Binding specificity of multiprotein signaling complexes is determined by both cooperative interactions and affinity preferences.

abstract：:Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...

journal_title：Biochemistry

authors： Ohanian V,Wolfe LC,John KM,Pinder JC,Lux SE,Gratzer WB

更新日期：1984-09-11 00:00:00

abstract：:Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...

journal_title：Biochemistry

authors： Watanabe S,Wakasugi K

更新日期：2008-05-13 00:00:00

abstract：:In human glutathione transferase (GST) A1-1, the C-terminal region covers the active site and contributes to substrate binding. This region is flexible, but upon binding of an active-site ligand, it is stabilized as an amphipatic alpha-helix. The stabilization has implications for the catalytic activity of the enzyme....

journal_title：Biochemistry

authors： Gustafsson A,Etahadieh M,Jemth P,Mannervik B

更新日期：1999-12-07 00:00:00

abstract：:Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient using CFPS, the correct cotranslational folding of membrane proteins during CFPS is still a challenge. In this contribution, we describe a two-step protocol in w...

journal_title：Biochemistry

authors： Focke PJ,Hein C,Hoffmann B,Matulef K,Bernhard F,Dötsch V,Valiyaveetil FI

更新日期：2016-08-02 00:00:00

abstract：:The substrate specificity of the beta-glucosidase (CelB) from the hyperthermophilic archaeon Pyrococcus furiosus, a family 1 glycosyl hydrolase, has been studied at a molecular level. Following crystallization and X-ray diffraction of this enzyme, a 3.3 A resolution structural model has been obtained by molecular repl...

journal_title：Biochemistry

authors： Kaper T,Lebbink JH,Pouwels J,Kopp J,Schulz GE,van der Oost J,de Vos WM

更新日期：2000-05-02 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a dimeric protein which induces formation of new blood vessels (angiogenesis) through binding to VEGF-receptor-2 tyrosine kinase (VEGFR2 TK) or KDR (kinase insert domain-containing receptor) on the surface of endothelial cells. Angiogenesis has been shown to be essential fo...

journal_title：Biochemistry

authors： Parast CV,Mroczkowski B,Pinko C,Misialek S,Khambatta G,Appelt K

更新日期：1998-11-24 00:00:00

abstract：:The thermodynamics and kinetics of the subtransition L epsilon----P beta of sonicated unilamellar vesicles of 1-myristoyl-2-stearoylphosphatidylcholine (1M-2S-PC) and of 1-stearoyl-2-myristoylphosphatidylcholine (1S-2M-PC) were studied by equilibrium cooling curves and temperature-jump relaxation spectrometry with an ...

journal_title：Biochemistry

authors： Tümmler B,Herrmann U,Maass G,Eibl H

更新日期：1984-08-28 00:00:00

abstract：:Glycinamide ribonucleotide transformylase, the first of the two formyl group transferases of de novo purine biosynthesis requiring 10-formyltetrahydrofolate, has been purified 1500-fold, nearly to homogeneity, from the murine lymphoma cell line L5178Y. Purification of the enzyme was facilitated by the use of a gelatin...

journal_title：Biochemistry

authors： Caperelli CA

更新日期：1985-03-12 00:00:00

abstract：:A series of sulfonate esters that are attached to a noncationic minor-groove-binding N-methylpyrrole dipeptide (Lex) related to netrospin have been synthesized. The compounds prepared differ in two respects: (1) the length [(CH2)2 vs (CH2)8] of the tether between the DNA affinity binding portion of the molecule and th...

journal_title：Biochemistry

authors： Zhang Y,Chen FX,Mehta P,Gold B

更新日期：1993-08-10 00:00:00

abstract：:Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...

journal_title：Biochemistry

authors： Sali DL,Ingram R,Wendel M,Gupta D,McNemar C,Tsarbopoulos A,Chen JW,Hong Z,Chase R,Risano C,Zhang R,Yao N,Kwong AD,Ramanathan L,Le HV,Weber PC

