Photoinduced reactivity of the HIV-1 envelope glycoprotein with a membrane-embedded probe reveals insertion of portions of the HIV-1 Gp41 cytoplasmic tail into the viral membrane.


:The interactions of HIV-1 Env (gp120-gp41) with CD4 and coreceptors trigger a barrage of conformational changes in Env that drive the membrane fusion process. Various regions of gp41 have profound effects on HIV entry and budding. However, the precise interactions between gp41 and the membrane have not been elucidated. To examine portions of membrane proteins that are embedded in membrane lipids, we have studied photoinduced chemical reactions in membranes using the lipid bilayer specific probe iodonaphthyl azide (INA). Here we show that in addition to the transmembrane anchor, amphipatic sequences in the cytoplasmic tail (CT) of HIV-1 gp41 are labeled by INA. INA labeling of the HIV-1 gp41 CT was similar whether wild-type or a mutant HIV-1 was used with uncleaved p55 Gag, which does not allow entry. These results shed light on the disposition of the HIV-1 gp41 CT with respect to the membrane. Moreover, our data have general implications for topology of membrane proteins and their in situ interactions with the lipid bilayer.






Viard M,Ablan SD,Zhou M,Veenstra TD,Freed EO,Raviv Y,Blumenthal R




Has Abstract


2008-02-19 00:00:00












  • Structure and mechanism of an ADP-glucose phosphorylase from Arabidopsis thaliana.

    abstract::The X-ray crystal structure of the At5g18200.1 protein has been determined to a nominal resolution of 2.30 A. The structure has a histidine triad (HIT)-like fold containing two distinct HIT-like motifs. The sequence of At5g18200.1 indicates a distant family relationship to the Escherichia coli galactose-1-P uridylyltr...


    pub_type: 杂志文章


    authors: McCoy JG,Arabshahi A,Bitto E,Bingman CA,Ruzicka FJ,Frey PA,Phillips GN Jr

    更新日期:2006-03-14 00:00:00

  • Yeast alcohol dehydrogenase structure and catalysis.

    abstract::Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues. A structure for ADH1 was determined by X-ray crystallography at 2.4 Å resolution. The asym...


    pub_type: 杂志文章


    authors: Raj SB,Ramaswamy S,Plapp BV

    更新日期:2014-09-16 00:00:00

  • Organellar Na+/H+ exchangers: novel players in organelle pH regulation and their emerging functions.

    abstract::Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...


    pub_type: 杂志文章,评审


    authors: Ohgaki R,van IJzendoorn SC,Matsushita M,Hoekstra D,Kanazawa H

    更新日期:2011-02-01 00:00:00

  • DNA damage and cytotoxicity induced by minor groove binding methyl sulfonate esters.

    abstract::Minor groove specific DNA equilibrium binding peptides (lex) based on N-methylpyrrole-carboxamide and/or N-methylimidazolecarboxamide subunits have been modified with an O-methyl sulfonate ester functionality to target DNA methylation in the minor groove at Ade/Thy- and/or Gua/Cyt-rich sequences. HPLC and sequencing g...


    pub_type: 杂志文章


    authors: Varadarajan S,Shah D,Dande P,Settles S,Chen FX,Fronza G,Gold B

    更新日期:2003-12-09 00:00:00

  • Three small ribooligonucleotides with specific arginine sites.

    abstract::Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge...


    pub_type: 杂志文章


    authors: Connell GJ,Illangesekare M,Yarus M

    更新日期:1993-06-01 00:00:00

  • Isolation and characterization of papaya peptidase A from commercial chymopapain.

    abstract::Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...


    pub_type: 杂志文章


    authors: Robinson GW

    更新日期:1975-08-12 00:00:00

  • Phosphorescence studies of the interaction of myelin basic protein with phosphatidylserine vesicles.

    abstract::Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...


    pub_type: 杂志文章


    authors: Vadas EB,Melançon P,Braun PE,Galley WC

    更新日期:1981-05-26 00:00:00

  • Zebrafish neuroglobin is a cell-membrane-penetrating globin.

    abstract::Neuroglobin (Ngb) is a recently discovered vertebrate heme protein that is expressed in the brain and can reversibly bind oxygen. Mammalian Ngb is involved in neuroprotection under oxidative stress conditions, such as ischemia and reperfusion. We previously demonstrated that human ferric Ngb binds to the alpha subunit...


    pub_type: 杂志文章


    authors: Watanabe S,Wakasugi K

    更新日期:2008-05-13 00:00:00

  • Collagen biosynthesis by cultured Chinese hamster lung cells. Cell-free synthesis of procollagen alpha chains.

    abstract::Cell-free extracts from the HTl clone of cultured Chinese hamster lung cells efficiently promote the incorporation of proline into newly synthesized material, 50% of which is digestible to small peptides by highly purified bacterial collagenase. The synthesis of the these products occurs under optimal protein synthesi...


