Transmembrane calcium movements mediated by ionomycin and phosphatidate in liposomes with Fura 2 entrapped.

abstract：:We have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with [3H]tamoxifen aziridine [(...

journal_title：Biochemistry

authors： Katzenellenbogen BS,Elliston JF,Monsma FJ Jr,Springer PA,Ziegler YS,Greene GL

更新日期：1987-04-21 00:00:00

abstract：:The ZFY protein is a member of one of the most interesting classes of polydactyl zinc finger proteins. It has a domain of 13 tandem zinc fingers that is organized with an internal repeat of odd-even finger pairs. It has been proposed that each finger pair interacts with six base pairs within a turn of the double helix...

journal_title：Biochemistry

authors： Grants J,Flanagan E,Yee A,Romaniuk PJ

更新日期：2010-02-02 00:00:00

abstract：:To determine whether ligand-receptor interactions could engender the selective uptake by deficient cells of enzyme-laden liposomes, aggregated human IgG was used to coat liposomes which had previously trapped purified hexosaminidase A (Hex A). By a new, high-yield procedure, Hex A was purified 7000-fold from human pla...

journal_title：Biochemistry

authors： Cohen CM,Weissmann G,Hoffstein S,Awasthi YC,Srivastava SK

更新日期：1976-01-27 00:00:00

abstract：:The voltage-sensitive sodium channel is an intrinsic membrane protein that is nonrandomly distributed in neurons, suggesting a possible interaction with other cellular constituents. In this study, we have directly tested the hypothesis that components of the cytoskeleton interact with sodium channels. Utilizing the me...

journal_title：Biochemistry

authors： Edelstein NG,Catterall WA,Moon RT

更新日期：1988-03-22 00:00:00

abstract：:Mutations in the gene encoding human ATP:cobalamin adenosyltransferase (hATR) can result in the metabolic disorder known as methylmalonic aciduria (MMA). This enzyme catalyzes the final step in the conversion of cyanocobalamin (vitamin B12) to the essential human cofactor adenosylcobalamin. Here we present the 2.5 A c...

journal_title：Biochemistry

authors： Schubert HL,Hill CP

更新日期：2006-12-26 00:00:00

abstract：:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated...

journal_title：Biochemistry

authors： Viard M,Ablan SD,Zhou M,Veenstra TD,Freed EO,Raviv Y,Blumenthal R

更新日期：2008-02-19 00:00:00

abstract：:The quaternary structure stability of proteins is typically studied under conditions that accelerate their aggregation/unfolding processes on convenient laboratory time scales. Such conditions include high temperature or pressure, chaotrope-mediated unfolding, or low or high pH. These approaches have the limitation of...

journal_title：Biochemistry

authors： Robinson LZ,Reixach N

更新日期：2014-10-21 00:00:00

abstract：:The catalytic and structural properties of divalent metal ion cofactor binding sites in the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae were investigated. Co(II)-substituted DapE enzyme was 25% more active than the Zn(II)-loaded form of the enzyme. Interestingly, Mn...

journal_title：Biochemistry

authors： Bienvenue DL,Gilner DM,Davis RS,Bennett B,Holz RC

更新日期：2003-09-16 00:00:00

abstract：:The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...

journal_title：Biochemistry

authors： Wiese A,Brandenburg K,Carroll SF,Rietschel ET,Seydel U

更新日期：1997-08-19 00:00:00

abstract：:The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...

journal_title：Biochemistry

authors： Greenspoon N,Hershkoviz R,Alon R,Varon D,Shenkman B,Marx G,Federman S,Kapustina G,Lider O

更新日期：1993-02-02 00:00:00

abstract：:Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A were assembled and compared regarding expression, secretion, and purification of the peptide hormone. After IgG affinity purification of the fusion proteins from...

journal_title：Biochemistry

authors： Moks T,Abrahmsén L,Holmgren E,Bilich M,Olsson A,Uhlén M,Pohl G,Sterky C,Hultberg H,Josephson S

更新日期：1987-08-25 00:00:00

abstract：:Leucine aminopeptidase (LAP) is a protease requiring two divalent metal cations per subunit for activity. Zn2+, Mg2+, and Co2+ metal-substituted forms of LAP have been prepared and investigated kinetically. Substitution of metal into the two binding sites independently resulted in the preparation of Zn2+Zn2+, Mg2+Zn2+...

journal_title：Biochemistry

authors： Allen MP,Yamada AH,Carpenter FH

更新日期：1983-08-02 00:00:00

abstract：:1H NMR studies on the nonselective endothelin receptor agonist sarafotoxin SRTb have identified a helix between residues Asp 8 and His 16, and a beta-turn involving residues Cys 3 to Met 6; however, the biologically important C-terminal five residues were found to be conformationally variable. The average RMSD, measur...

journal_title：Biochemistry

authors： Atkins AR,Martin RC,Smith R

更新日期：1995-02-14 00:00:00

abstract：:Accumulation of the cytotoxic 40- to 42-residue beta-amyloid peptide represents the primary pathological process in Alzheimer's disease (AD). BACE1 (beta-site APP cleaving enzyme 1) is responsible for the initial required step in the neuronal amyloidogenic processing of beta-amyloid precursor protein and is a major dr...

journal_title：Biochemistry

authors： Wang YS,Beyer BM,Senior MM,Wyss DF

更新日期：2005-12-20 00:00:00

abstract：:The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...

journal_title：Biochemistry

authors： Khan K,Madhavan TP,Kshirsagar R,Boosi KN,Sadhale P,Muniyappa K

更新日期：2013-08-06 00:00:00

abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...

