当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.

Identification of tyrosine residues on ELMO1 that are phosphorylated by the Src-family kinase Hck.

Abstract：

:The SH3 and SH2 domains of hematopoietic cell kinase (Hck) play important roles in substrate targeting. To identify new components of Hck signaling pathways, we identified proteins that bind to the SH3 domain of Hck (Scott et al. (2002) J. Biol. Chem. 277, 28238). One such protein was ELMO1, the mammalian orthologue of the Caenorhabditis elegans gene, ced-12. ELMO1 is an approximately 80-kD protein containing a PH domain and a C-terminal Pro-rich sequence. In C. elegans, ced-12 is required for the engulfment of dying cells and for cell migration. In mammalian fibroblasts, ELMO1 binds to Dock180, and functions upstream of Rac during phagocytosis and cell migration. We previously showed that ELMO1 binds directly to the Hck SH3 domain and is phosphorylated by Hck. In this study, we used mass spectrometry to identify the following sites of ELMO1 phosphorylation: Tyr 18, Tyr 216, Tyr 511, Tyr 395, and Tyr 720. Mutant forms of ELMO1 lacking these sites were defective in their ability to promote phagocytosis and migration in fibroblasts. Single tyrosine mutations showed that Tyr 511 is particularly important in mediating these biological effects. These mutants displayed comparable binding to Dock180 and Crk as wild-type ELMO1, but gave a lowered activation of Rac. The data suggest that Src family kinase mediated tyrosine phosphorylation of ELMO1 might represent an important regulatory mechanism that controls signaling through the ELMO1/Crk/Dock180 pathway.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Yokoyama N,deBakker CD,Zappacosta F,Huddleston MJ,Annan RS,Ravichandran KS,Miller WT

doi

10.1021/bi0500832

subject

Has Abstract

pub_date

2005-06-21 00:00:00

pages

8841-9

issue

24

eissn

0006-2960

issn

1520-4995

journal_volume

44

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Quenching of chlorophyll a fluorescence in the aggregates of LHCII: steady state fluorescence and picosecond relaxation kinetics.

    abstract：:The protein composition, steady state and time-resolved fluorescence emission spectra were studied in solubilized and aggregated LHCII complexes, that were prepared according to two different isolation protocols: (1) by fractionation of cation-depleted thylakoid membranes using the non-ionic detergent Triton X-100 acc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9625253

    authors： Vasil'ev S,Irrgang KD,Schrötter T,Bergmann A,Eichler HJ,Renger G

    更新日期：1997-06-17 00:00:00

  • The Ebola Viral Protein 35 N-Terminus Is a Parallel Tetramer.

    abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01154

    authors： Chanthamontri CK,Jordan DS,Wang W,Wu C,Lin Y,Brett TJ,Gross ML,Leung DW

    更新日期：2019-02-12 00:00:00

  • The imine-pyridine torsion of the pyridoxal 5'-phosphate Schiff base of aspartate aminotransferase lowers its pKa in the unliganded enzyme and is crucial for the successive increase in the pKa during catalysis.

    abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981517e

    authors： Hayashi H,Mizuguchi H,Kagamiyama H

    更新日期：1998-10-27 00:00:00

  • Refolding of amphioxus insulin-like peptide: implications of a bifurcating evolution of the different folding behavior of insulin and insulin-like growth factor 1.

    abstract：:Insulin and insulin-like growth factor 1 (IGF-1) share high sequence homology, but their folding behaviors are significantly different: insulin folds into one unique thermodynamically controlled structure, while IGF-1 folds into two thermodynamically controlled disulfide isomers. However, the origin of their different...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0346289

    authors： Wang S,Guo ZY,Shen L,Zhang YJ,Feng YM

    更新日期：2003-08-19 00:00:00

  • Function of arginine-234 and aspartic acid-271 in domain closure, cooperativity, and catalysis in Escherichia coli aspartate transcarbamylase.

    abstract：:Two mutant versions of Escherichia coli aspartate transcarbamylase were created by site-specific mutagenesis. Arg-234 of the 240s loop was replaced by serine in order to help deduce the function of the interactions that normally occur between Arg-234 and both Glu-50 and Gln-231 in the R state of the enzyme. The other ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00423a022

    authors： Middleton SA,Kantrowitz ER

    更新日期：1988-11-15 00:00:00

  • Epsilon amino caproic acid inhibits streptokinase-plasminogen activator complex formation and substrate binding through kringle-dependent mechanisms.

    abstract：:Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992028x

    authors： Lin LF,Houng A,Reed GL

    更新日期：2000-04-25 00:00:00

  • Small-angle neutron scattering study of Bence-Jones protein Mcg: comparison of structures in solution and in crystal.

    abstract：:Immunoglobulins fragments are composed of globular domains linked by extended polypeptide segments. The molecular flexibility inherent in this arrangement allows for significant potential differences between structures observed in the crystalline state and those attained in solution. Small-angle neutron scattering mea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00541a011

    authors： Schiffer M,Stevens FJ,Westholm FA,Kim SS,Carlson RD

    更新日期：1982-06-08 00:00:00

  • Ligand-free open-closed transitions of periplasmic binding proteins: the case of glutamine-binding protein.

    abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902045p

    authors： Bermejo GA,Strub MP,Ho C,Tjandra N

    更新日期：2010-03-09 00:00:00

  • Multiple conformations of NAD and NADH when bound to human cytosolic and mitochondrial aldehyde dehydrogenase.

    abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi012197t

    authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

    更新日期：2002-06-04 00:00:00

  • Effect of temperature on the intrinsic flexibility of DNA and its interaction with architectural proteins.

    abstract：:The helical structure of double-stranded DNA is destabilized by increasing temperature. Above a critical temperature (the melting temperature), the two strands in duplex DNA become fully separated. Below this temperature, the structural effects are localized. Using tethered particle motion in a temperature-controlled ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500344j

    authors： Driessen RP,Sitters G,Laurens N,Moolenaar GF,Wuite GJ,Goosen N,Dame RT

    更新日期：2014-10-21 00:00:00

  • Pressure effects on dipalmitoylphosphatidylcholine bilayers measured by 2H nuclear magnetic resonance.

    abstract：:The effects of pressure, up to 5 kbar, on multilamellar vesicles of 1,2-dipalmitoyl-sn-phosphatidylcholine perdeuterated in the acyl chains (DPPC-d62) were examined by using high-pressure NMR techniques. A deuterium probe was built, and the quadrupole splitting was measured against pressure at various temperatures. Th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00227a022

    authors： Driscoll DA,Samarasinghe S,Adamy S,Jonas J,Jonas A

    更新日期：1991-04-02 00:00:00

  • Identification of peptide antagonists to glycoprotein Ibalpha that selectively inhibit von Willebrand factor dependent platelet aggregation.

    abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702428q

    authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

    更新日期：2008-04-22 00:00:00

  • 1H NMR studies of tris(phenanthroline) metal complexes bound to oligonucleotides: structural characterizations via selective paramagnetic relaxation.

    abstract：:The selective paramagnetic relaxation of oligonucleotide protons of d(GTGCAC)2 by delta- and lambda-Ni(phen)3(3+) and delta- and lambda-Cr(phen)3(3+) has been examined to obtain some structural insight into the noncovalent binding of tris(phenanthroline) metal complexes to DNA. The experiments demonstrate that the rel...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00459a007

    authors： Rehmann JP,Barton JK

    更新日期：1990-02-20 00:00:00

  • Excitation energy transfer studies on the proximity between SH1 and the adenosinetriphosphatase site in myosin subfragment 1.

    abstract：:Excitation energy transfer studies were carried out to determine the distance between the adenosinetriphosphatase (ATPase) site and a unique "fast-reacting" sulfhydryl (referred to as SH1) in myosin subfragment 1. The fluorescent moiety of the probe N-(iodoacetyl)-N'-(5-sulfo-1-naphthyl)ethylene-diamine was used as th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00520a035

    authors： Tao T,Lamkin M

    更新日期：1981-08-18 00:00:00

  • C5 cytosine methylation at CpG sites enhances sequence selectivity of mitomycin C-DNA bonding.

    abstract：:We have established that UvrABC nuclease is equally efficient in cutting mitomycin C (MC)-DNA monoadducts formed at different sequences and that the degree of UvrABC cutting represents the extent of drug-DNA bonding. Using this method we determined the effect of C5 cytosine methylation on the DNA monoalkylation by MC ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991307h

    authors： Li VS,Reed M,Zheng Y,Kohn H,Tang M

    更新日期：2000-03-14 00:00:00

  • Binding specificity of multiprotein signaling complexes is determined by both cooperative interactions and affinity preferences.

    abstract：:The generation of multiprotein complexes at receptors and adapter proteins is crucial for the activation of intracellular signaling pathways. In this study, we used multiple biochemical and biophysical methods to examine the binding properties of several SH2 and SH3 domain-containing signaling proteins as they interac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0357311

    authors： Houtman JC,Higashimoto Y,Dimasi N,Cho S,Yamaguchi H,Bowden B,Regan C,Malchiodi EL,Mariuzza R,Schuck P,Appella E,Samelson LE

    更新日期：2004-04-13 00:00:00

  • Structure of an active soluble mutant of the membrane-associated (S)-mandelate dehydrogenase.

    abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010938k

    authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

    更新日期：2001-08-21 00:00:00

  • RuvB protein-mediated ATP hydrolysis: functional asymmetry in the RuvB hexamer.

    abstract：:A survey of RuvB protein-mediated ATP hydrolysis yields the following observations. (1) The RuvB protein exhibits a DNA-independent ATPase activity with a turnover number (based on a RuvB monomer) approaching 6 min-1 and a Km of 154 microM. Single-stranded DNA and linear duplex DNA have small but significant effects o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00030a019

    authors： Marrione PE,Cox MM

    更新日期：1995-08-01 00:00:00

  • Mechanistic studies of two dioxygenases in the methionine salvage pathway of Klebsiella pneumoniae.

    abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010110y

    authors： Dai Y,Pochapsky TC,Abeles RH

    更新日期：2001-05-29 00:00:00

  • Ferrate oxidation of murine leukemia virus reverse transcriptase: identification of the template-primer binding domain.

