更新日期：2002-02-12 00:00:00

  • Inactivation of photoexcited rhodopsin in retinal rods: the roles of rhodopsin kinase and 48-kDa protein (arrestin).

    abstract：:The inactivation of excited rhodopsin in the presence of ATP, rhodopsin kinase, and/or arrestin has been studied from its effect on the two subsequent steps in the light-induced enzymatic cascade: metarhodopsin II catalyzed activation of G-protein and G-protein-dependent activation of cGMP phosphodiesterase. The inact...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00405a049

    authors： Bennett N,Sitaramayya A

    更新日期：1988-03-08 00:00:00

  • Phosphorescence studies of the interaction of myelin basic protein with phosphatidylserine vesicles.

    abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00514a019

    authors： Vadas EB,Melançon P,Braun PE,Galley WC

    更新日期：1981-05-26 00:00:00

  • Protected sulfur transfer reactions by the Escherichia coli Suf system.

    abstract：:The first step in sulfur mobilization for the biosynthesis of Fe-S clusters under oxidative stress and iron starvation in Escherichia coli involves a cysteine desulfurase SufS. Its catalytic reactivity is dependent on the presence of a sulfur acceptor protein, SufE, which acts as the preferred substrate for this enzym...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4001479

    authors： Selbach BP,Pradhan PK,Dos Santos PC

    更新日期：2013-06-11 00:00:00

  • Domain-specific chaperone-induced expansion is required for beta-actin folding: a comparison of beta-actin conformations upon interactions with GroEL and tail-less complex polypeptide 1 ring complex (TRiC).

    abstract：:Actin, an abundant cytosolic protein in eukaryotic cells, is dependent on the interaction with the chaperonin tail-less complex polypeptide 1 ring complex (TRiC) to fold to the native state. The prokaryotic chaperonin GroEL also binds non-native beta-actin, but is unable to guide beta-actin toward the native state. In...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700658n

    authors： Villebeck L,Moparthi SB,Lindgren M,Hammarström P,Jonsson BH

    更新日期：2007-11-06 00:00:00

  • Ultraviolet resonance Raman spectra of insulin and alpha-lactalbumin with 218- and 200-nm laser excitation.

    abstract：:Ultraviolet resonance Raman (RR) spectra, with 200- and 218-nm excitation from a H2-shifted quadrupled Nd:YAG laser, are reported for insulin and alpha-lactalbumin in dilute aqueous solution, at pH values known to produce differences in the exposure of the aromatic residues to solvent. At 200 nm, the spectra are domin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00329a009

    authors： Rava RP,Spiro TG

    更新日期：1985-04-09 00:00:00

  • Structure and mechanism of DNA polymerase β.

    abstract：:DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains. Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate lyase) during the repair of simple base lesions. These domains are termed the polymerase and lyase domains, respectively. Pol β has been an excelle...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500139h

    authors： Beard WA,Wilson SH

    更新日期：2014-05-06 00:00:00

  • Catalytically active cross-species heterodimers of thymidylate synthase.

    abstract：:Thymidylate synthase (TS) is a highly conserved homodimeric enzyme with two active sites, each of which contains amino acid residues from both subunits. We show that the conservation at the subunit interface between Escherichia coli TS and Lactobacillus casei TS is sufficient to permit the formation of a cross-species...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00090a002

    authors： Greene PJ,Maley F,Pedersen-Lane J,Santi DV

    更新日期：1993-10-05 00:00:00

  • Photooxidation of cytochrome b559 in oxygen-evolving photosystem II.

    abstract：:Cytochrome b559 (cyt b559) is an intrinsic and essential component of the photosystem II (PSII) protein complex, but its function, stoichiometry, and electron-transfer kinetics in the physiological system are not well-defined. In this study, we have used flash-detection optical spectroscopy to measure the kinetics and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00161a025

    authors： Buser CA,Diner BA,Brudvig GW

    更新日期：1992-11-24 00:00:00

  • Isoenergetic microarrays to study the structure and interactions of DsrA and OxyS RNAs in two- and three-component complexes.

    abstract：:Information on the secondary structure and interactions of RNA is important to understand the biological function of RNA as well as in applying RNA as a tool for therapeutic purposes. Recently, the isoenergetic microarray mapping method was developed to improve the prediction of RNA secondary structure. Herein, for th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200463p

    authors： Fratczak A,Kierzek R,Kierzek E

    更新日期：2011-09-06 00:00:00

  • One-disulfide intermediates of apamin exhibit native-like structure.

