Purification of botrocetin from Bothrops jararaca venom. Analysis of the botrocetin-mediated interaction between von Willebrand factor and the human platelet membrane glycoprotein Ib-IX complex.

abstract：:A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...

journal_title：Biochemistry

authors： Cappai R,Stewart L,Jobling MF,Thyer JM,White AR,Beyreuther K,Collins SJ,Masters CL,Barrow CJ

更新日期：1999-03-16 00:00:00

abstract：:CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...

journal_title：Biochemistry

authors： Owen GR,Channell JA,Forsyth VT,Haertlein M,Mitchell EP,Capovilla A,Papathanasopoulos M,Cerutti NM

更新日期：2016-04-19 00:00:00

abstract：:Escherichia coli tyrosine kinase (Etk) regulates the export of pathogenic capsular polysaccharide (CPS) by intermolecularly autophosphorylating its C-terminal tyrosine cluster. The kinase Etk, however, needs to be first activated by the intramolecular phosphorylation of a tyrosine residue, Y574, next to the active sit...

journal_title：Biochemistry

authors： Lu T,Tan H,Lee D,Chen G,Jia Z

更新日期：2009-08-25 00:00:00

abstract：:In the cyanobacterium Anabaena PCC7120, the precursor to tRNA(Leu) contains a 249-nucleotide group I intron that undergoes efficient self-splicing in vitro. By deleting the 5' and 3' splice sites, this intron has now been converted to an RNA enzyme that uses a guanosine nucleophile to cleave substrate RNAs (S) with mu...

journal_title：Biochemistry

authors： Zaug AJ,Dávila-Aponte JA,Cech TR

更新日期：1994-12-13 00:00:00

abstract：:We have combined equilibrium and steered molecular dynamics (SMD) simulations with principal component and correlation analyses to probe the mechanism of allosteric regulation in imidazole glycerol phosphate (IGP) synthase. An evolutionary analysis of IGP synthase revealed a conserved network of interactions leading f...

journal_title：Biochemistry

authors： Amaro RE,Sethi A,Myers RS,Davisson VJ,Luthey-Schulten ZA

更新日期：2007-02-27 00:00:00

abstract：:We prepared a series of cryptogein mutants, an elicitor from Phytophthora cryptogea, with altered abilities to bind sterols and fatty acids. The induction of the early events, i.e., synthesis of active oxygen species and pH changes, in suspension tobacco cells by these mutated proteins was proportional to their abilit...

journal_title：Biochemistry

authors： Lochman J,Kasparovsky T,Damborsky J,Osman H,Marais A,Chaloupkova R,Ponchet M,Blein JP,Mikes V

更新日期：2005-05-03 00:00:00

abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

journal_title：Biochemistry

authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

更新日期：1989-05-30 00:00:00

abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...

journal_title：Biochemistry

authors： Chryssomallis GS,Torgerson PM,Drickamer HG,Weber G

更新日期：1981-07-07 00:00:00

abstract：:We describe a series of indolequinones as efficient mechanism-based inhibitors of NRH:quinone oxidoreductase 2 (NQO2) for use either in cellular or cell-free systems. Compounds were designed to be reduced in the active site of the enzyme leading to loss of a substituted phenol leaving group and generation of a reactiv...

journal_title：Biochemistry

authors： Yan C,Dufour M,Siegel D,Reigan P,Gomez J,Shieh B,Moody CJ,Ross D

更新日期：2011-08-09 00:00:00

abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

journal_title：Biochemistry

authors： Sastry SS,Buki KG,Kun E

更新日期：1989-06-27 00:00:00

abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...

journal_title：Biochemistry

authors： McCutchen SL,Colon W,Kelly JW

更新日期：1993-11-16 00:00:00

abstract：:We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration from 100 to 500 mM, via replacement of potassium...

journal_title：Biochemistry

authors： Chen Y,Erickson HP

更新日期：2009-07-21 00:00:00

abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...

journal_title：Biochemistry

authors： Cho YK,Rebholz KL,Northrop DB

更新日期：1994-08-16 00:00:00

abstract：:The fatty acid synthase (FAS) of animal tissue is a dimer of two identical subunits, each with a Mr of 260,000. The subunit is a single multifunctional protein having seven catalytic activities and a site for binding of the prosthetic group 4'-phosphopantetheine. The mRNA coding for the subunit has an estimated size o...

journal_title：Biochemistry

authors： Kasturi R,Chirala S,Pazirandeh M,Wakil SJ

更新日期：1988-10-04 00:00:00

abstract：:The crystal structure of the catalytic domain of rat DNA polymerase beta revealed that Asp256 is located in proximity to the previously identified active site residues Asp190 and Asp192. We have prepared and kinetically characterized the nucleotidyl transfer activity of wild type and several mutant forms of human and ...

journal_title：Biochemistry

authors： Menge KL,Hostomsky Z,Nodes BR,Hudson GO,Rahmati S,Moomaw EW,Almassy RJ,Hostomska Z

更新日期：1995-12-12 00:00:00

abstract：:N-RAP is a recently discovered muscle-specific protein found at cardiac intercalated disks. Double immunogold labeling of mouse cardiac muscle reveals that vinculin is located immediately adjacent to the fascia adherens region of the intercalated disk membrane, while N-RAP extends approximately 100 nm further toward t...

journal_title：Biochemistry

authors： Zhang JQ,Elzey B,Williams G,Lu S,Law DJ,Horowits R

更新日期：2001-12-11 00:00:00

abstract：:In an effort to characterize the bimolecular interface between parathyroid hormone (PTH) and its human receptor PTH1-Rc (hPTH1-Rc), we previously identified two contact sites in the receptor: one for position 1 and another for position 13 (located at the ends of the principal activation domain) in PTH(1-34). The prese...