    abstract：:Treatment of murine leukemia virus reverse transcriptase (MuLV RT) with potassium ferrate, an oxidizing agent known to oxidize amino acids involved in phosphate binding domains of proteins, results in the irreversible inactivation of both the DNA polymerase and the RNase H activities. Significant protection from ferra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00247a015

    authors： Reddy G,Nanduri VB,Basu A,Modak MJ

    更新日期：1991-08-20 00:00:00

  • Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.

    abstract：:We have determined the fidelity of in vitro DNA synthesis catalyzed at high temperature by the DNA polymerase from the thermophilic bacterium Thermus aquaticus. Using a DNA substrate that contains a 3'-OH terminal mismatch, we demonstrate that the purified polymerase lacks detectable exonucleolytic proofreading activi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00416a027

    authors： Tindall KR,Kunkel TA

    更新日期：1988-08-09 00:00:00

  • Molecular hinges in protein folding: the urea-denatured state of apomyoglobin.

    abstract：:Unfolded apomyoglobin in 8 M urea at pH 2.3 displays distinct regions with different backbone mobility, as monitored by NMR relaxation. These variations in backbone mobility can be correlated with intrinsic properties of the amino acids in the sequence. Clusters of small amino acids such as glycine and alanine show in...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi020381o

    authors： Schwarzinger S,Wright PE,Dyson HJ

    更新日期：2002-10-22 00:00:00

  • Formation and rejoining of deoxyribonucleic acid double-strand breaks induced in isolated cell nuclei by antineoplastic intercalating agents.

    abstract：:The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a013

    authors： Pommier Y,Schwartz RE,Kohn KW,Zwelling LA

    更新日期：1984-07-03 00:00:00

  • Calorimetric studies of adenosine 5'-triphosphate hydrolysis by heavy meromyosin.

    abstract：:The heat production for the reaction steps of ATP hydrolysis by heavy meromyosin has been reexamined by using a reaction-type calorimeter. So far, the values reported for the heat production have varied substantially among research groups. The present results obtained in 0.3 M KCl, 10 mM MgCl2, and 20 mM Tris-HCl at p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00518a037

    authors： Yamada T,Shimizu H,Suga H

    更新日期：1981-07-21 00:00:00

  • Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy.

    abstract：:Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The corresp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00504a600

    authors： Johnson FA,Lewis SD,Shafer JA

    更新日期：1981-01-06 00:00:00

  • Fourier transform Raman spectroscopy of the bacteriorhodopsin mutant Tyr-185-->Phe: formation of a stable O-like species during light adaptation and detection of its transient N-like photoproduct.

    abstract：:Near-infrared FT-Raman spectroscopy can be used to measure the vibrations of the bacteriorhodopsin (bR) chromophore without the disadvantage of conventional visible resonance Raman spectroscopy, where the visible excitation drives the bR photoreactions. We utilized this technique to investigate the light-dark adaptati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00060a020

    authors： Rath P,Krebs MP,He Y,Khorana HG,Rothschild KJ

    更新日期：1993-03-09 00:00:00

  • New class of bacterial membrane oxidoreductases.

    abstract：:A new class of bacterial multisubunit membrane-bound electron-transfer complexes has been identified based on biochemical and bioinformatic data. It contains subunits homologous to the three-subunit molybdopterin oxidoreductases and four additional subunits, two of which are c-type cytochromes. The complex was purifie...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047267l

    authors： Yanyushin MF,del Rosario MC,Brune DC,Blankenship RE

    更新日期：2005-08-02 00:00:00

  • Structure of Ralstonia eutropha flavohemoglobin in complex with three antibiotic azole compounds.

    abstract：:Flavohemoglobins (flavoHbs) are enzymes that operate primarily as nitric oxide dioxygenases and shuttle thereby electrons among NAD(P)H, FAD, heme, and a ligated redox-active substrate such as O(2). They function in the bacterial defense against nitrosative stress and are therefore considered as targets for new antibi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101650q

    authors： El Hammi E,Warkentin E,Demmer U,Limam F,Marzouki NM,Ermler U,Baciou L

    更新日期：2011-02-22 00:00:00

  • Simple purification and functional reconstitution of octopus photoreceptor Gq, which couples rhodopsin to phospholipase C.

    abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961360v

    authors： Kikkawa S,Tominaga K,Nakagawa M,Iwasa T,Tsuda M

    更新日期：1996-12-10 00:00:00

  • Applicability of the induced-fit model to glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. Study of the binding of oxidized nicotinamide adenine dinucleotide and nicotinamide 8-bromoadenine dinucleotide.

    abstract：:A new method of calculation, based on a direct fitting of the protein fluorescence intensity observed upon coenzyme binding (H.-P. Lutz, unpublished results), is used to study the negative cooperative behavior of glyceraldehyde-3-phosphate dehydrogenase from sturgeon muscle. The calculation procedure simultaneously el...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00288a014

    authors： Branlant G,Eiler B,Biellmann JF,Lutz HP,Luisi PL

    更新日期：1983-09-13 00:00:00