Kinetics of the subtransition of asymmetrically substituted phosphatidylcholines.

abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

journal_title：Biochemistry

authors： Kuhlmann J,Macara I,Wittinghofer A

更新日期：1997-10-07 00:00:00

abstract：:The Ogg1 protein of Saccharomyces cerevisiae belongs to a family of DNA glycosylases and apurinic/apyrimidinic site (AP) lyases, the signature of which is the alpha-helix-hairpin-alpha-helix-Gly/Pro-Asp (HhH-GPD) active site motif together with a conserved catalytic lysine residue, to which we refer as the HhH-GPD/K f...

journal_title：Biochemistry

authors： Guibourt N,Castaing B,Van Der Kemp PA,Boiteux S

更新日期：2000-02-22 00:00:00

abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...

journal_title：Biochemistry

authors： Chartrand P,Usman N,Cedergren R

更新日期：1997-03-18 00:00:00

abstract：:The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...

journal_title：Biochemistry

authors： Rutherford TJ,Neville DC,Homans SW

更新日期：1995-10-31 00:00:00

abstract：:Myeloperoxidase plays a fundamental role in oxidant production by neutrophils. The enzyme uses hydrogen peroxide to oxidize chloride (Cl-), bromide (Br-), iodide (I-), and the pseudohalide thiocyanate (SCN-) to their respective hypohalous acids. This study for the first time presents transient kinetic measurements of ...

journal_title：Biochemistry

authors： Furtmüller PG,Burner U,Obinger C

更新日期：1998-12-22 00:00:00

abstract：:Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...

journal_title：Biochemistry

authors： Wang H,Shu Q,Frieden C,Gross ML

更新日期：2017-06-13 00:00:00

abstract：:Ultraviolet thermal denaturation studies substantiate our earlier hypothesis that substitution of a L-nucleotide residue for a D-nucleotide within a DNA duplex permits a stable structure in which all bases are paired through Watson-Crick hydrogen bonds (Damha, M. J., Giannaris, P. A., Marfey, P., & Reid, L. S. (1991) ...

journal_title：Biochemistry

authors： Damha MJ,Giannaris PA,Marfey P

更新日期：1994-06-28 00:00:00

abstract：:The electron spin resonance probes 5-doxylstearate and 4-(dodecyldimethylammonio)-1-oxy-2,2,6,6-tetramethylpiperidine bromide were used to characterize the fluidity of the acyl chain and head-group regions, respectively, of defined salts of lipopolysaccharide (LPS) from Escherichia coli K12. The removal of the weakly ...

journal_title：Biochemistry

authors： Coughlin RT,Haug A,McGroarty EJ

更新日期：1983-04-12 00:00:00

abstract：:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...

journal_title：Biochemistry

authors： Houbiers MC,Spruijt RB,Wolfs CJ,Hemminga MA

更新日期：1999-01-19 00:00:00

abstract：:A series of antisense oligonucleotides (ASOs) were synthesized and tested to define the best target sites within an RNA transcript of collagen for effective inhibition of expression. The test system consisted of mouse NIH 3T3 fibroblasts that were stably transfected with a human minigene for procollagen I so that the ...

journal_title：Biochemistry

authors： Laptev AV,Lu Z,Colige A,Prockop DJ

更新日期：1994-09-13 00:00:00

abstract：:Comparisons of catalytic mechanisms have not previously been performed for homologous enzymes from hyperthermophilic and mesophilic sources. Here, the beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical pr...

journal_title：Biochemistry

authors： Bauer MW,Kelly RM

更新日期：1998-12-08 00:00:00

abstract：:Over 155 mutations within the V2 vasopressin receptor (AVPR2) gene are responsible for nephrogenic diabetes insipidus (NDI). The expression and subcellular distribution of four of these was investigated in transfected cells. These include a point mutation in the seventh transmembrane domain (S315R), a frameshift mutat...

journal_title：Biochemistry

authors： Morello JP,Salahpour A,Petäjä-Repo UE,Laperrière A,Lonergan M,Arthus MF,Nabi IR,Bichet DG,Bouvier M

更新日期：2001-06-12 00:00:00

abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

journal_title：Biochemistry

authors： Saha R,Bose M,Sen Santara S,Roy J,Adak S

更新日期：2013-12-10 00:00:00

abstract：:The crystal structure of the complex between the quinoprotein methylamine dehydrogenase (MADH) and the type I blue copper protein amicyanin, both from Paracoccus denitrificans, has been determined at 2.5-A resolution using molecular replacement. The search model was MADH from Thiobacillus versutus. The amicyanin could...

journal_title：Biochemistry

authors： Chen L,Durley R,Poliks BJ,Hamada K,Chen Z,Mathews FS,Davidson VL,Satow Y,Huizinga E,Vellieux FM

更新日期：1992-06-02 00:00:00

abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

journal_title：Biochemistry

authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

更新日期：2006-09-05 00:00:00

abstract：:Purine C-8 tritium-labeling rates have been measured at specific sites in Escherichia coli tRNAIle and tRNA2Tyr. The results are compared with those obtained for yeast tRNAPhe (preceding paper(Gamble et al., 1976)). The tRNAIle and tRNAPhe fall into the same general class of tRNA structures, while tRNA2Tyr is in a dif...