更新日期：1998-03-10 00:00:00

abstract：:A novel liposomal method permits studies of Ca movements across the bilayers of multilamellar vesicles (MLV) which had entrapped the Ca-dependent, fluorescent indicator dye Fura 2. Ionomycin-mediated Ca translocation across MLV of phosphatidylcholine (PC)/dicetyl phosphate (DCP), 9:1, obeyed simple first-order kinetic...

journal_title：Biochemistry

authors： Blau L,Weissmann G

更新日期：1988-07-26 00:00:00

abstract：:We report differences in the interaction of two structurally similar phenanthroline intercalators, ethidium and propidium, with poly(dA).poly(dT) and poly[d(A-T)] as a function of ionic strength based on titration microcalorimetry, fluorescence titration, and hydrostatic pressure measurements. Both ethidium and propid...

journal_title：Biochemistry

authors： Marky LA,Macgregor RB Jr

更新日期：1990-05-22 00:00:00

abstract：:2H NMR spectroscopy was used to consider correspondence between existing single-crystal X-ray data for glycosphingolipids and their ceramide backbone conformation in fluid phospholipid membranes. A monoglycosylated sphingolipid, glucosylceramide (GlcCer), which represents the core structure of many important glycosphi...

journal_title：Biochemistry

authors： Hamilton KS,Jarrell HC,Brière KM,Grant CW

更新日期：1993-04-20 00:00:00

abstract：:JZTX-XI is a peptide toxin isolated from the venom of the Chinese spider Chilobrachys jingzhao. It contains 34 residues including six cysteine residues with disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Using 3'- and 5'-RACE methods, the full-length cDNA was identified as encoding an 86-residue pr...

journal_title：Biochemistry

authors： Liao Z,Yuan C,Deng M,Li J,Chen J,Yang Y,Hu W,Liang S

更新日期：2006-12-26 00:00:00

abstract：:The concept of using a dynamic base-pairing nucleobase as a mode for degenerate recognition presents a unique challenge to analysis of DNA structure. Proton and phosphorus NMR studies are reported for two nine-residue DNA oligodeoxyribonucleotides, d(CATGGGTAC).d(GTACNCATG) (1) and d(CATGTGTAC).(GTACNCATG) (2), which ...

journal_title：Biochemistry

authors： Klewer DA,Zhang P,Bergstrom DE,Davisson VJ,LiWang AC

更新日期：2001-02-13 00:00:00

abstract：:The cation complexation equilibria between ionophore A23187 and several alkaline earth and first transition series divalent cations have been investigated. Absorption and fluorescence spectroscopy were used to monitor the reactions which were studied in solutions of 80% methanol/water, at 25 degrees C, and under condi...

journal_title：Biochemistry

authors： Chapman CJ,Puri AK,Taylor RW,Pfeiffer DR

更新日期：1987-08-11 00:00:00

abstract：:The thermotropic behavior of aqueous dispersions of palmitoylsphingomyelin-cholesterol and lignoceryl-sphingomyelin-cholesterol mixtures has been examined by high-sensitivity differential scanning calorimetry. When less than 25 mol % cholesterol is mixed with either sphingomyelin, the calorimetric endotherm is compose...

journal_title：Biochemistry

authors： Estep TN,Mountcastle DB,Barenholz Y,Biltonen RL,Thompson TE

更新日期：1979-05-15 00:00:00

abstract：:Resonance Raman spectra of the photosynthetic reaction center isolated from the green bacterium Chloroflexus aurantiacus have been obtained with excitation in the near-infrared absorption bands of the special pair (P) and the accessory bacteriochlorophyll (B) using shifted-excitation Raman difference spectroscopy (SER...

journal_title：Biochemistry

authors： Cherepy NJ,Holzwarth AR,Mathies RA

更新日期：1995-04-18 00:00:00

abstract：:Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...

journal_title：Biochemistry

authors： Tan SK,Fong KP,Polizzi NF,Sternisha A,Slusky JSG,Yoon K,DeGrado WF,Bennett JS

更新日期：2019-07-30 00:00:00

abstract：:The role of bacterial DnaJ protein as a cochaperone of DnaK is strongly appreciated. Although DnaJ unaccompanied by DnaK can bind unfolded as well as native substrate proteins, its role as an individual chaperone remains elusive. In this study, we demonstrate that DnaJ binds a model non-native substrate with a low nan...