journal_title：Biochemistry

authors： Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

更新日期：2004-05-11 00:00:00

abstract：:A method is described for using very high specific activity [3H]poly(deoxythymidylate) [[3H]poly(dT)] to detect, size, and quantiate subnanogram amounts of nonradioactive polyadenylated RNA. Short (approximately 100 nucleotides long) [3H]poly(dT) is hybridized to the poly(adenylate) [poly(A)] tracts in polyadenylated ...

journal_title：Biochemistry

authors： Tracy S,Kohne DE

更新日期：1980-08-05 00:00:00

abstract：:NLRP4 is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family of cytosolic receptors and a member of an inflammation signaling cascade. Here, we present the crystal structure of the NLRP4 pyrin domain (PYD) at 2.3 Å resolution. The NLRP4 PYD is a member of the death domain (DD) superfamily ...

journal_title：Biochemistry

authors： Eibl C,Grigoriu S,Hessenberger M,Wenger J,Puehringer S,Pinheiro AS,Wagner RN,Proell M,Reed JC,Page R,Diederichs K,Peti W

更新日期：2012-09-18 00:00:00

abstract：:The hairpin catalytic motif in tobacco ringspot virus satellite RNA consists of two helix-loop-helix elements on two adjacent arms of a four-way helical junction. The bases essential for catalytic activity are located in the loops that are brought into proximity by a conformational change as a prerequisite for catalys...

journal_title：Biochemistry

authors： Klostermeier D,Millar DP

更新日期：2001-09-18 00:00:00

abstract：:The mass-driven assembly of spectrin dimers to form tetramers involves two equal head-to-head alpha-beta associations and requires at least 30 degrees C for interconversion to occur readily. In this paper, the properties of tetramer formation were investigated using two complementary univalent peptides (the alpha I do...

journal_title：Biochemistry

authors： DeSilva TM,Peng KC,Speicher KD,Speicher DW

更新日期：1992-11-10 00:00:00

abstract：:Adriamycin plays a prominent role in the treatment of leukemia and solid tumors in man. The mode of interaction of adriamycin with its nuclear target, responsible for its therapeutic effect, is known [Berman, H. M., & Young, P.R. (1981) Annu. Rev. Biophys. Bioeng. 10, 87-114]. The planar anthracycline moiety of adriam...

journal_title：Biochemistry

authors： Goormaghtigh E,Brasseur R,Huart P,Ruysschaert JM

更新日期：1987-03-24 00:00:00

abstract：:The structure of a short-chain IgG2a antibody, which is a member of the family of mouse anti-dansyl switch variant antibodies with identical variable regions but different heavy-chain constant regions [Dangl, J.L., Parks, D. R., Oi, V. T., & Herzenberg, L. A. (1982) Cytometry 2, 395-401], is reported. Amino acid seque...

journal_title：Biochemistry

authors： Igarashi T,Sato M,Katsube Y,Takio K,Tanaka T,Nakanishi M,Arata Y

更新日期：1990-06-19 00:00:00

abstract：:Protein enzymes often use ionizable side chains, such as histidine, for general acid-base catalysis because the imidazole pK(a) is near neutral pH. RNA enzymes, on the other hand, are comprised of nucleotides which do not have apparent pK(a) values near neutral pH. Nevertheless, it has been recently shown that cytidin...

journal_title：Biochemistry

authors： Ravindranathan S,Butcher SE,Feigon J

更新日期：2000-12-26 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:The organization and interaction of chlorophylls (Chl) and the kinetics of the energy transfer in the core antenna of photosystem I (PSI) trimeric and monomeric complexes, isolated from Spirulina platensis with Triton X-100 have been studied by stationary and time-resolved fluorescence. At 295 K both complexes show an...

journal_title：Biochemistry

authors： Karapetyan NV,Dorra D,Schweitzer G,Bezsmertnaya IN,Holzwarth AR

更新日期：1997-11-11 00:00:00

abstract：:The temperature dependence of the hydrogen-tritium and deuterium-hydrogen exchange reactions in poly(DL-alanine) has been reexamined. The results indicate a significant contribution to the observed exchange rates from the water-catalyzed reaction at pD values near pDmin. The activation enthalpy for water-catalyzed deu...

journal_title：Biochemistry

authors： Gregory RB,Crabo L,Percy AJ,Rosenberg A

更新日期：1983-02-15 00:00:00

abstract：:Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...

journal_title：Biochemistry

authors： Pan KM,Damodaran S,Greaser ML

更新日期：1994-07-12 00:00:00

abstract：:HpaI and BphI are two pyruvate class II aldolases found in aromatic meta-cleavage degradation pathways that catalyze similar reactions but are not related in sequence. Steady-state kinetic analysis of the aldol addition reactions and product inhibition assays showed that HpaI exhibits a rapid equilibrium random order ...

journal_title：Biochemistry

authors： Wang W,Baker P,Seah SY

更新日期：2010-05-04 00:00:00

abstract：:Mitochondria act as a focal point for upstream apoptosis signals by releasing cytochrome c into the cytosol, leading to the activation of caspases and subsequent cell death. Members of the Bcl-2 protein family regulate this phenomenon by heterodimerization via the BH3 domain of proapoptotic members opposing their pro-...

journal_title：Biochemistry

authors： Moradi-Améli M,Lorca T,Ficheux D,di Pietro A,Gillet G

更新日期：2002-07-09 00:00:00

abstract：:The UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase, encoded by lpxA, catalyzes the first step of lipid A biosynthesis in Gram-negative bacteria, the (R)-3-hydroxyacyl-ACP-dependent acylation of the 3-OH group of UDP-GlcNAc. Recently, we demonstrated that the Arabidopsis thaliana orthologs of six enzymes of the b...

journal_title：Biochemistry

authors： Joo SH,Chung HS,Raetz CR,Garrett TA

更新日期：2012-05-29 00:00:00