journal_title：Biochemistry

authors： Schoemaker HJ,Gamble RC

更新日期：1976-06-29 00:00:00

abstract：:The ligation state of hemoglobin during its cross-linking by glycolaldehyde influences the ultimate oxygen affinity of the cross-linked protein. Thus, if the cross-linking is performed with carbonmonoxy-hemoglobin, the oxygen affinity increases slightly to a P50 of 7 mmHg from a P50 of 9 mmHg for unmodified hemoglobin...

journal_title：Biochemistry

authors： Manning LR,Manning JM

更新日期：1988-08-23 00:00:00

abstract：:Biogenesis of pyrroloquinoline quinone (PQQ) in Klebsiella pneumoniae requires the expression of six genes (pqqA-F). One of these genes (pqqE) encodes a 43 kDa protein (PqqE) that plays a role in the initial steps in PQQ formation [Veletrop, J. S., et al. (1995) J. Bacteriol. 177, 5088-5098]. PqqE contains two highly ...

journal_title：Biochemistry

authors： Wecksler SR,Stoll S,Tran H,Magnusson OT,Wu SP,King D,Britt RD,Klinman JP

更新日期：2009-10-27 00:00:00

abstract：:The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...

journal_title：Biochemistry

authors： Noble RW,DeYoung A,Rousseau DL

更新日期：1989-06-13 00:00:00

abstract：:Pyruvate formate-lyase activating enzyme (PFL-AE) catalyzes the generation of a catalytically essential glycyl radical on pyruvate formate-lyase (PFL). Purified PFL-AE contains an oxygen-sensitive, labile [4Fe-4S] cluster that undergoes cluster interconversions in vitro, with only the [4Fe-4S](+) cluster state being c...

journal_title：Biochemistry

authors： Yang J,Naik SG,Ortillo DO,García-Serres R,Li M,Broderick WE,Huynh BH,Broderick JB

更新日期：2009-10-06 00:00:00

abstract：:(+)-Delta-cadinene synthase (DCS) from Gossypium arboreum (tree cotton) is a sesquiterpene cyclase that catalyzes the cyclization of farnesyl diphosphate in the first committed step of the biosynthesis of gossypol, a phytoalexin that defends the plant from bacterial and fungal pathogens. Here, we report the X-ray crys...

journal_title：Biochemistry

authors： Gennadios HA,Gonzalez V,Di Costanzo L,Li A,Yu F,Miller DJ,Allemann RK,Christianson DW

更新日期：2009-07-07 00:00:00

abstract：:Calmodulin (CaM) is composed of two EF-hand domains tethered by a flexible linker. Upon Ca2+-binding, a fragment of CaM encompassing EF-hands 2 and 3 (CaM2/3; residues 46-113) folds into a structure remarkably similar to the N- and C-domains of CaM. In this study, we demonstrate that Ca2+-ligated CaM2/3 can also bind ...

journal_title：Biochemistry

authors： Lakowski TM,Lee GM,Lelj-Garolla B,Okon M,Reid RE,McIntosh LP

更新日期：2007-07-24 00:00:00

abstract：:Single-strand-specific nucleases are a diverse and important group of enzymes that are able to cleave a variety of DNA structures present in duplex molecules. Nuclease SP, an enzyme from spinach, has been purified to apparent homogeneity, allowing for the unambiguous characterization of a number of its physical proper...

journal_title：Biochemistry

authors： Strickland JA,Marzilli LG,Puckett JM Jr,Doetsch PW

更新日期：1991-10-08 00:00:00

abstract：:Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation ...

journal_title：Biochemistry

authors： Kitts CC,Beke-Somfai T,Nordén B

更新日期：2011-05-03 00:00:00

abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

journal_title：Biochemistry

authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

更新日期：2000-05-30 00:00:00

abstract：:The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phen...

journal_title：Biochemistry

authors： Ho WW,Li H,Nishida CR,de Montellano PR,Poulos TL

更新日期：2008-02-19 00:00:00

abstract：:Arginine 54 in subunit I of cytochrome c oxidase from Paracoccus denitrificans interacts with the formyl group of heme a. Mutation of this arginine to methionine (R54M) dramatically changes the spectral properties of heme a and lowers its midpoint redox potential [Kannt et al. (1999) J. Biol. Chem. 274, 37974-37981; L...

journal_title：Biochemistry

authors： Jasaitis A,Backgren C,Morgan JE,Puustinen A,Verkhovsky MI,Wikström M

更新日期：2001-05-01 00:00:00

abstract：:We report two small molecule azobenzenesulfonamide probes, CAP1 and CAP2, capable of photomodulating the activity of carbonic anhydrase (CA) on demand. In the trans form, CAP azobenzene probes adopt a linear shape, making them suitable for occupying the CA active site and interacting with Zn2+, thereby inhibiting enzy...

journal_title：Biochemistry

authors： Aggarwal K,Banik M,Medellin B,Que EL

更新日期：2019-01-08 00:00:00

abstract：:The X-ray crystal structure of a salicylhydroxamic acid (SHA) inhibitory complex with human myeloperoxidase (MPO) has been determined at 2.3 A resolution. The aromatic ring of the inhibitor binds to a hydrophobic region at the entrance to the distal heme pocket between heme pyrrole ring D and the side chain of Arg 239...

journal_title：Biochemistry

authors： Davey CA,Fenna RE

更新日期：1996-08-20 00:00:00

abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...

journal_title：Biochemistry

authors： Kaur AP,Wilks A

更新日期：2007-03-20 00:00:00