journal_title：Biochemistry

authors： Tiwari S,Kumar V,Jayaraj GG,Maiti S,Mapa K

更新日期：2013-02-12 00:00:00

abstract：:Several experimental approaches have been employed to independently determine rates of dissociation for Escherichia coli RNA polymerase from the T7 bacteriophage A1, A2, A3, and D promoters. Heparin challenge measurements employing abortive initiation turnover rates as an index of promoter occupancy were carried out o...

journal_title：Biochemistry

authors： Cech CL,McClure WR

更新日期：1980-05-27 00:00:00

abstract：:Magic angle sample spinning (MASS) 13C NMR spectra have been obtained of bovine rhodopsin regenerated with retinal prosthetic groups isotopically enriched with 13C at C-5 and C-14. In order to observe the 13C retinal chromophore resonances, it was necessary to employ low temperatures (-15-----35 degrees C) to restrict...

journal_title：Biochemistry

authors： Smith SO,Palings I,Copié V,Raleigh DP,Courtin J,Pardoen JA,Lugtenburg J,Mathies RA,Griffin RG

更新日期：1987-03-24 00:00:00

abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....

journal_title：Biochemistry

authors： Kim S,Merrill BM,Rajpurohit R,Kumar A,Stone KL,Papov VV,Schneiders JM,Szer W,Wilson SH,Paik WK,Williams KR

更新日期：1997-04-29 00:00:00

abstract：:The Xenopus borealis somatic 5S ribosomal RNA gene was used as a model system to determine the mutual effects of nucleosome folding and formation of ultraviolet (UV) photoproducts (primarily cis-syn cyclobutane pyrimidine dimers, or CPDs) in chromatin. We analyzed the preferred rotational and translational settings of...

journal_title：Biochemistry

authors： Liu X,Mann DB,Suquet C,Springer DL,Smerdon MJ

更新日期：2000-01-25 00:00:00

abstract：:Processing bodies (P-bodies) are cytoplasmic ribonucleoprotein (RNP) granules primarily composed of translationally repressed mRNAs and proteins related to mRNA decay, suggesting roles in post-transcriptional regulation. P-bodies are conserved in eukaryotic cells and exhibit properties of liquid droplets. However, the...

journal_title：Biochemistry

authors： Luo Y,Na Z,Slavoff SA

更新日期：2018-05-01 00:00:00

abstract：:In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...

journal_title：Biochemistry

authors： Bourdon P,Fenton JW 2nd,Maraganore JM

更新日期：1990-07-10 00:00:00

abstract：:The light-driven enzyme NADPH:protochlorophyllide oxidoreductase (POR) catalyzes the reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), a key regulatory step in the chlorophyll biosynthesis pathway. As POR is light activated, it allows intermediates in the reaction pathway to be observed by initiat...

journal_title：Biochemistry

authors： Heyes DJ,Ruban AV,Hunter CN

更新日期：2003-01-21 00:00:00

abstract：:Dynemicin A (1), a member of the enediyne family of natural products, binds to double-stranded DNA (K(B) approximately 10(4) M(-1)) and in the presence of millimolar concentrations of a reducing cofactor such as NADPH or GSH reacts to cleave DNA. In this work, we show that the two flavin-based enzymes ferredoxin-NADP+...

journal_title：Biochemistry

authors： Myers AG,Kort ME,Cohen SB,Tom NJ

更新日期：1997-04-01 00:00:00

abstract：:We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...

journal_title：Biochemistry

authors： Nienhaus K,Dominici P,Astegno A,Abbruzzetti S,Viappiani C,Nienhaus GU

更新日期：2010-09-07 00:00:00

abstract：:Chemical modification of some trifunctional amino acid residues in toxins I, II, and III of the scorpion Androctonus australis Hector have been performed. The results indicate: (1) Reduction and methylation of one disulfide bridge destroy toxic activity of toxin II. (2) The only tryptophan residue of toxin II (positio...

journal_title：Biochemistry

authors： Habersetzer-Rochat C,Sampieri F

更新日期：1976-06-01 00:00:00