    pub_type: 杂志文章


    authors: Haralson MA,Frey KL,Mitchell WM

    更新日期:1978-03-07 00:00:00

  • A transforming growth factor-alpha-Pseudomonas exotoxin hybrid protein undergoes pH-dependent conformational changes conducive to membrane interaction.

    abstract::TP40 is a chimeric protein containing transforming growth factor alpha (TGF-alpha) at the N-terminus and a derivative of a 40,000-Da segment (PE40 delta cys) of Pseudomonas exotoxin (PE). PE40 delta cys contains domains Ib, II, and III of PE in which the cysteines are mutated to alanines. The rationale for inclusion o...


    pub_type: 杂志文章


    authors: Sanyal G,Marquis-Omer D,Gress JO,Middaugh CR

    更新日期:1993-04-06 00:00:00

  • Comparative structural study of the N-linked oligosaccharides of human normal and pathological immunoglobulin G.

    abstract::The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...


    pub_type: 杂志文章


    authors: Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

    更新日期:1987-02-24 00:00:00

  • Physical and conformational properties of synthetic idealized signal sequences parallel their biological function.

    abstract::Transported proteins often contain an extension sequence called the signal peptide. The alkaline phosphatase (PhoA) signal sequence represents a typical signal peptide for comparison to idealized sequences both in vivo and in vitro. We have designed a series of idealized signal sequences which vary in amino terminal c...


    pub_type: 杂志文章


    authors: Izard JW,Doughty MB,Kendall DA

    更新日期:1995-08-08 00:00:00

  • Three-dimensional structure of N5-carboxyaminoimidazole ribonucleotide synthetase: a member of the ATP grasp protein superfamily.

    abstract::Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....


    pub_type: 杂志文章


    authors: Thoden JB,Kappock TJ,Stubbe J,Holden HM

    更新日期:1999-11-23 00:00:00

  • Chloroplasts biogenesis: detection of divinylprotochlorophyllide ester in higher plants.

    abstract::It is shown that the protochlorophyllide ester pool of etiolated higher plants is a faithful copy of the protochlorophyllide pool. It is made up of both monovinyl- and divinylprotochlorophyllide esters. Although the two tetrapyrroles exhibited similar emission maxima, they were distinguishable by their Soret excitatio...


    pub_type: 杂志文章


    authors: Belanger FC,Rebeiz CA

    更新日期:1980-10-14 00:00:00

  • Thermodynamics of transient conformations in the folding pathway of barnase: reorganization of the folding intermediate at low pH.

    abstract::New classes of small proteins have recently been found that refold rapidly with two-state kinetics from a substantially unfolded conformation ("U") and without the accumulation of a folding intermediate. Barnase, on the other hand, is representative of a class of proteins that display multistate kinetics and refold fr...


    pub_type: 杂志文章


    authors: Oliveberg M,Fersht AR

    更新日期:1996-02-27 00:00:00

  • Dihydrofolate reductase from Escherichia coli: probing the role of aspartate-27 and phenylalanine-137 in enzyme conformation and the binding of NADPH.

    abstract::In the absence of ligands, dihydrofolate reductase from Escherichia coli exists in at least two interconvertible conformations, only one of which binds NADPH with high affinity. This equilibrium is pH dependent, involving an ionizable group of the enzyme (pK approximately 5.5), and the proportion of the NADPH-binding ...


    pub_type: 杂志文章


    authors: Dunn SM,Lanigan TM,Howell EE

    更新日期:1990-09-18 00:00:00

  • A photolabile oligodeoxyribonucleotide probe of the peptidyltransferase center: identification of neighboring ribosomal components.

    abstract::In this work we report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring its target site in 23S rRNA. The probe is complementary to 23S rRNA nucleotides 2497-2505, a single-stranded sequence that has been shown to...


    pub_type: 杂志文章


    authors: Muralikrishna P,Cooperman BS

    更新日期:1991-06-04 00:00:00

  • Development of a neutralizing antibody specific for the active form of matrix metalloproteinase-13.

    abstract::Matrix metalloproteinase-13 (MMP-13) is important in the pathology of osteoarthritis (OA). Although MMP-13 is considered a therapeutic target for OA, it is unclear how MMP-13 activity is regulated by the system that comprises various proteinases and their inhibitors. MMP-13 neutralizing antibodies could be a useful to...


    pub_type: 杂志文章


    authors: Naito S,Takahashi T,Onoda J,Yamauchi A,Kawai T,Kishino J,Yamane S,Fujii I,Fukui N,Numata Y

    更新日期:2012-11-06 00:00:00

  • Cholesterol efflux from macrophage foam cells is enhanced by active phospholipid transfer protein through generation of two types of acceptor particles.

    abstract::Phospholipid transfer protein (PLTP) is expressed by macrophage-derived foam cells in human atherosclerotic lesions, suggesting a regulatory role for PLTP in cellular cholesterol homeostasis. However, the exact role of PLTP in the reverse cholesterol transport pathway is not known. PLTP is present in plasma as two for...