    abstract：:The conformations of three peptide models of the one-disulfide and fully reduced forms of apamin were characterized by using nuclear magnetic resonance (NMR) spectroscopy. Apa-2 contains the native disulfide bond between Cys3 and Cys15 in apamin with the other two cysteines replaced by alanines. Apa-1 contains the nat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00183a031

    authors： Xu X,Nelson JW

    更新日期：1994-05-03 00:00:00

  • Mechanism of bacteriophage T4 DNA holoenzyme assembly: the 44/62 protein acts as a molecular motor.

    abstract：:The role of ATP hydrolysis by the 44/62 protein in formation of the stable holoenzyme DNA replication complex has been further elucidated by specifically examining the role that the 44/62 protein plays in loading the 45 protein onto the DNA substrate. A stable phospho-45 protein or phosphorylated holoenzyme complex wa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962139l

    authors： Berdis AJ,Benkovic SJ

    更新日期：1997-03-11 00:00:00

  • Gross structural changes in isolated liver cell plasma membranes upon binding of insulin.

    abstract：:The addition of 10(-9) M insulin to a suspension of rat liver plasma membranes increases the overall lipid microviscosity, eta, by about 10--20%. The effect is confined to physiological concentrations of the hormone and is highly specific. The specificity was demonstrated in experiments where insulin analogues were ad...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00570a009

    authors： Luly P,Shinitzky M

    更新日期：1979-02-06 00:00:00

  • Cyanide binding at the non-heme Fe2+ of the iron-quinone complex of photosystem II: at high concentrations, cyanide converts the Fe2+ from high (S = 2) to low (S = 0) spin.

    abstract：:The primary electron acceptor complex of photosystem II, QAFe2+, can bind a number of small molecules at the iron site, including cyanide [Koulougliotis, D., Kostopoulos, T., Petrouleas, V., & Diner, B. A. (1993) Biochim. Biophys. Acta 1141, 275-282)]. In the presence of NaCN (30-300 mM) at pH 6.5, the reduced state, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00199a014

    authors： Sanakis Y,Petrouleas V,Diner BA

    更新日期：1994-08-23 00:00:00

  • Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.

    abstract：:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0500832

    authors： Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

    更新日期：2005-06-21 00:00:00

  • Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site.

    abstract：:Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00271a036

    authors： Dalbey RE,Wells JA,Yount RG

    更新日期：1983-01-18 00:00:00

  • Treponema denticola PurE Is a bacterial AIR carboxylase.

    abstract：:De novo purine biosynthesis proceeds by two divergent paths. In bacteria, yeasts, and plants, 5-aminoimidazole ribonucleotide (AIR) is converted to 4-carboxy-AIR (CAIR) by two enzymes: N(5)-carboxy-AIR (N(5)-CAIR) synthetase (PurK) and N(5)-CAIR mutase (class I PurE). In animals, the conversion of AIR to CAIR requires...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi102033a

    authors： Tranchimand S,Starks CM,Mathews II,Hockings SC,Kappock TJ

    更新日期：2011-05-31 00:00:00

  • The Q-loop of DrrA is involved in producing the closed conformation of the nucleotide binding domains and in transduction of conformational changes between DrrA and DrrB.

    abstract：:DrrA and DrrB proteins form an ATP-dependent efflux pump for doxorubicin and daunorubicin in Streptomyces peucetius. DrrA, the catalytic subunit, forms a complex with the integral membrane protein DrrB. Previous studies have provided evidence for strong interaction between these two proteins, which was found to be cri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701699a

    authors： Rao DK,Kaur P

    更新日期：2008-03-04 00:00:00

  • Catalytic properties of hairpin ribozymes derived from Chicory yellow mottle virus and arabis mosaic virus satellite RNAs.

    abstract：:Regions of the negative strands of the satellite RNAs of chicory yellow mottle virus (sCYMV1) and arabis mosaic virus (sArMV) have similarity in sequence and predicted secondary structure compared to the tobacco ringspot virus satellite RNA (sTRSV) hairpin ribozyme, suggesting that they may also be catalytic RNAs of a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00048a024

    authors： DeYoung M,Siwkowski AM,Lian Y,Hampel A

    更新日期：1995-12-05 00:00:00