journal_title：Biochemistry

authors： Greenberg Z,Bisello A,Mierke DF,Rosenblatt M,Chorev M

更新日期：2000-07-18 00:00:00

abstract：:Monomeric sarcosine oxidase (MSOX) binds the L-proline zwitterion (pKa = 10.6). The reactive substrate anion is generated by ionization of the ES complex (pKa = 8.0). Tyr317 was mutated to Phe to determine whether this step might involve proton transfer to an active site base. The mutation does not eliminate the ioniz...

journal_title：Biochemistry

authors： Zhao G,Jorns MS

更新日期：2005-12-27 00:00:00

abstract：:How various anti-amyloidogenic compounds inhibit the formation of Alzheimer's beta-amyloid fibrils (fAbeta) from amyloid beta-peptide (Abeta) and destabilize fAbeta remains poorly understood. Using spectrophotometry, spectrofluorometry, atomic force microscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis...

journal_title：Biochemistry

authors： Hirohata M,Hasegawa K,Tsutsumi-Yasuhara S,Ohhashi Y,Ookoshi T,Ono K,Yamada M,Naiki H

更新日期：2007-02-20 00:00:00

abstract：:The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

journal_title：Biochemistry

authors： Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

更新日期：1997-03-25 00:00:00

abstract：:The effect of Mg2+ on the disposition of myosin cross-bridges was studied on myofibrils and synthetic myosin and rod filaments by employing chymotryptic digestion and chemical cross-linking methods. In the presence of low Mg2+ concentrations (0.1 mM), the proteolytic susceptibility at the heavy meromyosin/light meromy...

journal_title：Biochemistry

authors： Reisler E,Liu J,Cheung P

更新日期：1983-10-11 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is a transmembrane protein involved in cell signaling processes, and dysregulation of its activity often drives tumor growth. EGFR is a clinically validated tumor marker and target for antibodies and tyrosine kinase inhibitors. We demonstrate that a fusion protein of the nat...

journal_title：Biochemistry

authors： Feiner RC,Pennè I,Müller B,Müller KM

更新日期：2019-02-26 00:00:00

abstract：:We report here on the Raman spectra of NADH, 3-acetylpyridine adenine dinucleotide, APAD+, and a fragment of these molecules, adenosine 5'-diphosphate ribose (ADPR) bound to the mitochondrial (mMDH) and cytoplasmic (or soluble, sMDH) forms of malate dehydrogenase. We observe changes in the Raman spectrum of the adenos...

journal_title：Biochemistry

authors： Deng H,Burgner J,Callender R

更新日期：1991-09-10 00:00:00

abstract：:The iron heme and its immediate environment can provide information that is pivotal to our understanding of the structural and mechanistic features that confer unusual properties to the heme peroxidases. X-ray absorption spectroscopy (XAS), which is ideally suited for the investigation of the local environment and ele...

journal_title：Biochemistry

authors： Sinclair R,Copeland B,Yamazaki I,Powers L

更新日期：1995-10-10 00:00:00

abstract：:Human DNA primase synthesizes short RNA primers that DNA polymerase alpha then elongates during the initiation of all new DNA strands. Even though primase misincorporates NTPs at a relatively high frequency, this likely does not impact the final DNA product since the RNA primer is replaced with DNA. We used an extensi...

journal_title：Biochemistry

authors： Urban M,Joubert N,Purse BW,Hocek M,Kuchta RD

更新日期：2010-02-02 00:00:00

abstract：:The selenoamino acids methylselenocysteine (MeSeCys) and selenomethionine (SeMet) have disparate efficacies as anticancer agents. Herein, we use X-ray absorption spectroscopy to determine the chemical form of selenium in human neuroblastoma cells. Cells treated with MeSeCys contain a significant diselenide component, ...

journal_title：Biochemistry

authors： Weekley CM,Aitken JB,Musgrave IF,Harris HH

更新日期：2012-01-24 00:00:00

abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

journal_title：Biochemistry

authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

更新日期：2009-10-27 00:00:00

abstract：:The dimeric nature of the HIV protease has been exploited to devise a novel mode of inhibiting the enzyme. The use of defective monomers or nonidentical subunits to exchange with wild-type homodimers produces catalytically defective heterodimers. Incubation of the HIV1 or HIV2 protease with a 4-fold molar excess of an...

journal_title：Biochemistry

authors： Babé LM,Pichuantes S,Craik CS

更新日期：1991-01-08 00:00:00

abstract：:Atomic force microscopy (AFM) is a technique widely used to image protein-DNA complexes, and its application has now been extended to the measurements of protein-DNA binding constants and specificities. However, the spreading of the protein-DNA complexes on a flat substrate, generally mica, is required prior to AFM im...

journal_title：Biochemistry

authors： Sorel I,Piétrement O,Hamon L,Baconnais S,Cam EL,Pastré D

更新日期：2006-12-12 00:00:00

abstract：:Calmodulin, the Ca(2+)-dependent activator of many cellular processes, contains two well-defined structural domains, each of which binds two Ca(2+) ions. In its Ca(2+)-free (apo) form, it provides an attractive model for studying mechanisms of protein unfolding, exhibiting two separable, reversible processes, indicati...

journal_title：Biochemistry

authors： Biekofsky RR,Martin SR,McCormick JE,Masino L,Fefeu S,Bayley PM,Feeney J

更新日期：2002-05-28 00:00:00