    pub_type: 杂志文章


    authors: Vikstedt R,Metso J,Hakala J,Olkkonen VM,Ehnholm C,Jauhiainen M

    更新日期:2007-10-23 00:00:00

  • Rat organic anion transporting protein 1A1 (Oatp1a1): purification and phosphopeptide assignment.

    abstract::Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...


    pub_type: 杂志文章


    authors: Xiao Y,Nieves E,Angeletti RH,Orr GA,Wolkoff AW

    更新日期:2006-03-14 00:00:00

  • Small molecular, macromolecular, and cellular chloramines react with thiocyanate to give the human defense factor hypothiocyanite.

    abstract::Thiocyanate reacts noncatalytically with myeloperoxidase-derived HOCl to produce hypothiocyanite (OSCN(-)), thereby potentially limiting the propensity of HOCl to inflict host tissue damage that can lead to inflammatory diseases. However, the efficiency with which SCN(-) captures HOCl in vivo depends on the concentrat...


    pub_type: 杂志文章


    authors: Xulu BA,Ashby MT

    更新日期:2010-03-09 00:00:00

  • Thermal stability of membrane-reconstituted yeast cytochrome c oxidase.

    abstract::The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...


    pub_type: 杂志文章


    authors: Morin PE,Diggs D,Freire E

    更新日期:1990-01-23 00:00:00

  • Purification and characterization of two new high molecular weight forms of DNA polymerase delta.

    abstract::Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...


    pub_type: 杂志文章


    authors: Crute JJ,Wahl AF,Bambara RA

    更新日期:1986-01-14 00:00:00

  • Dihydropyrimidinone positive modulation of delta-subunit-containing gamma-aminobutyric acid type A receptors, including an epilepsy-linked mutant variant.

    abstract::Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...


    pub_type: 杂志文章


    authors: Lewis RW,Mabry J,Polisar JG,Eagen KP,Ganem B,Hess GP

    更新日期:2010-06-15 00:00:00

  • Electrostatic optimization of the conformational energy landscape in a metamorphic protein.

    abstract::The equilibrium unfolding reaction of Ltn, a metamorphic C-class chemokine, was monitored by tryptophan fluorescence to determine unfolding free energies. Measurements revealed that addition of 150 mM NaCl stabilized the Ltn chemokine fold by approximately 1 kcal/mol. Specific mutations involving Arg23 and Arg43 also ...


    pub_type: 杂志文章


    authors: Tyler RC,Wieting JC,Peterson FC,Volkman BF

    更新日期:2012-11-13 00:00:00

  • Sugar recognition by CscB and LacY.

    abstract::The sucrose permease (CscB) and lactose permease (LacY) of Escherichia coli belong to the oligosaccharide/H(+) symporter subfamily of the major facilitator superfamily, and both catalyze sugar/H(+) symport across the cytoplasmic membrane. Thus far, there is no common substrate for the two permeases; CscB transports su...


    pub_type: 杂志文章


    authors: Sugihara J,Smirnova I,Kasho V,Kaback HR

    更新日期:2011-12-27 00:00:00

  • Substrate specificity and sequence preference of G:T mismatch repair: incision at G:T, O6-methylguanine:T, and G:U mispairs in DNA by human cell extracts.

    abstract::Extracts of two human glioma cell lines (lacking O6-methylguanine DNA-methyltransferase) (i.e., A1235 and its alkylation-resistant derivative A1235-MR4) were examined for their ability to execute strand incision at different base mismatches in model (45-bp) DNA. These heteroduplex substrates were of the same sequence ...


    pub_type: 杂志文章


    authors: Lari SU,Al-Khodairy F,Paterson MC

    更新日期:2002-07-23 00:00:00

  • Cobalt-cytochrome c. I. Preparation, properties, and enzymic activity.

    abstract::An improved procedure for the preparation of cobalt-cytochrome c has been developed. Various factors influencing the cobalt insertion process are discussed. The optical spectra of cobalt-cytochrome c suggest a six-coordinated species. The spectral shifts occurring with oxidation-reduction are compared with those obser...


    pub_type: 杂志文章


    authors: Dickinson LC,Chien JC

    更新日期:1975-08-12 00:00:00

  • Fourier transform Raman spectroscopy of the bacteriorhodopsin mutant Tyr-185-->Phe: formation of a stable O-like species during light adaptation and detection of its transient N-like photoproduct.

    abstract::Near-infrared FT-Raman spectroscopy can be used to measure the vibrations of the bacteriorhodopsin (bR) chromophore without the disadvantage of conventional visible resonance Raman spectroscopy, where the visible excitation drives the bR photoreactions. We utilized this technique to investigate the light-dark adaptati...


    pub_type: 杂志文章


    authors: Rath P,Krebs MP,He Y,Khorana HG,Rothschild KJ

    更新日期:1993-03-09 00:00:00

  • An engineered disulfide cross-link accelerates the refolding rate of calcium-free subtilisin by 850-fold.

    abstract::The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...


    pub_type: 杂志文章


    authors: Strausberg S,Alexander P,Wang L,Gallagher T,Gilliland G,Bryan P

    更新日期:1993-10-05 00:00:00